The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells
Yıl: 2019 Cilt: 7 Sayı: 2 Sayfa Aralığı: 80 - 85 Metin Dili: İngilizce DOI: 10.14235/bas.galenos.2018.2287 İndeks Tarihi: 19-02-2020
The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells
Öz: Objective: Green tea (Camellia sinensis) is one of the most consumed beverages in the world. C. sinensis consumption may prevent tumor and other diseases. Insulinomas are pancreatic islet cell tumors. In present study, the effects of the extract from C. sinensis were investigated in rat insulinoma INS-1 cells.Methods: C. sinensis leaves were collected in Rize, Turkey. The leaves were brewed in tap water for 20 and 40 minutes at 80°C. INS-1 cells were treated with different doses (1-5 mg/mL) of C. sinensis extract for 24 h. Cell viability, proliferation, death were examined. The malondialdehyde, glutathione and protein carbonyl levels were measured in INS-1 cells given C. sinensis extract.Results: The cell viability and the percentage of proliferating cell nuclear antigen immunopositive cells decreased, while the percentage of TUNEL positive cells increased in INS-1 cells treated with the extract from C. sinensis according to control group. While malondialdehyde and protein carbonyl levels in INS-1 cells treated with C. sinensis extract decreased, glutathione levels increased according to control group.Conclusion: The results indicate that the extracts from C. sinensis inhibited proliferation and caused apoptosis in INS-1 cell. C. sinensis may be natural agent for supporting the treatment of pancreatic tumors
Anahtar Kelime: Konular:
İnsülinoma INS-1 Hücrelerinde Hücre Çoğalması, Apoptoz ve Oksidatif Stres Üzerine Camellia sinensis Ekstraktının Etkileri
Öz: Amaç: Yeşil çay (Camellia sinensis) dünyanın en çok tüketilen içeceklerinden biridir. C. sinensis tüketimi tümör ve diğer hastalıkları önleyebilir. İnsülinomlar pankreatik ada hücre tümörleridir. Bu çalışmada, sıçan insülinoma INS-1 hücrelerinde C. sinensis ekstraktının etkileri araştırılmıştır. Yöntemler: C. sinensis yaprakları Türkiye’de Rize’de toplandı. Yapraklar 80°C’de 20 ve 40 dakika musluk suyunda demlendi. INS-1 hücreleri, 24 saat boyunca C. sinensis ekstraktının farklı dozları (1-5 mg/mL) ile muamele edildi. Hücre canlılığı, çoğalması, ölümü incelendi. C. sinensis ekstraktı verilen INS-1 hücrelerinde malondialdehit, glutatyon ve protein karbonil seviyeleri ölçüldü. Bulgular: C. sinensis ekstraktı ile muamele edilen INS-1 hücrelerinde kontrol grubuna göre hücre canlılığı ve PCNA immünpozitif hücrelerin yüzdesi azalırken, TUNEL pozitif hücrelerin yüzdesi artmıştır. C. sinensis ekstraktı ile muamele edilen INS-1 hücrelerinde kontrol grubuna göre malondialdehit ve protein karbonil seviyeleri azalırken, glutatyon seviyeleri artmıştır. Sonuç: Sonuçlar INS-1 hücresinde C. sinensis ektraktının çoğalmayı inhibe ettiğini ve apoptoza neden olduğunu göstermektedir. C. sinensis, pankreas tümörlerinin tedavisini destekleyen doğal bir ajan olabilir.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | KAÇAR A, Ersöz M, sezekler I, COŞKUN Z (2019). The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. , 80 - 85. 10.14235/bas.galenos.2018.2287 |
| Chicago | KAÇAR Ayşe Karatuğ,Ersöz Melike,sezekler Isil,COŞKUN Zeynep Mine The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. (2019): 80 - 85. 10.14235/bas.galenos.2018.2287 |
| MLA | KAÇAR Ayşe Karatuğ,Ersöz Melike,sezekler Isil,COŞKUN Zeynep Mine The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. , 2019, ss.80 - 85. 10.14235/bas.galenos.2018.2287 |
| AMA | KAÇAR A,Ersöz M,sezekler I,COŞKUN Z The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. . 2019; 80 - 85. 10.14235/bas.galenos.2018.2287 |
| Vancouver | KAÇAR A,Ersöz M,sezekler I,COŞKUN Z The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. . 2019; 80 - 85. 10.14235/bas.galenos.2018.2287 |
| IEEE | KAÇAR A,Ersöz M,sezekler I,COŞKUN Z "The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells." , ss.80 - 85, 2019. 10.14235/bas.galenos.2018.2287 |
| ISNAD | KAÇAR, Ayşe Karatuğ vd. "The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells". (2019), 80-85. https://doi.org/10.14235/bas.galenos.2018.2287 |
| APA | KAÇAR A, Ersöz M, sezekler I, COŞKUN Z (2019). The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. Bezmiâlem Science, 7(2), 80 - 85. 10.14235/bas.galenos.2018.2287 |
| Chicago | KAÇAR Ayşe Karatuğ,Ersöz Melike,sezekler Isil,COŞKUN Zeynep Mine The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. Bezmiâlem Science 7, no.2 (2019): 80 - 85. 10.14235/bas.galenos.2018.2287 |
| MLA | KAÇAR Ayşe Karatuğ,Ersöz Melike,sezekler Isil,COŞKUN Zeynep Mine The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. Bezmiâlem Science, vol.7, no.2, 2019, ss.80 - 85. 10.14235/bas.galenos.2018.2287 |
| AMA | KAÇAR A,Ersöz M,sezekler I,COŞKUN Z The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. Bezmiâlem Science. 2019; 7(2): 80 - 85. 10.14235/bas.galenos.2018.2287 |
| Vancouver | KAÇAR A,Ersöz M,sezekler I,COŞKUN Z The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells. Bezmiâlem Science. 2019; 7(2): 80 - 85. 10.14235/bas.galenos.2018.2287 |
| IEEE | KAÇAR A,Ersöz M,sezekler I,COŞKUN Z "The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells." Bezmiâlem Science, 7, ss.80 - 85, 2019. 10.14235/bas.galenos.2018.2287 |
| ISNAD | KAÇAR, Ayşe Karatuğ vd. "The Effects of Camellia sinensis Extract on Proliferation, Apoptosis and Oxidative Stress in Insulinoma INS-1 Cells". Bezmiâlem Science 7/2 (2019), 80-85. https://doi.org/10.14235/bas.galenos.2018.2287 |
