The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey

Bekir ÇÖL, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Alper TONGUÇ, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Okan ÖZGÜL, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Hasan Sungur CİVELEK, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
Betül KAYA (Ege Üniversitesi, Mühendislik Fakültesi, İzmir, Türkiye)
Türkiye Entomoloji Dergisi
1 0
Yıl: 2006 Cilt: 30 Sayı: 4 Sayfa Aralığı: 243 - 253 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey

Öz:
Liriomyza trifolii (Burgess, 1880), Liriomyza congesta, (Becker, 1903) Agromyza apfelbecki Strobl, 1902 ve Chromatomyia horticola (Goureau, 1851) türleri Türkiye’nin tarımsal alanlarında önemli sebze zararlılarındandır. Bu türler morfolojik karakterleri ile kolaylıkla ayırt edilemezler. Bu çalışmanın amacı, bu türleri kesin bir şekilde ayırt edebilmek için genetik işaretleyicileri oluşturmak ve Türkiye’deki değişik coğrafik alanlardan toplanan L. trifolii bireyleri arasındaki genetik varyasyonları kavramaktır. Böylece 4 rastgele primer kullanılarak RAPD işaretleyicileri çalıştırılmıştır. Farklı RAPD profilleri farklı türlerin belirlenmesinde kullanılmış ve böylece RAPD-PCR analizlerinin, klasik metotlar yoluyla rutin bir şekilde ele alınarak bu türlerin ayırt edilmesine yardımcı olma doğrultusunda hızlı bir tarama için kullanışlı bir araç olarak başvurulabileceğini göstermiştir. Sebze ürünlerine zarar vererek önemli ekonomik kayıplara neden olduğundan dolayı bu zararlının yayılımını önlemeye yönelik kontrol stratejilerinin geliştirilmesi için bu türlerin biyotiplerinin tanımlanması önemlidir. Bu çalışmadan elde edilen sonuçlar RAPD’in, türlerin ve ırklarının geleneksel teşhislerine önemli bir alternatif olarak umut verici bir şekilde etkili, hızlı ve ekonomik bir yöntem olduğunu, göstermiştir.
Anahtar Kelime: Liriomyza congesta Liriomyza trifolii Agromyza apfelbecki yaprak galeri sineği RAPD DNA tanı sebzeler Agromyzidae bitki zararlıları genetik uzaklık Chromatomyia horticola karakterizasyon

Konular: Biyoloji

Türkiye 'den toplanan Liriomyza trifolii (Burgess,1880), L.congesta (Becker,1903), Agromyza apfelbecki Strobl,1902 ve chromatomyia horticola (Goureau,1851) türlerinin karakterize edilmesinde RAPD-PCR analizinin kullanımı

Öz:
The Liriomyza trifolii (Burgess, 1880), Liriomya congesta (Becker, 1903), Agromyza apfelbecki Strobl, 1902 and Chromatomyia horticola (Goureau, 1851) species have become important vegetable pests in the agricultural areas in Turkey. These species are not easily differentiated by their morphological characteristics. The aim of the present study was to obtain genetic markers to unambiguously distinguish these species and gain insight into genetic variation between the individuals of L. trifolii strains collected from various geographic locations in Turkey. Thus, four random primers were employed to generate RAPD markers. Different RAPD profiles were observed for the different species indicating that the RAPD-PCR analysis can be applied as a useful tool in quickly screening the strains to aid in discriminating these species that was routinely done via classical methods. Identification of biotypes of the species is crucial in order to designing control strategies to avoid the spread of the pests because of economic losses caused by the damages to vegetable crops. Our results show that RAPD is promisingly an effective, fast and economic way, hence proposed as a valuable alternative to traditional identification of the insect species and strains.
Anahtar Kelime: vegetables Agromyzidae plant pests genetic distance Chromatomyia horticola characterization Liriomyza congesta Liriomyza trifolii Agromyza apfelbecki leaf miners RAPD DNA identification

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA ÇÖL B, TONGUÇ A, ÖZGÜL O, CİVELEK H, KAYA B (2006). The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. , 243 - 253.
Chicago ÇÖL Bekir,TONGUÇ Alper,ÖZGÜL Okan,CİVELEK HASAN SUNGUR,KAYA Betül The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. (2006): 243 - 253.
MLA ÇÖL Bekir,TONGUÇ Alper,ÖZGÜL Okan,CİVELEK HASAN SUNGUR,KAYA Betül The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. , 2006, ss.243 - 253.
AMA ÇÖL B,TONGUÇ A,ÖZGÜL O,CİVELEK H,KAYA B The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. . 2006; 243 - 253.
Vancouver ÇÖL B,TONGUÇ A,ÖZGÜL O,CİVELEK H,KAYA B The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. . 2006; 243 - 253.
IEEE ÇÖL B,TONGUÇ A,ÖZGÜL O,CİVELEK H,KAYA B "The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey." , ss.243 - 253, 2006.
ISNAD ÇÖL, Bekir vd. "The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey". (2006), 243-253.
APA ÇÖL B, TONGUÇ A, ÖZGÜL O, CİVELEK H, KAYA B (2006). The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. Türkiye Entomoloji Dergisi, 30(4), 243 - 253.
Chicago ÇÖL Bekir,TONGUÇ Alper,ÖZGÜL Okan,CİVELEK HASAN SUNGUR,KAYA Betül The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. Türkiye Entomoloji Dergisi 30, no.4 (2006): 243 - 253.
MLA ÇÖL Bekir,TONGUÇ Alper,ÖZGÜL Okan,CİVELEK HASAN SUNGUR,KAYA Betül The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. Türkiye Entomoloji Dergisi, vol.30, no.4, 2006, ss.243 - 253.
AMA ÇÖL B,TONGUÇ A,ÖZGÜL O,CİVELEK H,KAYA B The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. Türkiye Entomoloji Dergisi. 2006; 30(4): 243 - 253.
Vancouver ÇÖL B,TONGUÇ A,ÖZGÜL O,CİVELEK H,KAYA B The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey. Türkiye Entomoloji Dergisi. 2006; 30(4): 243 - 253.
IEEE ÇÖL B,TONGUÇ A,ÖZGÜL O,CİVELEK H,KAYA B "The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey." Türkiye Entomoloji Dergisi, 30, ss.243 - 253, 2006.
ISNAD ÇÖL, Bekir vd. "The use of RAPD-PCR analysis in characterization of Liriomyza congesta (Becker 1903), Agromyza apfelbecki strobl,1902 and Chromatomyia horticola (Goureau, 1851) species collected from Turkey". Türkiye Entomoloji Dergisi 30/4 (2006), 243-253.
Sayfa Oluşturulma Tarihi
22-04-2024 12:44

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