The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen

Balcı, Uydu; BAYGAR, Tuba; Sarac, Nurdan; BAYGAR, TAÇNUR; Ugur, Aysel

The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen

Tuba BAYGAR, (Material Research Laboratory, Research Laboratories Center, Muğla Sıtkı Koçman University, Muğla, Turkey)

Nurdan SARAÇ, (Mugla Sitki Kocman University, Faculty of Science, Department of Biology, Muğla-TURKEY)

Aysel UĞUR, (Section of Medical Microbiology, Department of Basic Sciences, Faculty of Dentistry, Gazi University, Ankara, Turkey)

Taçnur BAYGAR, (Mugla Sitki Kocman University, Faculty of Fisheries, Department of Seafood Processing Technology, Muğla-TURKEY)

Uydu BALCI (Mugla Sitki Kocman University, Faculty of Science, Department of Biology, Muğla-TURKEY)

Erciyes Üniversitesi Veteriner Fakültesi Dergisi

7 1

Yıl: 2019 Cilt: 16 Sayı: 1 Sayfa Aralığı: 23 - 29 Metin Dili: İngilizce İndeks Tarihi: 23-07-2019

The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen

Öz:

Aerobic, non-fermentative and Gram-negative Stenotrophomonas maltophilia is a multidrug-resistant bacilli that is known to be pathogen for human and animals. S. maltophilia has been isolated from different animal species and also found in a variety of environments including soil, water, and plants. S. maltophilia, which has the ability to form biofilms on surfaces that cause environmental problems, is resistant to many antibiotic classes such as cephalosporins, carbapenems, and aminoglycosides. Here in this study, its aimed to determine the inhibition activities of natural phenolic compounds eugenol and pulegone against S. maltophilia MU69. Antibacterial activities of eugenol and pulegone were initially determined by disc diffusion method. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) were also determined by tube dilution assay. Antibiofilm activities of the compounds were investigated by crystal violet staining and also monitorized using Scanning Electron Microscopy (SEM). Zone of inhibition measurements were found to be 39 mm and 28 mm for eugenol and pulegone, respectively. MIC and MBC values were found to be 3.12 mg/mL for eugenol while these were 2.5 mg/mL and 5 mg/mL for pulegone, respectively. The highest antibiofilm activity was found to be 56.30±0.45% for MIC of eugenol. To our knowledge, this is the first report of the antibacterial and antibiofilm activity of eugenol and pulegone against S. maltophilia. According to the biological activity results, it can be concluded that these natural agents may be potentially used for veterinary sciences, food industry or pharmaceutical applications that aim to manage S. maltophilia biofilm.

Anahtar Kelime:

Konular: Veterinerlik

Eugenol ve Pulegonun Fırsatçı Patojen Bir Bakteri Olan Stenotrophomonas maltophilia’ya Karşı İnhibisyon Etkinliğinin Belirlenmesi

Öz:

Aerobik, non-fermentatif ve gram-negatif Stenotrophomonas maltophilia, insan ve hayvanlar için patojen olarak bilinen çoklu ilaç direncine sahip bir basildir. Farklı hayvan türlerinden izole edilmiş S. maltophilia suşu toprak, su ve bitkiler gibi çeşitli çevresel etmenlerden de izole edilebilmektedir. Katı yüzeyler üzerinde biyofilm oluşturabilme yeteneği sayesinde S. maltophilia suşu çevre sorunlarına yol açmakla birlikte aminoglikozitler, karbapenemler ve sefalosporinler gibi çeşitli antibiyotik sınıflarına karşı direnç göstermektedir. Bu çalışmada doğal fenolik bileşikler olan eugenol ve pulegonun S. maltophila MU69 suşu üzerindeki inhibisyon aktivitelerinin belirlenmesi amaçlanmıştır. Eugenol ve pulegonun antibakteriyel aktivitesi disk difüzyon yöntemi ile araştırılmıştır. Minimum İnhibisyon Konsatrasyonu (MİK) ve Minimum Bakterisidal Konsantrasyon (MBK) ise tüp dilüsyon yöntemi ile belirlenmiştir. Antibiyofilm aktivitenin belirlenmesinde kristal viyole boyama yöntemi uygulanmış ve ayrıca Taramalı Elektron Mikroskobi (SEM) ile görüntülenmiştir. Eugenol ve pulegon için inhibisyon zonu sırasıyla 39 mm ve 28 mm olarak ölçülmüştür. MİK ve MBK değerleri eugenol için 3.12 mg/mL olarak belirlenirken, pulegon için sırasıyla 2.5 mg/mL ve 5 mg/mL olarak tespit edilmiştir. En yüksek antibiyofilm aktivite oranı % 56.30±0.45 ile eugenole ait MİK değeri için gözlenmiştir. Bu çalışma eugenol ve pulegon bileşiklerinin S. maltophilia suşuna karşı antibakteriyel ve antibiyofilm aktivitelerinin belirlendiği ilk çalışma olarak sunulmaktadır. Biyolojik aktivite çalışmalarının sonuçlarına göre bu iki doğal bileşik veteriner bilimleri, gıda endüstrisi ve farmasötik uygulamalarda S. maltophilia’ya karşı geliştirilen sistemlerde potansiyel kullanım kapasitesine sahiptir.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BAYGAR T, Sarac N, Ugur A, BAYGAR T, Balcı U (2019). The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. , 23 - 29.
Chicago	BAYGAR Tuba,Sarac Nurdan,Ugur Aysel,BAYGAR TAÇNUR,Balcı Uydu The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. (2019): 23 - 29.
MLA	BAYGAR Tuba,Sarac Nurdan,Ugur Aysel,BAYGAR TAÇNUR,Balcı Uydu The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. , 2019, ss.23 - 29.
AMA	BAYGAR T,Sarac N,Ugur A,BAYGAR T,Balcı U The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. . 2019; 23 - 29.
Vancouver	BAYGAR T,Sarac N,Ugur A,BAYGAR T,Balcı U The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. . 2019; 23 - 29.
IEEE	BAYGAR T,Sarac N,Ugur A,BAYGAR T,Balcı U "The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen." , ss.23 - 29, 2019.
ISNAD	BAYGAR, Tuba vd. "The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen". (2019), 23-29.

APA	BAYGAR T, Sarac N, Ugur A, BAYGAR T, Balcı U (2019). The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16(1), 23 - 29.
Chicago	BAYGAR Tuba,Sarac Nurdan,Ugur Aysel,BAYGAR TAÇNUR,Balcı Uydu The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. Erciyes Üniversitesi Veteriner Fakültesi Dergisi 16, no.1 (2019): 23 - 29.
MLA	BAYGAR Tuba,Sarac Nurdan,Ugur Aysel,BAYGAR TAÇNUR,Balcı Uydu The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. Erciyes Üniversitesi Veteriner Fakültesi Dergisi, vol.16, no.1, 2019, ss.23 - 29.
AMA	BAYGAR T,Sarac N,Ugur A,BAYGAR T,Balcı U The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2019; 16(1): 23 - 29.
Vancouver	BAYGAR T,Sarac N,Ugur A,BAYGAR T,Balcı U The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen. Erciyes Üniversitesi Veteriner Fakültesi Dergisi. 2019; 16(1): 23 - 29.
IEEE	BAYGAR T,Sarac N,Ugur A,BAYGAR T,Balcı U "The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen." Erciyes Üniversitesi Veteriner Fakültesi Dergisi, 16, ss.23 - 29, 2019.
ISNAD	BAYGAR, Tuba vd. "The Inhibition Effects of Eugenol and Pulegone on Stenotrophomonas maltophilia: an Opportunistic Pathogen". Erciyes Üniversitesi Veteriner Fakültesi Dergisi 16/1 (2019), 23-29.