A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities

SEVIM, ALI; DEMİRBAĞ, ZİHNİ; Demir, Ismail

A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities

Ali SEVİM, (Rize Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Zihni DEMİRBAĞ, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

İsmail DEMİR (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

Turkish Journal of Agriculture and Forestry

4 0

Yıl: 2010 Cilt: 34 Sayı: 4 Sayfa Aralığı: 333 - 342 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities

Öz:

Agrotis segetumSchiff. (Lepidoptera: Noctuidae) Türkiye’de hemen hemen bütün sebzelerin en önemli zararlılarından birisidir. Bu çalışmada, daha etkili ve güvenli biyolojik mücadele etmeni bulmak için Agrotis segetum’un bakteriyal florası Karadeniz Bölgesi’ndeki sebze tarlarında farklı populasyonlardan toplanan larvalar üzerinde araştırıldı. Bu populasyonlardan dokuz bakteri izolatı kültüre alındı. Bu bakterilerden yedi tanesi tür seviyesinde, diğerleri ise cins seviyesinde tanımlandı ve karakterize edildi. Bakteriyal isolatların morfolojik, fizyolojik ve biyokimyasal özellikleri, rutin ve geleneksel teknikler kullanılarak belirlendi. İzolatların karakterizasyonu, VITEK 32 bakteriyal tanımlama sistemi kullanılarak da gerçekleştirildi. Buna ilaveten, bütün izolatların NCBI genetik veritabanında karşılaştırmalarını yapmak ve ilişkili türler ile filogenetik yakınlıklarını belirlemek için 16S rRNA gen sekans analizleri yapıldı. Sonuç olarak, izolatlar Bacillus cereus (Ags1), Bacillus sp. (Ags2), B. megaterium (Ags3), Enterobacter aerogenes (Ags4), Acinetobacter calcoaceticus (Ags5), Enterobacter sp. (Ags6), Pseudomonas putida (Ags7), Enterococcus gallinarum (Ags8) ve Stenotrophomonas maltophilia (Ags9) olarak tanımlandı. Bütün izolatlar A. segetum’un florasında ilk kez belirlendi. Ayrıca, bakteriyal izolatların insektisidal etkileri A. segetum larvalarına karşı test edildi. Uygulamadan 8 gün sonra Enterococcus gallinarum (Ags8)’un en yüksek aktiviteye (%60) sebep olduğu bulundu.

Anahtar Kelime: taksonomi entomopatojen bakteriler Lepidoptera Agrotis segetum bakteriyel böcel öldürücüler biyolojik kontrol ajanları sebzeler Türkiye bitki zararlıları böcek öldürücü etkisi ribozomal RNA filogenetik entomopatojen

Konular: Orman Mühendisliği

Agrotis segetum Schiff. (Lepidoptera: Noctuidae)’un bakterileri ve bu bakterilerin insektisidal etkileri üzerine yeni bir çalışma

Öz:

Agrotis segetum Schiff. (Lepidoptera: Noctuidae) is one of the most serious pests of nearly all vegetables in Turkey. In this study, to find a more effective and safe biological control agent, the bacterial flora of Agrotis segetumwere investigated in larvae collected from different populations in the vegetable fields of the Black Sea region of Turkey, and 9 bacterial isolates were cultured from these populations. Seven of these bacteria were determined and characterized at the species level and the rest were characterized at the genus level. Morphological, physiological, and biochemical characteristics of the bacterial isolates were determined by conventional and routine techniques. Characterization of the isolates was also performed using the VITEK 32 bacterial identification system. In addition, a 16S rRNA gene sequence was determined for these isolates to query against the NCBI genetic database and to construct a phylogenetic tree using closely related species. Consequently, the isolates were identified as Bacillus cereus (Ags1), Bacillus sp. (Ags2), Bacillus megaterium(Ags3), Enterobacter aerogenes (Ags4), Acinetobacter calcoaceticus (Ags5), Enterobacter sp. (Ags6), Pseudomonas putida (Ags7), Enterococcus gallinarum (Ags8), and Stenotrophomonas maltophilia (Ags9). All isolates were determined the first time from the flora of A. segetum. Furthermore, the insecticidal activities of the bacterial isolates were tested against A. segetum larvae, and Enterococcus gallinarum (Ags8) was found to cause the highest insecticidal activity (60%) 8 days after application.

Anahtar Kelime: vegetables Turkey plant pests insecticidal action ribosomal RNA phylogenetics entomopathogens taxonomy entomopathogenic bacteria Lepidoptera Agrotis segetum bacterial insecticides biological control agents

Konular: Orman Mühendisliği

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SEVIM A, DEMİRBAĞ Z, Demir I (2010). A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. , 333 - 342.
Chicago	SEVIM ALI,DEMİRBAĞ ZİHNİ,Demir Ismail A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. (2010): 333 - 342.
MLA	SEVIM ALI,DEMİRBAĞ ZİHNİ,Demir Ismail A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. , 2010, ss.333 - 342.
AMA	SEVIM A,DEMİRBAĞ Z,Demir I A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. . 2010; 333 - 342.
Vancouver	SEVIM A,DEMİRBAĞ Z,Demir I A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. . 2010; 333 - 342.
IEEE	SEVIM A,DEMİRBAĞ Z,Demir I "A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities." , ss.333 - 342, 2010.
ISNAD	SEVIM, ALI vd. "A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities". (2010), 333-342.

APA	SEVIM A, DEMİRBAĞ Z, Demir I (2010). A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. Turkish Journal of Agriculture and Forestry, 34(4), 333 - 342.
Chicago	SEVIM ALI,DEMİRBAĞ ZİHNİ,Demir Ismail A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. Turkish Journal of Agriculture and Forestry 34, no.4 (2010): 333 - 342.
MLA	SEVIM ALI,DEMİRBAĞ ZİHNİ,Demir Ismail A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. Turkish Journal of Agriculture and Forestry, vol.34, no.4, 2010, ss.333 - 342.
AMA	SEVIM A,DEMİRBAĞ Z,Demir I A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. Turkish Journal of Agriculture and Forestry. 2010; 34(4): 333 - 342.
Vancouver	SEVIM A,DEMİRBAĞ Z,Demir I A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities. Turkish Journal of Agriculture and Forestry. 2010; 34(4): 333 - 342.
IEEE	SEVIM A,DEMİRBAĞ Z,Demir I "A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities." Turkish Journal of Agriculture and Forestry, 34, ss.333 - 342, 2010.
ISNAD	SEVIM, ALI vd. "A new study on the bacteria of Agrotis segetum Schiff. (Lepidoptera: Noctuidae) and their insecticidal activities". Turkish Journal of Agriculture and Forestry 34/4 (2010), 333-342.