A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis
Yıl: 2018 Cilt: 19 Sayı: 3 Sayfa Aralığı: 205 - 210 Metin Dili: İngilizce DOI: 10.4274/meandros.48303 İndeks Tarihi: 11-09-2019
A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis
Öz: Objective: Alopecia areata (AA) is a disorder characterized by non-scarring hair loss, whose etiology involves oxidative stress. We aimed to determine the role of thiol/disulphide levels in AA pathogenesis and to investigate if they can be used as its marker. Materials and Methods: This prospective study included 100 AA patients who presented to dermatology outpatient clinic and 100 healthy controls without any systemic and/or inflammatory dermatological disorder. The control and study groups were compared with respect to native thiol, total thiol, and disulphide levels, and disulphide/native thiol, disulphide/total thiol, and native thiol/total thiol ratios. The relationships between demographic and lesion characteristics, native thiol, total thiol, and disulphide levels, and disulphide/native thiol, disulphide/total thiol, and native thiol/total thiol ratios were studied. Results: The mean age of AA patients was 37.5 years. Fifty-eight (58%) patients were male, and the median body mass index was 24 kg/m2. Median age was significantly higher in the AA group (p<0.05). The AA group had a significantly lower total and native thiol level, native thiol/total thiol ratio, significantly higher disulphide level, disulphide/native thiol ratio, and disulphide/total thiol ratio (p<0.05 for all comparisons). There was no correlation between the parameters of thiol/disulfide hemostasis and demographic and lesion characteristics (p>0.05). Conclusion: The thiol/disulphide homeostasis shifted towards oxidative stress, and a decrease in thiols and an increase in disulphides were found in the AA patients. This finding may be responsible for diffuse destruction of hair follicle in the pathogenesis of AA
Anahtar Kelime: Konular:
Alopesi Areata Tanılı Hastalarda Oksidatif Stresin Değerlendirilmesinde Bir Belirteç: Tiyol-Disülfid Homeostazı
Öz: Amaç: Alopesi areata (AA) etiyolojisinde oksidatif stresin rol aldığı, skarsız kıl kaybı ile karakterize bir hastalıktır. AA hastalarında tiyol/disülfid düzeylerine bakarak AA patogenezindeki rolünü ve AA patogenezinde bir belirteç göstergesi olup olmayacağının araştırılması amaçlanmıştır. Gereç ve Yöntemler: Bu kesitsel çalışma 27.07.2016-01.02.2017 tarihleri arasında dermatoloji polikliniğine başvuran AA tanısı alan 100 hasta ve herhangi bir sistemik ve/veya enflamatuvar deri hastalığı olmayan 100 gönüllü kontrol grubunda gerçekleştirildi. Kontrol ve hasta grubu arasında nativ tiyol, total tiyol, disülfid düzeyi, disülfid/nativ tiyol, disülfid/total tiyol ve nativ tiyol/total tiyol oranları karşılaştırıldı. AA hastalarının demografik ve lezyon özellikleri ile nativ tiyol, total tiyol, disülfid düzeyi, disülfid/nativ tiyol, disülfid/total tiyol ve nativ tiyol/total tiyol oranları arasındaki ilişki incelendi. Bulgular: AA’lı hastaların yaş ortalaması 34,9±10,4 yıl, %58’i erkek ve vücut kitle indeksi ortancası 24 kg/m2 olup, kontrol grubu ile yaş ortalaması açısından farklılık saptanmadı (p>0,05). AA hastalarında total tiyol düzeyi, nativ tiyol düzeyi ve nativ tiyol/total tiyol oranında düzeylerinde anlamlı düşüklük saptanırken; disülfid düzeyi, disülfid/nativetiyol ve disülfid/total tiyol oranlarında anlamlı yükseklik saptandı (p<0,05). AA hastalarında lezyonun süresinin, lezyon paterninin, lezyonun genişliğinin ve rekürrens sıklığının; total tiyol, nativ tiyol, disülfid düzeyleri, disülfid/nativ tiyol, disülfid/total tiyol, nativ tiyol/total tiyol oranlarıyla arasında istatistiksel olarak anlamlı bir ilişkiye rastlanmadı (p>0,05). Sonuç: AA hastalarında mevcut mekanizmalardan biri oksidatif stres artışıdır. AA grubunda tiyol/disülfid hemostazı oksidatif stres lehine bozulmuş olup, bu hastalarda tiyollerde azalma, disülfid miktarında artma belirlenmiştir.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Oguz O. Alopesi areata. Turkderm 2014; 48: 40-4.
- Heidarloo KA, Adışen E. Oxidative Stress in Alopecia Areata and its Association with Disease Severity, Treatment and Autoimmunity. Turkiye Klinikleri J Med Sci 2015; 35: 268-78.
- Gilhar A, Etzioni A, Paus R. Alopecia areata. N Engl J Med 2012; 366: 1515-25.
- Alkhalifah A. Alopecia areata update. Dermatol Clin 2013; 31: 93-108.
- Gümüşyayla S, Vural G, Bektaş H, Neşelioğlu S, Deniz O, Erel Ö. Evaluation of Dynamic Thiol-Disulphide Homeostasis in Patients with Epilepsy. Epilepsi 2016; 22: 86-92.
- . Erel O, Neselioglu S. A novel and automated assay for thiol/ disulphide homeostasis. Clin Biochem 2014; 47: 326-32.
- Atmaca G. Antioxidant effects of sulfur-containing amino acids. Yonsei Med J 2004; 45: 776-88.
- Akbas A, Kilinc F, Sener S, Aktaş A, Baran P, Ergin M. Investigation of thiol-disulphide balance in patients with acute urticaria and chronic spontaneous urticaria. Cutan Ocul Toxicol 2017; 36: 205210.
- Turell L, Radi R, Alvarez B. The thiol pool in human plasma: the central contribution of albumin to redox processes. Free Radic Biol Med 2013; 65: 244-53.
- Cremers CM, Jakob U. Oxidant sensing by reversible disulfide bond formation. J Biol Chem 2013; 288: 26489-96.
- Güleç AT, Tanriverdi N, Dürü C, Saray Y, Akçali C. The role of psychological factors in alopecia areata and the impact of the disease on the quality of life. Int J Dermatol 2004; 43: 352-6.
- Goh C, Finkel M, Christos PJ, Sinha AA. Profile of 513 patients with alopecia areata: associations of disease subtypes with atopy, autoimmune disease and positive family history. J Eur Acad Dermatol Venereol 2006; 20: 1055-60.
- Abdel Fattah N, Ebrahim AA, El Okda ES. Lipid peroxidation/ antioxidant activity in patients with alopecia areata. J Eur Acad Dermatol Venereol 2011; 25: 403-8.
- Güngör Ş, Akbay G, Öğüş E, Ekşioglu M, Yücel D. Changes of lipid peroxidation and antioxidant system in serum and tissues of patients with alopecia areata. Turkiye Klinikleri J Dermatol 2008; 18: 141-5.
- Kalkan G, Seçkin HY, Benli İ, Akbaş A, Baş Y, Karakus N, et al. Relationship between manganese superoxide dismutase (MnSODAla-9Val) and glutathione peroxidase (GPx1 Pro 197 Leu) gene polymorphisms and alopecia areata. Int J Clin Exp Med 2015; 8: 21533-40.
- Kökçam I, Naziroğlu M. Antioxidants and lipid peroxidation status in the blood of patients with psoriasis. Clin Chim Acta 1999; 289: 23-31.
- Koca R, Armutcu F, Altinyazar HC, Gürel A. Evaluation of lipid peroxidation, oxidant/antioxidant status, and serum nitric oxide levels in alopecia areata. Medical Sci Mon 2005; 11: 296-9.
- Akar A, Arca E, Erbil H, Akay C, Sayal A, Gür AR. Antioxidant enzymes and lipid peroxidation in the scalp of patients with alopecia areata. J Dermatol Sci 2002; 29: 85-90.
- Yenin JZ, Serarslan G, Yönden Z, Ulutaş KT. Investigation of oxidative stress in patients with alopecia areata and its relationship with disease severity, duration, recurrence and pattern. Clin Exp Dermatol 2015; 40: 617-21.
- Öztürk P, Arıcan O, Kurutaş EB, Mülayim K. Oxidative Stress Biomarkers and Adenosine Deaminase over the Alopecic Area of the Patients with Alopecia Areata. Balkan Med J 2016; 33: 18892.
- Kilinc F, Sener S, Akbas A, Neselioglu S, Erel O, Aktas A. Investigation of Dynamic Thiol-Disulfide Homeostasis in Alopecia Areata Patients. Br J Med Medical Res 2017; 21: 1-7.
- Vandevelde C, Allaerts W. Trichilemmal keratinisation: a causal factor in loosening the murine telogen club hair from the trichilemmal sac. J Anat 1984; 138: 745-56.
- Askelöf P, Axelsson K, Eriksson S, Mannervik B. Mechanism of action of enzymes catalyzing thiol-disulfide interchange. Thioltransferases rather than transhydrogenases. FEBS Lett 1974; 38: 263-7.
- Stenn KS, Paus R. Controls of hair follicle cycling. Physiol Rev 2001; 81: 449-94.
- Kim SW, Kim BJ, Youn SW, Park KC, Huh CH. Evaluation of free oxygen radical and antioxidant capacity in alopecia areata. J Dermatol 2010; 37: 762-4.
- Özcan O, Erdal H, Çakırca G, Yönden Z. Oxidative stress and its impacts on intracellular lipids, proteins and DNA. Journal of Clinical and Experimental Investigations 2015; 6: 331-6.
- Yin H, Xu L, Porter NA. Free radical lipid peroxidation: mechanisms and analysis. Chem Rev 2011; 111: 5944-72.
- Jacob C. Redox signalling via the cellular thiolstat. Biochem Soc Trans 2011; 39: 1247-53.
- De la Fuente M. Effects of antioxidants on immune system ageing. Eur J Clin Nutr 2002; 56(Suppl 3): 5-8
| APA | DEMİR PEKTAŞ S, ALATAŞ E, DOĞAN G, NEŞELİOĞLU S, EREL O (2018). A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. , 205 - 210. 10.4274/meandros.48303 |
| Chicago | DEMİR PEKTAŞ SUZAN,ALATAŞ EMİNE TUĞBA,DOĞAN Gürsoy,NEŞELİOĞLU Salim,EREL Ozcan A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. (2018): 205 - 210. 10.4274/meandros.48303 |
| MLA | DEMİR PEKTAŞ SUZAN,ALATAŞ EMİNE TUĞBA,DOĞAN Gürsoy,NEŞELİOĞLU Salim,EREL Ozcan A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. , 2018, ss.205 - 210. 10.4274/meandros.48303 |
| AMA | DEMİR PEKTAŞ S,ALATAŞ E,DOĞAN G,NEŞELİOĞLU S,EREL O A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. . 2018; 205 - 210. 10.4274/meandros.48303 |
| Vancouver | DEMİR PEKTAŞ S,ALATAŞ E,DOĞAN G,NEŞELİOĞLU S,EREL O A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. . 2018; 205 - 210. 10.4274/meandros.48303 |
| IEEE | DEMİR PEKTAŞ S,ALATAŞ E,DOĞAN G,NEŞELİOĞLU S,EREL O "A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis." , ss.205 - 210, 2018. 10.4274/meandros.48303 |
| ISNAD | DEMİR PEKTAŞ, SUZAN vd. "A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis". (2018), 205-210. https://doi.org/10.4274/meandros.48303 |
| APA | DEMİR PEKTAŞ S, ALATAŞ E, DOĞAN G, NEŞELİOĞLU S, EREL O (2018). A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. Meandros Medical And Dental Journal, 19(3), 205 - 210. 10.4274/meandros.48303 |
| Chicago | DEMİR PEKTAŞ SUZAN,ALATAŞ EMİNE TUĞBA,DOĞAN Gürsoy,NEŞELİOĞLU Salim,EREL Ozcan A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. Meandros Medical And Dental Journal 19, no.3 (2018): 205 - 210. 10.4274/meandros.48303 |
| MLA | DEMİR PEKTAŞ SUZAN,ALATAŞ EMİNE TUĞBA,DOĞAN Gürsoy,NEŞELİOĞLU Salim,EREL Ozcan A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. Meandros Medical And Dental Journal, vol.19, no.3, 2018, ss.205 - 210. 10.4274/meandros.48303 |
| AMA | DEMİR PEKTAŞ S,ALATAŞ E,DOĞAN G,NEŞELİOĞLU S,EREL O A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. Meandros Medical And Dental Journal. 2018; 19(3): 205 - 210. 10.4274/meandros.48303 |
| Vancouver | DEMİR PEKTAŞ S,ALATAŞ E,DOĞAN G,NEŞELİOĞLU S,EREL O A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis. Meandros Medical And Dental Journal. 2018; 19(3): 205 - 210. 10.4274/meandros.48303 |
| IEEE | DEMİR PEKTAŞ S,ALATAŞ E,DOĞAN G,NEŞELİOĞLU S,EREL O "A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis." Meandros Medical And Dental Journal, 19, ss.205 - 210, 2018. 10.4274/meandros.48303 |
| ISNAD | DEMİR PEKTAŞ, SUZAN vd. "A Marker for Evaluation of Oxidative Stress in Patients with Alopecia Areta: Thiol-Disulphide Homeostasis". Meandros Medical And Dental Journal 19/3 (2018), 205-210. https://doi.org/10.4274/meandros.48303 |
