Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis

KOZACI, Leyla Didem; CHIKANZA, Ian C.; Akar, Servet; ÖNEN, Fatoş; Akkoç, Nurullah; Sari, Ismail; Birlik, Merih

Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis

Leyla Didem KOZACI, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Biyokimya Anabilim Dalı, Aydın, Türkiye)

İsmail SARI, (Dokuz Eylül Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İzmir, Türkiye)

Servet AKAR, (Dokuz Eylül Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İzmir, Türkiye)

AHMET MERİH BİRLİK, (Dokuz Eylül Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İzmir, Türkiye)

Nurullah AKKOÇ, (Dokuz Eylül Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İzmir, Türkiye)

Ian C. CHIKANZA, (Bone and Joint Research Unit, Barts and the London, Queen Mary School of Medicine and Dentistry, University of London, UK)

Fatoş ÖNEN (Dokuz Eylül Üniversitesi, Tıp Fakültesi, İç Hastalıkları Anabilim Dalı, İzmir, Türkiye)

Türkiye Parazitoloji Dergisi

1 1

Yıl: 2010 Cilt: 34 Sayı: 3 Sayfa Aralığı: 156 - 160 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis

Öz:

Bu çalışmada Trichinella britovi ile enfekte olan olgularda MIF ve leptin düzeyleri araştırılmıştır. Otuzaltı akut trichinellosis (TT) olgu ve 20 sağlıklı kişi (K) çalışmaya dahil edildi. Hastalarda akut (TA) ve tedavi sonrası (TS) dönemlerde serum örneklerinde hs-CRP, MIF ve leptin düzeyleri ölçüldü, sağlıklı kişilerdeki serum düzeyleriyle de karşılaştırıldı. Hastalarda hs-CRP, lökosit, eozinofil ölçümleri akut enfeksiyon döneminde tedavi sonrası döneme göre yüksek bulundu (p<0.05). Leptin ve MIF değerleri akut trichinellosisli hastalarda kontrol grubuna göre daha yüksek saptandı. Kontrol ve olgu grupları arasındaki fark yalnızca leptin için istatistiksel olarak anlamlı bulundu. Trichinella varlığına bağlı olarak MİF ve leptin değerlerindeki bu artış hücresel immün yanıtın uyarılmasına bağlı olabileceği düşünüldü.

Anahtar Kelime: Leptin Trichinella Tedavi sonucu Trişinelloz Makrofaj migrasyon-inhibitör faktör Serum

Konular: Genel ve Dahili Tıp Parazitoloji

Akut trichinellosisli olgularda serum makrofaj migrasyon inhibitör faktör (MİF) ve leptin değerlerinin araştırılması

Öz:

The aim of this study was to examine the serum levels of MIF and leptin in patients infected with Trichinella britovi. Thirtysix patients with acute trichinellosis and 20 healthy control subjects were included in the study. Serum high sensitive C-reactive protein (hs-CRP), MIF and leptin levels were studied. Assessments were performed in the patients group during acute infection and after the treatment periods and we also compared these values with healthy controls. In the patients, hs-CRP, leukocyte and eosinophil counts, and the levels of muscle enzymes in the acute infectious period were found to be significantly higher than those in the post treatment period (p<0.05). Both leptin and MIF were higher in acute trichinellosis patients when compared to controls. However, this was only significant for leptin. This study indicates that leptin and MIF levels are increased in the sera of patients with acute trichinellosis.

Anahtar Kelime: Trichinellosis Macrophage Migration-Inhibitory Factors Serum Leptin Trichinella Treatment Outcome

Konular: Genel ve Dahili Tıp Parazitoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1) Akar S, Gurler O, Pozio E, Onen F, Sari I, Gerceker E, Gunes AJ, Akinci B, Birlik M, Akkoc N. 2007. Frequency and severity of musculoskeletal symptoms in humans during an outbreak of trichinellosis caused by Trichinella britovi. J Parasitol, 93: 341-344.
2) Bacher M, Metz CN, Calandra T, Mayer K, Chesney J, Lohoff M, Gemsa D, Donnelly T, Bucala R. 1996. An essential regulatory role for macrophage migration inhibitory factor in T-cell activation. Proc Natl Acad Sci U S A, 93: 7849-7854.
3) Bruschi F. 2002. The immune response to the parasitic nematode Trichinella and the ways to escape it. From experimental studies to implications for human infection. Curr Drug Targets Immune Endocr Metabol Disord, 2: 269-280.
4) Calandra T, Roger T. 2003. Macrophage migration inhibitory factor: a regulator of innate immunity. Nat Rev Immunol, 3: 791-800.
5) Calandra T, Bernhagen J, Mitchell RA, Bucala R. 1994. The macrophage is an important and previously unrecognized source of macrophage migration inhibitory factor. J Exp Med, 179: 1895-902.
6) Capo V, Despommier DD. 1996. Clinical aspects of infection with Trichinella spp. Clinical Microbiology Reviews, 9: 47-54.
7) La Cava A, Matarese G. 2004. The weight of leptin in immunity. Nat Rev Immunol, 4: 371-379.
8) Chaiyaroj SC, Rutta AS, Muenthaisong K, Watkins P, Na Ubol M, Looareesuwan S. 2004. Reduced levels of transforming growth factor-beta1, interleukin-12 and increased migration inhibitory factor are associated with severe malaria. Acta Trop, 89: 319-327.
9) van Crevel R, Karyadi E, Netea MG, Verhoef H, Nelwan RH, West CE, van der Meer JW. 2002. Decreased plasma leptin concentrations in tuberculosis patients are associated with wasting and inflammation. J Clin Endocrinol Metabol, 87: 758-63
10) Donn RP, Ray DW. 2004. Macrophage migration inhibitory factor: molecular, cellular and genetic aspects of a key neuroendocrine molecule. J Endocrinol, 182: 1-9
11) Dupouy-Camet J, Kociecka W, Bruschi F, Bolas-Fernandez F, Pozio E. 2002. Opinion on the diagnosis and treatment of human trichinellosis. Expert Opin Pharmacother, 3: 1117-30
12) Falcone FH, Loke P, Zang X, MacDonald AS, Maizels RM, Allen JE. 2001. A Brugia malayi homolog of macrophage migration inhibitory factor reveals an important link between macrophages and eosinophil recruitment during nematode infection. J Immunol, 167: 5348-5354.
13) Fantuzzi G, Faggioni R. 2000. Leptin in the regulation of immunity, inflammation, and hematopoiesis. J Leukoc Biol, 68: 437-46
14) Groove DI. 2000. Tissue Nematodes (Trichinosis, Dracunculiasis, Filariasis). In Principles and Practice of Infectious Diseases, ed. GL Mandell, JE Bennett, R Dolin, Philadelphia, Pa: Churchill Livingstone, Inc. pp. 2943-2949.
15) Haluzik M, Fiedler J, Nedvidkova J, Ceska R. 1999. Serum leptin concentrations in patients with combined hyperlipidemia: relationship to serum lipids and lipoproteins. Physiol Res, 48: 363-368.
16) Hultgren OH, Tarkowski A. 2001. Leptin in septic arthritis: decreased levels during infection and amelioration of disease activity upon its administration. Arthritis Res, 3: 389-394
17) Juttner S, Bernhagen J, Metz CN, Rollinghoff M, Bucala R, Gessner A. 1998. Migration inhibitory factor induces killing of Leishmania major by macrophages: dependence on reactive nitrogen intermediates and endogenous TNF-alpha. J Immunol, 161: 2383-2390.
18) Kozaci L, Ertug S, Kavak T, Okyay P, Chikanza I, Ertabaklar H. 2005. Kutanöz Leishmaniasisli Olgularda Serum Makrofaj Migrasyon İnhibitör Faktör (MİF) Değerlerinin Araştırılması. Turkiye Parazitol Derg, 29: 145-148.
19) Maruna P, Gurlich R, Frasko R, Haluzik M. 2001. Serum leptin levels in septic men correlate well with C-reactive protein (CRP) and TNF-alpha but not with BMI. Physiological Research, 50: 589-594.
20) Morales MA, Mele R, Sanchez M, Sacchini D, De Giacomo M, Pozio E. 2002. Increased CD8(+)-T-cell expression and a type 2 cytokine pattern during the muscular phase of Trichinella infection in humans. Infect Immun, 70: 233-239.
21) Murrell KD. 2000. Trichinosis. In Hunter's Tropical Medicine and Emerging Infectious Diseases, ed. GW Hunter, GT Strickland, Philadelphia: W. B. Saunders Company. pp. 781-787.
22) Nishihira J. 2000. Macrophage migration inhibitory factor (MIF): its essential role in the immune system and cell growth. J Interferon Cytokine Res, 20: 751-762.
23) Orita M, Yamamoto S, Katayama N, Fujita S. 2002. Macrophage migration inhibitory factor and the discovery of tautomerase inhibitors. Current Pharmaceutical Design, 8: 1297-1317.
24) Pulido-Mendez M, De Sanctis J, Rodriguez-Acosta A. 2002. Leptin and leptin receptors during malaria infection in mice. Folia Parasitol (Praha), 49: 249-251.
25) Roberts HC, Hardie LJ, Chappell LH, Mercer JG. 1999. Parasite-induced anorexia: leptin, insulin and corticosterone responses to infection with the nematode, Nippostrongylus brasiliensis. Parasitol, 118 (Pt 1): 117-123.
26) Rodriguez-Sosa M, Rosas LE, David JR, Bojalil R, Satoskar AR, Terrazas LI. 2003. Macrophage migration inhibitory factor plays a critical role in mediating protection against the helminth parasite Taenia crassiceps. Infect Immun, 71: 1247-1254.
27) Sarraf P, Frederich RC, Turner EM, Ma G, Jaskowiak NT, Rivet DJ, 3rd, Flier JS, Lowell BB, Fraker DL, Alexander HR. 1997. Multiple cytokines and acute inflammation raise mouse leptin levels: potential role in inflammatory anorexia. J Exp Med, 185: 171-175.
28) Stavitsky AB, Metz C, Liu S, Xianli J, Bucala R. 2003. Blockade of macrophage migration inhibitory factor (MIF) in Schistosoma japonicum-infected mice results in an increased adult worm burden and reduced fecundity. Parasite Immunol, 25: 369-374.
29) Talaat KR, Nutman TB. 2005. Parasitic Diseases. In Murray and Nadel's Textbook of Respiratory Medicine Online, ed. RJ Mason, VC Broaddus, JF Murray, JA Nadel, Saunders. pp. 1083-107:

APA	KOZACI L, Sari I, Akar S, Birlik M, Akkoç N, CHIKANZA I, ÖNEN F (2010). Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. , 156 - 160.
Chicago	KOZACI Leyla Didem,Sari Ismail,Akar Servet,Birlik Merih,Akkoç Nurullah,CHIKANZA Ian C.,ÖNEN Fatoş Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. (2010): 156 - 160.
MLA	KOZACI Leyla Didem,Sari Ismail,Akar Servet,Birlik Merih,Akkoç Nurullah,CHIKANZA Ian C.,ÖNEN Fatoş Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. , 2010, ss.156 - 160.
AMA	KOZACI L,Sari I,Akar S,Birlik M,Akkoç N,CHIKANZA I,ÖNEN F Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. . 2010; 156 - 160.
Vancouver	KOZACI L,Sari I,Akar S,Birlik M,Akkoç N,CHIKANZA I,ÖNEN F Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. . 2010; 156 - 160.
IEEE	KOZACI L,Sari I,Akar S,Birlik M,Akkoç N,CHIKANZA I,ÖNEN F "Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis." , ss.156 - 160, 2010.
ISNAD	KOZACI, Leyla Didem vd. "Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis". (2010), 156-160.

APA	KOZACI L, Sari I, Akar S, Birlik M, Akkoç N, CHIKANZA I, ÖNEN F (2010). Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. Türkiye Parazitoloji Dergisi, 34(3), 156 - 160.
Chicago	KOZACI Leyla Didem,Sari Ismail,Akar Servet,Birlik Merih,Akkoç Nurullah,CHIKANZA Ian C.,ÖNEN Fatoş Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. Türkiye Parazitoloji Dergisi 34, no.3 (2010): 156 - 160.
MLA	KOZACI Leyla Didem,Sari Ismail,Akar Servet,Birlik Merih,Akkoç Nurullah,CHIKANZA Ian C.,ÖNEN Fatoş Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. Türkiye Parazitoloji Dergisi, vol.34, no.3, 2010, ss.156 - 160.
AMA	KOZACI L,Sari I,Akar S,Birlik M,Akkoç N,CHIKANZA I,ÖNEN F Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. Türkiye Parazitoloji Dergisi. 2010; 34(3): 156 - 160.
Vancouver	KOZACI L,Sari I,Akar S,Birlik M,Akkoç N,CHIKANZA I,ÖNEN F Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis. Türkiye Parazitoloji Dergisi. 2010; 34(3): 156 - 160.
IEEE	KOZACI L,Sari I,Akar S,Birlik M,Akkoç N,CHIKANZA I,ÖNEN F "Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis." Türkiye Parazitoloji Dergisi, 34, ss.156 - 160, 2010.
ISNAD	KOZACI, Leyla Didem vd. "Serum levels of macrophage migration inhibitory factor and leptin in patients with acute trichinellosis". Türkiye Parazitoloji Dergisi 34/3 (2010), 156-160.