The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey

SIRIKEN, BELGİN

The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey

Belgin SIRIKEN (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Afyon, Türkiye)

Turkish Journal of Veterinary and Animal Sciences

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Yıl: 2004 Cilt: 28 Sayı: 3 Sayfa Aralığı: 489 - 495 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey

Öz:

Bu çalışma, Aydın ili'nde tüketime sunulan kıymalarda Y. enterocolitica ve diğer Yersinia türlerinin varlığını araştırmak ve izole edilen Y. enterocolitica suşlarının patojenitesini saptamak amacıyla planlandı. Bu amaçla, Haziran 2000 - Ocak 2001 tarihleri arasında 15 farklı kasap ve 3 süpermarketten 61 kıyma örneği alındı, izolasyon amacıyla, soğukta önzenginleştirme ve selektif zenginleştirme işlemlerini kapsayan iki basamaklı zenginleştirme işlemi uygulandı, izole edilen Y. enterocolitica suşlarının patojenitesini saptamak amacıyla eskülin hidrolizi, şalisin fermentasyonu, kalsiyum bağlama, kristal violet ve Kongo red bağlama yöntemlerini kapsayan 5 farklı test yapıldı. Sonuçta, analiz edilen 61 kıyma örneklerinin 20'sinden (% 32,8) 3 farklı Yersinia türü izole ve identifiye edildi. Pozitif örnekler arasında 17 (% 27,9) Y. enterocolitica, Z (% 3,3) Y. intermedia, l (% l ,6) Y. frederiksenii olarak identifiye edildi. Yapılan virulens marker testleri sonucu, izole edilen Y. enterocolitica izolatlarının hiçbirisinin şüpheli virulent suşlar olmadıkları bulundu.

Anahtar Kelime: virülans halk sağlığı Aydın patojenite Yersinia enterocolitica mikrobiyal bulaşıklık gıda bulaşıklılığı gıda güvenirliği sığır eti Türkiye kıyma

Konular: Ziraat Mühendisliği

Sığır kıymalarında Yersinia enterocolitica ve diğer Yersinia türlerinin varlığı ve patogenetesinin belirlenmesi

Öz:

The objective of this study was to investigate the prevalence of Y. enterocolitica and other Yersinia species in raw ground beef samples and to determine their potential virulence. For this purpose, 61 ground beef samples purchased from 15 different butcher's shops and 3 supermarkets in Aydın province in Turkey were examined between June 2000 and January 2001. The presence of Y. enterocolitica and other Yersinia species was determined using 2 stage enrichment procedures including pre-enrichment (cold) and selective enrichment procedures. All positive cultures of Y. enterocolitica were also tested for markers of virulence including hydrolysis of esculin, fermentation of salicin, calcium binding, crystal violet and Congo red binding assays. Yersinia spp. were detected in 20 of 61 (32.8%) samples. Among the positive samples, 17 (27.9%) were identified as Y. enterocolitica, 2 (3.3%) as Y. intermedia and 1 (1.6%) as Y. frederiksenii. As a result of virulence marker tests, none of the Y. enterocolitica isolates were presumptively virulent.

Anahtar Kelime: beef Turkey ground beef virulence public health Aydin pathogenicity Yersinia enterocolitica microbial contamination food contamination food safety

Konular: Ziraat Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SIRIKEN B (2004). The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. , 489 - 495.
Chicago	SIRIKEN BELGİN The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. (2004): 489 - 495.
MLA	SIRIKEN BELGİN The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. , 2004, ss.489 - 495.
AMA	SIRIKEN B The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. . 2004; 489 - 495.
Vancouver	SIRIKEN B The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. . 2004; 489 - 495.
IEEE	SIRIKEN B "The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey." , ss.489 - 495, 2004.
ISNAD	SIRIKEN, BELGİN. "The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey". (2004), 489-495.

APA	SIRIKEN B (2004). The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. Turkish Journal of Veterinary and Animal Sciences, 28(3), 489 - 495.
Chicago	SIRIKEN BELGİN The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. Turkish Journal of Veterinary and Animal Sciences 28, no.3 (2004): 489 - 495.
MLA	SIRIKEN BELGİN The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. Turkish Journal of Veterinary and Animal Sciences, vol.28, no.3, 2004, ss.489 - 495.
AMA	SIRIKEN B The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(3): 489 - 495.
Vancouver	SIRIKEN B The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey. Turkish Journal of Veterinary and Animal Sciences. 2004; 28(3): 489 - 495.
IEEE	SIRIKEN B "The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey." Turkish Journal of Veterinary and Animal Sciences, 28, ss.489 - 495, 2004.
ISNAD	SIRIKEN, BELGİN. "The presence of Yersinia enterocolitica and other Yersinia species in ground beef in Aydın, Turkey". Turkish Journal of Veterinary and Animal Sciences 28/3 (2004), 489-495.