Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey
Yıl: 2018 Cilt: 24 Sayı: 1 Sayfa Aralığı: 12 - 17 Metin Dili: İngilizce DOI: 10.4274/vhd.0013 İndeks Tarihi: 05-02-2019
Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey
Öz: Objectives: Hepatitis C virus (HCV) is an important viral agent ofhepatitis, cirrhosis and hepatocellular carcinoma. In our study, weaimed to determine the HCV genotype distribution among patientswith HCV who presented to our hospital in a city in the CentralAnatolia Region of Turkey.Materials and Methods: Results of 480 patients who were positivefor HCV RNA and whose serum samples were sent to our laboratoryfrom various inpatient and outpatient clinics of the hospital with apre-diagnosis of hepatitis C between January 2010 and May 2017were retrospectively screened. In HCV genotype determination,a commercially available kit (Ampliquality HCV-TS, AB Analitica®,Italy) based on Reverse Line Blot was used in accordance with themanufacturer’s recommendations. Genotype distributions wereanalyzed by years and by age. The first and only one test results ofthe same patients were evaluated.Results: Of the patients whose genotyping was made, 260(54.2%) were female and 220 (45.8%) were male. It was found that396 (82.6%) of 480 patients were with genotype 1b, 17 (3.5%) -genotype 1a, 15 (3.1%) - genotype 3a, 14 (2.9%) - genotype 1, 9(1.9%) - genotype 4, 8 (1.7%) - genotype 2, 6 (1.3%) - genotype2b, 5 (1.0%) - genotype 1a/1b, 4 (0.8%) - genotype 2a/2c, 3 (0.6%)- genotype 4a, 1 (0.2%) - genotype 3, 1 (0.2%) - genotype 5a and 1(0.2%) patient was with genotype 6.Conclusion: In chronic HCV patients admitted to our hospital,genotype 1b, which had the highest prevalence in our country, wasdetected with a rate of 82.6%. In addition, the presence of raregenotypes 5a and 6 in our country has been shown.
Anahtar Kelime: Konular:
ç Anadolu Bölgesinde Bir Şehirde Hepatit C Hastalarının Yedi Yıllık Genotip Dağılımları
Öz: Amaç: Hepatit C virüsü (HCV), hepatit, siroz ve hepatosellüler karsinomun önemli bir viral etkenidir. Çalışmamızda, İç Anadolu Bölgesi’nde bir şehirde, hastanemize başvuran HCV ile enfekte hastaların HCV genotip dağılımlarını saptamayı hedefledik. Gereç ve Yöntemler: Ocak 2010-Mayıs 2017 tarihleri arasında, laboratuvarımıza hastanenin çeşitli klinik ve polikliniklerinden hepatit C ön tanısı ile serum örneği gönderilen ve HCV RNA pozitifliği olan 480 hastanın sonuçları retrospektif olarak tarandı. HCV genotip tayininde, ters hibridizasyon (Reverse Line Blot) temeline dayanan ticari bir kit (Ampliquality HCV-TS; AB Analitica®, İtalya), üretici firma önerileri doğrultusunda kullanıldı. Genotip dağılımları yıllara ve yaşlara göre incelendi. Aynı hastaların ilk ve tek test sonucu değerlendirmeye alındı. Bulgular: Genotiplemesi yapılan hastaların 260’ı (%54,2) kadın, 220’si (%45,8) erkek idi. Toplam 480 hastanın 396’sı (%82,6) genotip 1b, 17’si (%3,5) genotip 1a, 15’i (%3,1) genotip 3a, 14’ü (%2,9) genotip 1, 9’u (%1,9) genotip 4, 8’i (%1,7) genotip 2, 6’sı (%1,3) genotip 2b, 5’i (%1,0) genotip 1a/1b, 4’ü (%0,8) genotip 2a/2c, 3’ü (%0,6) genotip 4a, 1’i (%0,2) de genotip 3, genotip 5a ve genotip 6 olarak bulunmuştur. Sonuç: Hastanemize başvuran kronik HCV hastalarında en sık; ülkemizde de en yüksek prevalansa sahip olan: genotip 1b, %82,6 oranıyla saptanmıştır. Ayrıca, ülkemizde az görülen genotip 5a ve genotip 6’nın varlığı gösterilmiştir.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Aman W, Mousa S, Shiha G, Mousa SA. Current status and future directions in the management of chronic hepatitis C. Virol J. 2012;9:57.
- Lindenbach BD, Rice CM. Unravelling hepatitis C virus replication from genome to function. Nature. 2005;18;436:933-938.
- Simmonds P, Bukh J, Combet C, Deleage G, Enomoto N, Feinstone S, Halfon P, Inchauspé G, Kuiken C, Maertens G, Mizokami M, Murphy DG, Okamoto H, Pawlotsky JM, Penin F, Sablon E, Shin-I T, Stuyver LJ, Thiel HJ, Viazov S, Weiner AJ, Widell A. Consensus proposals for a unified system of nomenclature of hepatitis C virus genotypes. Hepatology. 2005;42:962-973.
- Smith DB, Bukh J, Kuiken C, Muerhoff AS, Rice CM, Stapelton JT, Simmonds P. Expanded classification of hepatitis C virus into 7 genotypes and 67 subtypes: updated criteria and genotype assignment web resource. Hepatology. 2014;59:318-327.
- Jimenez-Mendez R, Uribe-Salas F, López-Guillen P, Cisneros-Garza L, Castañeda-Hernandez G. Distribution of HCV genotypes and HCV RNA viral load in different regions of Mexico. Ann Hepatol. 2010;9:33-39.
- Zein NN. Clinical significance of hepatitis C virus genotypes. Clin Microbiol Rev. 2000;13:223-235.
- Ohno O, Mizokami M, Wu RR, Saleh MG, Ohba K, Orito E, Mukaide M, Williams R, Lau JY. New hepatitis C virus (HCV) genotyping system that allows for identification of HCV genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a. J Clin Microbiol. 1997;35:201-207.
- McOmish F, Yap PL, Dow BC, Follett EA, Seed C, Keller AJ, Cobain TJ, Krusius T, Kolho E, Naukkarinen R, et al. Geographical distribution of hepatitis C virus genotypes in blood donors: an international collaborative survey. J Clin Microbiol. 1994;32:884- 892.
- Simmonds P, Holmes EC, Cha TA, Chan SW, McOmish F, Irvine B, Beall E, Yap PL, Kolberg J, Urdea MS. Classification of hepatitis C virus into six major genotypes and a series of subtypes by phylogenetic analysis of the NS-5 region. J Gen Virol. 1993;74:2391-2399.
- Zarkesh-Esfahani SH, Kardi MT, Edalati M. Hepatitis C virus genotype frequency in Isfahan province of Iran: a descriptive cross-sectional study. Virol J. 2010;7:69.
- Tezcan S, Ulger M, Aslan G, Yaraş S, Altıntas E, Sezgin O, Emekdaş G, Gürer Giray B, Sungur MA. Determination of Hepatitis C Virus Genotype Distribution in Mersin Province, Turkey. Mikrobiyol Bul. 2013;47:332-338.
- Altuglu I, Sertöz R, Aksoy A, Gürsel D, Tüzüner U, Günşar F. Possible transmission risks and genotype distribution of hepatitis C virus infection in Western Turkey. Turk J Gastroenterol. 2013;24:349-355.
- Altindis M, Dal T, Akyar I, Karatuna O, Gokahmetoglu S, Tezcan Ulger S, Kulah C, Uzun B, Şener AG, Ozdemir M, Aydogan S, Kuskucu MA, Midilli K, Otlu B, Celen MK, Buruk K, Guducuoglu H. Six-year distribution pattern of hepatitis C virus in Turkey: a multicentre study. Biotechnology & Biotechnological Equipment. 2016;30:335-340.
- Oral Zeytinli U, Muhterem Yucel F, Daldaban Dincer S, Yanilmaz O, Aksaray S, Ozdil K. Distribution of Hepatitis C Virus Genotypes in the Region of ‘Istanbul Northern Anatolian Association of Public Hospitals’. Viral Hepat J. 2017;23:10-13.
- Sağlik İ, Mutlu D, Öngut G, İnan D, Öğünç D, Can Sarinoğlu R, Özhak Baysan B, Gültekin M, Çolak D. Distribution of Hepatitis C Virus Genotypes among Patients with Chronic Hepatitis C Infection in Akdeniz University Hospital, Antalya, Turkey: A FiveYear Evaluation. Mikrobiyol Bul. 2014;48:429-437.
- Kayman T, Karakukcu C, Karaman A, Gozutok F. Genotypic Distribution of Hepatitis C Virus Infection in Kayseri Region. Journal of Turkish Microbiological Society. 2012;42:21-26.
- Kuscu F, Komur S, Inal AS, Cengaver Ulu A, Kurtaran B, Taşova Y, Unlu B, Mıdıklı D, Dogan Tombul Z, Yilmaz G, Suntun BM, Aksu HSZ. Changing Epidemiology of Chronic Hepatitis C in Adana. Viral Hepat J. 2014;20:15-18.
- Oztürk AB, Doğan UB, Oztürk NA, Ozyazici G, Demir M, Akin MS, Böngöl AS. Hepatitis C virus genotypes in Adana and Antakya regions of Turkey. Turk J Med Sci. 2014;44:661-665.
- Agca H, Kazak E, Mıstık R. Distribution of Hepatitis C Virus Genotypes in the South Marmara Region. J Clin Anal Med. 2015;6:190-192.
- Celik C, Bakici MZ, Kaygusuz R, Ertürk R. The Searching of HCV Genotyping Distributions in the Region of Sivas. Viral Hepat J. 2010;16:106-110.
- Borcak D, Çagır U, Yalciner A. Distribution of Hepatitis C Virus Genotypes and Their Association with Serum Alanine Aminotransferases and Quantitative Serum HCV RNA Levels. Journal of Antimicrobial Chemotherapy. 2015;29:36-40.
- Çekın Y, Gür N, Çekın AH, Altuğlu İ, Yazan Sertöz R. Investigation of Hepatitis C Virus Genotype Distribution in Patients with Chronic Hepatitis C Infections in Antalya Training and Research Hospital, Turkey. Mikrobiyol Bul. 2014;48:484-490.
- Gökahmetoğlu S, Atalay MA, Kılınç A. Determination of the Hepatitis C Virus Genotypes With. Pyrosequencing Method. Erciyes Med J. 2011;33:99-102.
- Aktas O, Ozbek A, Aydın H, Ozkulekci MB. Distribution of HCV genotypes in patients of with chronic hepatitis C in the eastern anatolia region. Viral Hepat J. 2014;20:91-94.
- Kirisci O, Calıskan A, Kocturk S, Erdogmus P, Gul M. The Relationship Between Distribution of HCV-RNA and ALT-AST Levels with Genotypes of Hepatitis C Virus Infected Patients. Viral Hepat J. 2013;19:67-70.
- Sanlidag T, Akçali S, Ozbakkaloglu B, Ertekin D, Akduman E. Distribution of hepatitis C virus genotypes in Manisa region, Turkey. Mikrobiyol Bul. 2009;43:613-618.
- Ozbek E, Ozekinci T, Mese S, Atmaca S. Hepatitis C virus genotypes are changing in the Southeast of Turkey. Biotechnol Biotechnol Equipment. 2009;23:1521-1523.
- Akgun S, Tarhan G, Sayiner HS, Akgun I, Kok S. Determination of hepatitis C virus genotype distribution in Adıyaman province. Ortadoğu Medical Journal. 2017;9:1-5.
- Demircili ME, Ozdemir M, Feyzioglu B, Baysal B. The Efficiency of Hepatitis C Virus Core Antigen Test in the Diagnosis of Hepatitis C Infection. Viral Hepat J. 2016;22:18-22.
- Karslıgil T, Savas E, Savas MC. Genotype distribution and 5’UTR nucleotide changes in hepatitis C virus. Balkan Med J. 2011;28:232-236.
- Özen Karataylı SC, Bozdayı AM. Hepatitis C Virus Virology, Genotypes and Subgenotypes, Diagnosis. Türkiye Klinikleri J Gastroenterohepatol-Special Topics. 2010;3:70-76.
- Medhat A, Shehata M, Magder LS, Mikhail N, Abdel-Baki L, Nafeh M, Abdel-Hamid M, Strickland GT, Fix AD. Hepatitis c in a community in Upper Egypt: risk factors for infection. Am J Trop Med Hyg. 2002;66:633-638.
APA | TÜZÜNER U, GÜLCEN B, OZDEMIR M, Feyzioğlu B, BAYKAN M (2018). Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. , 12 - 17. 10.4274/vhd.0013 |
Chicago | TÜZÜNER Uğur,GÜLCEN Begüm Saran,OZDEMIR MEHMET,Feyzioğlu Bahadır,BAYKAN Mahmut Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. (2018): 12 - 17. 10.4274/vhd.0013 |
MLA | TÜZÜNER Uğur,GÜLCEN Begüm Saran,OZDEMIR MEHMET,Feyzioğlu Bahadır,BAYKAN Mahmut Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. , 2018, ss.12 - 17. 10.4274/vhd.0013 |
AMA | TÜZÜNER U,GÜLCEN B,OZDEMIR M,Feyzioğlu B,BAYKAN M Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. . 2018; 12 - 17. 10.4274/vhd.0013 |
Vancouver | TÜZÜNER U,GÜLCEN B,OZDEMIR M,Feyzioğlu B,BAYKAN M Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. . 2018; 12 - 17. 10.4274/vhd.0013 |
IEEE | TÜZÜNER U,GÜLCEN B,OZDEMIR M,Feyzioğlu B,BAYKAN M "Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey." , ss.12 - 17, 2018. 10.4274/vhd.0013 |
ISNAD | TÜZÜNER, Uğur vd. "Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey". (2018), 12-17. https://doi.org/10.4274/vhd.0013 |
APA | TÜZÜNER U, GÜLCEN B, OZDEMIR M, Feyzioğlu B, BAYKAN M (2018). Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. Viral Hepatitis Journal, 24(1), 12 - 17. 10.4274/vhd.0013 |
Chicago | TÜZÜNER Uğur,GÜLCEN Begüm Saran,OZDEMIR MEHMET,Feyzioğlu Bahadır,BAYKAN Mahmut Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. Viral Hepatitis Journal 24, no.1 (2018): 12 - 17. 10.4274/vhd.0013 |
MLA | TÜZÜNER Uğur,GÜLCEN Begüm Saran,OZDEMIR MEHMET,Feyzioğlu Bahadır,BAYKAN Mahmut Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. Viral Hepatitis Journal, vol.24, no.1, 2018, ss.12 - 17. 10.4274/vhd.0013 |
AMA | TÜZÜNER U,GÜLCEN B,OZDEMIR M,Feyzioğlu B,BAYKAN M Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. Viral Hepatitis Journal. 2018; 24(1): 12 - 17. 10.4274/vhd.0013 |
Vancouver | TÜZÜNER U,GÜLCEN B,OZDEMIR M,Feyzioğlu B,BAYKAN M Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey. Viral Hepatitis Journal. 2018; 24(1): 12 - 17. 10.4274/vhd.0013 |
IEEE | TÜZÜNER U,GÜLCEN B,OZDEMIR M,Feyzioğlu B,BAYKAN M "Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey." Viral Hepatitis Journal, 24, ss.12 - 17, 2018. 10.4274/vhd.0013 |
ISNAD | TÜZÜNER, Uğur vd. "Seven-year Genotype Distribution Among Hepatitis C Patients in a City in the Central Anatolia Region of Turkey". Viral Hepatitis Journal 24/1 (2018), 12-17. https://doi.org/10.4274/vhd.0013 |