Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle
Yıl: 2011 Cilt: 17 Sayı: 5 Sayfa Aralığı: 795 - 802 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle
Öz: Ovaryum steroid hormonları, özellikle östrojen ve progesteron, ovaryum fonksiyonlarının düzenlenmesinde merkezi bir rol oynar. Progesteron (PR) ve östrojen (ER)’in biyolojik etkileri hem PR hem de ER izoformlarının aktivasyonuna bağlıdır. Bu çalışmada, seksüel siklusun foliküler ve luteal fazı boyunca inek ovaryumunda progesteron reseptör B (PR-B) ve östrojen reseptör alfa (ERα)’nın lokalizasyonunu ve hücresel dağılımını immunohistokimyasal yöntemler kullanarak çalıştık. Yirmiüç Holstein ineğin östrus siklus dönemi, ovaryumun bütünü ve histolojik görünümü ve kan steroid hormon değerleri ile değerlendirildi. Ovaryumdan elde edilen doku örnekleri rutin histolojik işlemler için %10’luk formaldehit içinde tespit edildi. ERα immunoreaktivitesi, korpus albikans hücrelerinde, korpus luteum hücrelerinde, teka hücrelerinde, bağ doku hücrelerinde, germinal epitel hücrelerinde ve primer folikülden olgun foliküle her aşamada gelişen foliküllerin granuloza hücre çekirdeklerinde gözlendi. PR-B için çekirdek boyanma, tüm büyüklükteki foliküllerin granuloza hücrelerinde saptanamadı, ancak korpus luteum hücrelerinde, korpus albikans hücrelerinde, bağ doku hücrelerinde ve germinal epitel hücrelerinde pozitif boyanma görüldü. Sonuç olarak, inek ovaryumunda ERα ve PR-B immunoreaktivitesi açısından seksüel siklusun fazları arasında çok fazla fark olmadığı ancak hücre-spesifik lokalizasyonda önemli farklılıkların olduğu gösterildi.
Anahtar Kelime: Konular:
İnek Ovaryumunda Seksüel Siklusun Foliküler ve Luteal Fazı Boyunca Östrojen Reseptör Alfa ve Progesteron Reseptör B’nin Lokalizasyonu
Öz: Ovarian steroid hormones, mainly estrogen and progesterone, play a central role in the regulation of ovarian functions. The biological effects of progesterone (PR) and estrogen (ER) are dependent on the activation of both PR and ER isoforms. In the present study, we studied the cellular distribution and localization of progesterone receptor B (PR-B) and estrogen receptor alpha (ERα) in the bovine ovaries during the follicular and luteal phases of the sexual cycle using immunohistochemical methods. The estrous cycle stage of 23 Holstein bovines was evaluated by gross and histological appearance of ovaries and blood steroid hormone values. Tissue samples from ovaries were fixed in 10% formaldehyde for routine histological processing. ERα immunoreactivity was observed in nuclei of granulosa cells of growing follicles at all stages from primary to mature follicles, epithelial cells of the germinal epithelium, stroma cells, theca cells, corpora lutea cells and cells of the corpora albicantia. Nuclear staining for PR-B was not detected in the granulosa cells in all sizes of follicles, but was positively stained in germinal epithelium cells, stroma cells, corpora albicantia cells and corpora lutea cells. In conclusion, ERα and PR-B immunoreactivity in bovine ovary were not indicate too much difference between the phases of the sexual cycle but have important differences in cell-spesific localization.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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| APA | AKBALIK M, SAĞSÖZ H, SARUHAN G (2011). Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. , 795 - 802. |
| Chicago | AKBALIK Mehmet Erdem,SAĞSÖZ Hakan,SARUHAN Güney Berna Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. (2011): 795 - 802. |
| MLA | AKBALIK Mehmet Erdem,SAĞSÖZ Hakan,SARUHAN Güney Berna Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. , 2011, ss.795 - 802. |
| AMA | AKBALIK M,SAĞSÖZ H,SARUHAN G Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. . 2011; 795 - 802. |
| Vancouver | AKBALIK M,SAĞSÖZ H,SARUHAN G Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. . 2011; 795 - 802. |
| IEEE | AKBALIK M,SAĞSÖZ H,SARUHAN G "Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle." , ss.795 - 802, 2011. |
| ISNAD | AKBALIK, Mehmet Erdem vd. "Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle". (2011), 795-802. |
| APA | AKBALIK M, SAĞSÖZ H, SARUHAN G (2011). Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(5), 795 - 802. |
| Chicago | AKBALIK Mehmet Erdem,SAĞSÖZ Hakan,SARUHAN Güney Berna Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17, no.5 (2011): 795 - 802. |
| MLA | AKBALIK Mehmet Erdem,SAĞSÖZ Hakan,SARUHAN Güney Berna Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.17, no.5, 2011, ss.795 - 802. |
| AMA | AKBALIK M,SAĞSÖZ H,SARUHAN G Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(5): 795 - 802. |
| Vancouver | AKBALIK M,SAĞSÖZ H,SARUHAN G Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2011; 17(5): 795 - 802. |
| IEEE | AKBALIK M,SAĞSÖZ H,SARUHAN G "Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17, ss.795 - 802, 2011. |
| ISNAD | AKBALIK, Mehmet Erdem vd. "Localization of Estrogen Receptor Alpha and Progesterone Receptor B in the Bovine Ovary During the Follicular and Luteal Phase of the Sexual Cycle". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 17/5 (2011), 795-802. |
