The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments

BADAK, M. İsmail; DISCIGIL, BERENT; GÜRCÜN, Uğur; BOĞA, Mehmet; ÖZKISACIK, Erdem Ali; KURTOĞLU, Tünay; DURMAZ, Selim

The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments

Erdem Ali ÖZKISACIK, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

Berent DİŞÇİGİL, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

Tünay KURTOĞLU, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

Selim DURMAZ, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

Uğur GÜRCÜN, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

Mehmet BOĞA, (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

M. İsmail BADAK (Adnan Menderes Üniversitesi, Tıp Fakültesi, Kalp ve Damar Cerrahisi Anabilim Dalı, Aydın, Türkiye)

Türk Göğüs Kalp Damar Cerrahisi Dergisi

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Yıl: 2012 Cilt: 20 Sayı: 3 Sayfa Aralığı: 584 - 589 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments

Öz:

Amaç: Bu çalışmada, asetilsalisilik asit (ASA), klopidogrel ve tirofiban gibi antitrombositer ajanların sıçan torasik aort segmentinde asetilkolin (ACh) ile uyarılan gevşeme yanıtları üzerine olan etkileri araştırıldı. Çalışma planı: Yirmi sekiz adet ağırlıkları 250-300 g arasında olan Wistar-albino türü yetişkin erkek sıçan çalışmaya alındı. Sıçanlar randomize olarak dört gruba ayrıldı: ASA grubu (n=7), klopidogrel grubu (n=7), tirofiban grubu (n=7) ve kontrol grubu (n=7). Deneyden 12 saat önce intraperitoneal (İP) ASA (1.5 mg/kg) veya klopidogrel (1 mg/kg) enjeksiyonları yapıldı. Tirofiban grubunda, 150 μgr/kg tirofiban İP deneyden 1 ve 12 saat önce olmak üzere, iki kez verildi. Kontrol grubundaki sıçanlara herhangi bir ilaç verilmedi. Sıçanlar dekapite edildikten sonra torasik aort segmentleri çıkartıldı ve oksijenize Krebs çözeltisi içinde organ banyosuna asıldı ve aort halkalarında asetilkolin ile uyarılan gevşeme yanıtının doza bağımlı değişimleri incelendi. Bulgular: Asetilkolinin en düşük molar konsantrasyonu (10-9) ile elde edilen gevşeme yanıtının ASA (%94.3±3.3) ve klopidogrel (%94.6±3.1) grubunda kontrol (%86.7±8.1) grubuna göre azalmış olduğu bulundu (p<0.05). Daha yüksek asetilkolin konsantrasyonlarında ise, tüm gruplarda gevşeme yanıtlarının benzer olduğu gözlemlendi. Sonuç: Asetilsalisilik asit ve klopidogrel tedavilerinin sonucunda endotel aracılı gevşeme yanıtında hafif bir gecikme ortaya çıkmış ve tirofibanın belirgin bir etkisi olmamıştır. Bu bulgular ASA ve klopidogrelin endotel aracılı vazodilatasyon üzerinde sınırlı bir etkisi olabileceğini düşündürmektedir.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi Cerrahi

Klopidogrel, asetilsalisilik asit ve tirofibanın sıçan torasik aort segmentlerinde asetilkolin ile uyarılan gevşeme üzerine etkisi

Öz:

Background: In this article, we aimed to investigate the effects of antithrombotic agents, including acetyl salicylic acid (ASA), clopidogrel, and tirofiban on the acetylcholine (ACh)- induced dilation responses in rat thoracic aorta segments. Methods: Twenty-eight Wistar albino male rats weighing between 250-300 g were included in the study. The rats were randomly divided into four groups: the ASA group (n=7), the clopidogrel group (n=7), the tirofiban group (n=7), and the control group (n=7). Intraperitoneal (IP) injection of ASA (1.5 mg/kg) or clopidogrel (1 mg/kg) was administered 12 hours before the experiment. In the tirofiban group, 150 μgr/kg tirofiban IP was given twice at one and 12 hours before the experiment. The rats in the control group did not receive any medication. After the rats were decapitated, the segments of the thoracic aorta were removed and suspended in an oxygenated Krebs solution in a tissue bath, and the dose dependency in the responses to the ACh-induced dilation in the aortic rings were studied. Results: The dilation responses of the aortic rings to ACh in the lowest molar concentration (10-9) were found to be reduced in the ASA (94.3%±3.3) and clopidogrel (94.6%±3.1) groups when compared with the control group (86.7%±8.1) (p<0.05). The responses to higher concentrations of ACh were observed to be similar in all of the groups. Conclusion: Acetyl salicylic acid and clopidogrel treatments resulted in a slight delay of endotheliummediated relaxation while tirofiban had no significant effect. These findings suggest that ASA and clopidogrel may have a limited effect on endothelium-mediated vasodilation.

Anahtar Kelime:

Konular: Kalp ve Kalp Damar Sistemi Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	ÖZKISACIK E, DISCIGIL B, KURTOĞLU T, DURMAZ S, GÜRCÜN U, BOĞA M, BADAK M (2012). The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. , 584 - 589.
Chicago	ÖZKISACIK Erdem Ali,DISCIGIL BERENT,KURTOĞLU Tünay,DURMAZ Selim,GÜRCÜN Uğur,BOĞA Mehmet,BADAK M. İsmail The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. (2012): 584 - 589.
MLA	ÖZKISACIK Erdem Ali,DISCIGIL BERENT,KURTOĞLU Tünay,DURMAZ Selim,GÜRCÜN Uğur,BOĞA Mehmet,BADAK M. İsmail The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. , 2012, ss.584 - 589.
AMA	ÖZKISACIK E,DISCIGIL B,KURTOĞLU T,DURMAZ S,GÜRCÜN U,BOĞA M,BADAK M The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. . 2012; 584 - 589.
Vancouver	ÖZKISACIK E,DISCIGIL B,KURTOĞLU T,DURMAZ S,GÜRCÜN U,BOĞA M,BADAK M The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. . 2012; 584 - 589.
IEEE	ÖZKISACIK E,DISCIGIL B,KURTOĞLU T,DURMAZ S,GÜRCÜN U,BOĞA M,BADAK M "The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments." , ss.584 - 589, 2012.
ISNAD	ÖZKISACIK, Erdem Ali vd. "The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments". (2012), 584-589.

APA	ÖZKISACIK E, DISCIGIL B, KURTOĞLU T, DURMAZ S, GÜRCÜN U, BOĞA M, BADAK M (2012). The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. Türk Göğüs Kalp Damar Cerrahisi Dergisi, 20(3), 584 - 589.
Chicago	ÖZKISACIK Erdem Ali,DISCIGIL BERENT,KURTOĞLU Tünay,DURMAZ Selim,GÜRCÜN Uğur,BOĞA Mehmet,BADAK M. İsmail The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. Türk Göğüs Kalp Damar Cerrahisi Dergisi 20, no.3 (2012): 584 - 589.
MLA	ÖZKISACIK Erdem Ali,DISCIGIL BERENT,KURTOĞLU Tünay,DURMAZ Selim,GÜRCÜN Uğur,BOĞA Mehmet,BADAK M. İsmail The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. Türk Göğüs Kalp Damar Cerrahisi Dergisi, vol.20, no.3, 2012, ss.584 - 589.
AMA	ÖZKISACIK E,DISCIGIL B,KURTOĞLU T,DURMAZ S,GÜRCÜN U,BOĞA M,BADAK M The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2012; 20(3): 584 - 589.
Vancouver	ÖZKISACIK E,DISCIGIL B,KURTOĞLU T,DURMAZ S,GÜRCÜN U,BOĞA M,BADAK M The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments. Türk Göğüs Kalp Damar Cerrahisi Dergisi. 2012; 20(3): 584 - 589.
IEEE	ÖZKISACIK E,DISCIGIL B,KURTOĞLU T,DURMAZ S,GÜRCÜN U,BOĞA M,BADAK M "The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments." Türk Göğüs Kalp Damar Cerrahisi Dergisi, 20, ss.584 - 589, 2012.
ISNAD	ÖZKISACIK, Erdem Ali vd. "The effects of clopidogrel, acetyl salicylic acid and tirofiban on acetylcholine-induced dilation in rat thoracic aorta segments". Türk Göğüs Kalp Damar Cerrahisi Dergisi 20/3 (2012), 584-589.