Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients

Buyuk, Melek; Karabulut, Nuran; Alacam, Sema; AĞAÇFİDAN, Ali; ÖNEL, Mustafa; Gulluoglu, Mine; YOLCU, Ayfer

doi:10.4274/vhd.galenos.2018-0018

Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients

Ayfer YOLCU, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Nuran KARABULUT, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Sema ALAÇAM, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Mustafa ÖNEL, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Melek BÜYÜK, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

Mine GÜLLÜOĞLU, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Patoloji Anabilim Dalı, İstanbul, Türkiye)

Ali AĞAÇFİDAN (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, İstanbul, Türkiye)

Viral Hepatitis Journal

14 3

Yıl: 2019 Cilt: 25 Sayı: 1 Sayfa Aralığı: 14 - 18 Metin Dili: İngilizce DOI: 10.4274/vhd.galenos.2018-0018 İndeks Tarihi: 10-06-2020

Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients

Öz:

Objectives: The prevalence of hepatitis delta virus (HDV) worldwide shows geographical differences. Although there are several studies on anti-HDV seroprevalence rates in hepatitis B surface antigen (HBsAg)-positive patients in Turkey, studies on HDV-RNA prevalence in this patient population are limited. It was aimed to detect the frequency of anti-HDV antibodies and HDV-RNA in HBsAg-positive patients in this study. Materials and Methods: This retrospective study included 2089 HBsAg-positive patients in whom anti-HDV was analyzed between April 2015 and March 2017. Anti-HDV test was performed in serum samples obtained from HBsAg-positive patients by enzyme-linked immunosorbent assay. In anti-HDV-positive patients, HDV-RNA was analyzed in serum samples by real-time polymerase chain reaction. Results: The seroprevalence of anti-HDV was 4.1% (85/2089), while the rate of HDV-RNA positivity was 2.4% (51/2089). HDVRNA was detected in 60% (51/85) of anti-HDV-positive patients. The frequency of anti-HDV and HDV-RNA was highest in the 50-59 age group. Conclusion: The frequency of HDV in this study was found to be consistent with regional data. HDV viremia was detected in only 60% (51/85) of the anti-HDV-positive patients. Since anti-HDV antibodies may remain after recovery, it is important to investigate HDV-RNA to determine the true prevalence of HDV

Anahtar Kelime:

Hepatit B Yüzey Antijeni-pozitif Hastalarda Hepatit Delta Virüsünün Sıklığı

Öz:

Amaç: Dünya genelinde hepatit delta virüsünün (HDV) yaygınlığı coğrafi farklılıklar göstermektedir. Her ne kadar Türkiye’de hepatit B yüzey antijeni (HBsAg) pozitif olan hastalarda anti-HDV seroprevalans oranları konusunda birçok çalışma olmasına rağmen, bu hasta popülasyonunda HDV-RNA prevalansı ile ilgili çalışmalar sınırlıdır. Bu çalışmada HBsAg pozitif olan hastalarda anti-HDV antikorları ve HDVRNA sıklığının saptanması amaçlandı. Gereç ve Yöntemler: Bu retrospektif çalışma, Nisan 2015 ve Mart 2017 tarihleri arasında HBsAg-pozitif hastalarda anti-HDV testi çalışılan 2089 hastayı kapsadı. HBsAg-pozitif hastalardan alınan serum örneklerinde anti-HDV testi, enzim bağlı immünosorbent metod kullanılarak yapıldı. Anti-HDV-pozitif hastalarda, HDV-RNA, gerçek zamanlı polimeraz zincir reaksiyonu ile serum örneklerinde analiz edildi. Bulgular: Anti-HDV seroprevalansı %4,1 (85/2089) iken HDV-RNA oranı %2,4 (51/2089) idi. HDV-RNA, anti-HDV pozitif olan hastaların %60’ında (51/85) tespit edildi. Anti-HDV ve HDV-RNA sıklığı 50-59 yaş grubunda en yüksekti. Sonuç: Bu çalışmada HDV sıklığı bölgesel verilerle tutarlı bulundu. HDV viremisi, anti-HDV pozitif olan hastaların sadece %60’ında (51/85) tespit edildi. Anti-HDV antikorları iyileşmeden sonra da pozitif kalabileceğinden dolayı, HDV’nin gerçek prevalansını belirlemek için HDV-RNA’nın araştırılması önemlidir.

Anahtar Kelime:

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Sultanik P, Pol S. Hepatitis delta virus: Epidemiology, natural course and treatment. J Infect Dis Ther. 2016;4:271.
2. Botelho-Souza LF, Vasconcelos MPA, dos Santos AO, Salcedo JMV, Vieira DS. Hepatitis delta: virological and clinical aspects. Virol J. 2017;14:177.
3. ICTV. 9th Report (2011). Virus Taxonomy: 2017 Release. Delta virus (08.03.2018). Availablefrom: https://talk.ictvonline.org/ ictv-reports/ictv_9th_report/negative-sense-rna-viruses-2011/w/ negrna_viruses/211/deltavirus.
4. Tahaei SM, Mohebbi SR, Azimzadeh P, Behelgardi A, Sanati A, Mohammadi P, Khanyaghma M, Hosseini Razavi A, Sharifian A, Zali MR. Prevalence of hepatitis D virus in hepatitis B virus infected patients referred to Taleghani hospital, Tehran, Iran. Gastroenterol Hepatol Bed Bench. 2014;7:144-150.
5. Doğan M, Güneş H, Mete R, Taş T, Mengeloğlu FZ, Küçükbayrak A. Prevalence of anti-HDV and HDAg in patients with chronic hepatitis B. Dicle Med J. 2013;40:50-53.
6. Celen MK, Kandemir O. Hepatit delta virüsü enfeksiyonunun epidemiyolojisi. İçinde: Kandemir O, Danalıoglu A, editörler. Hepatit B’den D’ye hepatit güncel klinik el kitabı. Istanbul: Viral Hepatitle Savaşım Derneği; 2015;p.267-271.
7. Kemal Celen M, Tekin Koruk S, Aygen B, Dal T, Karabay O, Tosun S, Koksal I, Turgut H, Onlen Y, Balik I, Yildirim N, Sinan Dal M, Ayaz C, Tabak F. The characteristics of patients with chronic hepatitis B in Turkey. Med Glas (Zenica). 2014;11:94-98.
8. Yalcın K, Tuncel ET, Gunduz F. Latest updates on chronic delta hepatitis. Marmara Medical Journal. 2016;29:50-54.
9. Noureddin M, Gish R. Hepatitis delta: epidemiology, diagnosis and management 36 years after discovery. Curr Gastroenterol Rep. 2014;16:365.
10. Olivero A, Smedile A. Hepatitis delta virus diagnosis. Semin liver dis. 2012;220-227.
11. WHO. Hepatitis D Fact Sheet (13.10.2017). Available from: http:// www.who.int/mediacentre/factsheets/hepatitis-d/en/.
12. Iskender G, Ogan MC, Sayılır K, Dirim EB, Batı S, Cimentepe M, Yenigun A. Seroprevalerıce of anti-HDV antibody in HBsAg positive patients. Acta Oncologica Turcica. 2006;39:99-100.
13. Uzun B, Şener AG, Güngör S, Afşar I, Demirci M. Evaluation of hepatitis delta virus (HDV) infection in blood donors in western Turkey. Transfus Apher Sci. 2014;50:388-391.
14. Parlak E, Erturk A, Parlak M, Kosan Z, Albayrak A, Ozkurt Z, Ozden K, Erol S. Assessment of patients with hepatitis D. Viral Hepat J. 2015;21:80-84.
15. Kurtoglu MG, Ustun C, Bozkurt H, Tuncer O, Berktas M. Hepatitis D virus seroprevalence determined during periods of hepatitis B virus infections In Eastern Turkey. Viral Hepat J. 2009;14:27-32.
16. Kose S, Ece G, Gozaydin A, Turken M. Study on seroprevalence of hepatitis delta in a regional hospital in Western Turkey. J Infect Dev Ctries. 2012;6:782-785.
17. Korkmaz P, Aykın N, Cevik FC, Guduren HM, Alpay Y. Seropositivity of delta hepatitis in HBsAg positive patients in Eskişehir province. Viral Hepat J. 2014;20:72-74.
18. Karadag A, Yılmaz H, Goren I, Acuner IC, Eroglu C, Gunaydın M. Defining the delta virus positivity in hepatitis B virus infections. Viral Hepat J. 2014;20:64-66.
19. Inci A, Fincanci M, Muderrisoglu C. Investigation of anti-hepatitis delta virus and anti-hepatitis C virus in patients with hepatitis B virus infection/kronik hepatit B’li olgularda anti hepatit delta virüs (anti HDV), anti hepatit C virus (anti-HCV) antikorları sıklığının araştırılması. Istanbul Med J. 2013;14:109-112.
20. Gurkan Y, Toyran A, Aksoy A, Coskun FA, Cetin F. Evaluation of HBsAg and anti-HDV seroprevalance of patients who admitted to Ankara Numune Training and Research Hospital between 20102013. Viral Hepatitis J. 2013;19:148-151.
21. Duman Y, Tekerekoglu MS, Ay S. Seroprevalence of HBsAg, antiHBs, anti-HDV and HDVAg in İnönü University Medical Faculty Hospital, 2012. Med-Science. 2014;3:982-990.
22. Dulger AC, Suvak B, Gonullu H, Gonullu E, Gultepe B, Aydın İ, Batur A, Karadas S, Olmez Ş. High prevalence of chronic hepatitis D virus infection in Eastern Turkey: urbanization of the disease. Arch Med Sci. 2016;12:415-420.
23. Doğan M, Güneş H, Mete R, Taş T, Mengeloğlu FZ, Küçükbayrak A. Prevalence of anti-HDV and HDAg in patients with chronic hepatitis B. Dicle Med J. 2013;40:50-53.
24. Demirdal T, Demirtürk N, Aşcı Z. Afyonkarahisar ilinde hepatit delta virüsü seroprevalansı. Viral Hepatit Derg. 2009;14:104-107.
25. Kandemir Ö, Ersöz G, Uğuz K, Kaya A. Kronik hepatit B infeksiyonlu hastalarda anti-HDV sıklığı. Viral Hepatit Derg. 2001;1:263-265.
26. Kölgelier S, Demir NA, Özçimen S. Seropositivity of delta hepatitis in HBsAg positive patients in Adıyaman province. Viral Hep J. 2013;19:8-10.
27. Mese S, Nergiz S, Tekes S, Gul K. Seroprevalence of serum HBsAg positivity and hepatitis delta virus infection among blood donors in Southeastern Turkey. Clin Ter. 2014;165:95-98.
28. Bahcecioglu I, Aygun C, Gozel N, Poyrazoglu O, Bulut Y, Yalniz M. Prevalence of hepatitis delta virus (HDV) infection in chronic hepatitis B patients in eastern Turkey: still a serious problem to consider. J Viral Hep. 2011;18:518-524.
29. Popescu GA, Otelea D, Gavriliu LC, Neaga E, Popescu C, Paraschiv S, Fratila M. Epidemiology of hepatitis D in patients infected with hepatitis B virus in bucharest: A cross‐sectional study. J Med Virol. 2013;85:769-774.
30. Gheorghe L, Csiki IE, Iacob S, Gheorghe C, Trifan A, Grigorescu M, Motoc A, Suceveanu A, Curescu M, Caruntu F, Sporea I, Brisc C, Rogoveanu I, Cerban R, Tugui L, Alexandrescu A. Hepatitis delta virus infection in Romania: prevalence and risk factors. J Gastrointestin Liver Dis. 2015;24:413-421.
31. Jamjoom GA, El-Daly MM, Azhar EI, Fallatah HI, Akbar HO, Babatin M, Alghamdi SA, Dgdgi MI, Hamid MA, Qari YA, El-Kafrawy SA. Prevalence and molecular characterization of hepatitis D virus in Saudi Arabia: A single-center study. Saudi J Gastroenterol. 2017;23:176-182.
32. Sagnelli E, Sagnelli C, Pisaturo M, Macera M, Coppola N. Epidemiology of acute and chronic hepatitis B and delta over the last 5 decades in Italy. World J Gastroenterol. 2014;20:7635-7643.
33. Hughes SA, Wedemeyer H, Harrison PM. Hepatitis delta virus. Lancet. 2011;378:73-85.
34. Gomaa N, Metwally LA, Nemr N, Younis S. Seroprevalence of HDV infection in HBsAg positive population in Ismailia, Egypt. Egypt J Immunol. 2013;20:23-28.
35. Değertekin H, Yalçın K, Yakut M, Yurdaydin C. Seropositivity for delta hepatitis in patients with chronic hepatitis B and liver cirrhosis in Turkey: a meta-analysis. Liver Int. 2008;28:494-498.
36. Yozgat A, Altiparmak E, Demirci S, Caglayan O, Aliyazicioglu M, Koseoglu HT, Erten AT, Cetin F, Uner E, Ozaslan E, Altınbas A. Delta hepatitis frequency in chronic hepatitis B patients: Single center retrospective. Ortadogu Medical Journal. 2015;7:12-15.
37. Cross TJ, Rizzi P, Horner M, Jolly A, Hussain MJ, Smith HM, Vergani D, Harrison PM. The increasing prevalence of hepatitis delta virus (HDV) infection in South London. J Med Virol. 2008;80:277-282.
38. Seetlani NK, Abbas Z, Raza S, Yakoob J, Jafri W. Prevalence of hepatitis D in HBsAg positive patients visiting liver clinics. J Pak Med Assoc. 2009;59:434-437.

APA	YOLCU A, Karabulut N, Alacam S, ÖNEL M, Buyuk M, Gulluoglu M, AĞAÇFİDAN A (2019). Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. , 14 - 18. 10.4274/vhd.galenos.2018-0018
Chicago	YOLCU Ayfer,Karabulut Nuran,Alacam Sema,ÖNEL Mustafa,Buyuk Melek,Gulluoglu Mine,AĞAÇFİDAN Ali Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. (2019): 14 - 18. 10.4274/vhd.galenos.2018-0018
MLA	YOLCU Ayfer,Karabulut Nuran,Alacam Sema,ÖNEL Mustafa,Buyuk Melek,Gulluoglu Mine,AĞAÇFİDAN Ali Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. , 2019, ss.14 - 18. 10.4274/vhd.galenos.2018-0018
AMA	YOLCU A,Karabulut N,Alacam S,ÖNEL M,Buyuk M,Gulluoglu M,AĞAÇFİDAN A Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. . 2019; 14 - 18. 10.4274/vhd.galenos.2018-0018
Vancouver	YOLCU A,Karabulut N,Alacam S,ÖNEL M,Buyuk M,Gulluoglu M,AĞAÇFİDAN A Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. . 2019; 14 - 18. 10.4274/vhd.galenos.2018-0018
IEEE	YOLCU A,Karabulut N,Alacam S,ÖNEL M,Buyuk M,Gulluoglu M,AĞAÇFİDAN A "Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients." , ss.14 - 18, 2019. 10.4274/vhd.galenos.2018-0018
ISNAD	YOLCU, Ayfer vd. "Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients". (2019), 14-18. https://doi.org/10.4274/vhd.galenos.2018-0018

APA	YOLCU A, Karabulut N, Alacam S, ÖNEL M, Buyuk M, Gulluoglu M, AĞAÇFİDAN A (2019). Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. Viral Hepatitis Journal, 25(1), 14 - 18. 10.4274/vhd.galenos.2018-0018
Chicago	YOLCU Ayfer,Karabulut Nuran,Alacam Sema,ÖNEL Mustafa,Buyuk Melek,Gulluoglu Mine,AĞAÇFİDAN Ali Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. Viral Hepatitis Journal 25, no.1 (2019): 14 - 18. 10.4274/vhd.galenos.2018-0018
MLA	YOLCU Ayfer,Karabulut Nuran,Alacam Sema,ÖNEL Mustafa,Buyuk Melek,Gulluoglu Mine,AĞAÇFİDAN Ali Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. Viral Hepatitis Journal, vol.25, no.1, 2019, ss.14 - 18. 10.4274/vhd.galenos.2018-0018
AMA	YOLCU A,Karabulut N,Alacam S,ÖNEL M,Buyuk M,Gulluoglu M,AĞAÇFİDAN A Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. Viral Hepatitis Journal. 2019; 25(1): 14 - 18. 10.4274/vhd.galenos.2018-0018
Vancouver	YOLCU A,Karabulut N,Alacam S,ÖNEL M,Buyuk M,Gulluoglu M,AĞAÇFİDAN A Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients. Viral Hepatitis Journal. 2019; 25(1): 14 - 18. 10.4274/vhd.galenos.2018-0018
IEEE	YOLCU A,Karabulut N,Alacam S,ÖNEL M,Buyuk M,Gulluoglu M,AĞAÇFİDAN A "Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients." Viral Hepatitis Journal, 25, ss.14 - 18, 2019. 10.4274/vhd.galenos.2018-0018
ISNAD	YOLCU, Ayfer vd. "Frequency of Hepatitis Delta Virus in Hepatitis B Surfaceantigen-positive Patients". Viral Hepatitis Journal 25/1 (2019), 14-18. https://doi.org/10.4274/vhd.galenos.2018-0018