Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase

GÜNGÖRMEDİ, Gökhan; BENLİ, Eda; ÇABUK, AHMET; Aytar Çelik, Pınar; GEDIKLI, Serap

Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase

Eda BENLİ, (Eskişehir Osmangazi Üniversitesi, Eskişehir, Türkiye)

Serap GEDIKLI, (Eskişehir Osmangazi Üniversitesi, Eskişehir, Türkiye)

PINAR AYTAR ÇELİK, (Eskişehir Osmangazi Üniversitesi, Eskişehir, Türkiye)

Gökhan GÜNGÖRMEDİ, (Eskişehir Osmangazi Üniversitesi, Eskişehir, Türkiye)

Ahmet ÇABUK (Eskişehir Osmangazi Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Eskişehir, Türkiye)

IUFS Journal of Biology

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Yıl: 2011 Cilt: 70 Sayı: 2 Sayfa Aralığı: 25 - 34 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase

Öz:

2,4-diklorofenol pestisitlerin üretiminde kullanılan rekalsitrant bir bileşiktir. Buğday kepeği ile indüklenmiş patates dekstroz broth besiyerinde üretilen Trametes versicolor ATCC (20080l)'den elde edilen ham lakkaz ile 2,4-diklorofenolden klorun uzaklaştırılması değerlendirilmiştir. pH, substrat konsantrasyonu, reaksiyon süresi, sıcaklığı ve enzim miktarı gibi deklorinasyon için optimizasyon parametreleri çalışılmıştır. Bu çalışmaların sonunda pH 4, 400 p,M başlangıç substrat konsantrasyonu, reaksiyon süresi 7 dakika, sıcaklık 30 °C seçilmiştir. Daha once belirlenen optimum şartlarda oksijen tüketimi ile deklorinasyon süreci arasındaki ilişki incelenmiştir v.e çözünmüş oksijende azalma gözlemlenmiştir. Bu bileşiğin deklorinasyonundan sonra kimyasal yapısındaki değişiklikler FT-IR analizi ile, toksisitesindeki değişimler ise Microtox ile belirlenmiştir. Spektradaki yayvan ve şiddetli pikler; yük transferi ve elektrostatik etkileşimlerle meydana gelen deklorinasyona bir kanıt olarak gösterilebilir. Toksisite deneyleri, 2,4-DCP'nin deklorinasyona ilaveten detoksifıye olduğunu da göstermiştir. Bunun yanısıra deklorinasyon deneylerinde istatistik analizler gerçekleştirilmiştir.

Anahtar Kelime: toksik maddeler Coriolus versicolor zararlı öldürücüler detoksifikasyon enzimatik yöntemler 2,4-D toksisite lakkaz deklorizasyon

Konular: Biyoloji

İndüklenmiş lakkaz ile 2,4-diklorofenolün dehalojenasyonu ve detoksifikasyonu

Öz:

2,4-dichlorophenol is a recalcitrant compound which is used in the production of pesticides. Chlorine removal of 2,4-dichlorophenol with the crude laccase produced Trametes versicolor ATCC (200801) in potato dextrose broth including wheat bran was investigated. The optimization parameters for dechlorination such as pH, initial substrate concentration, reaction period, reaction temperature and amount of enzyme were examined. At the end of these studies, pH 4, 400 p,M of initial substrate concentration, 7 min of reaction time, 30 °C of temperature were selected. It was tested that correlation with oxygen consumption and dechlorination processes under the determined optimum conditions before and the decrease of dissolved oxygen was observed. Also, after the dechlorination of this compound, changes in chemical structure of the compound were determined with FTIR analysis and toxicity alterations with Microtox test were studied. Wide and intense peaks at spectra may be an evidence to dechlorination occurring with load transfers and electrostatic interactions. Toxicity experiments showed that 2,4-DCP was detoxified in addition to dechlorination. Besides, statistical analyses were performed for dechlorination experiments.

Anahtar Kelime: 2,4-D toxicity laccase dechlorination toxic substances Coriolus versicolor pesticides detoxification enzymatic methods

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	BENLİ E, GEDIKLI S, Aytar Çelik P, GÜNGÖRMEDİ G, ÇABUK A (2011). Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. , 25 - 34.
Chicago	BENLİ Eda,GEDIKLI Serap,Aytar Çelik Pınar,GÜNGÖRMEDİ Gökhan,ÇABUK AHMET Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. (2011): 25 - 34.
MLA	BENLİ Eda,GEDIKLI Serap,Aytar Çelik Pınar,GÜNGÖRMEDİ Gökhan,ÇABUK AHMET Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. , 2011, ss.25 - 34.
AMA	BENLİ E,GEDIKLI S,Aytar Çelik P,GÜNGÖRMEDİ G,ÇABUK A Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. . 2011; 25 - 34.
Vancouver	BENLİ E,GEDIKLI S,Aytar Çelik P,GÜNGÖRMEDİ G,ÇABUK A Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. . 2011; 25 - 34.
IEEE	BENLİ E,GEDIKLI S,Aytar Çelik P,GÜNGÖRMEDİ G,ÇABUK A "Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase." , ss.25 - 34, 2011.
ISNAD	BENLİ, Eda vd. "Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase". (2011), 25-34.

APA	BENLİ E, GEDIKLI S, Aytar Çelik P, GÜNGÖRMEDİ G, ÇABUK A (2011). Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. IUFS Journal of Biology, 70(2), 25 - 34.
Chicago	BENLİ Eda,GEDIKLI Serap,Aytar Çelik Pınar,GÜNGÖRMEDİ Gökhan,ÇABUK AHMET Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. IUFS Journal of Biology 70, no.2 (2011): 25 - 34.
MLA	BENLİ Eda,GEDIKLI Serap,Aytar Çelik Pınar,GÜNGÖRMEDİ Gökhan,ÇABUK AHMET Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. IUFS Journal of Biology, vol.70, no.2, 2011, ss.25 - 34.
AMA	BENLİ E,GEDIKLI S,Aytar Çelik P,GÜNGÖRMEDİ G,ÇABUK A Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. IUFS Journal of Biology. 2011; 70(2): 25 - 34.
Vancouver	BENLİ E,GEDIKLI S,Aytar Çelik P,GÜNGÖRMEDİ G,ÇABUK A Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase. IUFS Journal of Biology. 2011; 70(2): 25 - 34.
IEEE	BENLİ E,GEDIKLI S,Aytar Çelik P,GÜNGÖRMEDİ G,ÇABUK A "Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase." IUFS Journal of Biology, 70, ss.25 - 34, 2011.
ISNAD	BENLİ, Eda vd. "Dehalogenation and detoxification of 2,4-dichlorophenol with induced laccase". IUFS Journal of Biology 70/2 (2011), 25-34.