Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats

Aliye SAĞKAN ÖZTÜRK, (Mustafa Kemal Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Hatay, Türkiye)
İsmail AYTEKİN, (Balıkesir Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Balıkesir, Türkiye)
Şule Yurdagül ÖZSOY, (Mustafa Kemal Üniversitesi, Veteriner Fakültesi, Patoloji Anabilim Dalı, Hatay, Türkiye)
Oktay Hasan ÖZTÜRK, (Akdeniz Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Antalya, Türkiye)
Nuri ALTUĞ, (Mustafa Kemal Üniversitesi, Veteriner Fakültesi, İç Hastalıkları Anabilim Dalı, Hatay, Türkiye)
Nigar YILMAZ (Muğla Sıtkı Koçman Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Muğla, Türkiye)
Türk Biyokimya Dergisi
10 3
Yıl: 2015 Cilt: 40 Sayı: 2 Sayfa Aralığı: 149 - 156 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats

Öz:
Amaç: Diabetes mellitus (DM) önemli bir halk sağlığı problemidir ve tedavisinde insulin terapisinin yanısıra bitkisel ve sentetik ilaçlar verilmektedir. Bu çalışmanın amacı, streptozotocin (STZ) ile indüklenen diyabetli sıçanlarda histopatolojik ve biyokimyasal metotlar kullanılarak CAPE'nin tedavi edici etkilerini değerlendirmektir.Metod: 22 yetişkin erkek Wistar Albino ırkı sıçan 3 gruba ayrıldı: kontrol grubu (n=8), diyabetik grup (n=6) ve diyabetik+CAPE grubu (n=8). 2 gruba periton içi (ip) 50 mg/kg STZ enjekte edilerek DM uyarıldı. Üç gün sonra poliüri ve polidipsi belirtileri ile kan glikoz seviyeleri 270 mg/dl ya da daha yüksek olan sıçanlar diyabetik olarak kabul edildi. Diyabetik+CAPE grubuna tedavi için CAPE 60 gün boyunca 10 µmol/kg dozunda ip olarak uygulandı.Bulgular: Karaciğer malondialdehit (MDA) seviyeleri diyabetik grupta önemli derecede yüksekti. Yüksek karaciğer MDA seviyesi, yüksek alanin aminotransferaz (ALT) ve aspartat aminotransferaz (AST) enzim seviyeleri tarafından desteklendi. Böbrek dokusundaki MDA seviyesinin diğer gruplar ile arasında önemli bir farklılık yoktu ve süperoksit dismutaz (SOD) seviyeleri CAPE grubunda artmıştı. İlave olarak kan üre nitrojen (BUN) ve kreatinin düzeyleri serum örneklerinde sabit kaldı. Deneyin sonunda pankreas dokusunda MDA seviyeleri kontrol grubuyla karşılaştırıldığında deneme gruplarında düşüktü. Böbrek, karaciğer ve pankreas dokularının histopatolojik sonuçları, diyabetik grupla karşılaştırıldığında CAPE grubunda normal histolojik yapıdaydı. Kan glikoz seviyeleri göz önüne alındığında, CAPE ile tedavi edilen grupta, diyabet grubundaki yüksek glikoz seviyeleri ile karşılaştırıldığında kontrol grubuna benzer seviyelerde kan glikozu muhafaza edilmişti. Sonuç: DM'un sebep olduğu oksidatif stres tarafından en çok etkilenen karaciğer dokusudur. Tek seferde STZ enjeksiyonu ile deneme sonunda pankreatik dokuda MDA seviyeleri azaldı. Bu azalma, doku bütünlüğünün bozulmasından kaynaklanan MDA'nın tükenmesi olabilirdi. Sonuç olarak, CAPE'nin oksidatif stresi baskıladığı ve STZ ile uyarılan DM'da kan glikoz düzeylerini düşürdüğü gözlemlendi. Sonuç olarak, DM'da antidiyabetik ilaçlara ilave olarak antioksidan özellikli ilaçları kullanmak faydalı olabilir.
Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji

[Streptozotocin ile indüklenen diyabetik sıçanlarda oksidatif stres, histopatolojik ve bazı biyokimyasal parametrelere kafeik asit fenetil esterin etkileri]

Öz:
Objective: Diabetes mellitus (DM) is a significant health problem and its treatmet has taken herbal and syntetic remedies as well as insulin therapy. The aim of this study was to evaluate the therapeutic effects of caffeic acid phenil ester (CAPE) in rats with streptozotocin (STZ)-induced diabetes using hystopathological and biochemical methods.Methods: 22 adult Wistar Albino strain male rats were divided into three groups: control group (n=8), diabetic group (n=6) and the diabetic+CAPE group (n=8). Two groups were injected intraperitoneally (i.p.) with 50 mg/kg STZ to induce DM. Rats with blood glucose levels of 270 mg/dL or greater and with siagns of polyuria and polydipsia after 3 days were considered to be diabetic. Diabetic+CAPE group was given ip CAPE (10 µmol/kg/during 60 days) for treatment.Results: Liver malondialdehyde (MDA) level was significantly higher in the diabetic group. The higher liver MDA was supported by higher enzyme levels, alanine aminotransferase (ALT) and aspartate aminotransferase (AST). MDA levels in kidney were not significantly different between groups and superoxide dismutase (SOD) levels were increased in CAPE group. Additionally, blood urea nitrogen (BUN) and creatinine enzyme levels were mostly stable in serum samples. At the end of the experiment, pancreatic tissue MDA in the experimental groups decreased compared with the control group. The kidney, liver and pancreas had almost normal histological structure in the CAPE group when compared to the diabetic group. Considering blood glucose levels, CAPE treatment maintained blood glucose at the same level with controls, compared to diabetic group.Conclusion: Liver tissue might be the most affected by oxidative stress caused DM. A single injection of STZ decreased the level of MDA in pancreatic tissue at the end of experiment. This decrease might be exhausting MDA due to disruption in tissue integrity. Consequently, it was observed that CAPE suppressed oxidative stress and decreased glucose levels in STZ-induced diabetic rats. Thus, it may be useful to use as remedies, the antioxidant properties, in addition to anti-diabetic drugs in DM.
Anahtar Kelime:

Konular: Biyokimya ve Moleküler Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA sagkan ozturk a, Aytekin İ, ÖZSOY Ş, ÖZTÜRK O, altuğ n, Yılmaz N (2015). Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. , 149 - 156.
Chicago sagkan ozturk aliye,Aytekin İsmail,ÖZSOY Şule Yurdagül,ÖZTÜRK Oktay Hasan,altuğ nuri,Yılmaz Nigar Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. (2015): 149 - 156.
MLA sagkan ozturk aliye,Aytekin İsmail,ÖZSOY Şule Yurdagül,ÖZTÜRK Oktay Hasan,altuğ nuri,Yılmaz Nigar Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. , 2015, ss.149 - 156.
AMA sagkan ozturk a,Aytekin İ,ÖZSOY Ş,ÖZTÜRK O,altuğ n,Yılmaz N Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. . 2015; 149 - 156.
Vancouver sagkan ozturk a,Aytekin İ,ÖZSOY Ş,ÖZTÜRK O,altuğ n,Yılmaz N Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. . 2015; 149 - 156.
IEEE sagkan ozturk a,Aytekin İ,ÖZSOY Ş,ÖZTÜRK O,altuğ n,Yılmaz N "Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats." , ss.149 - 156, 2015.
ISNAD sagkan ozturk, aliye vd. "Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats". (2015), 149-156.
APA sagkan ozturk a, Aytekin İ, ÖZSOY Ş, ÖZTÜRK O, altuğ n, Yılmaz N (2015). Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. Türk Biyokimya Dergisi, 40(2), 149 - 156.
Chicago sagkan ozturk aliye,Aytekin İsmail,ÖZSOY Şule Yurdagül,ÖZTÜRK Oktay Hasan,altuğ nuri,Yılmaz Nigar Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. Türk Biyokimya Dergisi 40, no.2 (2015): 149 - 156.
MLA sagkan ozturk aliye,Aytekin İsmail,ÖZSOY Şule Yurdagül,ÖZTÜRK Oktay Hasan,altuğ nuri,Yılmaz Nigar Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. Türk Biyokimya Dergisi, vol.40, no.2, 2015, ss.149 - 156.
AMA sagkan ozturk a,Aytekin İ,ÖZSOY Ş,ÖZTÜRK O,altuğ n,Yılmaz N Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. Türk Biyokimya Dergisi. 2015; 40(2): 149 - 156.
Vancouver sagkan ozturk a,Aytekin İ,ÖZSOY Ş,ÖZTÜRK O,altuğ n,Yılmaz N Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats. Türk Biyokimya Dergisi. 2015; 40(2): 149 - 156.
IEEE sagkan ozturk a,Aytekin İ,ÖZSOY Ş,ÖZTÜRK O,altuğ n,Yılmaz N "Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats." Türk Biyokimya Dergisi, 40, ss.149 - 156, 2015.
ISNAD sagkan ozturk, aliye vd. "Effects of caffeic acid phenethyl ester on oxidative stress, hystopathology and some biochemical parameters in streptozotocin-induced diabetic rats". Türk Biyokimya Dergisi 40/2 (2015), 149-156.
Sayfa Oluşturulma Tarihi
22-04-2024 23:59

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