Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats

Ersoy, Pamir Eren; AYKOL, Şükrü; ÖZTANIR, Namık; EGEHAN, İbrahim; ERKAL, Haldun Ş.; ÖZTÜRK, Ayşen S.; MEMİŞ, Leyla

Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats

Ayşen S. ÖZTÜRK, (Gazi Üniversitesi, Tıp Fakültesi, Radyasyon Onkoloji Anabilim Dalı, Ankara, Türkiye)

Haldun Ş. ERKAL, (Gazi Üniversitesi, Tıp Fakültesi, Radyasyon Onkoloji Anabilim Dalı, Ankara, Türkiye)

Leyla MEMİŞ, (Gazi Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Ankara, Türkiye)

Namık ÖZTANIR, (Gazi Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

Pamir Eren Ersoy, (Ankara Atatürk Eğitim ve Araştırma Hastanesi, Genel Cerrahi Kliniği, Ankara, Türkiye)

Şükrü AYKOL, (Gazi Üniversitesi, Tıp Fakültesi, Nöroşirürji Anabilim Dalı, Ankara, Türkiye)

İbrahim EGEHAN (Gazi Üniversitesi, Tıp Fakültesi, Radyasyon Onkoloji Anabilim Dalı, Ankara, Türkiye)

Uluslararası Hematoloji-Onkoloji Dergisi

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Yıl: 2009 Cilt: 19 Sayı: 2 Sayfa Aralığı: 88 - 94 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats

Öz:

Bu çalışmada melatoninin radyoterapi ile uyarılan orofarengeal mukozite karşı bir koruyucu olarak etkilerinin ratlarda bir pinealektomi modelinde histopatolojik değerlendirmesi sunulmaktadır. Otuz iki Wistar türü sıçan dört gruba bölünmüştür. Grup 1’deki sıçanlara pinealektomi uygulanmış, melatonin verilmiş ve radyoterapi uygulanmıştır. Grup 2’deki sıçanlara pinealektomi uygulanmış, melatonin verilmemiş ve radyoterapi uygulanmıştır. Grup 3’teki sıçanlara sahte pinealektomi uygulanmış, melatonin verilmiş ve radyoterapi uygulanmıştır. Grup 4’teki sıçanlara sahte pinealektomi uygulanmış, melatonin verilmemiş ve radyoterapi uygulanmıştır. Radyoterapi uygulamasından 6 gün sonar sıçanlara ötenazi uygulanmıştır. Orofarengeal dokular kesilerek ayrılmış ve melatoninin radyoterapi ile uyarılan orofarengeal mukozite karşı bir koruyucu olarak etkileri açısından histopatolojik değerlendirme yapılmıştır. Ortalama vasküler kalibrasyon skoru Grup 1 için 2.3, Grup 2 için 2.3, Grup 3 için 1.8 ve Grup 4 için 2.1 idi (p= 0.44). Ortalama vasküler permeabilite skoru Grup 1 için 2.4, Grup 2 için 2.4, Grup 3 için 2.0 ve Grup 4 için 2.1 idi (p= 0.61). Ortalama lökosit emigrasyon skoru Grup 1 için 2.0, Grup 2 için 2.0, Grup 3 için 1.3 ve Grup 4 için 1.6 idi (p= 0.02). Orofarengeal mukozit genel skoru Grup 1 için 6.6, Grup 2 için 6.6, Grup 3 için 5.1 ve Grup 4 için 5.8 idi (p= 0.15). Bu çalışma melatoninin radyoterapi ile uyarılan orofarengeal mukozite karşı bir koruyucu olarak potansiyel etkilerini ortaya koymaktadır.

Anahtar Kelime: Pineal bez Radyasyon hasarı, deneysel Melatonin Mukus membran Radyasyon hasarı Modeller, hayvan Sıçan, Wistar Histopatoloji

Konular: Onkoloji

Sıçanlarda bir pinealektomi modelinde radyoterapiye bağlı orofaringeal mukozit üzerine melatoninin koruyucu etkisinin histopatolojik olarak değerlendirilmesi

Öz:

This study presents the histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis induced by radiation therapy in a pinealectomy model in rats. Thirty-two Wistar rats were divided into four groups. The rats in Group 1 underwent pinealectomy, received melatonin and underwent radiation therapy. The rats in Group 2 underwent pinealectomy, received no melatonin and underwent radiation therapy. The rats in Group 3 underwent sham pinealectomy, received melatonin and underwent radiation therapy. The rats in Group 4 underwent sham pinealectomy, received no melatonin and underwent radiation therapy. The rats underwent euthanasia at six days following radiation therapy. The oropharyngeal tissues were dissected and histopathological evaluation was performed regarding the effects of melatonin as a protectant against oropharyngeal mucositis induced by radiation therapy. Mean item scores for vascular calibration were 2.3 points for Group 1, 2.3 points for Group 2, 1.8 points for Group 3 and 2.1 points for Group 4 (p= 0.44). Mean item scores for vascular permeability were 2.4 points for Group 1, 2.4 points for Group 2, 2.0 points for Group 3 and 2.1 points for Group 4 (p= 0.61). Mean item scores for leukocyte emigration were 2.0 points for Group 1, 2.0 points for Group 2, 1.3 points for Group 3 and 1.6 points for Group 4 (p= 0.02). Mean overall scores for oropharyngeal mucositis were 6.6 points for Group 1, 6.6 points for Group 2, 5.1 points for Group 3 and 5.8 points for Group 4 (p= 0.15). This study elicits the potential effects of melatonin as a protectant against oropharyngeal mucositis induced by radiation therapy.

Anahtar Kelime: Mucous Membrane Radiation Injuries Models, Animal Rats, Wistar Histopathology Pineal Gland Radiation Injuries, Experimental Melatonin

Konular: Onkoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖZTÜRK A, ERKAL H, MEMİŞ L, ÖZTANIR N, Ersoy P, AYKOL Ş, EGEHAN İ (2009). Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. , 88 - 94.
Chicago	ÖZTÜRK Ayşen S.,ERKAL Haldun Ş.,MEMİŞ Leyla,ÖZTANIR Namık,Ersoy Pamir Eren,AYKOL Şükrü,EGEHAN İbrahim Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. (2009): 88 - 94.
MLA	ÖZTÜRK Ayşen S.,ERKAL Haldun Ş.,MEMİŞ Leyla,ÖZTANIR Namık,Ersoy Pamir Eren,AYKOL Şükrü,EGEHAN İbrahim Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. , 2009, ss.88 - 94.
AMA	ÖZTÜRK A,ERKAL H,MEMİŞ L,ÖZTANIR N,Ersoy P,AYKOL Ş,EGEHAN İ Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. . 2009; 88 - 94.
Vancouver	ÖZTÜRK A,ERKAL H,MEMİŞ L,ÖZTANIR N,Ersoy P,AYKOL Ş,EGEHAN İ Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. . 2009; 88 - 94.
IEEE	ÖZTÜRK A,ERKAL H,MEMİŞ L,ÖZTANIR N,Ersoy P,AYKOL Ş,EGEHAN İ "Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats." , ss.88 - 94, 2009.
ISNAD	ÖZTÜRK, Ayşen S. vd. "Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats". (2009), 88-94.

APA	ÖZTÜRK A, ERKAL H, MEMİŞ L, ÖZTANIR N, Ersoy P, AYKOL Ş, EGEHAN İ (2009). Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. Uluslararası Hematoloji-Onkoloji Dergisi, 19(2), 88 - 94.
Chicago	ÖZTÜRK Ayşen S.,ERKAL Haldun Ş.,MEMİŞ Leyla,ÖZTANIR Namık,Ersoy Pamir Eren,AYKOL Şükrü,EGEHAN İbrahim Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. Uluslararası Hematoloji-Onkoloji Dergisi 19, no.2 (2009): 88 - 94.
MLA	ÖZTÜRK Ayşen S.,ERKAL Haldun Ş.,MEMİŞ Leyla,ÖZTANIR Namık,Ersoy Pamir Eren,AYKOL Şükrü,EGEHAN İbrahim Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. Uluslararası Hematoloji-Onkoloji Dergisi, vol.19, no.2, 2009, ss.88 - 94.
AMA	ÖZTÜRK A,ERKAL H,MEMİŞ L,ÖZTANIR N,Ersoy P,AYKOL Ş,EGEHAN İ Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. Uluslararası Hematoloji-Onkoloji Dergisi. 2009; 19(2): 88 - 94.
Vancouver	ÖZTÜRK A,ERKAL H,MEMİŞ L,ÖZTANIR N,Ersoy P,AYKOL Ş,EGEHAN İ Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats. Uluslararası Hematoloji-Onkoloji Dergisi. 2009; 19(2): 88 - 94.
IEEE	ÖZTÜRK A,ERKAL H,MEMİŞ L,ÖZTANIR N,Ersoy P,AYKOL Ş,EGEHAN İ "Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats." Uluslararası Hematoloji-Onkoloji Dergisi, 19, ss.88 - 94, 2009.
ISNAD	ÖZTÜRK, Ayşen S. vd. "Histopathological evaluation of the effects of melatonin as a protectant against oropharyngeal mucositis ınduced by radiation therapy in a pinealectomy model in rats". Uluslararası Hematoloji-Onkoloji Dergisi 19/2 (2009), 88-94.