The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines
Yıl: 2019 Cilt: 16 Sayı: 3 Sayfa Aralığı: 273 - 281 Metin Dili: İngilizce DOI: 10.4274/tjps.galenos.2018.27146 İndeks Tarihi: 25-08-2020
The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines
Öz: Objectives: Natural products originating from plants have been used for many years in the treatment of various diseases, including cancer.Centaurea solstitialis subsp. solstitialis is used in Turkish folk medicine. This study was the first to determine the in vitro biological effects ofethanolic extract from the flowering parts of C. solstitialis L. subsp. solstitialis collected from Muğla Province.Materials and Methods: The cytotoxic effect was evaluated against Daudi, A549, and HeLa cancer cells and one normal BEAS-2B cell line usingthe MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- dipenyltetrazolium bromide) assay. Flow cytometric analysis and the caspase-3 activity assay wereperformed to detect apoptotic cell death. Angiogenic factor [vascular endothelial growth factor (VEGF)] secretion and the release of interleukin(IL)-1α, IL-6, and tumor necrosis factor (TNF)-α by cells treated with the extract were measured using enzyme-linked immunosorbent assay.Results: The extract exhibited cytotoxic effects against all the cancer cell lines used but HeLa and Daudi were the most sensitive cells, with IC50values of 63.18 µg/mL and 69.27 µg/mL, respectively. Selective cytotoxicity was observed between the HeLa and normal BEAS-2B cell lines. Theextract arrested the cell cycle at the S and G2 phases. In addition, apoptotic cell death was detected in HeLa and A549 cells. Moreover, the plantextract caused a significant decrease in VEGF secretion in A549 cells and a fluctuation in IL-1α, IL-6, and TNF-α secretion in A549 and Daudi cells.Conclusion: These observations suggest that the flowering parts of C. solstitialis may be a potential source in the development of natural drugs forthe treatment of cancer and modulation of cytokine secretion.
Anahtar Kelime: İnsan Kanser Hücre Hatları Üzerinde Centaurea solstitialis Özütünün AntiKanser ve Anti-İnflamatuar Etkileri
Öz: Amaç: Bitkilerden elde edilen doğal ürünler, kanser dahil çeşitli hastalıkların tedavisinde uzun yıllardır kullanılmaktadır. Centaurea solstitialis subsp. solstitialis’in Türk geleneksel tıbbında yeri olduğu bilinmektedir. Bu araştırma, Muğla ilinden toplanan C. solstitialis’in çiçekli kısımlarından elde edilen etanolik özütün in vitro biyolojik etkilerini belirleyen ilk çalışmadır. Gereç ve Yöntemler: Özütün Daudi, A549 ve HeLa kanser hücrelerine ve normal BEAS-2B hücre hattına karşı sitotoksik etkisi MTT (3-(4,5- dimetiltiyazol-2-il)-2,5-dipeniltetrazolyum bromür) testi ile belirlendi. Apoptotik hücre ölümü akış sitometri analizi ve kaspaz-3 aktivite deneyleriyle araştırıldı. Özüt ile muamele edilen hücreler tarafından üretilen anjiyojenik faktör [vasküler endotel büyüme faktörü (VEGF)] salınımı ve sitokinlerden interlökin (IL)-lα, IL-6 ve tümör nekroz faktör (TNF)-α’nın salınımı enzim bağlı immünosorbent deneyi testleriyle ölçüldü. Bulgular: Bitki özütü kullanılan tüm kanser hücre hatlarına karşı sitotoksik etki gösterirken, özüte karşı en duyarlı hücrelerin 63.18 µg/mL and 69.27 µg/mL olan IC50 değerleri ile sırasıyla HeLa ve Daudi hücreleri olduğu gözlendi. Seçici sitotoksisite HeLa ve normal BEAS-2B hücre hatları arasında tespit edildi. Bitki özütü S ve G2 fazlarında hücre döngüsü arrestine yol açtı. Buna ilave olarak, HeLa ve A549 hücrelerinde apoptotik hücre ölümü kaydedildi. Ayrıca bitki özütü A549 hücrelerinin VEGF salgılamasında anlamlı bir düşüşe yol açarken, A549 ile Daudi hücrelerinin IL-lα, IL-6 ve TNF-α salgılamasında önemli değişime neden oldu. Sonuç: Bu bulgular kanser tedavisi ve sitokin salgısının modülasyonunda gerekli olan doğal ilaçların geliştirilmesi için C. solstitialis’in çiçekli kısımlarının potansiyel bir kaynak olabileceğini göstermektedir.
Anahtar Kelime: Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | Alper M, güneş h (2019). The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. , 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| Chicago | Alper Mehlika,güneş hatice The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. (2019): 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| MLA | Alper Mehlika,güneş hatice The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. , 2019, ss.273 - 281. 10.4274/tjps.galenos.2018.27146 |
| AMA | Alper M,güneş h The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. . 2019; 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| Vancouver | Alper M,güneş h The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. . 2019; 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| IEEE | Alper M,güneş h "The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines." , ss.273 - 281, 2019. 10.4274/tjps.galenos.2018.27146 |
| ISNAD | Alper, Mehlika - güneş, hatice. "The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines". (2019), 273-281. https://doi.org/10.4274/tjps.galenos.2018.27146 |
| APA | Alper M, güneş h (2019). The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. Turkish Journal of Pharmaceutical Sciences, 16(3), 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| Chicago | Alper Mehlika,güneş hatice The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. Turkish Journal of Pharmaceutical Sciences 16, no.3 (2019): 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| MLA | Alper Mehlika,güneş hatice The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. Turkish Journal of Pharmaceutical Sciences, vol.16, no.3, 2019, ss.273 - 281. 10.4274/tjps.galenos.2018.27146 |
| AMA | Alper M,güneş h The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. Turkish Journal of Pharmaceutical Sciences. 2019; 16(3): 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| Vancouver | Alper M,güneş h The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines. Turkish Journal of Pharmaceutical Sciences. 2019; 16(3): 273 - 281. 10.4274/tjps.galenos.2018.27146 |
| IEEE | Alper M,güneş h "The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines." Turkish Journal of Pharmaceutical Sciences, 16, ss.273 - 281, 2019. 10.4274/tjps.galenos.2018.27146 |
| ISNAD | Alper, Mehlika - güneş, hatice. "The Anticancer and Anti-inflammatory Effects of Centaurea solstitialis Extract on Human Cancer Cell Lines". Turkish Journal of Pharmaceutical Sciences 16/3 (2019), 273-281. https://doi.org/10.4274/tjps.galenos.2018.27146 |
