Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats

Muaz BELVİRANLI, (Selçuk Üniversitesi, Tıp Fakültesi, Psikiyatri Anabilim Dalı, Konya, Türkiye)
Sadık BÜYÜKBAŞ, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Biyokimya Anabilim Dalı, Konya, Türkiye)
Hakkı GÖKBEL, (Selçuk Üniversitesi, Tıp Fakültesi, Psikiyatri Anabilim Dalı, Konya, Türkiye)
Nilsel OKUDAN (Selçuk Üniversitesi, Tıp Fakültesi, Psikiyatri Anabilim Dalı, Konya, Türkiye)
Turkish Journal of Medical Sciences
2 0
Yıl: 2015 Cilt: 45 Sayı: 3 Sayfa Aralığı: 489 - 495 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats

Öz:
Background/aim: To evaluate the effects of grape seed extract (GSE) supplementation on oxidative stress and antioxidant markers in streptozotocin (STZ)-induced diabetic rats. Materials and methods: Thirty-six male rats were divided into the following four groups: control, GSE-supplemented control, diabetic, and GSE-supplemented diabetic. Beginning on day 7 after STZ injection, the rats were administered GSE (100 mg kg-1 day-1) in drinking water for 6 weeks. At the end of week 6, rats were sacrificed by cardiac puncture. Plasma nitric oxide (NO) levels and xanthine oxidase (XO), adenosine deaminase (ADA), and glutathione peroxidase (GPx) activities were analyzed. Results: Both XO and ADA activities increased and NO levels decreased in diabetic rats (P < 0.05). GSE supplementation normalized all of these changes. Antioxidant enzyme activities decreased in diabetic rats compared to the controls (P < 0.05). GSE supplementation increased antioxidant enzyme activities in both diabetic and healthy rats (P < 0.05). Conclusion: These findings suggest that 6 weeks of oral GSE supplementation may prevent oxidative stress and improve antioxidant status in diabetic rats.
Anahtar Kelime:

Konular: Cerrahi
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • West IC. Radicals and oxidative stress in diabetes. Diabet Med 2000; 17: 171‒180.
  • Kuroki T, Isshiki K, King GL. Oxidative stress: the lead or supporting actor in the pathogenesis of diabetic complications. J Am Soc Nephrol 2003; 14: S216‒220.
  • Baynes JW, Thorpe SR. Role of oxidative stress in diabetic complications: a new perspective on an old paradigm. Diabetes 1999; 48: 1‒9.
  • Maritim AC, Sanders RA, Watkins JB 3rd. Diabetes, oxidative stress, and antioxidants: a review. J Biochem Mol Toxicol 2003; 17: 24‒38.
  • Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother 2005; 59: 365‒373.
  • Urso ML, Clarkson PM. Oxidative stress, exercise, and antioxidant supplementation. Toxicology 2003; 189: 41‒54.
  • Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radic Biol Med 1996; 20: 933‒956.
  • Bagchi D, Bagchi M, Stohs S, Ray SD, Sen CK, Preuss HG. Cellular protection with proanthocyanidins derived from grape seeds. Ann NY Acad Sci 2002; 957: 260‒270.
  • Shahidi F, Wanasundara PK. Phenolic antioxidants. Crit Rev Food Sci Nutr 1992; 32: 67‒103.
  • Salah N, Miller NJ, Paganga G, Tijburg L, Bolwell GP, Rice- Evans C. Polyphenolic flavanols as scavengers of aqueous phase radicals and as chain-breaking antioxidants. Arch Biochem Biophys 1995; 322: 339‒346.
  • Bagchi D, Garg A, Krohn RL, Bagchi M, Tran MX, StohsOxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res Commun Mol Pathol Pharmacol 1997; 95: 179‒189. SJ.
  • Pinent M, Blay M, Bladé MC, Salvadó MJ, Arola L, Ardévol A. Grape seed-derived procyanidins have an antihyperglycemic effect in streptozotocin-induced diabetic rats and insulinomimetic activity in insulin-sensitive cell lines. Endocrinology 2004; 145: 4985‒4990.
  • El-Alfy AT, Ahmed AA, Fatani AJ. Protective effect of red grape seeds proanthocyanidins against induction of diabetes by alloxan in rats. Pharmacol Res 2005; 52: 264‒270.
  • Cheng M, Gao HQ, Xu L, Li BY, Zhang H, Li XH. Cardioprotective effects of grape seed proanthocyanidins extracts in streptozocin induced diabetic rats. J Cardiovasc Pharmacol 2007; 50: 503‒509.
  • Joshi SS, Kuszynski CA, Bagchi M, Bagchi D. Chemopreventive effects of grape seed proanthocyanidin extract on Chang liver cells. Toxicology 2000; 155: 83‒90.
  • Kalfin R, Righi A, Del Rosso A, Bagchi D, Generini S, Cerinic MM, Das DK. Activin, a grape seed-derived proanthocyanidin extract, reduces plasma levels of oxidative stress and adhesion molecules (ICAM-1, VCAM-1 and E-selectin) in systemic sclerosis. Free Radic Res 2002; 36: 819‒825.
  • Sen CK, Bagchi D. Regulation of inducible adhesion molecule expression in human endothelial cells by grape seed proanthocyanidin extract. Mol Cell Biochem 2001; 216: 1‒7.
  • Wang RN, Bouwens L, Klöppel G. Beta-cell proliferation in normal and streptozotocin-treated newborn rats: site, dynamics and capacity. Diabetologia 1994; 37: 1088‒1096.
  • Balu M, Sangeetha P, Murali G, Panneerselvam C. Modulatory role of grape seed extract on age-related oxidative DNA damage in central nervous system of rats. Brain Res Bullet 2006; 68: 469‒473.
  • Sharp PE, La Regina MC. Important biological functions. In: Suckow MA, editor. The Laboratory Rat. Boca Raton, FL, USA: CRC Press; 1998. p. 17.
  • Prajda N, Weber G. Malignant transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 1975; 59: 245‒249.
  • Giusti G. Adenosine deaminase. In: Bergmeyer MV, editor. Methods of Enzymatic Analysis. 2nd ed. New York, NY, USA: Academic Press; 1974. pp. 1092–1098.
  • Cortas NK, Wakid NW. Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. Clin Chem 1990; 36: 1440‒1443.
  • Cui XP, Li BY, Gao HQ, Wei N, Wang WL, Lu M. Effects of grape seed proanthocyanidin extracts on peripheral nerves in streptozocin-induced diabetic rats. J Nutr Sci Vitaminol (Tokyo) 2008; 54: 321‒328.
  • Hwang IK, Kim DW, Park JH, Lim SS, Yoo KY, Kwon DY, Kim DW, Moon WK, Won MH. Effects of grape seed extract and its ethylacetate/ethanol fraction on blood glucose levels in a model of type 2 diabetes. Phytother Res 2009; 23: 1182‒1185.
  • Al-Awwadi N, Azay J, Poucheret P, Cassanas G, Krosniak M, Auger C, Gasc F, Rouanet JM, Cros G, Teissèdre PL. Antidiabetic activity of red wine polyphenolic extract, ethanol, or both in streptozotocin-treated rats. J Agric Food Chem 2004; 52: 1008‒1016.
  • Zhang Z, Li BY, Li XL, Cheng M, Yu F, Lu WD, Cai Q, Wang JF, Zhou RH, Gao HQ et al. Proteomic analysis of kidney and protective effects of grape seed procyanidin B2 in db/db mice indicate MFG-E8 as a key molecule in the development of diabetic nephropathy. Biochim Biophys Acta 2013; 1832: 805‒816.
  • Cay M, Naziroğlu M, Simsek H, Aydilek N, Aksakal M, Demirci M. Effects of intraperitoneally administered vitamin C on antioxidative defense mechanism in rats with diabetes induced by streptozotocin. Res Exp Med (Berl) 2001; 200: 205‒213.
  • Feillet-Coudray C, Rock E, Coudray C, Grzelkowska K, Azais- Braesco V, Dardevet D, Mazur A. Lipid peroxidation and antioxidant status in experimental diabetes. Clin Chim Acta 1999; 284: 31‒43.
  • Griesmacher A, Kindhauser M, Andert SE, Schreiner W, Toma C, Knoebl P, Pietschmann P, Prager R, Schnack C, Schernthaner G. Enhanced serum levels of thiobarbituric-acid-reactive substances in diabetes mellitus. Am J Med 1995; 98: 469‒475.
  • Ruiz C, Alegría A, Barberá R, Faré R, Lagarda MJ. Lipid peroxidation and antioxidant enzyme activities in patients with type 1 diabetes mellitus. Scand J Clin Lab Invest 1999; 59: 99‒105.
  • Ates O, Cayli SR, Yucel N, Altinoz E, Kocak A, Durak MA, Turkoz Y, Yologlu S. Central nervous system protection by resveratrol in streptozotocin-induced diabetic rats. J Clin Neurosci 2007; 14: 256‒260.
  • Celik S, Erdogan S. Caffeic acid phenethyl ester (CAPE) protects brain against oxidative stress and inflammation induced by diabetes in rats. Mol Cell Biochem 2008; 312: 39‒46.
  • Hoshino T, Yamada K, Masuoka K, Tsuboi I, Itoh K, Nonaka K, Oizumi K. Elevated adenosine deaminase activity in the serum of patients with diabetes mellitus. Diabetes Res Clin Pract 1994; 25: 97‒102.
  • Kurtul N, Pence S, Akarsu E, Kocoglu H, Aksoy Y, Aksoy H. Adenosine deaminase activity in the serum of type 2 diabetic patients. Acta Medica (Hradec Kralove) 2004; 47: 33‒35.
  • Mokhtari M, Hashemi M, Yaghmaei M, Molashahi F, Shikhzadeh A, Niazi A, Ghavami S. Serum adenosine deaminase activity in gestational diabetes mellitus and normal pregnancy. Arch Gynecol Obstet 2010; 281: 1148‒1153.
  • Singh LS, Sharma R. Alloxan diabetes regulates adenosine deaminase activity in mice: tissue- and age-specific correlation. Biochem Mol Biol Int 1998; 46: 55‒61.
  • Ozyurt B, Iraz M, Koca K, Ozyurt H, Sahin S. Protective effects of caffeic acid phenethyl ester on skeletal muscle ischemia- reperfusion injury in rats. Mol Cell Biochem 2006; 292: 197‒203.
  • Ding Y, Dai X, Jiang Y, Zhang Z, Li Y. Functional and morphological effects of grape seed proanthocyanidins on peripheral neuropathy in rats with type 2 diabetes mellitus. Phytother Res 2014; 28: 1082–1087.
  • Ding Y, Dai X, Zhang Z, Jiang Y, Ma X, Cai X, Li Y. Proanthocyanidins protect against early diabetic peripheral neuropathy by modulating endoplasmic reticulum stress. J Nutr Biochem 2014; 25: 765‒772.
  • Wink DA, Mitchell JB. Chemical biology of nitric oxide: Insights into regulatory, cytotoxic, and cytoprotective mechanisms of nitric oxide. Free Radic Biol Med 1998; 25: 434‒456.
  • Ali MM, Agha FG. Amelioration of streptozotocin-induced diabetes mellitus, oxidative stress and dyslipidemia in rats by tomato extract lycopene. Scand J Clin Lab Invest 2009; 69: 371‒379.
  • Santilli F, Cipollone F, Mezzetti A, Chiarelli F. The role of nitric oxide in the development of diabetic angiopathy. Horm Metab Res 2004; 36: 319‒335.
  • Roychowdhury S, Wolf G, Keilhoff G, Bagchi D, Horn T. Protection of primary glial cells by grape seed proanthocyanidin extract against nitrosative/oxidative stress. Nitric Oxide 2001; 5: 137‒149.
  • Shao ZH, Hsu CW, Chang WT, Waypa GB, Li J, Li D, Li CQ, Anderson T, Qin Y, Schumacker PT et al. Cytotoxicity induced by grape seed proanthocyanidins: role of nitric oxide. Cell Biol Toxicol 2006; 22: 149‒158.
  • Okudan N, Barışkaner H, Gökbel H, Şahin AS, Belviranlı M, Baysal H. The effect of supplementation of grape seed proanthocyanidin extract on vascular dysfunction in experimental diabetes. J Med Food 2011; 14: 1298‒1302.
  • Laight DW, Carrier MJ, Anggård EE. Antioxidants, diabetes and endothelial dysfunction. Cardiovasc Res 2000; 47: 457‒464.
  • Abou-Seif MA, Youssef AA. Evaluation of some biochemical changes in diabetic patients. Clin Chim Acta 2004; 346: 161‒170.
  • Vessby J, Basu S, Mohsen R, Berne C, Vessby B. Oxidative stress and antioxidant status in type 1 diabetes mellitus. J Intern Med 2002; 251: 69‒76.
  • Kedziora-Kornatowska KZ, Luciak M, Paszkowski J. Lipid peroxidation and activities of antioxidant enzymes in the diabetic kidney: effect of treatment with angiotensin convertase inhibitors. IUBMB Life 2000; 49: 303‒307.
  • Kinalski M, Sledziewski A, Telejko B, Zarzycki W, Kinalska I. Lipid peroxidation and scavenging enzyme activity in streptozotocin-induced diabetes. Acta Diabetol 2000; 37: 179‒183.
APA Belviranli M, BÜYÜKBAŞ S, GÖKBEL H, OKUDAN N (2015). Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. , 489 - 495.
Chicago Belviranli Muaz,BÜYÜKBAŞ Sadık,GÖKBEL Hakkı,OKUDAN Nilsel Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. (2015): 489 - 495.
MLA Belviranli Muaz,BÜYÜKBAŞ Sadık,GÖKBEL Hakkı,OKUDAN Nilsel Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. , 2015, ss.489 - 495.
AMA Belviranli M,BÜYÜKBAŞ S,GÖKBEL H,OKUDAN N Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. . 2015; 489 - 495.
Vancouver Belviranli M,BÜYÜKBAŞ S,GÖKBEL H,OKUDAN N Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. . 2015; 489 - 495.
IEEE Belviranli M,BÜYÜKBAŞ S,GÖKBEL H,OKUDAN N "Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats." , ss.489 - 495, 2015.
ISNAD Belviranli, Muaz vd. "Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats". (2015), 489-495.
APA Belviranli M, BÜYÜKBAŞ S, GÖKBEL H, OKUDAN N (2015). Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences, 45(3), 489 - 495.
Chicago Belviranli Muaz,BÜYÜKBAŞ Sadık,GÖKBEL Hakkı,OKUDAN Nilsel Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences 45, no.3 (2015): 489 - 495.
MLA Belviranli Muaz,BÜYÜKBAŞ Sadık,GÖKBEL Hakkı,OKUDAN Nilsel Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences, vol.45, no.3, 2015, ss.489 - 495.
AMA Belviranli M,BÜYÜKBAŞ S,GÖKBEL H,OKUDAN N Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences. 2015; 45(3): 489 - 495.
Vancouver Belviranli M,BÜYÜKBAŞ S,GÖKBEL H,OKUDAN N Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences. 2015; 45(3): 489 - 495.
IEEE Belviranli M,BÜYÜKBAŞ S,GÖKBEL H,OKUDAN N "Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats." Turkish Journal of Medical Sciences, 45, ss.489 - 495, 2015.
ISNAD Belviranli, Muaz vd. "Effects of grape seed extract on oxidative stress and antioxidant defensemarkers in streptozotocin-induced diabetic rats". Turkish Journal of Medical Sciences 45/3 (2015), 489-495.
Sayfa Oluşturulma Tarihi
17-04-2024 2:55

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