Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study

Esme, Hıdır; CANBİLEN, Aydan; ÖZTÜRK, Kayhan; ÇALIK, Mustafa; Baran, Yusuf

Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study

Yusuf BARAN, (Konya Eğitim ve Araştırma Hastanesi,Kulak Burun Boğaz Kliniği, Konya, Türkiye)

Kayhan ÖZTÜRK, (Selçuk Üniversitesi, Selçuklu Tıp Fakültesi, Kulak Burun Boğaz Hastalıkları Anabilim Dalı, Konya, Türkiye)

Mustafa ÇALIK, (Konya Eğitim ve Araştırma Hastanesi, Göğüs Cerrahisi Anabilim Dalı, Konya, Türkiye)

Aydan CANBİLEN, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Konya, Türkiye)

Hıdır ESME (Konya Eğitim ve Araştırma Hastanesi, Göğüs Cerrahisi Anabilim Dalı, Konya, Türkiye)

Journal of Medical Updates

2 0

Yıl: 2013 Cilt: 3 Sayı: 3 Sayfa Aralığı: 101 - 108 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study

Öz:

Amaç: Larenks rekonstrüksiyonunda Dacron® implant kullanımını değerlendirmek ve bu materyalin baş-boyun cerrahilerinde potansiyel endikasyonlarını tartışmak.Yöntem: Her biri 12 Wistar albino sıçandan ibaret üç grup oluşturuldu. Grup 1 kontrol grubu olup bu gruptaki sıçanlarda krikoit kıkırdaktan 5. trakea halkasına kadar 6 mm uzunluğunda kesi yapılarak elde edilen 5¥5¥5 mmlik otolog kosta kıkırdağı transplantasyonu yoluyla anterior larengotrakeal rekonstrüksiyon gerçekleştirildi. Grup 2de, Grup 1de olduğu gibi trakea eşit uzunluklarda bölümlere ayrıldı ve aynı büyüklükte homolog kıkırdak nakliyle anterior larengotrakeal rekonstrüksiyon gerçekleştirildi. Grup 3de trakea, Grup 1e eşit bölümlere ayrıldı ve aynı büyüklükte bir Dacron® greftiyle rekonstrüksiyon yapıldı. İmplantasyondan sonraki 12. haftada sıçanlar sakrifiye edildi ve hasta gruplar trakea kesit alanı, parsiyel oksijen basıncı, epitelizasyon, neovaskülarizasyon, enflamatuar hücre infiltrasyonu ve yeni kondrosit oluşumu açısından karşılaştırıldı.Bulgular: Grup 1de postoperatif 2. günde pnömotoraks ve Grup 3de ise 8. günde granülasyon ve krutlanma nedeniyle birer deney hayvanı kaybedildi. Trakea enine kesit alanı ve parsiyel oksijen basıncı açısından gruplar arasında herhangi bir istatistiksel farklılık yoktu. Grup 2 ve Grup 3 arasında epitel gelişimi ve neovaskülarizasyon açısından istatistiksel bir farklılık yoktu ancak Grup 1, diğer iki gruptan anlamlı derecede daha iyi idi. Greft reddinde lenfosit infiltrasyonu önemli bir parametre olup gruplar arasında önemli bir farklılık vardı. Grup 1, diğer 2 gruptan daha iyi olduğu gibi Grup 3ün, Grup 2den hatırı sayılır derecede farklı olduğu belirlendi. Yeni kondrosit gelişimi açısından Grup 1. ile Grup 2 arasında anlamlı bir farklılık vardı.Sonuç: Epitel, neovaskülarizasyon ve kondrosit gelişim skorları daha iyi olduğu için otojen kosta kıkırdağı trakea ve larenks için ideal greft materyalidir. Dacron®un kullanıldığı grupta stenoz, enfeksiyon ve rejeksiyon olmadığı, tüm deney hayvanlarında epitel gelişimi ve neovaskülarizasyon tatminkâr derecede oluştuğu için larenks rekonstrüksiyonunda Dacron® implantları güvenle kullanılabilmektedir.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Larengotrakeal rekonstrüksiyonda kosta kıkırdağıyla Dacron® greftin karşılaştırması: Deneysel çalışma

Öz:

Objective: To evaluate the use of the Dacron® implant in laryngeal reconstruction and to discuss potential indications of this material in head and neck surgeries.Methods: Three groups consisting of 12 Wistar albino rats in each were formed, Group 1 was the control group and anterior laryngotratecheal reconstruction was performed by transplanting autologous costal cartilage sized 5¥5¥5 mm obtained by 6 mm longitunal sectioning extending from cricoid cartilage to the 5th tracheal ring. Group 2: Trachea sectioning was performed in equal lengths as in Group 1, and anterior laryngotracheal reconstruction was carried out by transplanting the same sized homologous cartilage. Group 3: Trachea sectioning was performed in equal lengths as in Group 1 and anterior laryngotracheal reconstruction was carried out by Dacron® graft in the same size. The rats were sacrificed in the 12th week of the implantation, and the groups were compared in terms of their tracheal section area, partial oxygen pressure, epithelialization, neovascularization, inflammatory cell infiltration and new chondrocyte development.Results: One experimental animal in Group 1 was lost due to pneumothorax on the second day postoperatively. In Group 3, one animal died because of development of granulation and crusting on the 8th day postoperatively. There was no statistically significant difference between the groups in terms of tracheal cross sectional area, and partial oxygen pressure. While there was no significant difference in terms of epithelial development and neovascularization between Groups 2 and 3, while Group 1 was significantly better than Groups 2 and 3. Lymphocytic infiltration is an essential parameter in graft rejection and there was a significant difference between the groups. Group 1 was better than the other two groups but it was determined that Group 3 was considerably different from Group 2. There was a significant difference between Groups 1 and 2 in terms of new chondrocyte development.Conclusion: Autogenous costal cartilage is an ideal graft material for trachea and larynx since scores of epithelial development, neovascularization and chondrocyte development are better. Dacron® implant can be used safely in laryngeal reconstrucion as stenosis, infection and rejection did not occur in Group 3 exposed to Dacron®, and epithelial development and nerovascularization occurred satisfactorily in all experimental animals.

Anahtar Kelime:

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Baran Y, ÖZTÜRK K, ÇALIK M, CANBİLEN A, Esme H (2013). Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. , 101 - 108.
Chicago	Baran Yusuf,ÖZTÜRK Kayhan,ÇALIK Mustafa,CANBİLEN Aydan,Esme Hıdır Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. (2013): 101 - 108.
MLA	Baran Yusuf,ÖZTÜRK Kayhan,ÇALIK Mustafa,CANBİLEN Aydan,Esme Hıdır Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. , 2013, ss.101 - 108.
AMA	Baran Y,ÖZTÜRK K,ÇALIK M,CANBİLEN A,Esme H Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. . 2013; 101 - 108.
Vancouver	Baran Y,ÖZTÜRK K,ÇALIK M,CANBİLEN A,Esme H Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. . 2013; 101 - 108.
IEEE	Baran Y,ÖZTÜRK K,ÇALIK M,CANBİLEN A,Esme H "Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study." , ss.101 - 108, 2013.
ISNAD	Baran, Yusuf vd. "Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study". (2013), 101-108.

APA	Baran Y, ÖZTÜRK K, ÇALIK M, CANBİLEN A, Esme H (2013). Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. Journal of Medical Updates, 3(3), 101 - 108.
Chicago	Baran Yusuf,ÖZTÜRK Kayhan,ÇALIK Mustafa,CANBİLEN Aydan,Esme Hıdır Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. Journal of Medical Updates 3, no.3 (2013): 101 - 108.
MLA	Baran Yusuf,ÖZTÜRK Kayhan,ÇALIK Mustafa,CANBİLEN Aydan,Esme Hıdır Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. Journal of Medical Updates, vol.3, no.3, 2013, ss.101 - 108.
AMA	Baran Y,ÖZTÜRK K,ÇALIK M,CANBİLEN A,Esme H Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. Journal of Medical Updates. 2013; 3(3): 101 - 108.
Vancouver	Baran Y,ÖZTÜRK K,ÇALIK M,CANBİLEN A,Esme H Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study. Journal of Medical Updates. 2013; 3(3): 101 - 108.
IEEE	Baran Y,ÖZTÜRK K,ÇALIK M,CANBİLEN A,Esme H "Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study." Journal of Medical Updates, 3, ss.101 - 108, 2013.
ISNAD	Baran, Yusuf vd. "Comparison of costal cartilage and Dacron® graft in laryngotracheal reconstruction: an experimental study". Journal of Medical Updates 3/3 (2013), 101-108.