Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures

TAMER, A. Üsame; Sahin, Nurettin

Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures

Nurettin ŞAHİN, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Muğla, Türkiye)

A. Üsame TAMER (Girilmedi)

Turkish Journal of Biology

1 0

Yıl: 2000 Cilt: 24 Sayı: 2 Sayfa Aralığı: 353 - 363 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures

Öz:

Karısık mikrobiyal kültürler Gediz havzasından alınan toprak örneklerinden zenginlestirme ile elde edildi. Fungusid thiram içeren zenginlestirilmis kültürlerden fungus ve bakteri türleri izole edildi ve kısmen tanımlandı. Morfolojik ve biyokimyasal testlere verdikleri cevaplara göre funguslar; Aspergillus niger, A. flavus ve Penicillium steckii, olarak tanımlandı. Bakteriyal izolatlar; Bacillus, Arthrobacter, Moraxella-like Acinetobacter ve Streptomyces genuslarına dahil edildi. Izolatlar 0.2 mg/ml thiram içeren mineral besiyerinde gelistiler ve büyüme faktörlerine ihtiyaç duymazlar.

Anahtar Kelime: biyodegradasyon mantar öldürücüler Bacillus zararlı öldürücüler Aspergillus flavus Penicillium toprak Arthrobacter Acinetobacter tanı Streptomyces tiram mikroorganizmalar Aspergillus niger fenoloji karakterizasyon izolasyon

Konular: Biyoloji

Zenginleştirilmiş toprak kültüründen thiramı paçalayan mikroorganizmaların izolasyonu, karakterizasyonu ve tanımlanması

Öz:

Mixed microbial cultures were obtained by enrichment from soil samples collected from the Gediz basin. A number of species of bacteria and fungi were isolated and partially characterized from enrichment clutures containing the fungicide thiram. According to their responses in morphological and biochemical tests fungi were identified as Aspergillus niger, A. flavus and Penicilium steckii. Bacterial isolates were assigned to the genera: Bacillus, Arthrobacter, Moraxella-like Acinetobacter and Streptomyces. Islates grew in a mineral salts medium containing 0.2 mg/ml thiram and no growth factors were required.

Anahtar Kelime: Streptomyces thiram microorganisms Aspergillus niger phenology characterization isolation biodegradation fungicides Bacillus pesticides Aspergillus flavus Penicillium soil Arthrobacter Acinetobacter identification

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Diğer Erişim Türü: Erişime Açık

1. Worthing, C. R., Hance, R. J.: The pesticide manual, 9th Ed. Published by the British Crop Protection Council, 1991, 822-823. 2. Duffield, W. J., Eide, R. R., Application of rabbit repellent to coniferous planting stock in the Pacific Northwest. J. Forest 60: 109-111, 1962. 3. Richardson, L. T., The persistance of thiram in soil and its relationship to the microbiological balance and damping -off control. Canad. J. Bot. 32: 335-346, 1954. 4. Raghu, K., Murthy, N. B. K., Kumaraswamy, R., Rao, R. S., Sane, P. V.: Proceedings of the joint FAO/IAEA. Division of atomic energy in food and agriculture, Vienna, 1975, 137. 5. Shirkot, C. K.: In studies on the interaction of soil microorganisms and dithiocarbamates. Ph. D. thesis, Punjab University, Chandigarh, India, 1983, 48-60. 6. Shirkot, C. K., Gupta, K. G., Accelerated tetramethylthiuram disulfide (TMTD) degradation in soil by inoculation with TMTD-utilizing bacteria. Bull. Environ. Contam. Toxicol. 35: 354-361, 1985. 7. Shirkot, C. K., Shirkot, P., Dhall, S. P., Gupta, K. G., Effectiveness of Pseudomonas aeruginosa for detoxificaton of 8. Klages, U., Lingens, F., Degradation of 4-chlorobenzoic acid by a Pseudomonas sp. Zenterblatt. Bakt. Hyg., I. Abt. Orig. C 1: 215-223, 1980. 9. Edwards, D. E., Kremer, R. J., Keaster, A. J., Characterization and growth response of bacteria in soil following application of carbofuran. J. Environmen. Sci. Health. B 27: (2), 139-154, 1992. 10. Steneck, J. L., Roberts, G. D., Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl. Microbiol. 28: 226-231, 1974. 11. Staley, J. T., Bryant, M. P., Pfenning, N., Hotl, J. G.: Bergey’s manual of systematic bacteriology. vol. 1-4. Williams & Wilkins, Baltimore, Md., 1984. 12. Ellis, M. B.: Dematiaceaus hyphomycetes. Kew, Commonwealth mycological Institute, 1971. 13. Domsch, K. H., Gams, W., Anderson, T. H.: Compendium of soil fungi. London, 1980, Academic press. 14. Arx, J. A.: The genera of fungi sporulating in pure culture. 1981, Vaduz J. Cramer, 1-335. 15. AOAC : Official Methods of Analysis of the Association of Official Analytical Chemists. (Ed. Williams S.), Arlington, Virginia, 1990, Association Official Analytical Chemists, Inc. 16. Wollum, A. G.: Cultural methods for soil microorganisms, In: Page, A. L (ed) Methods of soil analysis, part 2, 1982, Madison, Wisconsin, American Society for Agronomy, 781. 17. Sokal, R. R., Michener, C. D., A statistical method for evaluating systematic relationships. Univ. Kansas Sci. Bull., 38: 1409-1438, 1958. 18. Jaccard, P., Nouvelles recherches sur la distribution florale. Bull. Soc. Vaud. Sci. Nat. 44: 226-270, 1908. 19. Sneath, P. H. A., Some thoughts on bacterial classification. J. Gen. Microbiol., 17: 184-200, 1957. 20. Sneath, P. H. A., The application of computers to taxonomy. J. Gen. Microbiol., 17: 206-226, 1957. 21. Wallnöfer, P. R., Engelhardt, G.: Microbial degradation of pesticides. Chemistry of plant protection 2, Berlin- Heidelberg, 1989, Spinger-Verlag, 1-115. 22. Nelson, L. M., Biologycally-induced hydrolysis of parathion in soil: isolation of hydrolyzing bacteria. Soil Biol. Biochem. 14: 219-222, 1982. 23. Barlas (Emir), N., The effects of commercial and microorganism-degraded solutions of endosulfan and carbaryl on albino mice. Tr. J. of Zoology. 18: 221-226, 1994. 24. Chen, T. C., Levin, R. E., Taxonomic significance of phenethyl alcohol production by Achromobacter isolated from fishery sources. Appl. Microbiol. 28: 681-687, 1974. 25. Lee, A., EPTC (S-ethyl N, N-dipropylthiocarbamate) degrading microorganisms isolated from a soil previously exposed to EPTC. Soil Biol. Biochem., 16: (5), 529-531, 1984. 26. Wong, D. H., Kirkpatrick, W. E., King, D. R., Kinnear, J. E., Defluorination of sodium monofluoroacetate (1080) by microorganisms isolated from western Australian soils. Soil Biol. Biochem. 24: (9), 833-838, 1992. 27. Elskens, M. T., Penninckx, M. J., Thiram and dimethyldithiocarbamic acid interconversion in Saccharomyces cerevisiae: a possible metabolic pathway under the control of the glutathione redox cycle. Appl. Environ. Microbiol. 63: 7, 2857-2862, 1997.

APA	Sahin N, TAMER A (2000). Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. , 353 - 363.
Chicago	Sahin Nurettin,TAMER A. Üsame Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. (2000): 353 - 363.
MLA	Sahin Nurettin,TAMER A. Üsame Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. , 2000, ss.353 - 363.
AMA	Sahin N,TAMER A Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. . 2000; 353 - 363.
Vancouver	Sahin N,TAMER A Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. . 2000; 353 - 363.
IEEE	Sahin N,TAMER A "Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures." , ss.353 - 363, 2000.
ISNAD	Sahin, Nurettin - TAMER, A. Üsame. "Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures". (2000), 353-363.

APA	Sahin N, TAMER A (2000). Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. Turkish Journal of Biology, 24(2), 353 - 363.
Chicago	Sahin Nurettin,TAMER A. Üsame Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. Turkish Journal of Biology 24, no.2 (2000): 353 - 363.
MLA	Sahin Nurettin,TAMER A. Üsame Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. Turkish Journal of Biology, vol.24, no.2, 2000, ss.353 - 363.
AMA	Sahin N,TAMER A Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. Turkish Journal of Biology. 2000; 24(2): 353 - 363.
Vancouver	Sahin N,TAMER A Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures. Turkish Journal of Biology. 2000; 24(2): 353 - 363.
IEEE	Sahin N,TAMER A "Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures." Turkish Journal of Biology, 24, ss.353 - 363, 2000.
ISNAD	Sahin, Nurettin - TAMER, A. Üsame. "Isolation, characterization and identification of thiram-degrading microorganisms from soil enrichment cultures". Turkish Journal of Biology 24/2 (2000), 353-363.