Yıl: 2012 Cilt: 36 Sayı: 3 Sayfa Aralığı: 255 - 265 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey

Öz:
Beauveria bassiana (Balsamo) Vuillemin ve Metarhizium anisopliae var. anisopliae (Metschnikoff ) Sorokin dünyadaki mikrobiyal kontrol programlarında kullanılan en yaygın entomopatojenik funguslardır. Bu 2 önemli türün genetik çeşitliliğinin değerlendirilmesi etkili biyolojik mücadele stratejilerinin geliştirilmesi ve yapay epizootiklerin etkisinin değerlendirilmesinde faydalıdır. Bu çalışmada, Türkiye’nin Doğu Karadeniz Bölgesi’nden elde edilen 13 adet B. bassiana ve 33 adet M. anisopliae var. anisopliae suşu içerisindeki genetik çeşitlilik Çoğaltılmış Parça Uzunluk Polimorfizm (ÇPUP) tekniği ve izoenzim analizi kullanarak belirlenmiştir. ÇPUP verilerinin küme analizi hem B. bassiana hem de M. anisopliae var. anisopliae suşlarını sırasıyla 3 ve 4 farklı gruba ayırmıştır. α ve β- esteraz band paterni M. anisopliae var. anisopliae izolatlarını açıkça ayırmasına rağmen, B. bassiana izolatları için yeterli bilgi vermemiştir. Ayrıca, bu çalışmada bütün izolatların farklı sıcaklıklarda (8, 16, 25, 37 °C), UV muamelesinde (30 ve 60 dak.) ve Tenebrio molitor’a karşı virulansı belirlenmiştir. Bu sonuçlar, bu bölgedeki B. bassiana ve M. anisopliae var. anisopliae populasyonlarında önemli bir genetik çeşitliliğin olduğunu göstermektedir. B. bassiana suşlarının çeşitliliği coğrafik konumla ilişkili görülmesine rağmen habitat tipi ile ilişkili değildir. B. bassiana izolatları arasında farklı sıcaklıklarda, UV muamelesinde ve patojenite bakımından da bir ilişki tespit edilmemiştir. M. anisopliae var. anisopliae suşlarının çeşitliliği ise ne coğrafik konumla ne de habitat tipi ile ilişkilidir. M. anisopliae var. anisopliae izolatları arasında da farklı sıcaklıklarda (16 °C hariç), UV muamelesinde ve patojenite bakımından bir ilişki tespit edilmemiştir. Burada verilen bilgiler bu bölgedeki fındık zararlıları ile mücadelede faydalı olabilir.
Anahtar Kelime: Metarhizium anisopliae AFLP entomopatojen mantarlar genotip çevre etkileşimi biyolojik kontrol ajanları lokalizasyon sıcaklık Beauveria bassiana Türkiye bitki zararlıları Karadeniz Bölgesi genetik çeşitlilik Deuteromycotina

Konular: Biyoloji

Türkiye’nin Doğu Karadeniz Bölgesi’nden elde edilen Metarhizium anisopliae var. anisopliae ve Beauveria bassiana izolatlarının genetik çeşitliliği

Öz:
Beauveria bassiana (Balsamo) Vuillemin and Metarhizium anisopliae var. anisopliae (Metschnikoff ) Sorokin are the most common entomopathogenic fungi used in microbial control programs all over the world. Assessments of the genetic variability of these 2 important species are useful for the development of effective biocontrol strategies and for evaluating the impact of artificial epizootics. In this study, the genetic diversity of 13 B. bassiana and 33 M. anisopliae var. anisopliae strains isolated from the hazelnut-growing region of Turkey was determined by using the amplifi ed fragment length polymorphism (AFLP) and α- and β-isoenzyme analyses. Cluster analysis of AFLP data clearly separated both B. bassiana and M. anisopliae var. anisopliae strains into 3 and 4 different groups, respectively. While α- and β-esterase banding patterns clearly separated M. anisopliae var. anisopliae strains, it did not give enough information about B. bassiana strains. We also assessed the growing ability of all isolates at different temperatures (8, 16, 25, and 37 °C) and UV exposures (30 and 60 min), and virulence against Tenebrio molitor. These results indicated that there is a significant variability within the B. bassiana and M. anisopliae var. anisopliae populations in this region. Although the diversity of B. bassiana isolates is associated with geographic location, it is not associated with habitat type. There is also no association amongst B. bassiana isolates in terms of the ability to grow at different temperatures and UV exposures, or virulence against T. molitor. The diversity of M. anisopliae var. anisopliae strains is neither associated with habitat type nor geographic location, and there is no association amongst M. anisopliae var. anisopliae strains in terms of the ability to grow at different temperatures (except for 16 °C) and UV exposure, or virulence against T. molitor. The data presented here might be useful for controlling some hazelnut pests in this region.
Anahtar Kelime: Beauveria bassiana Turkey plant pests Black Sea Region genetic variation Deuteromycotina Metarhizium anisopliae AFLP entomogenous fungi genotype environment interaction biological control agents localization temperature

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • 1. Zimmermann G. Pflanzenschutz-Nachr. Bayer 45: 113-128, 1992.
  • 2. Zimmermann G. Review on safety of the entomopathogenic fungi Beauveria bassiana and Beauveria brongniartii. Biocont Sci Technol 17: 553-596, 2007.
  • 3. Zimmermann G. Review on safety of the entomopathogenic fungus Metarhizium anisopliae. Biocont Sci Technol 17: 879- 920, 2007.
  • 4. Meyling NV, Eilenberg J. Ecology of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in temperate agroecosystems: potential for conservation biological control. Biol Cont 43: 145-155, 2007.
  • 5. Tuncer C, Ecevit O. Current status of hazelnut pests in Turkey. In: Köksal AI, Okay Y, Güsnes NT. eds. IV International Symposium on Hazelnut. ISHS; 1997: pp. 545-552.
  • 6. Sevim A, Demir I, Höfte M et al. Isolation and characterization of entomopathogenic fungi from hazelnut-growing region of Turkey. Bio Control 55: 279-297, 2010.
  • 7. Mueller UG, Wolfenbarger LL. AFLP genotyping and fingerprinting. Trends Ecol Evol 14: 389-394, 1999.
  • 8. Bridge PD, Abraham YJ, Cornish MC et al. The chemotaxonomy of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolates from the coffee berry borer Hypothenemus hampei (Coleoptera: Scolytidae). Mycopathologia 111: 85-90, 1990.
  • 9. St Leger RJ, Allee LL, May B et al. World-wide distribution of genetic variation in Beauveria spp. Mycol Res 96: 1007-1015, 1992.
  • 10. Louela AC, Brooks WM. Differentiation of Beauveria bassiana isolates from the darkling beetle, Alphitobius diaperinus, using isozyme and RAPD analyses. J Invertebr Pathol 72: 190-196, 1998.
  • 11. Riba G, Bouvier-Fourcade I, Caudal A. Isoenzyme polymorphism in Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) entomogenous fungi. Mycopathologia 96: 161-169, 1986.
  • 12. St Leger RJ, May B, Allee LL et al. Genetic diff erences in allozymes and in formation of infection structures among isolates of the entomopathogenic fungus Metarhizium anisopliae. J Invertebr Pathol 60: 89-101, 1992.
  • 13. Fernandes EKK, Costa GL, Moraes AML et al. Study on morphology, pathogenicity, and genetic variability of Beauveria bassiana isolates obtained from Boophilus microplus tick. Parasitol Res 98: 324-332, 2006.
  • 14. Cruz LP, Gaitan AL, Gongora CE. Exploiting the genetic diversity of Beauveria bassiana for improving the biological control of the coff ee berry borer through the use of strain mixtures. Appl Microbiol Biotechnol 71: 918-926, 2006.
  • 15. Muro MA, Mehta S, Moore D. The use of amplifi ed fragment length polymorphism for molecular analysis of Beauveria bassiana isolates from Kenya and other countries, and their correlation with host and geographical origin. FEMS Microbiol Lett 229: 249-257, 2003.
  • 16. Aquino de Muro M, Elliott S, Moore D et al. Molecular characterisation of Beauveria bassiana isolates obtained from overwintering sites of Sunn Pests (Eurygaster and Aelia species). Mycol Res 109: 294-306, 2005.
  • 17. Maurer P, Couteaudier Y, Girard PA et al. Genetic diversity of Beauveria bassiana and relatedness to host insect range. Mycol Res 10: 159-164, 1997.
  • 18. Bidochka MJ, Kamp AM, Lavender TM et al. Habitat association in two genetic groups of the insect-pathogenic fungus Metarhizium anisopliae: Uncovering cryptic species? Appl Environ Microbiol 67: 1335-1342, 2001.
  • 19. Inglis GD, Duke GM, Goettel MS et al. Genetic diversity of Metarhizium anisopliae var. anisopliae in southwestern British Columbia. J Invertebr Pathol 98: 101-113, 2008.
  • 20. Velásquez VB, Cárcamo MP, Meriño CR et al. Intraspecific differentiation of Chilean isolates of the entomopathogenic fungi Metarhizium anisopliae var. anisopliae as revealed by RAPD, SSR and ITS markers. Gen Mol Biol 30: 89-99, 2007.
  • 21. Fungaro MHP, Vieira MLC, Pizzirani-Kleiner AA et al. Diversity among soil and insect isolates of Metarhizium anisopliae var. anisopliae detected by RAPD. Lett Appl Microbiol 22: 389-392, 1996.
  • 22. The Ministry of Agriculture of Turkey. East Black Sea Regional Agriculture Infi nitive Plan. Ministry of Agriculture. Ankara; 2007.
  • 23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal Biochem 72: 248-254, 1976.
  • 24. Vos P, Hogers R, Bleeker M et al. AFLP: new technique for DNA fingerprinting. Nucl Acids Res 23: 4407-4414, 1995.
  • 25. Schlüter PM, Harris SA. Analysis of multilocus fi ngerprinting data sets containing missing data. Mol Ecol Notes 6: 569-572, 2006.
  • 26. Van de Peer Y, De Wachter R. TREECON for Windows: a soft ware package for the construction and drawing of evolutionary trees for the Microsoft Windows environment. Comput Appl Biosci 10: 569-570, 1994.
  • 27. Jaccard P. Nouvelles recherches sur la distribution florale. Bull Vaud Soc Nat Sci 44: 223-270, 1908.
  • 28. Nei M, Li WH. Mathematical model for studying genetic variation in term of restriction endonucleases. Proc Natl Acad Sci USA 76: 5269-5273, 1979.
  • 29. Sokal RR, Michener CD. A statistical method for evaluating systematic relationships. Univ Kansas Sci Bull 38: 1409-1438, 1958.
  • 30. Abbott WS. A method of computing the eff ectiveness of an insecticide. J Econ Entomol 18: 265-267, 1925.
  • 31. Th akur R, Rajak RC, Sandhu SS. Biochemical and molecular characteristics of indigenous strains of the entomopathogenic fungus Beauveria bassiana of Central India. Biocont Sci Technol 15: 733-744, 2005.
  • 32. Bidochka MJ, Menzies FV, Kamp AM. Genetic groups of the insect-pathogenic fungus Beauveria bassiana are associated with habitat and thermal growth preferences. Arch Microbiol 178: 531-537, 2002.
  • 33. Enkerli J, Kölliker R, Keller S et al. Isolation and characterization of microsatellite markers from the entomopathogenic fungus Metarhizium anisopliae. Mol Ecol Notes 5: 384-386, 2005.
  • 34. Fegan M, Manners JM, Maclean DJ et al. Random amplified polymorphic DNA markers reveal a high degree of genetic diversity in the entomopathogenic fungus Metarhizium anisopliae var. anisopliae. J Gen Microbiol 139: 2075-2081, 1993.
  • 35. Takatsuka J. Characterization of Beauveria bassiana isolates from Japan using inter-simple-sequence-repeat-anchored polymerase chain reaction (ISSR-PCR) amplifi cation. Appl Entomol Zool 42: 563-571, 2007.
  • 36. Urtz BE, Rice WC. RAPD-PCR characterization of Beauveria bassiana isolates from the rice water weevil Lissorhoptrus oryzophilus. Lett Appl Microbiol 25: 405-409, 1997.
  • 37. Poprawski TJ, Riba G, Jones WA et al. Variation in isoesterase of geographical population of Beauveria bassiana (Deuteromycotina: Hyphomycetes) isolated from Sitona weevils (Coleoptera: Curculionidae). Environ Entomol 17: 275-279, 1988.
APA SEVIM A, HÖFTE M, DEMİRBAĞ Z (2012). Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. , 255 - 265.
Chicago SEVIM ALI,HÖFTE Monica,DEMİRBAĞ ZİHNİ Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. (2012): 255 - 265.
MLA SEVIM ALI,HÖFTE Monica,DEMİRBAĞ ZİHNİ Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. , 2012, ss.255 - 265.
AMA SEVIM A,HÖFTE M,DEMİRBAĞ Z Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. . 2012; 255 - 265.
Vancouver SEVIM A,HÖFTE M,DEMİRBAĞ Z Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. . 2012; 255 - 265.
IEEE SEVIM A,HÖFTE M,DEMİRBAĞ Z "Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey." , ss.255 - 265, 2012.
ISNAD SEVIM, ALI vd. "Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey". (2012), 255-265.
APA SEVIM A, HÖFTE M, DEMİRBAĞ Z (2012). Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. Turkish Journal of Biology, 36(3), 255 - 265.
Chicago SEVIM ALI,HÖFTE Monica,DEMİRBAĞ ZİHNİ Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. Turkish Journal of Biology 36, no.3 (2012): 255 - 265.
MLA SEVIM ALI,HÖFTE Monica,DEMİRBAĞ ZİHNİ Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. Turkish Journal of Biology, vol.36, no.3, 2012, ss.255 - 265.
AMA SEVIM A,HÖFTE M,DEMİRBAĞ Z Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. Turkish Journal of Biology. 2012; 36(3): 255 - 265.
Vancouver SEVIM A,HÖFTE M,DEMİRBAĞ Z Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey. Turkish Journal of Biology. 2012; 36(3): 255 - 265.
IEEE SEVIM A,HÖFTE M,DEMİRBAĞ Z "Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey." Turkish Journal of Biology, 36, ss.255 - 265, 2012.
ISNAD SEVIM, ALI vd. "Genetic variability of Beauveria bassiana and Metarhizium anisopliae var. anisopliae isolates obtained from the Eastern Black Sea Region of Turkey". Turkish Journal of Biology 36/3 (2012), 255-265.