HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey

Tüney, İpek; BOZDAYI, Gülendam; USUBÜTÜN, Alp; Altay Kocak, Aylin; Yuce, Kunter; BADUR, Osman Selim; PINAR, AHMET; salman, mehmet coskun; ERGÜNAY, KORAY; KARABULUT, Erdem; ÖNDER, SEVGEN ÇELİK

HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey

İpek TÜNEY, (Hacettepe Üniversitesi Tıp Fakültesi, Viroloji Bölümü Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

AYLİN ALTAY KOÇAK, (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Koray ERGÜNAY, (Hacettepe Üniversitesi Tıp Fakültesi, Viroloji Bölümü Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Sevgen Çelik ÖNDER, (Hacettepe Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Ankara, Türkiye)

Alp USUBÜTÜN, (Hacettepe Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Ankara, Türkiye)

Mehmet Coşkun SALMAN, (Hacettepe Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum AD, Ankara, Türkiye)

Gülendam BOZDAYI, (Gazi Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Erdem KARABULUT, (Hacettepe Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum AD, Ankara, Türkiye)

Osman Selim BADUR, (İstanbul Üniversitesi İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Kunter YÜCE, (Hacettepe Üniversitesi Tıp Fakültesi Kadın Hastalıkları ve Doğum AD, Ankara, Türkiye)

Ahmet PINAR, (Hacettepe Üniversitesi Tıp Fakültesi, Viroloji Bölümü Tıbbi Mikrobiyoloji Anabilim Dalı, Ankara, Türkiye)

Diğer Yazarlar

Turkish Journal of Medical Sciences

5 1

Yıl: 2017 Cilt: 47 Sayı: 1 Sayfa Aralığı: 194 - 200 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey

Öz:

Background/aim: Human papillomaviruses have been established as a risk factor for invasive carcinoma of the uterine cervix. HPV E6/ E7 oncogene expression has recently emerged as a promising biomarker to determine the risk for progression to high-grade cervical lesions. The aim of this study was to evaluate HPV mRNA and DNA detection in samples with abnormal cytology. Materials and methods: Cervical specimens were obtained at the Department of Obstetrics and Gynecology via cervical brushes during January October 2011. Liquid-based cytology slides were evaluated according to the 2001 Bethesda System. Cytology specimens from a total of 81 women with abnormal cytology were included. Real-time PCR and NASBA assays were performed to detect HPV DNA and E6/E7 mRNA, respectively. Results: HPV DNA was identified in 73 samples (90.1%). HPV E6/E7 mRNA expression was observed in 45 samples (55.6%). A statistically significant difference was observed among cytological diagnosis groups. In 25 patients, a biopsy was performed during the follow-up. HPV DNA was detected in all of these patients. HPV E6/E7 expression was present only in CIN I III diagnosed patients. Conclusion: The E6/E7 mRNA test is a robust indicator of cytological atypia and correlates better with progressive lesions than DNA assays.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Tüney İ, Altay Kocak A, ERGÜNAY K, ÖNDER S, USUBÜTÜN A, salman m, BOZDAYI G, KARABULUT E, BADUR O, Yuce K, PINAR A (2017). HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. , 194 - 200.
Chicago	Tüney İpek,Altay Kocak Aylin,ERGÜNAY KORAY,ÖNDER SEVGEN ÇELİK,USUBÜTÜN Alp,salman mehmet coskun,BOZDAYI Gülendam,KARABULUT Erdem,BADUR Osman Selim,Yuce Kunter,PINAR AHMET HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. (2017): 194 - 200.
MLA	Tüney İpek,Altay Kocak Aylin,ERGÜNAY KORAY,ÖNDER SEVGEN ÇELİK,USUBÜTÜN Alp,salman mehmet coskun,BOZDAYI Gülendam,KARABULUT Erdem,BADUR Osman Selim,Yuce Kunter,PINAR AHMET HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. , 2017, ss.194 - 200.
AMA	Tüney İ,Altay Kocak A,ERGÜNAY K,ÖNDER S,USUBÜTÜN A,salman m,BOZDAYI G,KARABULUT E,BADUR O,Yuce K,PINAR A HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. . 2017; 194 - 200.
Vancouver	Tüney İ,Altay Kocak A,ERGÜNAY K,ÖNDER S,USUBÜTÜN A,salman m,BOZDAYI G,KARABULUT E,BADUR O,Yuce K,PINAR A HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. . 2017; 194 - 200.
IEEE	Tüney İ,Altay Kocak A,ERGÜNAY K,ÖNDER S,USUBÜTÜN A,salman m,BOZDAYI G,KARABULUT E,BADUR O,Yuce K,PINAR A "HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey." , ss.194 - 200, 2017.
ISNAD	Tüney, İpek vd. "HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey". (2017), 194-200.

APA	Tüney İ, Altay Kocak A, ERGÜNAY K, ÖNDER S, USUBÜTÜN A, salman m, BOZDAYI G, KARABULUT E, BADUR O, Yuce K, PINAR A (2017). HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. Turkish Journal of Medical Sciences, 47(1), 194 - 200.
Chicago	Tüney İpek,Altay Kocak Aylin,ERGÜNAY KORAY,ÖNDER SEVGEN ÇELİK,USUBÜTÜN Alp,salman mehmet coskun,BOZDAYI Gülendam,KARABULUT Erdem,BADUR Osman Selim,Yuce Kunter,PINAR AHMET HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. Turkish Journal of Medical Sciences 47, no.1 (2017): 194 - 200.
MLA	Tüney İpek,Altay Kocak Aylin,ERGÜNAY KORAY,ÖNDER SEVGEN ÇELİK,USUBÜTÜN Alp,salman mehmet coskun,BOZDAYI Gülendam,KARABULUT Erdem,BADUR Osman Selim,Yuce Kunter,PINAR AHMET HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. Turkish Journal of Medical Sciences, vol.47, no.1, 2017, ss.194 - 200.
AMA	Tüney İ,Altay Kocak A,ERGÜNAY K,ÖNDER S,USUBÜTÜN A,salman m,BOZDAYI G,KARABULUT E,BADUR O,Yuce K,PINAR A HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. Turkish Journal of Medical Sciences. 2017; 47(1): 194 - 200.
Vancouver	Tüney İ,Altay Kocak A,ERGÜNAY K,ÖNDER S,USUBÜTÜN A,salman m,BOZDAYI G,KARABULUT E,BADUR O,Yuce K,PINAR A HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey. Turkish Journal of Medical Sciences. 2017; 47(1): 194 - 200.
IEEE	Tüney İ,Altay Kocak A,ERGÜNAY K,ÖNDER S,USUBÜTÜN A,salman m,BOZDAYI G,KARABULUT E,BADUR O,Yuce K,PINAR A "HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey." Turkish Journal of Medical Sciences, 47, ss.194 - 200, 2017.
ISNAD	Tüney, İpek vd. "HPV types and E6/E7 mRNA expression in cervical samples from Turkish women with abnormal cytology in Ankara, Turkey". Turkish Journal of Medical Sciences 47/1 (2017), 194-200.