Yıl: 2011 Cilt: 35 Sayı: 1 Sayfa Aralığı: 35 - 44 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar

Öz:
Lipazlar (triaçilgliserol hidrolazlar), yağ-su arayüzeyinde, uzun zincirli açilgliserollerin, ester bağlarının hidrolizini katalizleyen hidrolitik enzimlerdir. Bu çalışmada, Rhizopus delemar’ın lipaz aktivitesi üzerine, fermentasyon ortamındaki indükleyicilerin, yüzey aktif maddelerin, aktivatörler ve inhibitörlerin etkileri araştırıldı. İndükleyici olarak, belirli ticari yağlar ve tribütirinin varlığında, elde edilen lipaz aktiviteleri, sırasıyla ayçiçeği yağı > soya yağı > fındık yağı > mısır yağı > tribütirin > zeytin yağı olarak azalmaktadır. Buna ek olarak, karıştırma hızı ve havalandırma hızının lipaz aktivitesi üzerine etkileri araştırıldı. Yüzey aktif maddelerin lipaz aktivitesine etkisinin incelenmesi için, iki farklı yüzey aktif madde, Tween-80 ve rhamnolipid (biyosurfaktan), tribütirin ile karşılaştırmalı olarak kullanılmıştır. Maksimum lipaz aktivitesi, hem bir indükleyici hem de bir yüzey aktif madde olarak Tween-80 içeren fermantasyon ortamında elde edilmiştir. R. delemar’ la lipaz üretimi üzerine, melas ve sakkarozun birleştirilmiş etkileri de araştırılmıştır. Aktivatörlerin varlığında elde edilen lipaz aktiviteleri, sırasıyla NaCl > KCl > $CaCl _2 > MgCl _2$ > arap zamkı > EDTA olarak azalmış ve maksimum lipaz aktivitesi 964,55 U/L olarak bulunmuştur. Fermantasyon ortamında bulunan ağır metal iyonları lipaz aktivitesini şiddetli bir şekilde düşürmüştür. Ağır metal iyonlarının varlığında lipaz aktivitesi sırasıyla $Fe^ {+2}>Mn^ {+2} >Co^ {+2}>Ni^ {+2}$ olarak azalmıştır.
Anahtar Kelime: kesikli kültür fermentasyon ağır metaller kültür ortamı triasilgliserol lipaz enzim aktivitesi lipaz biyoreaktörler Rhizopus delemar üretim Rhizopus

Konular: Biyoloji

Üreme ortamı bileşimi ve fermentasyon koşullarının R. delemar’ın lipaz üretimi üzerine etkileri

Öz:
Lipases (triacylglycerol hydrolases) are hydrolytic enzymes that can catalyze the hydrolysis of the ester bond of long-chain acylglycerols at the oil-water interface. The present study investigated the effects of inducers, surface-active materials, activators, and inhibitors in the fermentation medium on lipase activity in Rhizopus delemar. In the presence of certain commercial oils and tributyrin as an inducer, lipase activity decreased in the order of sunflower oil > soybean oil > hazelnut oil > corn oil > tributyrin > olive oil. In addition, the effects of the stirring and aeration rates on lipase activity were investigated. To investigate the effect of surface-active materials on lipase activity 2 different surface-active materials, Tween-80 and rhamnolipid (biosurfactant), were used as comparatives with tributyrin. Maximum lipase activity was observed in the fermentation medium containing Tween-80 as both an inducer and surface-active material. The combined effects of molasses and sucrose on lipase production by R. delemar were also investigated. Lipase activity in the presence of activators decreased in the order of NaCl > KCl > $CaCl _2 > MgCl _2$ > gum arabic > EDTA, and maximum lipase activity of 964.55 U/L was obtained. The presence of heavy metal ions in the fermentation media severely inhibited lipase activity. Lipase activity in the presence of heavy metal ions decreased in the order of $Fe^ {+2}>Mn^ {+2} >Co^ {+2}>Ni^ {+2}$.
Anahtar Kelime: triacylglycerol lipase enzyme activity lipase bioreactors Rhizopus delemar production Rhizopus batch culture fermentation heavy metals culture media

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
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APA Açıkel Ü, ERŞAN M, SAĞ AÇIKEL Y (2011). The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. , 35 - 44.
Chicago Açıkel Ünsal,ERŞAN MEHTAP,SAĞ AÇIKEL YEŞİM The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. (2011): 35 - 44.
MLA Açıkel Ünsal,ERŞAN MEHTAP,SAĞ AÇIKEL YEŞİM The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. , 2011, ss.35 - 44.
AMA Açıkel Ü,ERŞAN M,SAĞ AÇIKEL Y The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. . 2011; 35 - 44.
Vancouver Açıkel Ü,ERŞAN M,SAĞ AÇIKEL Y The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. . 2011; 35 - 44.
IEEE Açıkel Ü,ERŞAN M,SAĞ AÇIKEL Y "The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar." , ss.35 - 44, 2011.
ISNAD Açıkel, Ünsal vd. "The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar". (2011), 35-44.
APA Açıkel Ü, ERŞAN M, SAĞ AÇIKEL Y (2011). The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. Turkish Journal of Biology, 35(1), 35 - 44.
Chicago Açıkel Ünsal,ERŞAN MEHTAP,SAĞ AÇIKEL YEŞİM The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. Turkish Journal of Biology 35, no.1 (2011): 35 - 44.
MLA Açıkel Ünsal,ERŞAN MEHTAP,SAĞ AÇIKEL YEŞİM The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. Turkish Journal of Biology, vol.35, no.1, 2011, ss.35 - 44.
AMA Açıkel Ü,ERŞAN M,SAĞ AÇIKEL Y The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. Turkish Journal of Biology. 2011; 35(1): 35 - 44.
Vancouver Açıkel Ü,ERŞAN M,SAĞ AÇIKEL Y The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar. Turkish Journal of Biology. 2011; 35(1): 35 - 44.
IEEE Açıkel Ü,ERŞAN M,SAĞ AÇIKEL Y "The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar." Turkish Journal of Biology, 35, ss.35 - 44, 2011.
ISNAD Açıkel, Ünsal vd. "The effects of the composition of growth medium and fermentation conditions on the production of lipase by R. delemar". Turkish Journal of Biology 35/1 (2011), 35-44.