Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey

Erdem, Belgin; KARİPTAŞ, ERGİN; Çil, Elif; Işık, Kamil

Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey

Belgin ERDEM, (Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırşehir, Türkiye)

Ergin KARİPTAŞ, (Ahi Evran Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Kırşehir, Türkiye)

Elif ÇİL, (Ondokuz Mayıs Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Samsun, Türkiye)

Kamil IŞIK (Ondokuz Mayıs Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Samsun, Türkiye)

Turkish Journal of Biology

3 0

Yıl: 2011 Cilt: 35 Sayı: 4 Sayfa Aralığı: 463 - 472 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey

Öz:

Kırmızı et, çiğ tavuk, kıyma ve balıktan alınan Aeromonas suşlarına ait yeni 126 izolatın fenotipik verilerini analiz etmek için nümerik taksonomi kullanılmıştır. Her bir suş 86 karakter bakımından test edilmesine rağmen son veri seti 63 karakter ile SSM benzerlik katsayısı ve UPGMA kümeleme algoritmasıyla analiz edilmiştir. Suşlar % 83’den büyük SSM değerlerinde, 7 büyük (5 ve üzeri suş), 3 küçük (2-4 suş) ve 5 adet tek üyeli kümeden oluşan 10 agregat grup içinde toplanmış ve bu suşlar sırasıyla A. hydrophila, A. caviae ve A. sobria olarak tanımlanmışlardır. Gıdalardan elde edilen izolatların nisbi bir fenotipik aralık gösterdiği ve suş gruplarının mevcut tanımlama şemasındaki tip örnekleriyle mukayese edildiklerinde tipik olmayan profi llere sahip olduğu kanıtlanmıştır. Fenetik yaklaşımın Aeromonas türlerini tanımlamada ve sınırlandırmada gerekli bir araç olduğu açıkça görülmüştür. Farklı kaynaklardan izole edilen Aeromonas suşlarının nümerik taksonomisi, özellikle gıdalarda potansiyel patojenik Aeromonas’ların varlığını ortaya çıkarmıştır

Anahtar Kelime: çiğ gıdalar Aeromonas kemotaksonomi numerik taksonomi bakteriler et çiğ balık tavuk eti

Konular: Biyoloji

Türkiye’de gıda örneklerinden izole edilen Aeromonasların biyokimyasal tanımlanması ve nümerik taksonomisi

Öz:

Numerical taxonomy was used to analyze phenotypic data obtained from 126 new isolates of Aeromonas strains taken from red meat, raw chicken, minced meat, and fi sh samples. Each strain was tested for 86 characters but only the fi nal data including 63 characters were analysed using the SSM coeffi cients and the UPGMA clustering algorithm. At SSM values of ≥ 83%, the strains clustered into 10 aggregate groups consisting of 7 major (5 and up strains) and 3 minor (2-4 strains), and 5 single member clusters, each of which was identifi ed as A. hydrophila, A. caviae, and A. sobria, respectively. It was proved that the food isolates showed a relative phenotypical distance and the groups of strains that had atypical profi les were compared with the type species by the present identifi cation schemes. It was clearly seen that the phenetic approach was a necessary tool to delimitate and identify the Aeromonas species. Numerical taxonomy of Aeromonas strains isolated from diff erent sources revealed the presence of potentially pathogenic Aeromonas spp., especially in food.

Anahtar Kelime: numerical taxonomy bacteria meat raw fish chicken meat raw foods Aeromonas chemotaxonomy

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Erdem B, KARİPTAŞ E, Çil E, Işık K (2011). Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. , 463 - 472.
Chicago	Erdem Belgin,KARİPTAŞ ERGİN,Çil Elif,Işık Kamil Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. (2011): 463 - 472.
MLA	Erdem Belgin,KARİPTAŞ ERGİN,Çil Elif,Işık Kamil Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. , 2011, ss.463 - 472.
AMA	Erdem B,KARİPTAŞ E,Çil E,Işık K Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. . 2011; 463 - 472.
Vancouver	Erdem B,KARİPTAŞ E,Çil E,Işık K Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. . 2011; 463 - 472.
IEEE	Erdem B,KARİPTAŞ E,Çil E,Işık K "Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey." , ss.463 - 472, 2011.
ISNAD	Erdem, Belgin vd. "Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey". (2011), 463-472.

APA	Erdem B, KARİPTAŞ E, Çil E, Işık K (2011). Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. Turkish Journal of Biology, 35(4), 463 - 472.
Chicago	Erdem Belgin,KARİPTAŞ ERGİN,Çil Elif,Işık Kamil Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. Turkish Journal of Biology 35, no.4 (2011): 463 - 472.
MLA	Erdem Belgin,KARİPTAŞ ERGİN,Çil Elif,Işık Kamil Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. Turkish Journal of Biology, vol.35, no.4, 2011, ss.463 - 472.
AMA	Erdem B,KARİPTAŞ E,Çil E,Işık K Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. Turkish Journal of Biology. 2011; 35(4): 463 - 472.
Vancouver	Erdem B,KARİPTAŞ E,Çil E,Işık K Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey. Turkish Journal of Biology. 2011; 35(4): 463 - 472.
IEEE	Erdem B,KARİPTAŞ E,Çil E,Işık K "Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey." Turkish Journal of Biology, 35, ss.463 - 472, 2011.
ISNAD	Erdem, Belgin vd. "Biochemical identification and numerical taxonomy of Aeromonas spp. isolated from food samples in Turkey". Turkish Journal of Biology 35/4 (2011), 463-472.