Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources

Sahin, Nurettin; Ugur, Aysel; Beyatlı, Yavuz

Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources

Aysel UĞUR, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Kimya Bölümü, Muğla, Türkiye)

Nurettin ŞAHİN, (Girilmedi)

Yavuz BEYATLI (Girilmedi)

Turkish Journal of Biology

1 0

Yıl: 2002 Cilt: 26 Sayı: 3 Sayfa Aralığı: 171 - 174 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources

Öz:

Farklı taksonomik özelliklere sahip 27 Streptomyces izolatında Poly--hydroxybutyric asit (PHB) birikimi araştırıldı. Sentezlenen PHB miktarı krotonik asit olarak spektrofotometre ile belirlendi. Denenen izolatların %80’i PHB yi kuru miselyum ağırlığının %0.3-7.6’sı arasında akümüle etti. PHB, seçilmiş Streptomyces izolatları tarafından, farklı azot kaynakları (KNO3, glisin, pepton, proteoz pepton, L-asparagin, tripton ve malt ekstrakt) ve karbon kaynağı olarak da herbiri 2 g/l glukoz içeren besiyerlerinde üretildi. En etkili PHB üretimi kuru miselyum ağırlığının %7.6’sı ile glisin/glukoz ortamında gözlendi. Azotca sınırlandırılmış şartlar Streptomyces gelişimini inhibe ederken, PHB akümülasyonunu teşvik etmiştir. Morfolojik ve biyokimyasal test sonuçlarına göre seçilen izolatlar, Streptomyces halstedii (MU112), S. anulatus (MU117) ve S. rochei (MU119) olarak tanımlandı.

Anahtar Kelime: taksonomi kuru madde birikimi polihidroksibütirat biyokimyasal teknikler kültür ortamı Streptomyces anulatus Streptomyces halstedii bakteriler Streptomyces birikim azot morfoloji Streptomyces rochei miselyum

Konular: Biyoloji

Farklı azot kaynakları ile büyüme sırasında Streptomyces türlerinde poly-$beta$- hydroxybutyratın birikimi

Öz:

The accumulation of poly-b-hydroxybutyric acid (PHB) in 27 Streptomyces isolates with different taxonomical properties was investigated. The amount of synthesized PHB was determined as crotonic acid by spectrophotometry. Eighty percent of the tested isolates accumulated PHB between 0.3 and 7.6% of dry mycelial weight. PHB was produced by a selectant of Streptomyces isolates in media containing different nitrogen sources (KN$O_3$, glycine, peptone, proteose peptone, L-asparagine, tryptone and malt extract), each combined with 2 g/l glucose as the carbon source. The most effective PHB production was observed on glycine/glucose medium with a 7.6% dry mycelial weight. Nitrogen limiting conditions were inhibitory to Streptomyces growth, but stimulated PHB accumulation. Based on the morphological and biochemical test results, selected isolates were identified as Streptomyces halstedii (MU112), S. anulatus (MU117) and S. rochei (MU119).

Anahtar Kelime: Streptomyces halstedii bacteria Streptomyces accumulation nitrogen morphology Streptomyces rochei mycelium taxonomy dry matter accumulation polyhydroxybutyrate biochemical techniques culture media Streptomyces anulatus

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Ugur A, Sahin N, Beyatlı Y (2002). Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. , 171 - 174.
Chicago	Ugur Aysel,Sahin Nurettin,Beyatlı Yavuz Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. (2002): 171 - 174.
MLA	Ugur Aysel,Sahin Nurettin,Beyatlı Yavuz Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. , 2002, ss.171 - 174.
AMA	Ugur A,Sahin N,Beyatlı Y Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. . 2002; 171 - 174.
Vancouver	Ugur A,Sahin N,Beyatlı Y Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. . 2002; 171 - 174.
IEEE	Ugur A,Sahin N,Beyatlı Y "Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources." , ss.171 - 174, 2002.
ISNAD	Ugur, Aysel vd. "Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources". (2002), 171-174.

APA	Ugur A, Sahin N, Beyatlı Y (2002). Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. Turkish Journal of Biology, 26(3), 171 - 174.
Chicago	Ugur Aysel,Sahin Nurettin,Beyatlı Yavuz Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. Turkish Journal of Biology 26, no.3 (2002): 171 - 174.
MLA	Ugur Aysel,Sahin Nurettin,Beyatlı Yavuz Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. Turkish Journal of Biology, vol.26, no.3, 2002, ss.171 - 174.
AMA	Ugur A,Sahin N,Beyatlı Y Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. Turkish Journal of Biology. 2002; 26(3): 171 - 174.
Vancouver	Ugur A,Sahin N,Beyatlı Y Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources. Turkish Journal of Biology. 2002; 26(3): 171 - 174.
IEEE	Ugur A,Sahin N,Beyatlı Y "Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources." Turkish Journal of Biology, 26, ss.171 - 174, 2002.
ISNAD	Ugur, Aysel vd. "Accumulation of poly-$beta$-hydroxybutyrate in Streptomyces species during growth with different nitrogen sources". Turkish Journal of Biology 26/3 (2002), 171-174.