Aim: This study was conducted to assess the effect of the guide nursing course on the opinions of nurses about guide nursing. Materials and Methods: The sample of the study was 221 nurses who worked in a university hospital and agreed to participate in the research. Data from the pre-test and post-test single-group semi-experimental study were collected before and after the guide nursing course using the questionnaire form between 06-08 February 2017. The number, percentage, mean and standard deviation of the study were analyzed with McNemar tests. Results: When the opinions of the nurses on the duties of the guide nurse were examined, it was stated that "undertaking the responsibility of the educator in practice" (p<0.001) and "providing necessary evidences to evaluate the student's success" (p<0.001) opinions were found to change significantly in the positive direction after the training compared to before the training. When the opinions of the nurses on the role behaviors of the guide nurse were examined, it was determined that "addressing students by name" (p<0.001), "no repressive attitudes when mentoring" (p<0.001), "interviewing with the student about how to improve performance (p<0.001), "critical thinking and helping to investigate the subjects in depth" (p<0.001) opinions were found to change significantly in the positive direction after the training compared to before the training. Conclusion: These findings showed that the given courses have a positive influence on the opinions of nurses about guide nursing.