Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats

DEMİRAY BAKTI, Şirin; ÇAVUŞOĞLU, Türker; AKARCA, Saadet Özen; OLTULU, Fatih; YAVAŞOĞLU, Altuğ; Aktug, Huseyin; Kosova, Buket; Bozok Cetintas, Vildan

Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats

Hüseyin AKTUĞ, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

VİLDAN BOZOK ÇETİNTAŞ, (Ege Üniversitesi, Tıp Fakültesi, Medikal Biyoloji Bölümü, İzmir, Türkiye)

Buket KOSOVA, (Ege Üniversitesi, Tıp Fakültesi, Medikal Biyoloji Bölümü, İzmir, Türkiye)

Fatih OLTULU, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Şirin DEMİRAY BAKTI, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Türker ÇAVUŞOĞLU, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Saadet Özen AKARCA, (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Altuğ YAVAŞOĞLU (Ege Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, İzmir, Türkiye)

Turkish Journal of Medical Sciences

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Yıl: 2012 Cilt: 42 Sayı: 5 Sayfa Aralığı: 830 - 838 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats

Öz:

Aim: To evaluate the effects of diabetes on nitric oxide synthase (NOS) activity and on gene expressions of B-cell lymphoma protein 2 (Bcl-2) and caspase-3 in the renal tissues of streptozotocin (STZ)-induced rats. Materials and methods: Twenty rats were analyzed in 2 equally distributed groups of healthy controls and STZ-induced diabetic rats. Rats with blood glucose levels of ≥250 mg/dL were considered diabetic. Renal tissue sections were analyzed histomorphologically and immunohistochemically. Gene expressions of caspase-3 and Bcl-2 were evaluated by realtime quantitative polymerase chain reaction. Results: Bcl-2 expression significantly decreased in the diabetic group, both at the gene and protein level. Meanwhile, the expression of caspase-3 increased in the diabetic group, especially in proximal renal tubule cells. However, endothelial NOS (eNOS) protein expression in the glomerular endothelial nuclei was stronger in the control group, and protein expression of inducible NOS (iNOS) increased in the renal medullar area in the diabetic group. Conclusion: According to our results, hyperglycemia inhibits eNOS, leading to reduced nitric oxide production in endothelial cells. Inhibition of eNOS was correlated with glomerular cell loss due to apoptosis. Furthermore, increased expression of iNOS was associated with endothelial dysfunction in STZ-induced diabetic rats. The structure most resistant to diabetes-induced damage was the glomerulus, while the renal medullar and proximal renal tubule cells displayed damage and cicatrization.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Aktug H, Bozok Cetintas V, Kosova B, OLTULU F, DEMİRAY BAKTI Ş, ÇAVUŞOĞLU T, AKARCA S, YAVAŞOĞLU A (2012). Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. , 830 - 838.
Chicago	Aktug Huseyin,Bozok Cetintas Vildan,Kosova Buket,OLTULU Fatih,DEMİRAY BAKTI Şirin,ÇAVUŞOĞLU Türker,AKARCA Saadet Özen,YAVAŞOĞLU Altuğ Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. (2012): 830 - 838.
MLA	Aktug Huseyin,Bozok Cetintas Vildan,Kosova Buket,OLTULU Fatih,DEMİRAY BAKTI Şirin,ÇAVUŞOĞLU Türker,AKARCA Saadet Özen,YAVAŞOĞLU Altuğ Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. , 2012, ss.830 - 838.
AMA	Aktug H,Bozok Cetintas V,Kosova B,OLTULU F,DEMİRAY BAKTI Ş,ÇAVUŞOĞLU T,AKARCA S,YAVAŞOĞLU A Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. . 2012; 830 - 838.
Vancouver	Aktug H,Bozok Cetintas V,Kosova B,OLTULU F,DEMİRAY BAKTI Ş,ÇAVUŞOĞLU T,AKARCA S,YAVAŞOĞLU A Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. . 2012; 830 - 838.
IEEE	Aktug H,Bozok Cetintas V,Kosova B,OLTULU F,DEMİRAY BAKTI Ş,ÇAVUŞOĞLU T,AKARCA S,YAVAŞOĞLU A "Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats." , ss.830 - 838, 2012.
ISNAD	Aktug, Huseyin vd. "Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats". (2012), 830-838.

APA	Aktug H, Bozok Cetintas V, Kosova B, OLTULU F, DEMİRAY BAKTI Ş, ÇAVUŞOĞLU T, AKARCA S, YAVAŞOĞLU A (2012). Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences, 42(5), 830 - 838.
Chicago	Aktug Huseyin,Bozok Cetintas Vildan,Kosova Buket,OLTULU Fatih,DEMİRAY BAKTI Şirin,ÇAVUŞOĞLU Türker,AKARCA Saadet Özen,YAVAŞOĞLU Altuğ Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences 42, no.5 (2012): 830 - 838.
MLA	Aktug Huseyin,Bozok Cetintas Vildan,Kosova Buket,OLTULU Fatih,DEMİRAY BAKTI Şirin,ÇAVUŞOĞLU Türker,AKARCA Saadet Özen,YAVAŞOĞLU Altuğ Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences, vol.42, no.5, 2012, ss.830 - 838.
AMA	Aktug H,Bozok Cetintas V,Kosova B,OLTULU F,DEMİRAY BAKTI Ş,ÇAVUŞOĞLU T,AKARCA S,YAVAŞOĞLU A Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences. 2012; 42(5): 830 - 838.
Vancouver	Aktug H,Bozok Cetintas V,Kosova B,OLTULU F,DEMİRAY BAKTI Ş,ÇAVUŞOĞLU T,AKARCA S,YAVAŞOĞLU A Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats. Turkish Journal of Medical Sciences. 2012; 42(5): 830 - 838.
IEEE	Aktug H,Bozok Cetintas V,Kosova B,OLTULU F,DEMİRAY BAKTI Ş,ÇAVUŞOĞLU T,AKARCA S,YAVAŞOĞLU A "Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats." Turkish Journal of Medical Sciences, 42, ss.830 - 838, 2012.
ISNAD	Aktug, Huseyin vd. "Dysregulation of nitric oxide synthase activity and Bcl-2 and caspase-3 gene expressions in renal tissue of streptozotocin-induced diabetic rats". Turkish Journal of Medical Sciences 42/5 (2012), 830-838.