Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish

KARİPTAŞ, ERGİN; Erdem, Belgin; Kaya, Tayfun

Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish

Belgin ERDEM, (Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırşehir, Türkiye)

Ergin KARİPTAŞ, (Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırşehir, Türkiye)

Tayfun KAYA (Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırşehir, Türkiye)

Turkish Journal of Biology

5 0

Yıl: 2010 Cilt: 34 Sayı: 4 Sayfa Aralığı: 453 - 462 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish

Öz:

Toplam 120 tatlı su balığı örneklerinin, solungaç, bağırsak, karaciğer ve deri numunelerinden izole edilen Aeromonas cinsine ait A. hydrophila, A. caviae ve A. veronii bv. sobria varlığı değerlendirilmiştir. İzole edilen 78 Aeromonas suşunun siderofor, hemolitik, pirazinamidaz, proteaz aktiviteleri ve antibiyotik direnç durumları incelenmiştir. Siderofor üretimi A. hydrophila, A. caviae suşlarının neredeyse tamamında görülürken A. veronii bv. sobria suşlarının yalnızca ikisinde gözlenmiştir. A. hydrophila and A. veronii bv. sobria suşları hemolizin üretirken A. caviae suşları nonhemolitiktir. A. hydrophila suşlarının % 92’si, A. caviae suşlarının % 91’i pirazinamid’i 48 saatten daha az bir zamanda hidroliz etmiş oysa A. veronii bv. sobria suşları yüzde beş oranında aktivite göstermiştir. Proteaz aktivitesi, A. hydrophila, ve A. veronii bv. sobria izolatlarının tamamında (% 100) bulunurken A. caviae izolatlarının % 81,8’de saptanmıştır. Bunlara ilaveten, tüm Aeromonas suşları ampisilin ve tetrasikline karşı dirençli bulunmuştur.

Anahtar Kelime: direnç proteaz Aeromonas tatlısu balıkları antibiyotikler proteinaz pirazinamit sideroforlar enzim aktivitesi balık hastalıkları hemolisin

Konular: Biyoloji

Balıklardan izole edilen hareketli Aeromonas’larda siderofor, hemolitik, proteaz, pirazinamidaz aktiviteleri ve antibiyotik direnç

Öz:

A total of 120 fresh water fish samples were evaluated for the presence of Aeromonas spp. A. hydrophila, A. caviae, and A. veronii bv. sobria were isolated from the gills, intestines, livers, and skins of fish, and 78 isolated Aeromonas spp. strains were further examined for siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance. Siderophore production was demonstrated in almost all of the A. hydrophila and A. caviae strains except for 2 strains of A. veronii bv. sobria. The strains identified as A. hydrophila and A. veronii bv. sobria were the stronger producers of hemolysin, whereas the A. caviae strains were nonhemolytic. It was found that 92% of A. hydrophila and 91% of A. caviae strains hydrolyzed pyrazinamide in under 48 h, whereas 5% of A. veronii bv. sobria strains hydrolyzed pyrazinamide. Protease activity was found in 100% of isolates of A. hydrophila and A. veronii bv. sobria, but in 81% of A. caviae isolates. In addition, all Aeromonas species were examined for antibiotic resistance patterns and were found to be resistant to ampicillin and tetracycline.

Anahtar Kelime: antibiotics proteinases pyrazinamide siderophores enzyme activity fish diseases haemolysins resistance proteases Aeromonas freshwater fishes

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Popoff M. Genus III. Aeromonas. In: Krieg NR. ed. Bergey’s Manual of Systematic Bacteriology, Volume 1. Williams & Wilkins. Baltimore; 1994: pp. 545-548.
2. Altwegg M, Steigerwalt AG, Altwegg-Bissing R et al. Biochemical identification of Aeromonas genospecies isolated from humans. J Clin Microbiol 28: 258-265, 1990.
3. Davies A, Capell C, Jehanno D et al. Incidence of foodborne pathogens on European fish. Food Control 12: 67-71, 2001.
4. Galindo CL, Sha J, Fadl AA et al. Host immune responses to Aeromonas virulence factors. Current Immun Rev 2: 13-26, 2006.
5. Galindo CL, Chopra AK. Aeromonas and Plesiomonas species. In: Doyle MP, Beuchat LR. eds. Food Microbiology: Fundamentals and Frontiers. ASM Press. Washington, D.C.; 2007: pp. 381-400.
6. Havelaar AH, During M, Versteeg JFM. Ampicillin-dextrin agar medium for the enumeration of Aeromonas species in water by membrane filtration. J Appl Bacteriol 62: 279-287, 1987.
7. Massa S, Altieri D, D’Angela A. The occurrence of Aeromonas spp. in natural mineral water and well water. Int J Food Microbiol 63: 169-173, 2001.
8. Martins LM, Marquez RF, Yano T. Incidence of toxic Aeromonas isolated from food and human infection. FEMS Immun Med Mic 32: 237-242, 2002.
9. Falcao JP, Dias AMG, Correa EF et al. Microbiological quality of ice used to refrigerate foods. Food Microbiol 19: 269-276, 2002.
10. Papageorgiou DK, Melas DS, Abrahim A et al. Growth and survival of Aeromonas hydrophila in rice pudding (milk rice) during its storage at 4 °C and 12 °C. Food Microbiol 20: 385- 390, 2003.
11. Aoki T. Motile Aeromononads (Aeromonas hydrophila). In: Woo PTK, Bruno DW. eds. Fish Diseases and Disorders, Volume 3. Viral, Bacterial and Fungal Infections Cab International; 1999: pp. 427-453.
12. Santos Y, Toranzo AE, Barja JC et al. Virulence properties and enterotoxin production of Aeromonas strain isolated from fish. Infect Immun 56: 3285-3293, 1998.
13. Zywno SR, Arceneaux JEL, Altwegg M et al. Siderophore production and DNA hybridization groups of Aeromonas spp. J Clin Microbiol 30: 619-622, 1992.
14. Barghouthi S, Young R, Olson MOJ et al. Amonabactin, a novel tryptophan- or phenylalanine containing phenolate siderophore in Aeromonas hydrophila. J Bacteriol 171: 1811-1816, 1989.
15. Pemberton JM, Kidd SP, Schmidt R. Secreted enzymes of Aeromonas. FEMS Microbiol Lett 152: 1-10, 1997.
16. Santos JA, Gonzalez CJ, Otero A et al. Hemolytic activity and siderophore production in different Aeromonas species isolated from fish. Appl Environ Microb 65: 5612-5614, 1999.
17. Soler L, Figueras MJ, Chacon MR et al. Potential virulence and antimicrobial susceptibility of Aeromonas popoffii recovered from freshwater and seawater. FEMS Immunol Med Mic 32: 243-247, 2002.
18. Lee S, Kim S, Oh Y et al. Characterization of Aeromonas hydrophila isolated from rainbow trouts in Korea. J Microbiol 1: 1-7, 2000.
19. Turnbull PCB, Lee JV, Miliotis MD et al. Enterotoxin production in relation to taxonomic grouping and source of isolation of Aeromonas species. J Clin Microbiol 19: 175-180, 1984.
20. McMahon MAS. The expression of proteinases and haemolysins by Aeromonas hydrophila under modified atmospheres. J Appl Microbiol 89: 415-422, 2000.
21. Kandolo K, Wauters G. Pyrazinamidase activity in Yersinia enterocolitica and related organisms. J Clin Microbiol 21: 980- 982, 1985.
22. Carnahan A, Hammontree L, Bourgeois L et al. Pyrazinamidase activity as a phenotypic marker for several Aeromonas spp. isolated from clinical specimens. J Clin Microbiol 28: 391-392, 1990.
23. Burgos A, Quindos G, Martinez R et al. In vitro susceptibility of Aeromonas caviae, A. hydrophila and A. sobria to fifteen antibacterial agents. Eur J Clin Microbiol Infect Dis 9: 413-417, 1990.
24. Overman TL, Janda JM. Antimicrobial susceptibility patterns of Aeromonas jandaei, A. veronii biotype veronii, A. trota and A. shubertii. J Clin Microbiol 37: 706-708, 1999.
25. Radu S, Ahmad N, Ling FH et al. Prevalence and resistance to antibiotics from Aeromonas species from retail fish in Malaysia. Int J Food Microbiol 81: 261-266, 2003.
26. Joseph SW, Carnahan A. The isolation, identification and systematics of the motile Aeromonas species. Ann Rev Fish Dis 4: 315-343, 1994.
27. Schwyn B, Neilands JB. Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160: 47-56, 1987.
28. Güven K, Mutlu MB. Properties of Bacillus cereus collected from different food sources. Turk J Biol 33: 101-108, 2009.
29. Brender R, Janda JM. Detection, quantitation and stability of the beta haemolysin of Aeromonas spp. J Med Microbiol 24: 247-251, 1987.
30. Gudmundsdottir BK. Comparison of extracellular proteases produced by Aeromonas salmonicida strains, isolated from various fish species. J Appl Bacteriol 80: 105-113, 1996.
31. Bauer AW, Kirby WMM, Sherris JC et al. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol 45: 493-496, 1966.
32. Gray SJ, Stickler DJ, Bryant TN. The incidence of virulence factors in mesophilic Aeromonas species isolated from farm animals and their environment. Epidemiol Infect 105: 277-294, 1990.
33. Carnahan AM, Joseph SW. Systematic assessment of geographically and clinically diverse aeromonads. Syst Appl Microbiol 16: 72-84, 1993.
34. Sugita H, Nakamura T, Tanaka K et al. Identification of Aeromonas species isolated from freshwater fish with the microplate hybridization method. Appl Environ Microbiol 60: 3036-3038, 1994.
35. Huys G, Kersters I, Vancanneyt M et al. Diversity of Aeromonas spp. in Flemish drinking water production plants as determined by gas-liquid chromatographic analysis of cellular fatty acid methyl esters (FAMEs). J Appl Bacteriol 78: 445-455, 1995.
36. Castro-Escarpullia G, Figueras MJ, Aguilera-Arreola G et al. Characterisation of Aeromonas spp. isolated from frozen fish intended for human consumption in Mexico. Int J Food Microbiol 84: 41-49, 2003.
37. Yucel N, Aslim B, Beyatli Y. Prevalence and resistance to antibiotics for Aeromonas species isolated from retail fish in Turkey. J Food Quality 28: 313-324, 2005.
38. Janda JM, Guthertz LS, Kokka RP et al. Aeromonas species in septicemia: laboratory characteristics and clinical observations. Clin Infect Dis 19: 77-83, 1994.
39. Gierer W, Rabsch W, Reissbrodt R. Siderophore pattern of fishpathogenic Vibrio anguiliarum, Aeromonas spp. and Pseudomonas spp. from the German Baltic coast. J Fish Dis 15: 417-423, 2006.
40. Alavandi SV, Ananthan S. Biochemical characteristics, serogroups, and virulence factors of Aeromonas species isolated from cases of diarrhoea and domestic water samples in Chennai. Indian J Med Microbiol 21: 233-238, 2003.
41. Majeed KN, MacRae IC. Effect of pH on the growth and exotoxin production by Aeromonas at refrigeration temperature. Microbios 73: 281-288, 1993.
42. Janda JM, Reitano M, Bottone EJ. Biotyping of Aeromonas isolates as a correlate to delineating a species-associated disease spectrum. J Clin Microbiol 19: 44-47, 1984.
43. Farag HESM. Incidence of hemolysin producing motile Aeromonas in some shellfish and their public health significance in Port-Said City. J Appl Sci Res 2: 972-979, 2006.
44. Yucel N, Çitak S. The occurrence, hemolytic activity and antibiotic susceptibility of motile Aeromonas spp. isolated from meat and milk samples in Turkey. J Food Sci 23: 189-200, 2003.
45. Scoaris Dde O, Colacite J, Nakamura CV et al. Virulence and antibiotic susceptibility of Aeromonas spp. isolated from drinking water. Anton Leeuw Int J G 93: 111-122, 2008.
46. Austin B, Adams C. Fish pathogens. In: Austin B, Altwegg M, Gosling PJ, Joseph S. eds. The Genus Aeromonas. Wiley. Chichester; 1996: pp. 198-243.
47. Carnahan A, Hammontree L, Bourgeois L et al. Pyrazinamidase activity as a phenotypic marker for several Aeromonas spp. isolated from clinical specimens. J Clin Microbiol 28: 391-392, 1990.
48. Wakabongo M, Bortey E, Meier FA et al. Rapid identification of motile Aeromonas. Diagn Micr Infec Dis 15: 511-515, 1992.
49. Aziz KMS, Rahim Z, Faruque ASG et al. Aeromonas hydrophila: its isolation from acute diarrhoeal illness in rural Bangladesh. Bangladesh Med Res Counc Bull 12: 49-58, 1986.
50. Hatha M, Vivekanandhan AA, Joice GJ et al. Antibiotic resistance pattern of motile Aeromonads from farm raised fresh water fish. Int J Food Microbiol 98: 131-134, 2005.
51. Ko WC, Lee HC, Chuang YC et al. Clinical features and therapeutic implications of 104 episodes of monomicrobial Aeromonas bacteraemia. J Infect 40: 267-273, 2000.
52. Goni-Urizza M, Pineau L, Capdepuy M et al. Antimicrobial resistance of mesophilic Aeromonas spp. isolated from two European rivers. J Antimicrob Chem 46: 297-301, 2000.
53. Rhodes G, Huys G, Swings J et al. Distribution of oxytetracycline resistance plasmids between aeromonads in hospital and aquaculture environments: implication of Tn1721 in dissemination of the tetracycline resistance determinant. Appl Environ Microbiol 66: 3883-3890, 2000.
54. Casas C, Anderson EC, Ojo KK et al. Characterization of pRAS1-like plasmids from atypical North American psychrophilic Aeromonas salmonicida. FEMS Microbiol Lett 242: 59-63, 2005.
55. Vivekanandhan G, Savithamani K, Hatha AAM et al. Antibiotic resistance of Aeromonas hydrophila isolated from fish and prawns. Int J Food Microbiol 76: 165-68, 2002.
56. Ansary A, Haneef RM, Torres JL et al. Plasmids and antibiotic resistance of Aeromonas hydrophila. J Fish Biol 15: 191-196, 1992.
57. Ceylan E, Berktas E, Ağaoglu Z. The occurrence and antibiotic resistance of motile Aeromonas in livestock. Trop Anim Health Pro 9175-9179, 2008.

APA	Erdem B, KARİPTAŞ E, Kaya T (2010). Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. , 453 - 462.
Chicago	Erdem Belgin,KARİPTAŞ ERGİN,Kaya Tayfun Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. (2010): 453 - 462.
MLA	Erdem Belgin,KARİPTAŞ ERGİN,Kaya Tayfun Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. , 2010, ss.453 - 462.
AMA	Erdem B,KARİPTAŞ E,Kaya T Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. . 2010; 453 - 462.
Vancouver	Erdem B,KARİPTAŞ E,Kaya T Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. . 2010; 453 - 462.
IEEE	Erdem B,KARİPTAŞ E,Kaya T "Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish." , ss.453 - 462, 2010.
ISNAD	Erdem, Belgin vd. "Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish". (2010), 453-462.

APA	Erdem B, KARİPTAŞ E, Kaya T (2010). Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology, 34(4), 453 - 462.
Chicago	Erdem Belgin,KARİPTAŞ ERGİN,Kaya Tayfun Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology 34, no.4 (2010): 453 - 462.
MLA	Erdem Belgin,KARİPTAŞ ERGİN,Kaya Tayfun Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology, vol.34, no.4, 2010, ss.453 - 462.
AMA	Erdem B,KARİPTAŞ E,Kaya T Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology. 2010; 34(4): 453 - 462.
Vancouver	Erdem B,KARİPTAŞ E,Kaya T Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish. Turkish Journal of Biology. 2010; 34(4): 453 - 462.
IEEE	Erdem B,KARİPTAŞ E,Kaya T "Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish." Turkish Journal of Biology, 34, ss.453 - 462, 2010.
ISNAD	Erdem, Belgin vd. "Siderophore, hemolytic, protease, and pyrazinamidase activities and antibiotic resistance in motile Aeromonas isolated from fish". Turkish Journal of Biology 34/4 (2010), 453-462.