Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province

Mehlika ALPER, (Muğla Sıtkı Koçman Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Hatice GÜNEŞ, (Muğla Sıtkı Koçman Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Arzu TATLIPINAR, (Muğla Sıtkı Koçman Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Bekir ÇÖL, (Muğla Sıtkı Koçman Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Hasan Sungur CİVELEK, (Muğla Sıtkı Koçman Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Cem ÖZKAN, (Ankara Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Dışkapı, Ankara)
Burcu POYRAZ (Muğla Sıtkı Koçman Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Turkish Journal of Agriculture and Forestry
1 0
Yıl: 2014 Cilt: 38 Sayı: 6 Sayfa Aralığı: 898 - 907 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province

Öz:
Bacillus thuringiensis (Bt) has a signifcant impact on biological pest control because of the insecticidal activity through itsparasporal inclusion bodies (crystal proteins). Fig is an economically important plant in Turkey; agricultural pests result in a considerableeconomic loss in fg quality and cultivation. Te aim of this work was to isolate, characterize, and determine the lepidopteran toxicityof Bt obtained from fg groves in Aydın Province. A total of 606 colonies (out of 1167) obtained from 380 samples were identifed as Btbased on parasporal crystal formation. Te highest Bt index of 0.60 was observed in the Kuyucak region. A total of 288 Bt isolates werecharacterized in terms of cry gene content by PCR analysis. It was found that the cry1 plus cry2 genotype was the most abundant (40%)in our collection. Bioactivity tests indicated that 6 isolates exhibited high mortalities against Cadra cautella and 3 isolates were foundto exhibit high toxicity against Carpophilus hemipterus. Moreover, 13 Bt isolates exhibiting toxic activity against fg pests were furthercharacterized based on specifc cry gene content, protein profles, and PCR-RFLP analysis. Among c ry1 genes, the cry1Aa , cry1Ab ,cry1Ac , cry1B, cry1C, cry1D , and cry1Ea genes were the most frequent (100%). Protein profles of isolates toxic to C. cautella werediferent from those of isolates toxic to C. hemipterus. PCR-RFLP analysis indicated that toxic isolates difered from the reference strainwith respect to cry1 type gene. Finally, it was concluded that Bt strains isolated from fg groves showed high level of toxicity against fgpests. Tese strains can serve as potential biopesticides for the control of C. cautella in the region as well as alternative biopesticides inthe case of pesticide resistance in insects.
Anahtar Kelime:

Konular: Orman Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ALPER M, güneş h, Tatlıpınar A, ÇÖL B, CİVELEK H, ÖZKAN C, POYRAZ B (2014). Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. , 898 - 907.
Chicago ALPER MEHLİKA,güneş hatice,Tatlıpınar Arzu,ÇÖL Bekir,CİVELEK HASAN SUNGUR,ÖZKAN Cem,POYRAZ Burcu Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. (2014): 898 - 907.
MLA ALPER MEHLİKA,güneş hatice,Tatlıpınar Arzu,ÇÖL Bekir,CİVELEK HASAN SUNGUR,ÖZKAN Cem,POYRAZ Burcu Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. , 2014, ss.898 - 907.
AMA ALPER M,güneş h,Tatlıpınar A,ÇÖL B,CİVELEK H,ÖZKAN C,POYRAZ B Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. . 2014; 898 - 907.
Vancouver ALPER M,güneş h,Tatlıpınar A,ÇÖL B,CİVELEK H,ÖZKAN C,POYRAZ B Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. . 2014; 898 - 907.
IEEE ALPER M,güneş h,Tatlıpınar A,ÇÖL B,CİVELEK H,ÖZKAN C,POYRAZ B "Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province." , ss.898 - 907, 2014.
ISNAD ALPER, MEHLİKA vd. "Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province". (2014), 898-907.
APA ALPER M, güneş h, Tatlıpınar A, ÇÖL B, CİVELEK H, ÖZKAN C, POYRAZ B (2014). Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. Turkish Journal of Agriculture and Forestry, 38(6), 898 - 907.
Chicago ALPER MEHLİKA,güneş hatice,Tatlıpınar Arzu,ÇÖL Bekir,CİVELEK HASAN SUNGUR,ÖZKAN Cem,POYRAZ Burcu Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. Turkish Journal of Agriculture and Forestry 38, no.6 (2014): 898 - 907.
MLA ALPER MEHLİKA,güneş hatice,Tatlıpınar Arzu,ÇÖL Bekir,CİVELEK HASAN SUNGUR,ÖZKAN Cem,POYRAZ Burcu Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. Turkish Journal of Agriculture and Forestry, vol.38, no.6, 2014, ss.898 - 907.
AMA ALPER M,güneş h,Tatlıpınar A,ÇÖL B,CİVELEK H,ÖZKAN C,POYRAZ B Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. Turkish Journal of Agriculture and Forestry. 2014; 38(6): 898 - 907.
Vancouver ALPER M,güneş h,Tatlıpınar A,ÇÖL B,CİVELEK H,ÖZKAN C,POYRAZ B Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province. Turkish Journal of Agriculture and Forestry. 2014; 38(6): 898 - 907.
IEEE ALPER M,güneş h,Tatlıpınar A,ÇÖL B,CİVELEK H,ÖZKAN C,POYRAZ B "Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province." Turkish Journal of Agriculture and Forestry, 38, ss.898 - 907, 2014.
ISNAD ALPER, MEHLİKA vd. "Distribution, occurrence of cry genes, and lepidopteran toxicity of native Bacillus thuringiensis isolated from fg tree environments in Aydın Province". Turkish Journal of Agriculture and Forestry 38/6 (2014), 898-907.
Sayfa Oluşturulma Tarihi
22-04-2024 20:31

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