The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region

CAKİNA, Suat; SİPAHİ, TAMMAM; GULDIKEN, Baburhan; TURGUT, Nilda; GÜLDİKEN, Sibel; Özkan, Hülya; ŞENER, Seralp; Ustundag, Sedat; BUDAK, METİN

The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region

Tammam SİPAHİ, (Trakya Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Edirne, Türkiye)

BABÜRHAN FEYZULLAH GÜLDİKEN, (Trakya Üniversitesi, Tıp Fakültesi, Nöroloji Anabilim Dalı, Edirne, Türkiye)

Sibel GÜLDİKEN, (Trakya Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Gastroenteroloji BD. Edirne, Türkiye)

Sedat ÜSTÜNDAĞ, (Trakya Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, Gastroenteroloji BD. Edirne, Türkiye)

Nilda TURGUT, (Trakya Üniversitesi, Tıp Fakültesi, Nöroloji Anabilim Dalı, Edirne, Türkiye)

Metin BUDAK, (Trakya Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Edirne, Türkiye)

Suat CAKİNA, (Trakya Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Edirne, Türkiye)

Hülya ÖZKAN, (Sosyal Sigorta Hastanesi, Nöroloji Kliniği, Edirne, Türkiye)

Seralp ŞENER (Trakya Üniversitesi, Tıp Fakültesi, Biyofizik Anabilim Dalı, Edirne, Türkiye)

Trakya Üniversitesi Tıp Fakültesi Dergisi

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Yıl: 2009 Cilt: 26 Sayı: 1 Sayfa Aralığı: 1 - 8 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region

Öz:

Amaç: Bu çalışmanın amacı, Trakya bölgesinde yaşayan iskemik inme geçirmiş hastalarda ACE insersiyon/delesyon (I/D) ve AT1R (A1166C) gen polimorfizmlerinin sıklığını, vasküler risk faktörleri ve inme alt-grupları ile ilişkisini araştırmaktır.Hastalar ve Yöntemler: Çalışmaya 162 iskemik inme geçirmiş hasta ile 146 sağlıklı olgu alındı. İskemik inme hastaları, ORG 10172 Akut İnme Tedavisi (TOAST) kriterlerine göre büyük ve küçük damar hastalığı olarak inme alt gruplarına ayrıldı. ACE I/D polimorfizmi polimeraz zincir reaksiyonu (PZR), AT1R (A1166C) gen polimorfizmi ise PZR ve restriksiyon fragment uzunluk polimorfizmi (RFLP) yöntemleri kullanılarak yapıldı.Bulgular: Hasta grubundaki ACE I/D genotip dağılımı (DD=34.0%, ID=50.0%, II=16.0%), kontrol grubu ile karşılaştırıldığında (DD=34.3%, ID=49.7%, II=16.1%) fark bulunmadı. Ayrıca hasta grubundaki AT1R (A1166C) genotip dağılımları ile (AA=58.0%, CA=34.6% ve CC=7.4%) kontrol grubu ile karşılaştırıldığında (AA=60.1%, CA=35.7% ve CC=4.2%) anlamlı fark saptanmadı. Her iki inme alt grubu arasında ACE I/D ve AT1R (A1166C) polimorfizmlerinin dağılımı açısından farklılık bulunmadı.Sonuç: Çalışmamızda Trakya bölgesinde yaşayan insanlarda ACE I/D ve AT1R (A1166C) gen polimorfizmlerinin iskemik inme gelişmesinde genetik risk faktörleri olmadıkları belirlendi.

Anahtar Kelime: Reseptör, anjiotensin, tip 1 Risk faktörleri Anjiyotensin dönüştürücü enzim inhibitörleri Polimorfizm, genetik Renin-anjiyotensin sistemi İnme

Konular: Genel ve Dahili Tıp

Anjiyotensin dönüştürücü enzim ve anjiyotensin II tip 1 reseptörü gen polimorfizmlerinin Trakya bölgesindeki Türk hastalarda görülen iskemik inme ile ilişkisi

Öz:

Objectives: The aim of this study was to investigate the frequency of ACE insertion/deletion (I/D) and AT1R (A1166C) gene polymorphisms in ischemic stroke patients in Trakya region and the relation between these gene polymorphisms and stroke subtypes and vascular risk factors.Patients and Methods: The study involved 162 patients with ischemic stroke and 146 control subjects. Ischemic stroke patients were divided into large and small vessel disease subgroups according to ORG 10172 in Acute Stroke Treatment TOAST criteria. The ACE I/D polymorphism was investigated using polymerase chain reaction (PCR), and the AT1R (A1166C) polymorphism was identified using PCR and restriction fragment length polymorphism (RFLP) assay.Results: The ACE I/D genotype distribution in patients (DD=34.0%, ID=50.0%, II=16.0%) did not differ from those in controls (DD=34.3%, ID=49.7%, II=16.1%). The AT1R A1166C genotype distribution in patients (AA=58.0%, CA=34.6%, CC=7.4%) did not significantly differ from those in controls (AA=60.1%, CA=35.7%, CC=4.2%). There was also no difference among the stroke subgroups regarding the distribution of ACE I/D and AT1R (A1166C) polymorphisms.Conclusion: Our results show that ACE I/D and AT1R (A1166C) gene polymorphisms were not genetic risk factors for ischemic stroke in subjects in Trakya region.

Anahtar Kelime: Polymorphism, Genetic Renin-Angiotensin System Stroke Receptor, Angiotensin, Type 1 Risk Factors Angiotensin-Converting Enzyme Inhibitors

Konular: Genel ve Dahili Tıp

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SİPAHİ T, GULDIKEN B, GÜLDİKEN S, Ustundag S, TURGUT N, BUDAK M, CAKİNA S, Özkan H, ŞENER S (2009). The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. , 1 - 8.
Chicago	SİPAHİ TAMMAM,GULDIKEN Baburhan,GÜLDİKEN Sibel,Ustundag Sedat,TURGUT Nilda,BUDAK METİN,CAKİNA Suat,Özkan Hülya,ŞENER Seralp The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. (2009): 1 - 8.
MLA	SİPAHİ TAMMAM,GULDIKEN Baburhan,GÜLDİKEN Sibel,Ustundag Sedat,TURGUT Nilda,BUDAK METİN,CAKİNA Suat,Özkan Hülya,ŞENER Seralp The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. , 2009, ss.1 - 8.
AMA	SİPAHİ T,GULDIKEN B,GÜLDİKEN S,Ustundag S,TURGUT N,BUDAK M,CAKİNA S,Özkan H,ŞENER S The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. . 2009; 1 - 8.
Vancouver	SİPAHİ T,GULDIKEN B,GÜLDİKEN S,Ustundag S,TURGUT N,BUDAK M,CAKİNA S,Özkan H,ŞENER S The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. . 2009; 1 - 8.
IEEE	SİPAHİ T,GULDIKEN B,GÜLDİKEN S,Ustundag S,TURGUT N,BUDAK M,CAKİNA S,Özkan H,ŞENER S "The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region." , ss.1 - 8, 2009.
ISNAD	SİPAHİ, TAMMAM vd. "The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region". (2009), 1-8.

APA	SİPAHİ T, GULDIKEN B, GÜLDİKEN S, Ustundag S, TURGUT N, BUDAK M, CAKİNA S, Özkan H, ŞENER S (2009). The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. Trakya Üniversitesi Tıp Fakültesi Dergisi, 26(1), 1 - 8.
Chicago	SİPAHİ TAMMAM,GULDIKEN Baburhan,GÜLDİKEN Sibel,Ustundag Sedat,TURGUT Nilda,BUDAK METİN,CAKİNA Suat,Özkan Hülya,ŞENER Seralp The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. Trakya Üniversitesi Tıp Fakültesi Dergisi 26, no.1 (2009): 1 - 8.
MLA	SİPAHİ TAMMAM,GULDIKEN Baburhan,GÜLDİKEN Sibel,Ustundag Sedat,TURGUT Nilda,BUDAK METİN,CAKİNA Suat,Özkan Hülya,ŞENER Seralp The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. Trakya Üniversitesi Tıp Fakültesi Dergisi, vol.26, no.1, 2009, ss.1 - 8.
AMA	SİPAHİ T,GULDIKEN B,GÜLDİKEN S,Ustundag S,TURGUT N,BUDAK M,CAKİNA S,Özkan H,ŞENER S The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. Trakya Üniversitesi Tıp Fakültesi Dergisi. 2009; 26(1): 1 - 8.
Vancouver	SİPAHİ T,GULDIKEN B,GÜLDİKEN S,Ustundag S,TURGUT N,BUDAK M,CAKİNA S,Özkan H,ŞENER S The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region. Trakya Üniversitesi Tıp Fakültesi Dergisi. 2009; 26(1): 1 - 8.
IEEE	SİPAHİ T,GULDIKEN B,GÜLDİKEN S,Ustundag S,TURGUT N,BUDAK M,CAKİNA S,Özkan H,ŞENER S "The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region." Trakya Üniversitesi Tıp Fakültesi Dergisi, 26, ss.1 - 8, 2009.
ISNAD	SİPAHİ, TAMMAM vd. "The association of gene polymorphisms of the angiotensin- converting enzyme and angiotensin II receptor type 1 with ischemic stroke in Turkish subjects of Trakya region". Trakya Üniversitesi Tıp Fakültesi Dergisi 26/1 (2009), 1-8.