The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression

Kaya, Mehmet Demir; SAĞLAM, Günseli; BİLTEKİN, BURCU; BARAK, Tanyeli; İZGİ, Nail; KARASU, Aykut; aras, yavuz; Sabancı, Pulat Akın; HEPGÜL, Kemal Tanju; BILIR, AYHAN

The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression

Aykut KARASU, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Yavuz ARAS, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Pulat Akın SABANCI, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Günseli SAĞLAM, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Anesteziyoloji Anabilim Dalı, İstanbul, Türkiye)

Nail İZGİ, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Burcu BİLTEKİN, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İstanbul, Türkiye)

Tanyeli BARAK, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Kemal Tanju HEPGÜL, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Nöroşirürji Anabilim Dalı, İstanbul, Türkiye)

Mehmet KAYA, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Fizyoloji Anabilim Dalı, İstanbul, Türkiye)

Ayhan BİLİR (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İstanbul, Türkiye)

Ulusal Travma ve Acil Cerrahi Dergisi

1 1

Yıl: 2010 Cilt: 16 Sayı: 5 Sayfa Aralığı: 390 - 394 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression

Öz:

AMAÇ Aquaporin-4 (AQP4) merkezi sinir sistemindeki başlıca su kanalıdır. Beyin ödemi travmatik beyin yaralanmasında (TBY) artmış AQP4 ekspresyonundan ortaya çıkmaktadır. Hücre çalışmaları protein kinaz aktivatörü forbol esterin AQP-4 ve su geçirgenliği üzerine baskılayıcı etkisi olduğunu göstermiştir. Bu çalışmanın amacı, bir forbol esteri olan forbol dibütiratın (PDBu) TBY’de AQP-4 ekspresyonu ve eşlik eden beyin ödemine etkisinin araştırılmasıdır. GEREÇ VE YÖNTEM Elli altı adet erkek Wistar cinsi sıçan öncelikle beyin dokusundaki su yüzdesinin değerlendirileceği ödem grubu ve AQP-4 ekspresyonunun araştırılacağı immünohistokimya grubu olmak üzere 2 gruba ayrıldı. Bu iki grup da kendi içerisinde kontrol grubu, ciddi TBY grubu, %0,9’luk tuz çözeltisi ile tedavi edilen TBY grubu ve PDBu (2300 mg/kg, iv) ile tedavi edilen TBY grubu olmak üzere 4 ayrı alt gruba ayrıldı. Sonuçlar istatistiksel olarak değerlendirildi. BULGULAR PDBu tedavisi anlamlı derecede beyin su konsantrasyonunu düşürdü (p<0,001). Ayrıca PDBu’nun travmaya bağlı AQP-4 reseptörlerinin artışını engellendiği tespit edildi (p<0,05). SONUÇ AQP-4 reseptörlerini azaltma etkisi oluşturan intravenöz PDBu verilmesiyle travmatik beyin ödemi gelişmesi engellenmiştir. Ayrıca bu durum TBY’da AQP-4 ekspresyonunun önemini de vurgulamaktadır.

Anahtar Kelime:

Konular: Cerrahi

Protein kinaz C aktivatörü forbol dibütiratın travmatik beyin ödemine ve aquaporin 4 ekspresyonu üzerine etkisi

Öz:

BACKGROUND Aquaporin-4 (AQP4) is the major water channel in the central nervous system. Brain edema emerges from increased AQP4 expression in traumatic brain injury (TBI). Cell line studies have shown that the protein kinase activator phorbol ester exerts a suppressive effect on AQP4 and water permeability. The aim of this study was to investigate the effects of a phorbol ester, phorbol dibutyrate (PDBu), on increased TBI AQP4 expression and accompanying brain edema. METHODS Fifty-six male Wistar rats were first divided into two groups: the edema group, in which the percentage of water in brain tissue would be evaluated, and the immunohistochemical group, allowing AQP4 expression to be determined. Both groups were further sub-divided into four groups consisting of 7 subjects. These four groups were as follows: sham-operated control group, severe diffuse TBI group, 0.9% saline-treated diffuse TBI group, and the PDBu-treated diffuse TBI group (2300 µg/kg, iv). The results were evaluated statistically. RESULTS PDBu treatment significantly reduced brain water concentration (p<0.001). Furthermore, PDBu was found to reduce trauma-induced AQP4 upregulation (p<0.05). CONCLUSION This study showed that traumatic brain edema was prevented by intravenous PDBu administration via AQP4 downregulation, supporting the idea emphasizing the importance of AQP4 expression control in TBI.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	KARASU A, aras y, Sabancı P, SAĞLAM G, İZGİ N, BİLTEKİN B, BARAK T, HEPGÜL K, Kaya M, BILIR A (2010). The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. , 390 - 394.
Chicago	KARASU Aykut,aras yavuz,Sabancı Pulat Akın,SAĞLAM Günseli,İZGİ Nail,BİLTEKİN BURCU,BARAK Tanyeli,HEPGÜL Kemal Tanju,Kaya Mehmet Demir,BILIR AYHAN The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. (2010): 390 - 394.
MLA	KARASU Aykut,aras yavuz,Sabancı Pulat Akın,SAĞLAM Günseli,İZGİ Nail,BİLTEKİN BURCU,BARAK Tanyeli,HEPGÜL Kemal Tanju,Kaya Mehmet Demir,BILIR AYHAN The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. , 2010, ss.390 - 394.
AMA	KARASU A,aras y,Sabancı P,SAĞLAM G,İZGİ N,BİLTEKİN B,BARAK T,HEPGÜL K,Kaya M,BILIR A The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. . 2010; 390 - 394.
Vancouver	KARASU A,aras y,Sabancı P,SAĞLAM G,İZGİ N,BİLTEKİN B,BARAK T,HEPGÜL K,Kaya M,BILIR A The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. . 2010; 390 - 394.
IEEE	KARASU A,aras y,Sabancı P,SAĞLAM G,İZGİ N,BİLTEKİN B,BARAK T,HEPGÜL K,Kaya M,BILIR A "The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression." , ss.390 - 394, 2010.
ISNAD	KARASU, Aykut vd. "The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression". (2010), 390-394.

APA	KARASU A, aras y, Sabancı P, SAĞLAM G, İZGİ N, BİLTEKİN B, BARAK T, HEPGÜL K, Kaya M, BILIR A (2010). The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. Ulusal Travma ve Acil Cerrahi Dergisi, 16(5), 390 - 394.
Chicago	KARASU Aykut,aras yavuz,Sabancı Pulat Akın,SAĞLAM Günseli,İZGİ Nail,BİLTEKİN BURCU,BARAK Tanyeli,HEPGÜL Kemal Tanju,Kaya Mehmet Demir,BILIR AYHAN The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. Ulusal Travma ve Acil Cerrahi Dergisi 16, no.5 (2010): 390 - 394.
MLA	KARASU Aykut,aras yavuz,Sabancı Pulat Akın,SAĞLAM Günseli,İZGİ Nail,BİLTEKİN BURCU,BARAK Tanyeli,HEPGÜL Kemal Tanju,Kaya Mehmet Demir,BILIR AYHAN The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. Ulusal Travma ve Acil Cerrahi Dergisi, vol.16, no.5, 2010, ss.390 - 394.
AMA	KARASU A,aras y,Sabancı P,SAĞLAM G,İZGİ N,BİLTEKİN B,BARAK T,HEPGÜL K,Kaya M,BILIR A The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. Ulusal Travma ve Acil Cerrahi Dergisi. 2010; 16(5): 390 - 394.
Vancouver	KARASU A,aras y,Sabancı P,SAĞLAM G,İZGİ N,BİLTEKİN B,BARAK T,HEPGÜL K,Kaya M,BILIR A The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression. Ulusal Travma ve Acil Cerrahi Dergisi. 2010; 16(5): 390 - 394.
IEEE	KARASU A,aras y,Sabancı P,SAĞLAM G,İZGİ N,BİLTEKİN B,BARAK T,HEPGÜL K,Kaya M,BILIR A "The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression." Ulusal Travma ve Acil Cerrahi Dergisi, 16, ss.390 - 394, 2010.
ISNAD	KARASU, Aykut vd. "The effects of protein kinase C activator phorbol dibutyrate on traumatic brain edema and aquaporin-4 expression". Ulusal Travma ve Acil Cerrahi Dergisi 16/5 (2010), 390-394.