Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides
Yıl: 2018 Cilt: 22 Sayı: 3 Sayfa Aralığı: 1182 - 1189 Metin Dili: İngilizce DOI: 10.19113/sdufenbed.471994 İndeks Tarihi: 05-03-2019
Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides
Öz: The aim of this study was to investigate the hyaluronidase, collagenase andelastase inhibitory effects, which play important role for wound healing, together withthe antibacterial, antioxidant and homeostatic activities of tragacanth gum (TG) andlocust bean gum (LBG). The antimicrobial activities were tested against four bacteriaand the antioxidant activities were estimated by the 1,1-diphenyl-2-picrylhydrazyl(DPPH), hydrogen peroxide (H2O2) radical scavenging and β-carotene bleachingassays. Homeostatic effect was evaluated with the Prothrombin Time (PT) andActivated Partial Thromboplastin Time (aPTT) test parameters. The wound healingpotentials were determined with the inhibition of hyaluronidase, collagenase andelastase enzymes. The TG showed antibacterial activity against Pseudomonasaeruginosa ATCC27853 and Escherichia coli ATCC25922. The results showed that TGand LBG possessed antioxidant properties including DPPH scavenging (21.0% and17.6%, respectively) and H2O2 radical scavenging (59.4% and 79.0%, respectively)activities. The polysaccharides displayed significantly reducing PT and aPTT results.Between the two tested polysaccharides LBG showed significant hyaluronidase andcollagenase inhibition activity at 10 mg/mL concentration. These findings show thatthese natural polysaccharides can be used to support of wound healing
Anahtar Kelime: İki Doğal Polisakkaritin Hiyalüronidaz, Kollajenaz ve Elastaz İnhibitör Potansiyellerinin Araştırılması ve Antimikrobiyal, Antioksidan ve Homeostatik Aktivitelerinin Karşılaştırmalı Olarak Değerlendirmesi
Öz: Bu çalışmanın amacı, kitre zamkı (TG) ve keçiboynuzu zamkı (LBG)'nin antibakteriyel, antioksidan ve homeostatik aktiviteleri ile birlikte yara iyileşmesi için önemli olan hiyalüronidaz, kollajenaz ve elastaz inhibitör etkilerini araştırmaktır. Antimikrobiyal aktiviteleri dört bakteriye karşı test edilerek, antioksidan aktiviteleri 1,1-difenil-2-pikrilhidrazil (DPPH), hidrojen peroksit (H202) radikal temizleme ve βkaroten ağartma deneyleri ile tespit edilmiştir. Homeostatik etki Protrombin Zamanı (PT) ve Aktive Edilen Kısmi Tromboplastin Zamanı (aPTT) test parametreleri ile değerlendirilmiştir. Yara iyileştirme potansiyelleri ise, hiyalüronidaz, kollajenaz ve elastaz inhibisyonu ile belirlenmiştir. TG, Pseudomonas aeruginosa ATCC27853 ve Escherichia coli ATCC25922'ye karşı antibakteriyel aktivite göstermiştir. Sonuçlar TG ve LBG'nin DPPH temizleme (sırasıyla %21.0 ve %17.6) ve H2O2 radikal temizleme (sırasıyla %59.4 ve %79.0) aktiviteleri de dahil olmak üzere antioksidan özelliklere sahip olduğunu göstermiştir. Polisakkaritler, PT ve aPTT'de önemli azalma göstermiştir. Test edilen iki polisakkarit arasından LBG, 10 mg/mL konsantrasyonda, önemli hiyalüronidaz ve kollajenaz inhibisyon aktivitesi göstermiştir. Bu bulgular, bu doğal polisakkaritlerin yara iyileşmesini desteklemek için kullanılabileceğini göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Boran R, Ugur A, Sarac N (2018). Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. , 1182 - 1189. 10.19113/sdufenbed.471994 |
| Chicago | Boran Rukiye,Ugur Aysel,Sarac Nurdan Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. (2018): 1182 - 1189. 10.19113/sdufenbed.471994 |
| MLA | Boran Rukiye,Ugur Aysel,Sarac Nurdan Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. , 2018, ss.1182 - 1189. 10.19113/sdufenbed.471994 |
| AMA | Boran R,Ugur A,Sarac N Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. . 2018; 1182 - 1189. 10.19113/sdufenbed.471994 |
| Vancouver | Boran R,Ugur A,Sarac N Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. . 2018; 1182 - 1189. 10.19113/sdufenbed.471994 |
| IEEE | Boran R,Ugur A,Sarac N "Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides." , ss.1182 - 1189, 2018. 10.19113/sdufenbed.471994 |
| ISNAD | Boran, Rukiye vd. "Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides". (2018), 1182-1189. https://doi.org/10.19113/sdufenbed.471994 |
| APA | Boran R, Ugur A, Sarac N (2018). Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22(3), 1182 - 1189. 10.19113/sdufenbed.471994 |
| Chicago | Boran Rukiye,Ugur Aysel,Sarac Nurdan Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22, no.3 (2018): 1182 - 1189. 10.19113/sdufenbed.471994 |
| MLA | Boran Rukiye,Ugur Aysel,Sarac Nurdan Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, vol.22, no.3, 2018, ss.1182 - 1189. 10.19113/sdufenbed.471994 |
| AMA | Boran R,Ugur A,Sarac N Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 22(3): 1182 - 1189. 10.19113/sdufenbed.471994 |
| Vancouver | Boran R,Ugur A,Sarac N Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides. Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi. 2018; 22(3): 1182 - 1189. 10.19113/sdufenbed.471994 |
| IEEE | Boran R,Ugur A,Sarac N "Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides." Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 22, ss.1182 - 1189, 2018. 10.19113/sdufenbed.471994 |
| ISNAD | Boran, Rukiye vd. "Investigation of Hyaluronidase, Collagenase and Elastase Inhibitory Potentials and Comparative Evaluation of the Antimicrobial, Antioxidant and Homeostatic Activities of Two Natural Polysaccharides". Süleyman Demirel Üniversitesi Fen Bilimleri Enstitüsü Dergisi 22/3 (2018), 1182-1189. https://doi.org/10.19113/sdufenbed.471994 |
