Yıl: 2010 Cilt: 26 Sayı: 2 Sayfa Aralığı: 69 - 73 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo)

Öz:
Gültiken ME, Yıldız D, Karahan S, Bolat D. Hindi (Meleagris gallopavo) Bursa fabricius’unun taramalı elektron ve ışık mikroskobu ile incelenmesi. Eurasian J Vet Sci, 2010, 26, 2, 69-73 Amaç: Hindide Bursa fabricius’un morfolojisinin belirli dönemlerde incelenmesi ve dönemlere göre morfometrik analizinin taramalı elektron mikroskobu ve ışık mikroskobu kullanılarak yapılmasıdır. Gereç ve Yöntem: Çalışmada 1-24 haftalığa kadar toplam 50 hindiye ait Bursa fabricius’lar kullanıldı. Hayvanların ve nekropsi sonrası Bursa fabricius’ların ağırlıkları belirlendi. Taramalı elektron mikroskobu ile yapılacak çalışma öncesi dokular gluteraldehit ile tespit edildikten sonra mikroskop kullanılarak görüntüler elde edildi. Histolojik çalışma için dokular rutin histolojik takip sonrası Mallory’s triple ve Haematoxylen-eosin ile boyanarak ışık mikroskobu ile incelendi. Bulgular: Morfometrik veriler Bursa fabricius’un maksimum büyüklüğüne 9. haftada ulaştığını gösterdi. Dokuzuncu haftayı takiben Bursa fabricius ağırlığındaki azalma hindide involusyonun bu dönemi takiben şekillenmeye başladığını gösterdi. Taramalı elektron mikroskop ile yapılan incelemelerde Bursa fabricius lumenine uzanan plica yüzeylerindeki kubbe şeklindeki epitel görüntüsünün 5. ve 9. haftalarda en belirgin ve düzgün olarak tespit edildi. İlerleyen haftalarda (13. ve 24.) Bu manzarada dikkat çekici bir düzensizlik gözlendi. Öneri: Çalışma sonucunda elde edilen bulguların, ilerde yapılacak çalışmalara ve hindi aşılama programlarına katkı yapacağı düşünülmektedir.
Anahtar Kelime: fabricius kesesi epitel ışık mikroskopisi humoral bağışıklık taramalı elektron mikroskopi Meleagris gallopavo hindi

Konular: Veterinerlik

Hindi (Meleagris gallopavo) Bursa fabricius’unun taramalı elektron ve ışık mikroskobu ile incelenmesi.

Öz:
Gultiken ME, Yildiz D, Karahan S, Bolat D. Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). Eurasian J Vet Sci, 2010, 26, 2, 69-73 Aim: The aim of this study was postnatal investigation of morphometric features of Bursa fabricius in Turkey by using scanning electron microscope and light microscope. Materials and Methods: One 1-24 week old 50 turkeys (meleagris gallopavo) were used. Their Bursa fabriciuses were taken out after the necropsy and weighted. Tissues were fixed with glutaraldehyde, examined and photographed under scanning electron microscope. For histological examination, tissues were prepared using routine histologic methods and stained with Mallory’s triple and Hematoxylen-Eosine. Results: The morphometric data concerning turkey ducklings used in the study showed that Bursa fabricius reaches its maximum size at the 9th week. The decrease in the weight of Bursa fabricius following the week of 9 proved that involution session in the turkey begins after that period. On the investigation performed by means of scanning electron microscopy, dome shaped surface epithelium which covers subepithelial lymph follicles on the surface was determined to be most clear in the 5th and 9th weeks. There was a distinct irregularity of this appearance in the following weeks ($13^ {th}$ and $24^ {th}$). Conclusion: It was concluded that the results may contribute to the researches which relate to humoral immunity formation and effectiveness of vaccination programme.
Anahtar Kelime: scanning electron microscopy Meleagris gallopavo turkeys bursa fabricii epithelium light microscopy humoral immunity

Konular: Veterinerlik
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Gültiken M, YILDIZ D, Karahan S, BOLAT D (2010). Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). , 69 - 73.
Chicago Gültiken Murat Erdem,YILDIZ Dinçer,Karahan Siyami,BOLAT DURMUŞ Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). (2010): 69 - 73.
MLA Gültiken Murat Erdem,YILDIZ Dinçer,Karahan Siyami,BOLAT DURMUŞ Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). , 2010, ss.69 - 73.
AMA Gültiken M,YILDIZ D,Karahan S,BOLAT D Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). . 2010; 69 - 73.
Vancouver Gültiken M,YILDIZ D,Karahan S,BOLAT D Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). . 2010; 69 - 73.
IEEE Gültiken M,YILDIZ D,Karahan S,BOLAT D "Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo)." , ss.69 - 73, 2010.
ISNAD Gültiken, Murat Erdem vd. "Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo)". (2010), 69-73.
APA Gültiken M, YILDIZ D, Karahan S, BOLAT D (2010). Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). Eurasian Journal of Veterinary Sciences, 26(2), 69 - 73.
Chicago Gültiken Murat Erdem,YILDIZ Dinçer,Karahan Siyami,BOLAT DURMUŞ Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). Eurasian Journal of Veterinary Sciences 26, no.2 (2010): 69 - 73.
MLA Gültiken Murat Erdem,YILDIZ Dinçer,Karahan Siyami,BOLAT DURMUŞ Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). Eurasian Journal of Veterinary Sciences, vol.26, no.2, 2010, ss.69 - 73.
AMA Gültiken M,YILDIZ D,Karahan S,BOLAT D Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). Eurasian Journal of Veterinary Sciences. 2010; 26(2): 69 - 73.
Vancouver Gültiken M,YILDIZ D,Karahan S,BOLAT D Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo). Eurasian Journal of Veterinary Sciences. 2010; 26(2): 69 - 73.
IEEE Gültiken M,YILDIZ D,Karahan S,BOLAT D "Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo)." Eurasian Journal of Veterinary Sciences, 26, ss.69 - 73, 2010.
ISNAD Gültiken, Murat Erdem vd. "Scanning electron and light microscopic investigation of Bursa fabricius in turkey (Meleagris gallopavo)". Eurasian Journal of Veterinary Sciences 26/2 (2010), 69-73.