In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation

AHISHALI, Bülent; BILIR, AYHAN; EKİNCİ, AYSUN; EKİNCİ, CENAP

In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation

Cenap EKİNCİ, (Diyarbakır Kadın Doğum ve Çocuk Hastalıkları Hastanesi, Diyarbakır, Türkiye)

Aysun EKİNCİ, (Diyarbakır Kadın Doğum ve Çocuk Hastalıkları Hastanesi, Diyarbakır, Türkiye)

Bülent AHISHALI, (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İstanbul, Türkiye)

Ayhan BİLİR (İstanbul Üniversitesi, İstanbul Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İstanbul, Türkiye)

Journal of Clinical and Experimental Investigations

1 1

Yıl: 2012 Cilt: 3 Sayı: 3 Sayfa Aralığı: 350 - 356 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation

Öz:

Amaç: Elajik asit bitkilerden elde edilen polifenolik bir bileşiktir. Bu çalışmanın amacı, elajik asidin sıçan C6 glioma hücre dizilerinde proliferasyon ve yaşama yeteneği üzerine in vitro etkilerini doz ve zamana bağlı olarak araştırmak ve C6 glioma küremsi cisimlerinde oluşan elektronmikroskopik değişiklikleri değerlendirmektir. Gereç ve yöntem: Elajik asidin sıçan C6 glioma hücre dizisi üzerine etkileri doz ve zamana bağlı olarak iki boyutlu tümör hücre kültürü kullanılarak araştırıldı. Elajik asidin etkileri için üç boyutlu hücre kültürü küremsi modellerinde; hücre çoğalması, hücre yaşamsallığı, hücre döngüsünün sentez fazı ve hücre yapılarının 24, 48 ve 72. saatlerdeki durumu değerlendirildi. Bulgular: İki boyutlu hücre kültürlerinde hücre yaşama yeteneği ve hücre çoğalması üzerinde elajik asidin baskılayıcı etkisi görüldü (p<0.05). İki boyutlu hücre kültürlerinde hücre sentez fazında artma veya azalma gözlenmedi (p>0.05). Üç boyutlu hücre kültürü küremsi modellerinde, elektronmikroskopik incelemede kontrol grubundan farklı yapısal değişiklikler bulundu. Sonuç: İki boyutlu kültür modelleri kullanıldığında, elajik asidin sıçan C6 glioma hücre kültürleri üzerine olan etkilerinin değerlendirilmesi sonucunda; elajik asidin hücre yaşayabilirliğini azalttığı, hücre yapısında bozulmalara neden olduğu ve hücre çoğalmasını engellediği bulundu.

Anahtar Kelime:

Konular: Cerrahi

Elajik asidin sıçan C6 glioma hücre kültürlerinde sitotoksisite ve proliferasyon üzerine in vitro etkileri

Öz:

Objectives: Ellagic acid is a plant-derived polyphenolic compound. The aim of this study is to investigate in vitro effects of ellagic acid on the proliferation and viability of rat C6 glioma cell lineage depending on dose and time and also is to evaluate ultrastructural changes in C6 glioma spheroids. Materials and methods: Effects of ellagic acid on rat C6 glioma cell line were investigated by using two-dimensional models of tumor cell culture depending on dose and time. The effects of Ellagic acid was evaluated as cell proliferation, cell viability, and synthesis phase of cell cycle, and cell structure at 24th, 48th, 72nd hours. Cell structure was evaluated at 24th and 72nd hours in three dimensional cell culture spheroid models. Results: Reducing effect of ellagic acid on cell proliferation and cell viability was seen in two dimensional cultures (p<0.05). Increment or decrement were not seen in synthesis phase in two dimensional cultures (p>0. 05). Electron micrographs in which three dimensional cell culture spheroid models was investigated, structural changes was found to be different from the control group. Conclusions: In the evaluation of effects of ellagic acid on rat C6 glioma cells using two-dimensional culture models, it was observed that ellagic acid caused reduced cell viability, deterioration in cell structure and prevented cell proliferation.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1. Karakaya S. Bioavailability of phenolic compouns. Crit Rev Food Sci Nutr 2004;44(4):453-64.
2. Yang CS, Landau JM, Huang MT, Newmark HL. Inhibition of carcinogenesis by dietary polyphenolic compounds, Ann Rev Nutr 2001:21(3):381-406.
3. Navinda P, Seeram, Lynn S, et al. In vitro antiproliferative apoptotic and antioxidant activities of punicalagin, ellagic acid and total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 2005:6(2);360-3.
4. Quinones-Hinojasa A, Sanai N, Smith JS, McDermott MW. Techniques to assess proliferative potential of brain tumors. J Neurooncol 2005:74(1);19-30.
5. Vekiari A, Gordon M.H, Garcia-Macias P, Labrinea H. Extraction and determination of ellagic acid contentin chesnut bark and fruit. Food Chemistry 2008:110(4);1007-11.
6. Falsaperla M, Morgia G, Tartarone A, Ardito R, Romano G. Support ellagic acid therapy in patients with hormone refractory prostate cancer (HRPC) on standard chemotherapy using vinorelbine and estramustine phosphate. Eur Urol 2005:47(3);449-55.
7. Strati A, Papoutsi Z, Lianidou E, Moutsatsou P. Effect of ellagic acid on the expression of human telomerase reverse transcriptase (h TERT) α+β+ transcript in estrogen receptor-positive MCF-7 breast cancer cells. Clin Biochem 2009:42(13);1358-62.
8. Alexander C, Whitley G, Stoner D, Darby M, Vale T. Intestinal epithelial cell accumulation of the cancer preventive polyphenol ellagic cid-extensive binding to proteinamd DNA. Biochem Pharmocol 2003:66(8);907-15.
9. Sungwoo Kim, Mostafa W. Gaber, et al. The inhibition of glioma growth in vitro and in vivo by chitoan/ ellagic acid composite biomaterial, Biomaterials 2009:30(27);4743-51.
10. Narayanan BA, Geoffroy O, Willingham MC, Re GG, Nixon DW. p53/p21(WAF1/CIP1) exspression and its possible role in G1 arrest and apoptosis in ellagic acid treated cancer cell, Cancer Lett 1999:136(2);215-21.
11. Larrosa M, Tomas-Barberan FA, Espin JC. The dietary hydrlysable tannin punicalagin releases ellagic acid that induces apoptosis in human colon adenocarcinoma Caco-2 cells by using the mitochondrial pathway. J Nutr Biochem 2006:17(5);611-25.
12. Fjaeraa C, Nanberg E. Effect of ellagic acid on proliferasyon, cell adhesion an apoptosis İn SHSY5Y nuroblastoma cells, Biomed & Pharmacother 2009:63(2);254-61.
13. Vermeulen K, Berneman ZN, vanBockstaele DR. Cell cycle and apoptosis. Cell Prolif 2003:36(1);165-75.
14. Susanne U. Mertents-Talcott, Susan S. Percival. Ellagic acid and quercetin interact synergistically with reveratrol in the induction of apoptosis and cause transient cel cycle arrest in human leukemia cells. Cancer Lett 2005:218(2);141-51.
15. Li TM, Chen GW, Su CC, et al. Ellagic acid induced p53/p21expresion, G1 arrest and apoptozis in human bladder cancer T24 cells. Anticancer Res 2005:25(8);971-9.
16. Tasaki M, Umemura T, Maeda M, et al. Safety assesment of ellagic acid, a food additive, in a subchronic toxicity study using F344 rats. Food Chem Toxicol 2008:46(9);1119-24.
17. Sharma G, Italia JL, Sonaje K, Tikoo K, Ravi Kumar MNV. Biodegradale in situ gelling system for subcutaneous administration of ellagic acid and ellagic acid loaded nanoparticles: evaloation of their antioxidant potantial against cycosporine induced nephrotoxicity in rats. J Control Release 2007:118(1);27-37.
18. Carlsson J, Yuhas JM. Liquid-overlay culture o cellular spheroids: Resent results. Cancer Res 1984:95(1);1-23.
19. Santini MT, Gabriella R. Three dimensional spheroid models in tumor biology. Pathobiology 1999;67(1);148-57.

APA	EKİNCİ C, EKİNCİ A, AHISHALI B, BILIR A (2012). In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. , 350 - 356.
Chicago	EKİNCİ CENAP,EKİNCİ AYSUN,AHISHALI Bülent,BILIR AYHAN In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. (2012): 350 - 356.
MLA	EKİNCİ CENAP,EKİNCİ AYSUN,AHISHALI Bülent,BILIR AYHAN In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. , 2012, ss.350 - 356.
AMA	EKİNCİ C,EKİNCİ A,AHISHALI B,BILIR A In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. . 2012; 350 - 356.
Vancouver	EKİNCİ C,EKİNCİ A,AHISHALI B,BILIR A In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. . 2012; 350 - 356.
IEEE	EKİNCİ C,EKİNCİ A,AHISHALI B,BILIR A "In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation." , ss.350 - 356, 2012.
ISNAD	EKİNCİ, CENAP vd. "In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation". (2012), 350-356.

APA	EKİNCİ C, EKİNCİ A, AHISHALI B, BILIR A (2012). In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. Journal of Clinical and Experimental Investigations, 3(3), 350 - 356.
Chicago	EKİNCİ CENAP,EKİNCİ AYSUN,AHISHALI Bülent,BILIR AYHAN In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. Journal of Clinical and Experimental Investigations 3, no.3 (2012): 350 - 356.
MLA	EKİNCİ CENAP,EKİNCİ AYSUN,AHISHALI Bülent,BILIR AYHAN In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. Journal of Clinical and Experimental Investigations, vol.3, no.3, 2012, ss.350 - 356.
AMA	EKİNCİ C,EKİNCİ A,AHISHALI B,BILIR A In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. Journal of Clinical and Experimental Investigations. 2012; 3(3): 350 - 356.
Vancouver	EKİNCİ C,EKİNCİ A,AHISHALI B,BILIR A In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation. Journal of Clinical and Experimental Investigations. 2012; 3(3): 350 - 356.
IEEE	EKİNCİ C,EKİNCİ A,AHISHALI B,BILIR A "In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation." Journal of Clinical and Experimental Investigations, 3, ss.350 - 356, 2012.
ISNAD	EKİNCİ, CENAP vd. "In vitro effects of ellagic acid in C6 rat glioma cell cultures in terms of cytotoxicity and proliferation". Journal of Clinical and Experimental Investigations 3/3 (2012), 350-356.