Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains

ÇAKIR, ERDOĞAN; KIVANÇ, Merih; YILMAZ CANKILIÇ, MERAL

Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains

Merih KIVANÇ, (Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

MERAL YILMAZ CANKILIÇ, (Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Erdoğan ÇAKIR (Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

Turkish Journal of Biology

5 0

Yıl: 2011 Cilt: 35 Sayı: 3 Sayfa Aralığı: 313 - 324 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains

Öz:

Bu çalışmada Türkiye’deki boza örneklerinden toplam 45 adet laktik asit bakterisi izole edilmiştir. Bu izolatlarkoloni yapıları, hücre morfolojileri ve API CHL50 (Biomérieux) kit sistemine dayalı olarak tanımlanmışlardır. Ayrıcaotomatik RiboPrinter® Mikrobiyal Karakterizasyon Sistemi (DuPont Qualicon) de identii kasyon için kullanılmıştır. Buizolatlar gıda kaynaklı bakteriyel patojenlere karşı inhibitor aktivitesi bakımından test edilmiştir. Laktik asit bakterilerininantimikrobiyal etkisi agar spot test ve agar difüzyon metodu ile belirlenmiştir. Antimikrobiyal aktiviteye sahip izolatlar(45 izolat) RiboPrinter® Mikrobiyal Karakterizasyon Sistemi (DuPont Qualicon)’ne göre Lactococcus lactis subsp. lactis(2 izolat), Leuconostoc citreum (5 izolat), Lactobacillus brevis (4 izolat), Lactobacillus plantarum (24 izolat), Lactobacillusparaplantarum (1 izolat), Enterococcus faecium (1 izolat), Lactobacillus graminis (4 izolat), Pediococcus species (1 izolat)ve Lactobacillus paracasei subsp. paracasei (3 izolat) olarak tanımlanmışlardır. Ayrıca laktik asit bakterileri tarafındanüretilen laktik asit miktarı Y-12 izolatı ile elde edilen minimum 0.16 mg/mL ve maksimum KB13 izolatı ile elde edilen7.79 mg/mL’dir. Hidrojen peroksit miktarları Z12 izolatı ile elde edilen minimum 0.69 μg/mL ve maksimum X-13 izolatıile elde edilen 2.18 μg/mL’dir. Proteolitik aktivite miktarları ise minimum Y-12 izolatı ile elde edilen 0.04 mg/mL vemaksimum KB13 izolatı ile elde edilen 2.57 mg/mL’dir.

Anahtar Kelime: proteolitik bakteri boza laktik asit bakterileri tanı bakteriler karakterizasyon izolasyon

Konular: Biyoloji

Bozadan izole edilen laktik asit bakterilerinin izolasyonu, identifikasyonu ve bazı test mikroorganizmalarına karşı mikrobiyal aktivitesi

Öz:

A total of 45 lactic acid bacteria were isolated from 10 different boza samples consumed in Turkey. Theisolates were identified with respect to colonial, cellular morphology, and biochemical characters based on API CHL50(Biomérieux) kit and also automated riboprinting was used to identify of the isolates. These isolates were tested forinhibitory activity against food-borne bacterial pathogens. Antimicrobial effects of these lactic acid bacteria weredetermined by the agar spot test and also agar diffusion method. As a result of the RiboPrinter® Microbial CharacterizationSystem (DuPont Qualicon) lactic acid bacteria isolates having antimicrobial activity (45 isolates) were identified asLactococcus lactis subsp. lactis (2 isolates), Leuconostoc citreum (5 isolates), Lactobacillus brevis (4 isolates), Lactobacillusplantarum (24 isolates), Lactobacillus paraplantarum (1 isolate), Enterococcus faecium (1 isolate), Lactobacillus graminis(4 isolates), Pediococcus species (1 isolate), and Lactobacillus paracasei subsp. paracasei (3 isolates). In addition, theamount of lactic acid produced by lactic acid bacteria was minimum 0.16 mg/mL with Y-12 isolate and maximum 7.79mg/mL with KB13 isolate, hydrogen peroxide minimum 0.69 μg/mL with Z-12 isolate and maximum 2.18 μg/mL withX-13 isolate, and proteolytic activity 0.04 mg/mL with Y-12 isolate and maximum 2.57 mg/mL with KB13 isolate.

Anahtar Kelime: bacteria characterization isolation proteolytic bacteria boza lactic acid bacteria identification

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KIVANÇ M, YILMAZ CANKILIÇ M, ÇAKIR E (2011). Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. , 313 - 324.
Chicago	KIVANÇ Merih,YILMAZ CANKILIÇ MERAL,ÇAKIR ERDOĞAN Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. (2011): 313 - 324.
MLA	KIVANÇ Merih,YILMAZ CANKILIÇ MERAL,ÇAKIR ERDOĞAN Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. , 2011, ss.313 - 324.
AMA	KIVANÇ M,YILMAZ CANKILIÇ M,ÇAKIR E Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. . 2011; 313 - 324.
Vancouver	KIVANÇ M,YILMAZ CANKILIÇ M,ÇAKIR E Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. . 2011; 313 - 324.
IEEE	KIVANÇ M,YILMAZ CANKILIÇ M,ÇAKIR E "Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains." , ss.313 - 324, 2011.
ISNAD	KIVANÇ, Merih vd. "Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains". (2011), 313-324.

APA	KIVANÇ M, YILMAZ CANKILIÇ M, ÇAKIR E (2011). Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turkish Journal of Biology, 35(3), 313 - 324.
Chicago	KIVANÇ Merih,YILMAZ CANKILIÇ MERAL,ÇAKIR ERDOĞAN Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turkish Journal of Biology 35, no.3 (2011): 313 - 324.
MLA	KIVANÇ Merih,YILMAZ CANKILIÇ MERAL,ÇAKIR ERDOĞAN Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turkish Journal of Biology, vol.35, no.3, 2011, ss.313 - 324.
AMA	KIVANÇ M,YILMAZ CANKILIÇ M,ÇAKIR E Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turkish Journal of Biology. 2011; 35(3): 313 - 324.
Vancouver	KIVANÇ M,YILMAZ CANKILIÇ M,ÇAKIR E Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains. Turkish Journal of Biology. 2011; 35(3): 313 - 324.
IEEE	KIVANÇ M,YILMAZ CANKILIÇ M,ÇAKIR E "Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains." Turkish Journal of Biology, 35, ss.313 - 324, 2011.
ISNAD	KIVANÇ, Merih vd. "Isolation and identification of lactic acid bacteria from boza, and their microbial activity against several reporter strains". Turkish Journal of Biology 35/3 (2011), 313-324.