The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application

ÖZEN, Alaattin; USTA, Ufuk; SEZER, Atakan; Süt, Necdet; UZAL, Cem; YAĞCI, Mehmet Ali

The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application

Cem UZAL, (Trakya Üniversitesi, Tıp Fakültesi, Radyasyon Onkolojisi Anabilim Dalı, Edirne, Türkiye)

Atakan SEZER, (Trakya Üniversitesi, Tıp Fakültesi, Radyasyon Onkolojisi Anabilim Dalı, Edirne, Türkiye)

Ufuk USTA, (Trakya Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Edirne, Türkiye)

Necdet SÜT, (Trakya Üniversitesi, Tıp Fakültesi, Biyoistatistik Anabilim Dalı, Edirne, Türkiye)

Alaattin ÖZEN, (Trakya Üniversitesi, Tıp Fakültesi, Radyasyon Onkolojisi Anabilim Dalı, Edirne, Türkiye)

Mehmet Ali YAĞCI (Trakya Üniversitesi, Tıp Fakültesi, Cerrahi Anabilim Dalı, Edirne, Türkiye)

Balkan Medical Journal

1 0

Yıl: 2012 Cilt: 29 Sayı: 1 Sayfa Aralığı: 32 - 38 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application

Öz:

Amaç: Bu çalışmanın amacı ışınlama ile oluşturulan proktitte amifostinin radyoprotektif etkinliğinin intrarektal ve intraperitoneal uygulama yapılarak karşılaştırılmasıdır. Hastalar ve Yöntemler: Otuz iki Sprague-Dawley sıçan randomize olarak 4 gruba ayrıldı: Kontrol (CONT), sadece ışınlama (RT), intraperitoneal amifostin ve ışınlama (IPAMI) ile intrarektal amifostin ve ışınlama (IRAMI). RT, IPAMI ve IRAMI gruplarındaki sıçanların her birinin pelvis bölgesi tek fraksiyonda 17,5 Gy ışınlandı. Amifostin ışınlamadan önce intraperitoneal (200 mg/kg) veya intrarektal (2000 mg/kg) yoldan uygulandı. Rektumun histopatolojik incelemesi ışınlamadan 14 gün sonra yapıldı. Bulgular: Bütün histopatolojik parametrelerde ışınlamanın istatistiksel olarak anlamlı hasar oluşturduğu ve bu hasarın amifostin uygulaması ile azaldığı gözlendi. Işınlamadan önce IPAMI uygulaması lamina propria inflamasyonunu (p=0.021), kriptiti (p=0.002), kript absesini (p=0.015) anlamlı olarak azalttı. Buna karşın IRAMI ile koruyucu etki eozinofil lökosit sayısı (p=0.02), kript distorsiyonu (p=0.013) ve rejeneratif/reperatif atipi (p=0.013) dahil tüm parametreler için anlamlı oldu ve anlamlılıkları IPAMI’ya göre eşit veya daha yüksekti. Sonuç: Amifostinin yüksek doz intrarektal topikal uygulaması ışınlamanın oluşturduğu proktitin önlenmesinde intraperitoneal sistemik uygulamasına göre daha etkindir. Anahtar sözcükler: Amifostin, proktit, ışınlama, radyoproteksiyon.

Anahtar Kelime:

Konular: Cerrahi

Radyasyon kaynaklı proktitte amifostinin koruyucu etkinliği: Sistemik ile topikal uygulamaların karşılaştırılması

Öz:

Objective: The aim of the study was to evaluate the radioprotective efficacy of intrarectal administration of amifostine in radiation-induced proctitis compared to intraperitoneal administration. Materials and Methods: Thirty-two Sprague-Dawley rats were randomly divided into four groups: Control (CONT), irradiation alone (RT), intraperitoneal amifostine plus irradiation (IPAMI), and intrarectal amifostine plus irradiation (IRAMI). The rats in the RT, IPAMI and IRAMI groups were irradiated individually with a single dose of 17.5 Gy to the pelvis. Amifostine was administered by the intraperitoneal (200 mg/kg) or intrarectal (2000 mg/kg) route before irradiation. Histopathologic analysis of the rectum was performed 14 days after irradiation. Results: Significant radiation damage appeared in all histopathologic parameters and was reduced by amifostine. Pretreatment with IPAMI significantly reduced the inflammatory infiltrate in the lamina propria (p=0.021), cryptitis (p=0.002) and crypt abscess (p=0.015). However, the protective effect of IRAMI was significant for all parameters with equal or higher significance than IPAMI, including the eosinophil leucocytes count (p=0.02), and distortion of the crypts (p=0.008), and was also significant for regenerative/reparative atypia (p=0.013). Conclusion: Intrarectal high dose topical administration of amifostine is more effective in the prevention of radiation-induced proctitis compared to its intraperitoneal systemic administration.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	UZAL C, SEZER A, USTA U, Süt N, ÖZEN A, YAĞCI M (2012). The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. , 32 - 38.
Chicago	UZAL Cem,SEZER Atakan,USTA Ufuk,Süt Necdet,ÖZEN Alaattin,YAĞCI Mehmet Ali The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. (2012): 32 - 38.
MLA	UZAL Cem,SEZER Atakan,USTA Ufuk,Süt Necdet,ÖZEN Alaattin,YAĞCI Mehmet Ali The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. , 2012, ss.32 - 38.
AMA	UZAL C,SEZER A,USTA U,Süt N,ÖZEN A,YAĞCI M The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. . 2012; 32 - 38.
Vancouver	UZAL C,SEZER A,USTA U,Süt N,ÖZEN A,YAĞCI M The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. . 2012; 32 - 38.
IEEE	UZAL C,SEZER A,USTA U,Süt N,ÖZEN A,YAĞCI M "The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application." , ss.32 - 38, 2012.
ISNAD	UZAL, Cem vd. "The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application". (2012), 32-38.

APA	UZAL C, SEZER A, USTA U, Süt N, ÖZEN A, YAĞCI M (2012). The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. Balkan Medical Journal, 29(1), 32 - 38.
Chicago	UZAL Cem,SEZER Atakan,USTA Ufuk,Süt Necdet,ÖZEN Alaattin,YAĞCI Mehmet Ali The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. Balkan Medical Journal 29, no.1 (2012): 32 - 38.
MLA	UZAL Cem,SEZER Atakan,USTA Ufuk,Süt Necdet,ÖZEN Alaattin,YAĞCI Mehmet Ali The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. Balkan Medical Journal, vol.29, no.1, 2012, ss.32 - 38.
AMA	UZAL C,SEZER A,USTA U,Süt N,ÖZEN A,YAĞCI M The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. Balkan Medical Journal. 2012; 29(1): 32 - 38.
Vancouver	UZAL C,SEZER A,USTA U,Süt N,ÖZEN A,YAĞCI M The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application. Balkan Medical Journal. 2012; 29(1): 32 - 38.
IEEE	UZAL C,SEZER A,USTA U,Süt N,ÖZEN A,YAĞCI M "The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application." Balkan Medical Journal, 29, ss.32 - 38, 2012.
ISNAD	UZAL, Cem vd. "The protective effect of amifostine on radiation-induced proctitis: Systemic versus topical application". Balkan Medical Journal 29/1 (2012), 32-38.