Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri
Yıl: 2012 Cilt: 21 Sayı: 83 Sayfa Aralığı: 61 - 67 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022
Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri
Öz: Sunulan çalışmada parazitoid Pimpla turionellae (Hymenoptera:Ichneumonidae) de kadmiyumun protein ve glikojen sentezine olan etkileri araştırılmıştır. Böceğin sentezlediği total protein miktarı 20. günden itibaren besindeki kadmiyumdan önemli ölçüde etkilenmiştir. 20. günde tüm kadmiyum konsantrasyonlarında protein miktarı kontrole göre azalırken, 30. günde en düşük kadmiyum konsantrasyonunda artmış, en yüksek konsantrasyonda ise önemli ölçüde azalmıştır. Böceğin sentezlediği glikojen miktarı ise 10. günden itibaren kadmiyumdan etkilenmiş ve kontrole göre artışlar göstermiştir. 20. günde 0,10 ve 0,50 mg kadmiyum içeren besinle beslenen böceklerde glikojen miktarı önemli ölçüde artarken, bu artış 30. günde yalnız yüksek kadmiyum konsantrasyonlarında (0,50 ve 1 mg Cd) gözlenmiştir. P. turionellae’ da ağır metal stresi altında gözlenen bu değişimler ağır metallerin biyolojik kontrol ajanı olarak kullanılabilen faydalı türler üzerine olan etkilerini ve P. turionellae’ nin ağır metal kirliliğinin belirlenmesinde bir biyoindikatör olarak kullanılabilineceğini göstermesi bakımından önemlidir.
Anahtar Kelime: Konular:
Effects of heavy metal stress on protein and glycogen synthesis of Pimpla turionellae (Hymenoptera:Ichneumonidae)
Öz: In the present study, effects of cadmium on protein and glycogen synthesis of parasitoid Pimpla turionellae (Hymenoptera:Ichneumonidae) were investigated. The amount of total protein synthesized by insect significantly affected by cadmium from the day of 20th. Protein amount decreased in all concentrations of Cd compared to control at this day. 30th day, the amount of protein in insects fed on diet with the lowest Cd concentration increased, while the highest concentration decreased significantly. The amount of glycogen synthesized by insect affected by Cd from the day of 10th, and showed increases compare to control. Glycogen amount of insects fed on diet containing 0.10 and 0.50 mg Cd significantly increased on day 20th. This increase was observed in only high concentrations of cadmium (0.50 and 1 mg Cd) on day 30th. These changes observed P. turionellae under the heavy metal stress are more significant for observing the effects of heavy metals on beneficial species use as biologic control agents and be able to use for bioindicator to heavy metal contamination.
Anahtar Kelime: Konular:
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
- Bischof C (1996) Effects of heavy metal stress on free amino acids in the haemolymph and total body tissue of Lymantria dispar larvae parasitized by Glyptapanteles liparidis Entomologia experimentalis et applicata. 79 (1): 61-68.
- Bischof C (1995) Effects of heavy metal stress on carbohydrate and lipid concentrations in the haemolymph and total body tissue of parasitized Lymantria Dispar L. Larvae (Lepidoptera) Comparative Biochemistry and Physiology 112 (C1): 87-92.
- Braeckman B, Smagghe G, Brutsaert N, Cornelis R, Raes H (1999) Cadmium uptake and defense mechanism in insect cells. Environmental Research Section A 80: 231-243.
- Bream AS (2003) Laboratory evaluation of heavy metals stress on certain biochemical parameters of the aquatic insect, Sphaerodema urinator Duf. (Hemiptera: Belostomatidae). Communications in Agricultural and Applied Biologicl Science 68 (4 Pt A): 291-297.
- Brown C, Nestler C (1985) Catecholamines and Indolalkylamines. In: GA Kerkut and LI Gilbert (eds). Comparative Insect Physiology, Biochemistry and Pharmacology, Pergamon Press, New York, 435-497.
- Burmester T (2001) Molecular evolution of the arthropod hemocyanin superfamily. Molecular Biology and Evolution 18 (2): 184-195.
- Carr RS, Williams JW, Saska FI, Buhl RS, Neff JM (1985) Bioenergetic alterations correlated with growth, fecundity and body burdens of cadmium in Mysids (Mysidopsis bahia). Environmental Toxicolgy and Chemistry 4: 181-188.
- Cervera A, Maymo AC, Martinez-Pardo R, Garcera, MD (2006) Vitellogenin polypeptide levels in one susceptible and one cadmium-resistant strain of Oncopeltus fasciatus (Heteroptera: Lygaeidae) and its role in cadmium resistance. Journal of Insect Physiology 52: 158-168.
- Cervera A, Maymo AC, Martinez-Pardo R, Garcera MD (2004) Cadmium effects on development and reproduction of Oncopeltus fasciatus (Heteroptera: Lygaeidae). Journal of Insect Physiology 50: 737-749.
- Chen P (1985) Amino acid and protein metabolism. In: Kerkut GA, Gilbert LI (eds), Compherensive Insect Physiology, Biochemistry and Pharmacology, Pergamon Press, Oxford, 177-199.
- Chinni S, Yallapragada PR (2002) Energy levels of Panaeus indicus postlarvae on exposure to lead. Ecotoxicology and Environmental Safety 52: 173-179.
- Chrestensen CA, Starke DW, Mieyal JJ (2000) Acute cadmium exposure inactivates thioltransferase (glutaredoxin), inhibits intracellular reduction of protein-glutathionyl-mixed disulfides, andinitiates apoptosis. Journal of Biological Chemistry 275: 26556–26565.
- Dadd RH (1985) Nutrition: Organism. In: Kerkut GA, Gilbert LI (eds), Compherensive Insect Physiology, Biochemistry and Pharmacology, Pergamon Press, Oxford, 313–390.
- Devkota B, Schmidt GH (1999) Effect of mercury (Hg2+) on thelife span and development of Eyprepocnemis plorans (Charp.) (Orthopteroidea, Caelifera, Acrididae). Bollettino del Laboratorio di Entomologia Agraria ‘‘Filippo Silvestri’’ 55: 113-125.
- Devkota B, Schmidt GH (2000) Accumulation of heavy metals in food plants and grassopher from the Taigetos Mountains, Greece. Agriculture Ecosystems and Environment 78: 85-91.
- Emre İ (1988) Meridik bir besinin Pimpla turionellae L. (Hymenoptera: Ichneumonidae) ergin dişilerinin yumurta verimine etkisi. Doğa Türk Biyoloji Dergisi 12 (2): 101-105.
- Fisher R (1987) Ecological studies on the pupal parasites (Hym., Ichneumonidae) of four native species of Yponomeuta (Lep., Yponomeutidae). Journal of Applied Entomology 103: 515-523.
- Florea AM, Büsselberg D (2006) Occurrence, use and potential toxic effects of metals and metal compounds. Biometals 19: 419-427.
- Fountain MT, Hopkin SP (2001) Continuous monitoring of Folsomia candida (Insecta: Collembola) in a metal exposure test. Ecotoxicology and Environmental Safety 48: 275–286.
- Gregory JL (1997) The effect of cadmium on cytosolic free calcium, protein kinase and collagen synthesis in rat osteosarchoma (Ros17/2.8) Cells. Toxicology and Applied Pharmacology 143: 189-195.
- Hsu MJ, Selvaraj K, Agoramoorthy G (2006) Taiwan’s industrial heavy metal pollution threatens terrestrial biota. Environmental Pollution 143: 327-334.
- Ilijin L, Peric-Mataruga V, Radojicic R, Lazarevic J, Nenadovic V, Vlahovic M, Mrdakovic M (2010) Effect of cadmium on protocerebral neurosecretory neurons and fitness components in Lymantria dispar L. Folia Biologica (Krakow) 58: 91-99.
- Islam A, Roy S (1983) Effects of CdCl2 on the quantitative various of Carbohydrate, protein, aminoacid and cholesterol in Chrysochoris stolli Wolf (Insecta: Hemiptera). Current Science 52: 215-217.
- Ivanovic J, Jankovic-Hladni M (1991) Hormones and Methabolisms in Insect Stress. CRC Press, Boca Raton.
- Kafel A, Bednarska K, Augustyniak M, Witas I, Szulinska E (2003) Activity of glutathione S-transferase in Spodoptera exigua larvae exposed to cadmium and zinc in two subsequent generations. Environment International 28: 683-686.
- Kansu U, Uğur A (1984) Pimpla turionellae (L.) (Hym. Ichneumonide) ile konukçusu bazı lepidopter türleri arasındaki biyolojik ilişkiler üzerine araştırmalar. Doğa Bilimleri Dergisi 8: 160-173.
- Kazmirowa M, Ortel J (2000) Metal accumulation by Ceratitis capitata (Diptera) and transfer to the parasitic wasp Coptera occidentalis (Hymenoptera). Environmental Toxicology and Chemistry 7: 1822-1830.
- Lagadic L, Caquet T, Ramade F (1994) The role of biomarkers in environmental assessment. Invertebrate Populations and Communities. Ecotoxicology 3 (5): 193-208.
- Laskowski R (2001) Why short-termbioassays are not meaningful-effects of a pesticide (imidacloprid) and a metal (cadmium) on pea aphids (Acyrthosiphon pisum Harris). Ecotoxicology 10: 177-183.
- Lee JC, Andow DA, Heimpel GE (2006) Influence of floral resources on sugar feeding and nutrient dynamics of a parasitoid in the field. Ecological Entomology 31: 470-480.
- Lijun L, Xuemeia L, Yaping G, Enbo M (2005) Activity of enyzmes of the antioxidative system in cadmiumtreated Oxya chinensis (Orthoptera: Acridoidae). Environmental toxicology and Pharmacology 20: 412-416.
- Linde AR, Arribas P, Sanchez-Galon S, Garcia-Vazguez F (1996) Eel (Anguila anguila) and Brown trout (Salma trutta) target species to assess the biological impact of trace metal pollution in freshwater ecosystems. Archives of Environmental Contamination and Toxicology 31: 297-302.
- Martinez M, Torreblanca A, Ramo JD, Diaz-Mayans J (1994) Effects of sublethal exposure to lead on levels of energetic compound in Procambarus clarkii. Bulletin of Environmental Contamination Toxicology 52: 729-733.
- McMurray CT, Tainer JA (2003) Cancer, cadmium and genome integrity. Nature Genetic 34: 239-241.
- Mikhailova MV, Littlefield NA, Hass BS, Poirier LA, Chou MW (1997) Cadmium-induced 8- hydroxydeoxyguanosine formation, DNA strand breaks and antioxidant enzyme activities in lymphoblastoid cells. Cancer Letters 115: 141-148.
- Moura G, Vilarinho L, Machado J (2000) The action of Cd, Cu, Cr, Zn and Pb on fluid composition of Anodonta cygnea (L.): Organic components. Comperative Biochemisrty and Physiology Part B, 127 (1): 105-112.
- Norton KB, Kench JE (1977) Effects of cadmium on ribosomal protein synthesis in rat liver. Environmental Research 13: 102-110.
- Nummelin M, Lodenius M, Tulisalo E, Hirvonen H, Alanko T (2007) Predatory insects as bioindicators of heavy metal pollution. Environmental Pollution 145: 339-347.
- Olson DM, Fadamiro H, Lundgren JG, Heimpel GE (2000) Effects of sugar feeding on carbohydrate and lipid metabolism in a parasitoid wasp. Physiological Entomology 25: 17-26.
- Ortel J (1991) Effects of lead and cadmium on chemical composition and total water content of the pupal parasitoid, Pimpla turionellae. Entomologia Experimentalis et Applicata 59: 93-100.
- Ortel J (1996) Metal-supplemented diets alter carbohydrate levels in tissue and hemolymph of gypsy moth larvae (Lymantria dispar, Lymantriidae, Lepidoptera). Environmental Toxicology and Chemistry 15: 1171-1176.
- Planello R, Martinez-Guitarte JL, Morcillo G (2010) Effect of acute exposure to cadmium on the expression of heat-shock and hormone-nuclear receptor genes in the aquatic midge Chironomus riparius. Science of the Total Environment 408: 1598-1603.
- Plummer DI (1971) An Introduction to Practical Biochemistry. Mc Graw Hill Book Company, London. Rathore RS, Sanghui PK, Swarup H (1979) Toxicity of cadmium chlorid and lead nitrate to Chronomus tentans larvae. Environmental Pollution 18: 173-177.
- Roe HJ, Batley JM, Gray RR, Robinson JN (1961) Complete removal of glycogen from tissues by extraction with cold trichloroacetic acid solution. Journal of Biological Chemistry 236: 1224-1246
- Sancho E, Ferrando MD, Fernandez C, Andreu E (1998) Liver energy metabolism of Anguilla anguilla after exposure of fenitrothion. Ecotoxicolgy and Environmental Safety 41: 168-175.
- Schmidt GH, Ibrahim NMM, Abdallah MD (1992) Long term effects of heavy metals in food on developmental stages of Aiolopus thalassinus (Saltatoria:Acrididae). Archives of Environmental Contamination and Toxicoloy 23: 375-382.
- Schmidt GH, Ibrahim NMM, Abdallah MD (1991) Toxicological studies on the long-termeffects of heavy metals (Hg, Cd, Pb) in soil on the development of Aiolopus thalassinus (Fabr.) (Saltatoria: Acrididae). The Science of the Total Environment 107: 109-134.
- Sildanchandra W, Crane M (2000) Influence of sexual dimorphism in Chironomus riparius Meigen on toxic effects of cadmium. Environmental Toxicology and Chemistry 19: 2309-2313.
- Spring JH, Matthews JR, Downer A (1977) Fate of glucose in the haemolymph of the American cockroach, Periplaneta americana. Journal of Insect Physiology 23: 525-529.
- Tang N, Enger MD (1993) Cd(2+) induced c-myc mRNA accumulation in NRK-49F cells is blocked by the protein kinase inhibitor H7 but not by HA1004, indicating that protein kinase C is a mediator of the response. Toxicology 81(2):155–164
- Telang A, Buck NA, Wheeler DE (2002) Response of storage protein levels to variation in dietary protein levels. Journal of Insect Physiology 48 (11): 1021-1029.
- Uckan F, Sak O (2010) Cytotoxic Effect of Cypermethrin on Pimpla turionellae (Hymenoptera: Ichneumonidae) Larval Hemocytes. Ekoloji 19 (75): 20-26.
- Van Oik T, Rantala MJ, Saloniemi I (2007) Diet-mediated effects of heavy metal pollution on growth and immune response in the geometrid moth Epirrita autumnata. Environmental Pollution 145: 348-354.
- Wackers FL, Lee JC, Heimpel GE, Winkler K, Wagenaar R (2006) Hymenopteran parasitoids synthesize ‘honeydew-specific’ oligosaccharides. Functional Ecology 20: 790-798.
- Wilczek G, Babczynska A, Augustyniak M, Migula P (2004) Relations between metals (Zn, Pb, Cd and Cu) and glutathione-dependent detoxifying enzymes in spiders from a heavy metal pollution gradient. Environmental Pollution 132: 453–461.
- Wilps H, Gade G (1990) Hormonal regulation of carbohydrate metabolism in the blowfly Phormia terraenovae. Journal of lnsect Physiology 36: 441-449.
- Wu GX, Ye GY, Hu C, Cheng JA (2006) Accumulation of cadmium and its effects on growth, development and hemolymph biochemical compositions in Boettcherisca peregrina larvae (Diptera:Sarcophagidae). Insect Science 13: 31-39.
- Zaba BN, Harris EJ (1978) Accumulation and effects of trace metal ions in fish Liver Mitochondria. Comparative Biochemistry and Physiology 61C (1): 57-66.
- Zhang ZS, Lu XG, Wang CQ, Zheng DM (2009) Mercury, cadmium and lead biogeochemistry in the soilplant- insect in Hulado City. Bulletin of Environmental Contamination and Toxicology 83: 255-259.
- Zheng DM, Wang QC, Zhang ZS, Zheng N, Zhang XW (2008) Bioaccumulation of total and methyl mercury by arthropods. Bulletin of Environmental Contamination and Toxicology 81: 95-100.
| APA | KAYIŞ T, EMRE İ (2012). Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. , 61 - 67. |
| Chicago | KAYIŞ Tamer,EMRE İskender Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. (2012): 61 - 67. |
| MLA | KAYIŞ Tamer,EMRE İskender Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. , 2012, ss.61 - 67. |
| AMA | KAYIŞ T,EMRE İ Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. . 2012; 61 - 67. |
| Vancouver | KAYIŞ T,EMRE İ Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. . 2012; 61 - 67. |
| IEEE | KAYIŞ T,EMRE İ "Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri." , ss.61 - 67, 2012. |
| ISNAD | KAYIŞ, Tamer - EMRE, İskender. "Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri". (2012), 61-67. |
| APA | KAYIŞ T, EMRE İ (2012). Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. Ekoloji, 21(83), 61 - 67. |
| Chicago | KAYIŞ Tamer,EMRE İskender Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. Ekoloji 21, no.83 (2012): 61 - 67. |
| MLA | KAYIŞ Tamer,EMRE İskender Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. Ekoloji, vol.21, no.83, 2012, ss.61 - 67. |
| AMA | KAYIŞ T,EMRE İ Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. Ekoloji. 2012; 21(83): 61 - 67. |
| Vancouver | KAYIŞ T,EMRE İ Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri. Ekoloji. 2012; 21(83): 61 - 67. |
| IEEE | KAYIŞ T,EMRE İ "Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri." Ekoloji, 21, ss.61 - 67, 2012. |
| ISNAD | KAYIŞ, Tamer - EMRE, İskender. "Ağır metal stresinin Pimpla turionellae (Hymenoptera: Ichneumonidae)’nin protein ve glikojen sentezine etkileri". Ekoloji 21/83 (2012), 61-67. |
