The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

AKSOY, Hülya; AKÇAY, Fatih; TOKER, Aysun; Suleyman, Halis; erdem, havva; ÖZGEN, Ufuk

The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

Aysun TOKER, (Necmettin Erbakan Üniversitesi, Meram Tıp Fakültesi, Biyokimya Anabilim Dalı, Konya, Türkiye)

Fatih AKÇAY, (Atatürk Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)

Hülya AKSOY, (Atatürk Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Erzurum, Türkiye)

Halis SÜLEYMAN, (Atatürk Üniversitesi, Tıp Fakültesi, Farmakoloji Anabilim Dalı, Erzurum, Türkiye)

Ufuk ÖZGEN, (Karadeniz Teknik Üniversitesi, Eczacılık Fakültesi, Farmakognozi Anabilim Dalı, Trabzon, Türkiye)

Havva ERDEM (Dicle Üniversitesi, Eczacılık Fakültesi, Diyarbakır, Türkiye)

Turkish Journal of Medical Sciences

0 0

Yıl: 2013 Cilt: 43 Sayı: 2 Sayfa Aralığı: 315 - 320 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats

Öz:

Aim: We investigated the effects of acetyl shikonin isolated from Onosma armeniacum on ethanol-induced gastric injury (ulcer) and on oxidant antioxidant parameters in the gastric tissue of rats. Materials and methods: Eighteen albino Wistar male rats were divided into 3 different experimental groups. Group 1 (n = 6) was the control group, group 2 (n = 6) was the ethanol group, and group 3 (n = 6) was the acetyl shikonin + ethanol group. The animals in group 3 were given ethanol (1 mL, 50% v/v) 30 min after acetyl shikonin (40 mg/kg) administration by orogastric tube. Results: The lowest mean nitric oxide level; the lowest superoxide dismutase, glutathione peroxidase, and glutathione S-transferase activities; and the highest malondialdehyde level and myeloperoxidase activity were obtained in group 2. It was observed that acetyl shikonin increased the activities of antioxidant enzymes and the level of nitric oxide and decreased the level of malondialdehyde and the activity of myeloperoxidase in group 3. In histopathological evaluation, minimal lymphocyte infiltration and edema were seen but no ulcerations were observed in group 3. Conclusion: The data revealed that the antiulcer effect of acetyl shikonin might be due to an increase in antioxidant enzyme activity and nitric oxide levels, and a decrease in malondialdehyde levels and myeloperoxidase activity

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Mozsik G, Javor T. A biochemical and pharmacological approach to the genesis of ulcer disease. I. A model study of ethanol-induced injury to gastric mucosa in rats. Dig Dis Sci 1988; 33: 92105.
2. Kvietys PR, Twohig B, Danzell J, Specian RD. Ethanol-induced injury to the rat gastric mucosa. Role of neutrophil and xanthine oxidase-derived radicals. Gastroenterology 1990; 98: 90920.
3. Salim AS. Removing oxygen-derived free radicals stimulates healing of ethanol induced erosive gastritis in rat. Digestion 1990; 47: 248.
4. Giordano OS, Guerreiro E, Pestchanker MJ, Guzman J, Pastor D, Guardia T. The gastric cytoprotective effect of several sesquiterpene lactones. J Nat Prod 1990; 53: 8039.
5. Szabo S, Trier JS, Brown A, Schnoor J. Early vascular injury and increased vascular permeability in gastric mucosal injury caused by ethanol in the rat. Gastroenterology 1985; 88: 228 36.
6. Kato S, Kitanura M, Korolkiewicz RP, Takeuchi K. Role of nitric oxide in regulation of gastric acid secretion in rats: effects of NO donors and NO synthase inhibitor. Br J Pharmacol 1998; 123: 83946.
7. Szabo S, Trier JS, Frankel PW. Sulfhydryl compounds may mediate gastric cytoprotection. Science 1981; 214: 2002.
8. Holzer P, Livingston EH, Saria A, Guth PH. Sensory neurons mediate protective vasodilatation in rat gastric mucosa. Am J Physiol 1991; 260: 36370.
9. Szelenyi I, Brune K. Possible role of oxygen free radicals in ethanol-induced gastric mucosal damage in rats. Dig Dis Sci 1988; 33: 86571.
10. Hirokawa M, Miura S, Yoshida H, Kurose I, Shigematsu T, Hokari R et al. Oxidative stress and mitochondrial damage precedes gastric mucosal cell death induced by ethanol administration. Alcohol Clin Exp Res 1998; 22: 111S4S.
11. La Casa C, Villegas I, Alarcon de la Lastra C, Motilva V, Martin Calero MJ. Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol induced gastric lesions. J Ethnopharmacol 2000; 71: 4553.
12. Papageorgiou VP. 1 H-NMR spectra of naturally occurring isohexenylnaphthazarin pigments. Planta Medica 1979; 37: 1857.
13. Brigham LA, Michaels PJ, Flores HE. Cell-specific production and antimicrobial activity of naphthoquinones in roots of lithospermum erythrorhizon. Plant Physiol 1999; 119: 41728.
14. Riedl H. Onosma L. In: Davis PH, editor. Flora of Turkey and the East Aegean Islands, Vol 6. Edinburgh: Edinburgh University Press; 1978. p.3423.
15. Özgen U, Coşkun M. Ilıca (Erzurum) İlçesine Bağlı Köylerde Halk İlacı Olarak Kullanılan Bitkiler. XIII. Bitkisel İlaç Hammaddeleri Toplantısı, İstanbul; 2000.
16. Özgen U, Houghton PJ, Ogundipe Y, Coşkun M. Antioxidant and antimicrobial activities of Onosma argentatum and Rubia peregrina. Fitoterapia 2003; 74: 6825.
17. Sezik E, Yeşilada E, Tabata M, Honda G, Takashi Y, Fujita T et al. Traditional medicine in Turkey. VIII. Folk medicine in east Anatolia; Erzurum, Erzincan, Ağri, Kars, Iğdir Provinces. Econ Bot 1997; 51: 195211.
18. Cadirci E, Suleyman H, Aksoy H, Halici Z, Ozgen U, Koc A et al. Effects of Onosma armeniacum root extract on ethanolinduced oxidative stress in stomach tissue of rats. Chem Biol Interact 2007; 170: 408.
19. Sekine T, Masumizu T, Maitani Y, Nagai T. Evaluation of superoxide anion radical scavenging activity of shikonin by electron spin resonance. Int J Pharm 1998; 174: 1339.
20. Habig WH, Pabst MJ, Jakoby WB. Glutathione S-transferases. The first enzymatic step in mercapturic acid formation. J Biol Chemistry 1974; 249: 71309.
21. Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70: 15869.
22. Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clin Chem 1988; 34: 497500.
23. Wei H, Frenkel K. In vivo formation of oxidized DNA bases in tumor promoter-treated mouse skin. Can Res 1991; 51: 44439.
24. Okhawa H, Ohishi N, Yagi K. Assay for lipid peroxidase in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979; 95: 3518.
25. Moshage H, Kok B, Huizenga JR, Jansen PLM. Nitrite and nitrate determination in plasma: a critical evaluation. Clin Chem 1995; 41: 8926.
26. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 24854.
27. Papageorgiou VP, Assimopoulou AN, Couladouros EA, Hepworth D, Nicolaou KC. The chemistry and biology of alkannin, shikonin and related naphthazarin natural products. Angew Chem Int Ed 1999; 38: 270301.
28. Hayashi M. Pharmacological studies of Shikon and Tooki. 1. Pharmacological effects of the water and ether extracts. Nihon Yakurigaku Zasshi 1977; 73: 17791.
29. Glavin GB, Szabo S. Experimental gastric mucosal injury: laboratory models reveal mechanisms of pathogenesis and new therapeutic strategies. FASEB J 1992; 6: 82531.
30. Kurban S, Mehmetoğlu İ. The effect of alcohol on total antioxidant activity and nitric oxide levels in the sera and brains of rats. Turk J Med Sci 2008; 38: 199204.
31. Öztürk F, Vardı N, Cengiz N, Eşrefoğlu M, Otlu A. Microscopy of rat thoracic aorta in chronic ethanol ıntake. Turk J Med Sci 2002; 32: 11720.
32. Suzuki Y, Ishihara M, Segami T, Ito M. Anti-ulcer effects of antioxidants, quercetin, alpha-tocopherol, nifedipine and tetracycline in rats. Jpn J Pharmacol 1998; 78: 43541.
33. Bilici D, Süleyman H, Banoğlu ZN, Kiziltunç A, Avci B, Çiftçioğlu A et al. Melatonin prevents ethanol-induced gastric mucosal damage possibly due to its antioxidant effect. Dig Dis Sci 2002; 47: 85661.
34. Ito M, Suzuki Y, Ishihara M, Suzuki Y. Anti-ulcer effects of antioxidants: effect of probucol. Eur J Pharmacol 1998; 354: 18996.
35. Zimmerman BJ, Granger D. Oxygen free radicals and the gastrointestinal tract: role of ischemia-reperfusion injury. Hepatogastroenterology 1994; 41: 33742.
36. Nakatami N. Phenolic antioxidants from herbs and spices. Biofactors 2000; 13: 1416.
37. Hamaishi K, Kojima R, Ito M. Anti-ulcer effect of tea catechin in rats. Biol Pharm Bull 2006; 29: 220613.
38. Zamora Rodriguez ZB, Gonzalez Alvarez R, Guanche D, Merino N, Hernandez Rosales F, Menendez Cepero S et al. Antioxidant mechanism is involved in the gastroprotective effects of ozonized sunflower oil in ethanol-induced ulcers in rats. Mediators Inflamm 2007; 2007: 65873.
39. Chandranath SI, Bastaki SM, Singh J. A comparative study on the activity of lansoprazole, omeprazole and PD-136450 on acidified ethanol- and indomethacin-induced gastric lesions in the rat. Clin Exp Pharmacol Physiol 2002; 29: 17380.
40. Gaboury J, Woodman RC, Granger DN, Reinhardt P, Kubes P. Nitric oxide leukocyte adherence: role of superoxide. Am J Physiol 1993; 265: 8627.

APA	TOKER A, AKÇAY F, AKSOY H, Suleyman H, ÖZGEN U, erdem h (2013). The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. , 315 - 320.
Chicago	TOKER Aysun,AKÇAY Fatih,AKSOY Hülya,Suleyman Halis,ÖZGEN Ufuk,erdem havva The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. (2013): 315 - 320.
MLA	TOKER Aysun,AKÇAY Fatih,AKSOY Hülya,Suleyman Halis,ÖZGEN Ufuk,erdem havva The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. , 2013, ss.315 - 320.
AMA	TOKER A,AKÇAY F,AKSOY H,Suleyman H,ÖZGEN U,erdem h The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. . 2013; 315 - 320.
Vancouver	TOKER A,AKÇAY F,AKSOY H,Suleyman H,ÖZGEN U,erdem h The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. . 2013; 315 - 320.
IEEE	TOKER A,AKÇAY F,AKSOY H,Suleyman H,ÖZGEN U,erdem h "The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats." , ss.315 - 320, 2013.
ISNAD	TOKER, Aysun vd. "The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats". (2013), 315-320.

APA	TOKER A, AKÇAY F, AKSOY H, Suleyman H, ÖZGEN U, erdem h (2013). The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. Turkish Journal of Medical Sciences, 43(2), 315 - 320.
Chicago	TOKER Aysun,AKÇAY Fatih,AKSOY Hülya,Suleyman Halis,ÖZGEN Ufuk,erdem havva The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. Turkish Journal of Medical Sciences 43, no.2 (2013): 315 - 320.
MLA	TOKER Aysun,AKÇAY Fatih,AKSOY Hülya,Suleyman Halis,ÖZGEN Ufuk,erdem havva The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. Turkish Journal of Medical Sciences, vol.43, no.2, 2013, ss.315 - 320.
AMA	TOKER A,AKÇAY F,AKSOY H,Suleyman H,ÖZGEN U,erdem h The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. Turkish Journal of Medical Sciences. 2013; 43(2): 315 - 320.
Vancouver	TOKER A,AKÇAY F,AKSOY H,Suleyman H,ÖZGEN U,erdem h The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats. Turkish Journal of Medical Sciences. 2013; 43(2): 315 - 320.
IEEE	TOKER A,AKÇAY F,AKSOY H,Suleyman H,ÖZGEN U,erdem h "The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats." Turkish Journal of Medical Sciences, 43, ss.315 - 320, 2013.
ISNAD	TOKER, Aysun vd. "The effects of acetyl shikonin isolated from Onosma armeniacum on oxidative stress in ethanol-induced ulcer model of rats". Turkish Journal of Medical Sciences 43/2 (2013), 315-320.