Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields

Okmen Gülten KURUCUOĞLU, (Muğla Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Muğla, Türkiye)
GÖNÜL DÖNMEZ, (Ankara Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Ankara, Türkiye)
Sedat DÖNMEZ (Ankara Üniversitesi, Mühendislik Fakültesi, Ankara, Türkiye)
Journal of Applied Biological Sciences
0 0
Yıl: 2007 Cilt: 1 Sayı: 1 Sayfa Aralığı: 57 - 62 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields

Öz:
Samples were collected from paddy fields in Corum-TURKIYE. Nitrogen-free BG-11 medium was used for isolation of nitrogen fixing cyanobacteria. Acetylene reduction technique was used to determine the effects of different chemical agents on the nitrogenase activities of the cyanobacteria, which were identified at the genus level. Nodularia showed the highest nitrogenase activity (0.006 ethylene ul/ mg.h) at lOmM nitrate concentration. At 25mM phosphate concentration, Nodularia showed the highest nitrogenase activity (0.006 ethylene ul/ mg.h ). The highest tolerances for the herbicides were present in Nodularia (0.06 ethylene ul/ mg.h) for bensulphuron (50ug /ml) and Nostoc 6 ethylene ul/ mg.h (for molinate lOOug / ml).
Anahtar Kelime: nitrate enzyme activity phosphate environmental factors nitrogenase cyanobacteria herbicides

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • [1]. Manahan SE. 1997. The Nitrogen Cycle. In: Environmental Science and Technology, Lewis Publishers, N.York, 466-468.
  • [2]. Madigan MT,,Martinko JM and Parker J. 1997,Brock Biology of Microorganisms. Prentice-Hall Int. Ltd. 8th edition. In: Chapter 4, pp. 13,14, and 15, London.
  • [3]. Vignais PM. et al. 1985. Biochemistry of nitrogenase. Advances in Microbial Physiology, 26: 190-234.
  • [4]. Postgate J. 1989 Trends and perspectives in nitrogen fixation research. Advance in Microbial Physiology, 30: 1-21.
  • [5]. Herrero A, Muro-Pastor AM and Flores E. 2001 Nitrogen control in' cyanobacteria. Journal of Bacteriology, 183 (2): 411-425.
  • [6]. Albrecht SL. 1998. Eukaryotic algae and cyanobacteria. In: Principles and Applications of Soil Microbiology, Prentice-Hall, Inc., America, 94-103.
  • [7]. Irisarri P, Gonnet S and Monza J. 2001. Cyanobacteria in Uruguayan rice fields: diversity, nitrogen fixing ability and tolerance to herbicides and combined nitrogen. Journal of Biotechnology, 91 (3): 95-103.
  • [8]. Metting B. 1990. Microalgae applications in agriculture. Developments in Industrial Microbiology, 31:265-270.
  • [9]. Singh S. 1991. Involvement of ammonium assimilation in ammonium inhibition of nitrogenase activity in cyanobacterium, Nostoc Anth. Indian Journal of Experimental Biology, 29 (6) : 496-497.
  • [10]. Prosperi C, Luna C and Valiente EF. 1993. Influence of pH light intensity and oxygen on the short-term effect of ammonium on nitrogenase activity of cyanobacteria from rice fields. Environmental and Experimental Botany, 33 (4): 545-552.
  • [11]. Singh HN, Rai UN, Rao VV and Bagchi SN. 1983 Evidence for ammonia as an inhibitor of heterocyst and nitrogenase formation in the cyanobacterium Anabaena cycadeae. Biochemical and Biophysical Research Communications, 111(1): 180-187.
  • [12]. Juan LR, Francisco M and Miguel GG. 1985. Regulation of nitrogenase levels in Anabaena sp. and other filamentous cyanobacteria Archives of Microbiology, 141:105-111.
  • [13]. Haselkorn R. 1978. Heterocysts. Annual Review of Plant Physiology and Plant Molecular Biology, 29: 319-344.
  • [14]. Stewart WDP. 1980. Some aspects of structure. and function in N2 fixing cyanobacteria. Annual Review Microbiology, 34:497-536.
  • [15]. Meeks JC, Wycoff KL, Chapman JS and Enderlin CS. 1983. Regulation of expression of nitrate and dinitrogen assimilation by Anabaena species. Applied and Environmental Microbiology, 45 (4): 1351-1359.
  • [16]. Moisander PH and Paerl HW. 2000. Growth, primary productivity and nitrogen fixation potential of Nodularia spp. in water from a subtropical estuary in the United States. Journal of Phycology, 36: 645-658.
  • [17]. Sroga GE. 1997. Regulation of nitrogen fixation by different nitrogen sources in the filamentous nonheterocystous cyanobacterium Microcoleus sp. FEMS Microbiology Letters, 153: 11-15.
  • [18]. Anneliese E, Reich S, and Böger P. 1990. Modification of dinitrogenase reductase in the cyanobacterium Anabaena variabilis due to C starvation and ammonia. Journal of Bacteriology, 172 -2:748-755.
  • [19]. Jose MN, Herrero A and Flores3E. 1991. Control of nitrogenase mRNA levels by products of nitrate assimilation in the cyanobacterium Anabaena sp. strain PCC7120. Plant Physiology, 97: 825-828.
  • [20]. Bottomley PJ, Grillo JF, Baalen CV and Tabita FR. 1979. Synthesis of nitrogenase and heterocysts by Anabaena, sp. CA in the presence of high levels of ammonia. Journal of Bacteriology, 140-3: 938-943.
  • [21]. Valiente EF, Quesada A, Prosperi C, Nieva M, Leganes F and Ucha A. 1997. Short and long term effects of ammonium on photodependent nitrogen fixation in wetland rice fields of Spain. Biology and Fertility of Soils, 24: 353-357.
  • [22]. Turpin DH, Edie SA and Canvin DT. 1984. In vivo nitrogenase regulation by ammonium and methylamine and the effect of M.8X on ammonium transport in Anabaena flos-aquae. Plant Physiology, 74: 701-704.
  • [23]. Adhya TK, Pattnaik P, Satpathy SN, Kumaraswamy S, and Sethunathan N. 1998. Influence of phosphorus application on methane emission and production in flooded paddy soils Soil Biology & Biochemistry, 30-2: 177-181.
  • [24]. Wilson JT and Alexander M. 1979. Effect of soil nutrient status and pH on nitrogen-fixing algae in flooded soils. Soil Science Society of America Journal, 43: 936-939.
  • [25]. Turid L. 1999. Environmental factors influencing the nitrogen fixation activity of free- living terrestrial cyanobacteria from a hight arctic area. Canadian Journal of Microbiology, 45-(7): 573-581.
  • [26]. Basilier K, Granhall U and Stenström TA. 1978. Nitrogen fixation in wet minetrophic moss communities of a subarctic mire. Oikos, 31: 236-246.
  • [27]. Chapin DM, Bliss LC and Bledsoe LJ. 1991. Environmental regulation of nitrogen fixation in a high arctic lowland ecosystem. Canadian Journal of Botany, 69: 2744-2755.
  • [28]. Lehtimaki J, Moisander P, Sivonen K and Kononen K. 1997. Growth, nitrogen fixation and nodularin production by two Baltic Sea cyanobacteria. Applied and Environmental Microbiology, 63 (5): 1647-1656.
  • [29]. Huber AL. 1985. Factors affecting the germination of akinetes of Nodularia spumigena. Applied and Environmental Microbiology, 49 (1):73
  • [30]. Leganes F, Carreres R, Tome RG, Nieva M, Quesada A, Sendra J and Valiente EF. 2001. Effect of phosphate fertilisation, straw incorporation, insecticide application and inoculation with cyanobacteria on rice productivity. Invest. Agr. Prod. Prot.Veg., 16 (2): 273-282.
  • [31]. Jianyi M, Ligen X, Shufeng W, Rongquan Z, Shuihu J, Songqi H and Youjun H. 2002. Toxicity of 40 Herbicides to the Green Alga Chlörella vulgaris. Exotoxicology and Environmental Safety, 51: 128-132.
  • [32]. Yan GA, Yan X and Wu W. 1997. Effects of the herbicide molinate on mixotrophic growth, photosynthetic pigments and protein content of Anabaena sphaerica under different light conditions. Ecotoxicology and Environmental Safety, 38 (2): 144-149.
  • [33]. Mansour FA, Soliman, ARI., Shaaban-Desouki, SA. and Hussein, MH. 1994. Effect of herbicides on cyanobacteria. I. Changes in carbohydrate content,Pmase and GOT activities in Nostoc sp. and Anabaena sp. Phykos, 33:153-162.
  • [34]. Caux PY., Menard L. and Kent RA. 1996. Comparative study of the effects of MCPA, butylate, atrazine and cyanozine on" Selenastrum of sp. Environmental Pollution, 92: 219-225.
  • [35]. Narwal SS. 2000, Weed Management in Rice. Critical Review in Plant Sciences, 19(3): 249-266.
  • [36]. Gianessi LP, Silvers CS, Sankula S and Carpenter JE.2002.,Herbicide Tolerant Rice. Plant Biotechnology:Current and Potential Impact for Improving PestManagement in U.S. Agriculture an analysis of 40Case Studies.( www. ncfap. Org), 1-14.
  • [37]. Pratley JE, Broster JC, Flower GE and Flower RF.2001. Herbicide resistance in the rice growing regions of Southern Australia. A report for the Rural Industries Research and Development Corporation, No: 01/ 40:1-11.
  • [38]. Singh LJ and Tiwari DN. 1988. Effects of selected rice field herbicides on photosynhesis, respiration and nitrogen assimilating enzyme systems of paddy soil diazotrophic cynanobacteria. Pesticide Biochemistry and Physiology, 31: 120-128.
  • [39]. Castenholz RW. 1988. Culturing methods for cyanobacteria. Methods in Enzymology, 167: 68-113.
  • [40]. Fogg GE, Stewart WDP, Fay P and Walsby AE. 1973.Culture, nutrition and growth. The Blue Green Algae, Academic Press, London, NewYork. 129-142.
  • [41]. Rippka R. 1988a. Isolation and purification of cyanobacteria. Methods in Enzymology, 167: 3-27.
  • [42]. Burlage RS, Atlas R, Stahl D, Geesey G, and Sayler G., 1998. Techniques in Microbial Ecology Oxford University Press, America, 8-14.
  • [43]. Cappuccino JG and Sherman N. 2001. Microbiology A Laboratory Manual, Sixth Edition, Benjamin Cummings, 119, S. Francisco.
  • [44]. Huber AL 1986. Nitrogen fixation by Nodularia spumigena Mertens. 2: Laboratory studies.Hydrobiologia, 133: 193-202.
  • [45]. Anonymus. 2002. Weed management in the cultured plants growing regions of Corum. Head- Office of Agriculture, page 1, Ankara, Türkiye.
  • [46], Jin CY, Song LR and Li SH. 1996. The mixotrophic growth of Anabaena sp. Acta Hydrobiol. Sin. 2: 134-137.
  • [47].Pandey AK. 1985. Effects of propanil on growth and cell constituents of Nostoc calciola. Pesticide Biochemistry and Physiology, 23:157-162.
  • [48].Abou- Waly H, Abou- Setta MM, Nigg HN and Mallory LL. 1991. Growth response of freshwater algae, Anabaena flos- aquae and Selenastrum capricornutum, to atrazine and hexazinone herbicides.Bulletin of Environmental Contamination and Toxicology, 46: 223 - 229.
APA KURUCUOĞLU O, Dönmez G, DÖNMEZ S (2007). Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. , 57 - 62.
Chicago KURUCUOĞLU Okmen Gülten,Dönmez Gönül,DÖNMEZ Sedat Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. (2007): 57 - 62.
MLA KURUCUOĞLU Okmen Gülten,Dönmez Gönül,DÖNMEZ Sedat Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. , 2007, ss.57 - 62.
AMA KURUCUOĞLU O,Dönmez G,DÖNMEZ S Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. . 2007; 57 - 62.
Vancouver KURUCUOĞLU O,Dönmez G,DÖNMEZ S Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. . 2007; 57 - 62.
IEEE KURUCUOĞLU O,Dönmez G,DÖNMEZ S "Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields." , ss.57 - 62, 2007.
ISNAD KURUCUOĞLU, Okmen Gülten vd. "Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields". (2007), 57-62.
APA KURUCUOĞLU O, Dönmez G, DÖNMEZ S (2007). Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. Journal of Applied Biological Sciences, 1(1), 57 - 62.
Chicago KURUCUOĞLU Okmen Gülten,Dönmez Gönül,DÖNMEZ Sedat Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. Journal of Applied Biological Sciences 1, no.1 (2007): 57 - 62.
MLA KURUCUOĞLU Okmen Gülten,Dönmez Gönül,DÖNMEZ Sedat Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. Journal of Applied Biological Sciences, vol.1, no.1, 2007, ss.57 - 62.
AMA KURUCUOĞLU O,Dönmez G,DÖNMEZ S Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. Journal of Applied Biological Sciences. 2007; 1(1): 57 - 62.
Vancouver KURUCUOĞLU O,Dönmez G,DÖNMEZ S Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields. Journal of Applied Biological Sciences. 2007; 1(1): 57 - 62.
IEEE KURUCUOĞLU O,Dönmez G,DÖNMEZ S "Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields." Journal of Applied Biological Sciences, 1, ss.57 - 62, 2007.
ISNAD KURUCUOĞLU, Okmen Gülten vd. "Influence of nitrate, phosphate and herbicide stresses on nitrogenase activity and growth of cyaobacteria isolated from paddy fields". Journal of Applied Biological Sciences 1/1 (2007), 57-62.
Sayfa Oluşturulma Tarihi
22-04-2024 13:0

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