Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation

DİNLER, Kahraman; KUKNER, Aysel; BÜYÜKAŞIK, Oktay; ÇÖL, Cavit; Fırat, Tülin; HASDEMİR, Ahmet Oğuz

Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation

Cavit ÇÖL, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Bolu, Türkiye)

Kahraman DİNLER, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Bolu, Türkiye)

Ahmet Oğuz HASDEMİR, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Bolu, Türkiye)

Oktay BÜYÜKAŞIK, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Bolu, Türkiye)

Tülin FIRAT, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bolu, Türkiye)

Aysel KÜKNER (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bolu, Türkiye)

Turkish Journal of Gastroenterology

2 0

Yıl: 2010 Cilt: 21 Sayı: 4 Sayfa Aralığı: 433 - 438 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation

Öz:

Amaç: Bu çalışma obstruktif akut pankreatit modelinde intraperitoneal olarak enjekte edilen ekzojen melatoninin histopatolojik değişiklikler üzerindeki koruyucu etkisini incelemek amacıyla gerçekleştirilmiştir. Yöntem: Bu çalışmada 20 erişkin Winstar Albino rat kullanılarak obstruktif akut pankreatit ve pankreatik atrofi modeli için pankreatikobilier kanal ligasyon tekniği uygulanmıştır. Bir grup rat'ta bir hafta süreyle intraperitoneal olarak 20 mg/kg melatonin solusyonu enjekte edildi ve pankreas dokusundaki histopatolojik değişiklikler yönünden kontrol grubu ratlarla karşılaştırıldı. Bulgular: Apoptozis görülme oranı kontrol grubunda %30.5 olduğu halde çalışma grubunda %12.7 bulunmuştur. Pankreatik ödem, kanama ve polimorf nükleer lökosit infiltrasyonu da kontrol grubuna gore melatonin grubunda daha az görülmüştür. Sonuç: Obstruktif akut pankreatitli ratlarda 7 gün süreyle ekzojen melatonin enjeksiyonu asiner hücre dejenerasyonu, apoptosis ve polimorf nükleer hücre infiltrasyonunu azaltmaktadır.

Anahtar Kelime:

Konular: Cerrahi

Duktal ligasyon tekniğiyle oluşturulmuş akut pankreatitli sıçanlarda intraperitoneal melatonin enjeksiyonunun etkisinin değerlendirilmesi

Öz:

Background/aims: The aim of this study was to address the protective effects of exogenous melatonin injections intraperitoneally on the histopathological changes in a model of obstructive acute pancreatitis. Methods: In this study, ductal ligation technique was used in 20 adult male Wistar Albino rats to develop a model of obstructive acute pancreatitis and beginning pancreatic atrophy. Melatonin 20 mg/kg solution was injected in one group of rats intraperitoneally for one week and results were compared with the control group regarding histopathological findings in the pancreatic tissue. Results: The apoptosis rate in control group rats was 30.5%, while it was 12.7% in the melatonin group. Pancreatic edema, hemorrhage and polymorphonuclear leukocyte infiltration decreased remarkably in the melatonin group compared to the control group. Conclusions: Injection of exogenous melatonin in rats with obstructive acute pancreatitis for seven days prevents acinar cell degeneration, apoptosis and polymorphonuclear leukocyte infiltration.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Arendt J. Melatonin and the pineal gland: influence on mammalian seasonal and circadian physiology. Rev Reprod 1998; 3: 13-22.
2. Gülben K, Ozdemir H, Berberoğlu U, et al. Melatonin modulates the severity of taurocholate-induced acute pancreatitis in the rat. Dig Dis Sci 2010; 55: 941-6.
3. Alhan E, Kalyoncu NI, Kural BV, Erçin C. Effects of melatonin on acute necrotizing pancreatitis in rats. Z Gastroenterol 2004; 42: 967-72.
4. Cöl C, Dinler K, Hasdemir AO, Bugdayci G. The effect of an intraperitoneal injection of melatonin on serum amylase levels in acute pancreatitis. JOP 2009; 10: 306-9.
5. Skandalakis LJ, Rowe JS, Gray SW, Skandalakis JE. Surgical embryology and anatomy of the pancreas. Surg Clin North Am 1993; 73: 661-97.
6. Sanfey H, Bulkley GB, Cameron JL. The pathogenesis of acute pancreatitis. The source and role of oxygen-derived free radicals in three different experimental models. Ann Surg 1985; 201: 633-9.
7. Banks PA, Freeman ML. Practice Parameters Committee of the American College of Gastroenterology. Practice guidelines in acute pancreatitis. Am J Gastroenterol 2006; 101: 2379-400.
8. Bulbuller N, Dogru O, Umac, et al. The effects of melatonin and pentoxiphylline on L-arginine induced acute pancreatitis. Ulus Travma Acil Cerrahi Derg 2005; 11: 108-14.
9. Kaiser AM, Saluja AK, Sengupta A, et al. Relationship between severity, necrosis, and apoptosis in five models of experimental acute pancreatitis. Am J Physiol 1995; 269: 1295-304.
10. Walker NI. Ultrastructure of the rat pancreas after experimental duct ligation. The role of apoptosis and intraepithelial macrophages in acinar cell deletion. Am J Pathol 1987; 126: 439-51.
11. Fujimoto K, Hosotani R, Wada M, et al. Ischemia-reperfusion injury on the pancreas in rats: identification of acinar cell apoptosis. J Surg Res 1997; 71: 127-36.
12. Muñoz-Casares FC, Padillo FJ, Briceño J, et al. Melatonin reduces apoptosis and necrosis induced by ischemia/reperfusion injury of the pancreas. J Pineal Res 2006; 40: 195203.
13. Ozturk F. Apoptosis. J Inonu University Medical Faculty 2002; 9: 2.
14. Carson DA, Ribeiro JM. Apoptosis and disease. Lancet 1993; 341: 1251-4.
15. Buja LM, Eigenbrodt ML, Eigenbrodt EH. Apoptosis and necrosis. Basic types and mechanisms of cell death. Arch Pathol Lab Med 1993; 117: 1208-14.
16. Jaworek J, Nawrot-Porabka K, Leja-Szpak A, et al. Melatonin as modulator of pancreatic enzyme secretion and pancreatoprotector. J Physiol Pharmacol 2007; 6: 65-80.
17. Konturek SJ, Konturek PC, Brzozowski, et al. Role of melatonin in upper gastrointestinal tract. J Physiol Pharmacol 2007; 6: 23-52.
18. Konturek SJ, Konturek PC, Brzozowska I, et al. Localization and biological activities of melatonin in intact and diseased gastrointestinal tract (GIT). J Physiol Pharmacol 2007; 58: 381-405.

APA	ÇÖL C, DİNLER K, HASDEMİR A, BÜYÜKAŞIK O, Fırat T, KUKNER A (2010). Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. , 433 - 438.
Chicago	ÇÖL Cavit,DİNLER Kahraman,HASDEMİR Ahmet Oğuz,BÜYÜKAŞIK Oktay,Fırat Tülin,KUKNER Aysel Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. (2010): 433 - 438.
MLA	ÇÖL Cavit,DİNLER Kahraman,HASDEMİR Ahmet Oğuz,BÜYÜKAŞIK Oktay,Fırat Tülin,KUKNER Aysel Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. , 2010, ss.433 - 438.
AMA	ÇÖL C,DİNLER K,HASDEMİR A,BÜYÜKAŞIK O,Fırat T,KUKNER A Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. . 2010; 433 - 438.
Vancouver	ÇÖL C,DİNLER K,HASDEMİR A,BÜYÜKAŞIK O,Fırat T,KUKNER A Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. . 2010; 433 - 438.
IEEE	ÇÖL C,DİNLER K,HASDEMİR A,BÜYÜKAŞIK O,Fırat T,KUKNER A "Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation." , ss.433 - 438, 2010.
ISNAD	ÇÖL, Cavit vd. "Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation". (2010), 433-438.

APA	ÇÖL C, DİNLER K, HASDEMİR A, BÜYÜKAŞIK O, Fırat T, KUKNER A (2010). Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. Turkish Journal of Gastroenterology, 21(4), 433 - 438.
Chicago	ÇÖL Cavit,DİNLER Kahraman,HASDEMİR Ahmet Oğuz,BÜYÜKAŞIK Oktay,Fırat Tülin,KUKNER Aysel Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. Turkish Journal of Gastroenterology 21, no.4 (2010): 433 - 438.
MLA	ÇÖL Cavit,DİNLER Kahraman,HASDEMİR Ahmet Oğuz,BÜYÜKAŞIK Oktay,Fırat Tülin,KUKNER Aysel Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. Turkish Journal of Gastroenterology, vol.21, no.4, 2010, ss.433 - 438.
AMA	ÇÖL C,DİNLER K,HASDEMİR A,BÜYÜKAŞIK O,Fırat T,KUKNER A Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. Turkish Journal of Gastroenterology. 2010; 21(4): 433 - 438.
Vancouver	ÇÖL C,DİNLER K,HASDEMİR A,BÜYÜKAŞIK O,Fırat T,KUKNER A Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation. Turkish Journal of Gastroenterology. 2010; 21(4): 433 - 438.
IEEE	ÇÖL C,DİNLER K,HASDEMİR A,BÜYÜKAŞIK O,Fırat T,KUKNER A "Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation." Turkish Journal of Gastroenterology, 21, ss.433 - 438, 2010.
ISNAD	ÇÖL, Cavit vd. "Evaluation of the effects of melatonin administration intraperitoneally on rats with acute pancreatitis induced by ductal ligation". Turkish Journal of Gastroenterology 21/4 (2010), 433-438.