Carrier free cross-linked peroxidase aggregates: synthesis and characterization
Yıl: 2008 Cilt: 36 Sayı: 3 Sayfa Aralığı: 255 - 261 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022
Carrier free cross-linked peroxidase aggregates: synthesis and characterization
Öz: In this study, cross-linked Horseradish Peroxidase (HRP) aggregates were prepared. Bovin Serum Albumin (BSA), a proteic feeder, and glutaraldehyde, a bifunctional reactive, a crosslinker agent has also been used. The initial enzyme concentration (0.05 mg/ml), BSA (5 mg) and glutaraldehyde (GA) (4 μl/ml) amounts were optimized and maximum immobilized activities were obtained. The kinetic parameters of free and immobilised enzyme have been calculated by using Lineweaver-Burk plots. The saturation constant (Km) and the maximum reaction rate (Vm) of free enzyme were found as 2.33 mM and 0.670mmol/l.sec, respectively. The apparent kinetic parameters ($Km _{app}$ and $Vm _{app}$) of cross linked enzyme aggregates have been calculated as 33.4 mM and 0.127 mmol/l.sec, respectively.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- 1. K. Mosbach, Immobilized enzymes and cells (Part B) Methods Enzymology, Vol. 135, 1987.
- 2. W. Hartmeier, Immobilized Biocatalysts, Springer-Verlag, 1988.
- 3. D.S. Clarc, Can immobilization be exploited to modify enzyme activity? Trends Biotechnology, 12: 439-443, 1994.
- 4. J.M.S. Cabral, J.F. Kennedy, Immobilization techniques for altering thermal stability of enzymes, Thermo stability of Enzymes, edited by Gupta M. N. Berlin, Springer-Verlag , 163- 179, 1993.
- 5. S. Rocchietti, A.S.V. Urritia, M. Pregnolata, A. Tagliani, J.M. Guisan, R. Fernandez-Lafuente, M. Terreni, Influence of the enzyme derivative preparation and substrate structure on the enantioselective of penicillin G acylase, Enzyme Microb. Technol, 31:88-93, 2002.
- 6. L. Cao, L.V. Langen, R.A. Sheldon, Immobilised enzymes: carrier-bound or carrier –free, Current opinion in Biotechnology, 14: 387-394, 2003.
- 7. N.L. St Clair, M.A. Navia, Cross-linked enzyme crystals as robust biocatalysts, J. Am. Chem. Soc., 114: 7314-7316, 1992.
- 8. L. Cao, Immobilised enzymes: science or art?, Current opinion in Chemical Biology, 9: 217- 226, 2005.
- 9. J. Chen, J. Zhang, B. Han, Z. Li, J. Li, X. Feng, Synthesis of cross-linked aggregates (CLEAs) in CO2-expanded micellar solutions, Colloids and Surfaces B : Biointerfaces, 48: 72-76, 2006.
- 10. S.S. Caramori, K.F. Fernandes, Covalent immobilization of horseradish peroxidase onto poly(ethylene terephalate) – poly(aniline) composite, Process Biochemistry, 39: 883-888, 2004.
- 11. S. Shah, A. Sharma, M.N. Gupta, Preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder, Analytical Biochemistry, 351: 207-213, 2006.
- 12. R. Tyagi, R. Batra, M.N. Gupta, Amorphous enzyme aggregates: Stability towards heat and aqueous-organic co solvents mixtures, Enzyme and Microbial Technology, 24 : 348- 354, 1999.
- 13. B. Halphin, R. Pressey, J. Jen, N.J. Mondy, Purification and characterization of peroxidase isoenzyme from green peas ( Pisum sativum ), J.Food Sci., 54 : 44-49, 1989.
- 14. F. Rothstein, Differential precipitation of proteins: science and technology, In Protein Purification Process Engineering, Edited by Harrison R. G. New York: Marcel Decker, Inc, 115-208, 1994.
- 15. G.H. Broun, Chemically aggregated enzymes, in; Mosbach K. (Ed.), Methods in enzymology, Academic Press, New York, 263-269, 1997.
- 16. C. Topcular, H. Ayhan, Immobilisation of horseradish peroxidase onto monodisperse poly(glycidyl methacrylate) microspheres, J. Biomater. Sci. Polymer Edn. 18; 5; 595-607, 2007.
| APA | TOPÇULAR C, AYHAN H (2008). Carrier free cross-linked peroxidase aggregates: synthesis and characterization. , 255 - 261. |
| Chicago | TOPÇULAR Ceren,AYHAN HAKAN Carrier free cross-linked peroxidase aggregates: synthesis and characterization. (2008): 255 - 261. |
| MLA | TOPÇULAR Ceren,AYHAN HAKAN Carrier free cross-linked peroxidase aggregates: synthesis and characterization. , 2008, ss.255 - 261. |
| AMA | TOPÇULAR C,AYHAN H Carrier free cross-linked peroxidase aggregates: synthesis and characterization. . 2008; 255 - 261. |
| Vancouver | TOPÇULAR C,AYHAN H Carrier free cross-linked peroxidase aggregates: synthesis and characterization. . 2008; 255 - 261. |
| IEEE | TOPÇULAR C,AYHAN H "Carrier free cross-linked peroxidase aggregates: synthesis and characterization." , ss.255 - 261, 2008. |
| ISNAD | TOPÇULAR, Ceren - AYHAN, HAKAN. "Carrier free cross-linked peroxidase aggregates: synthesis and characterization". (2008), 255-261. |
| APA | TOPÇULAR C, AYHAN H (2008). Carrier free cross-linked peroxidase aggregates: synthesis and characterization. Hacettepe Journal of Biology and Chemistry, 36(3), 255 - 261. |
| Chicago | TOPÇULAR Ceren,AYHAN HAKAN Carrier free cross-linked peroxidase aggregates: synthesis and characterization. Hacettepe Journal of Biology and Chemistry 36, no.3 (2008): 255 - 261. |
| MLA | TOPÇULAR Ceren,AYHAN HAKAN Carrier free cross-linked peroxidase aggregates: synthesis and characterization. Hacettepe Journal of Biology and Chemistry, vol.36, no.3, 2008, ss.255 - 261. |
| AMA | TOPÇULAR C,AYHAN H Carrier free cross-linked peroxidase aggregates: synthesis and characterization. Hacettepe Journal of Biology and Chemistry. 2008; 36(3): 255 - 261. |
| Vancouver | TOPÇULAR C,AYHAN H Carrier free cross-linked peroxidase aggregates: synthesis and characterization. Hacettepe Journal of Biology and Chemistry. 2008; 36(3): 255 - 261. |
| IEEE | TOPÇULAR C,AYHAN H "Carrier free cross-linked peroxidase aggregates: synthesis and characterization." Hacettepe Journal of Biology and Chemistry, 36, ss.255 - 261, 2008. |
| ISNAD | TOPÇULAR, Ceren - AYHAN, HAKAN. "Carrier free cross-linked peroxidase aggregates: synthesis and characterization". Hacettepe Journal of Biology and Chemistry 36/3 (2008), 255-261. |
