Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model

Fırat, Tülin; KUKNER, Aysel; ÇAKICI, Hüsamettin; YÜKSEL, HALİL YALÇIN; HAPA, Onur; AYGÜN, Hayati

Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model

Onur HAPA, (Dokuz Eylül Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmatoloji Anabilim Dalı, İzmir, Türkiye)

Hüsamettin ÇAKICI, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Ortopedi ve Tramatoloji Anabilim Dalı, Bolu, Türkiye)

Halil Yalçın YÜKSEL, (Ankara Numune Eğitim ve Araştırma Hastanesi, Ortopedi ve Travmatoloji Kliniği, Ankara, Türkiye)

Tülin FIRAT, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bolu, Türkiye)

Aysel KÜKNER, (Abant İzzet Baysal Üniversitesi, Tıp Fakültesi, Histoloji ve Embriyoloji Anabilim Dalı, Bolu, Türkiye)

Hayati AYGÜN (Kafkas Üniversitesi, Tıp Fakültesi, Ortopedi ve Travmataloji Anabilim Dalı, Kars, Türkiye)

Acta Orthopaedica et Traumatologica Turcica

4 0

Yıl: 2013 Cilt: 47 Sayı: 3 Sayfa Aralığı: 201 - 207 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model

Öz:

Amaç: Mevcut çalışmanın amacı trombositten zengin plazma (platelet-rich plasma, PRP) + mikrofraktür ile mikrofraktür tedavilerinin kronik fokal kondral defekt iyileşmesine etkisini karşılaştırmaktı. Çalışma planı: Bu çalışmada 57 adet erişkin erkek Sprague-Dawley sıçanı kullanıldı. Kırk iki sıçan kondral defekt oluşturulduktan sonra 14’erlikten 3 gruba (kontrol, sadece mikrofraktür, PRP+mikrofraktür) ayrıldı. Kalan 15 sıçan PRP hazırlanmasında kullanıldı. Tedaviden 3 ve 6 hafta sonra sıçanlara ötenazi uygulandı ve incelemeleri yapıldı. Uyarlanmış Pineda skorlama sistemi ile histolojik analiz ve Tip 2 kollajen için immünohistokimyasal boyama yapıldı. Bulgular: Her iki zaman aralığında da, kontrol grubu histolojik skorları (3. hafta: 8.8±1.2, 6. hafta: 8.5±0.7) mikrofraktür (3. hafta: 6.8±1.0, 6. hafta: 7.1±0.6) ve PRP+mikrofraktür (3. hafta: 6.4±1.3, 6. hafta: 5.7±1.2) gruplarından daha yüksekti (p<0.05). Altıncı hafta mikrofraktür grup skoru, 6. hafta PRP+mikrofraktür grubundan daha yüksekti (p<0.05). Tip 2 kollajen boyanma derecesi 6. haftada PRP+mikrofraktür grubunda daha yüksekti ve hücre membranında membranöz boyama gözlendiğinden benzersizdi. Çıkarımlar: Kronik fokal kondral defekt tedavisi için mikrofraktürlerle birlikte PRP uygulamasının kıkırdak iyileşmesini daha etkin kıldığı gözükmektedir.

Anahtar Kelime:

Konular: Cerrahi

Trombositten zengin plazma kronik fokal kondral defektlerin mikrofraktür ile tedavisini daha etkin kılar mı? Sıçan modelli in vivo çalışma

Öz:

Objective: The purpose of the present study was to compare the effectiveness of platelet-rich plasma (PRP) + microfracture and microfracture treatments in the healing of chronic focal chondral defects. Methods: The study included 57 adult male Sprague-Dawley rats. Forty-two rats were divided into three groups of 14 rats with a chondral defect (control, microfracture only, PRP+microfracture). The remaining 15 rats were used to produce the PRP preparation. The rats were then euthanatized at 3 and 6 weeks after treatment and examined. Histological analysis using the modified Pineda scoring system and immunohistochemical staining for Type 2 collagen were performed. Results: At both time intervals, control group histological scores (Week 3: 8.8±1.2, Week 6: 8.5±0.7) were higher than microfracture (Week 3: 6.8±1.0, Week 6: 7.1±0.6) and PRP+microfracture (Week 3: 6.4±1.3, Week 6: 5.7±1.2) scores (p<0.05). The microfracture group score was higher at Week 6 than the PRP+microfracture group (p<0.05). The degree of Type 2 collagen staining was higher at Week 6 in the PRP+microfracture group and was unique in showing staining at the cell membrane. Conclusion: The addition of PRP application to microfracture treatment appears to enhance cartilage healing in chronic focal chondral defects.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Bibliyografik

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APA	HAPA O, ÇAKICI H, YÜKSEL H, Fırat T, KUKNER A, AYGÜN H (2013). Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. , 201 - 207.
Chicago	HAPA Onur,ÇAKICI Hüsamettin,YÜKSEL HALİL YALÇIN,Fırat Tülin,KUKNER Aysel,AYGÜN Hayati Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. (2013): 201 - 207.
MLA	HAPA Onur,ÇAKICI Hüsamettin,YÜKSEL HALİL YALÇIN,Fırat Tülin,KUKNER Aysel,AYGÜN Hayati Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. , 2013, ss.201 - 207.
AMA	HAPA O,ÇAKICI H,YÜKSEL H,Fırat T,KUKNER A,AYGÜN H Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. . 2013; 201 - 207.
Vancouver	HAPA O,ÇAKICI H,YÜKSEL H,Fırat T,KUKNER A,AYGÜN H Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. . 2013; 201 - 207.
IEEE	HAPA O,ÇAKICI H,YÜKSEL H,Fırat T,KUKNER A,AYGÜN H "Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model." , ss.201 - 207, 2013.
ISNAD	HAPA, Onur vd. "Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model". (2013), 201-207.

APA	HAPA O, ÇAKICI H, YÜKSEL H, Fırat T, KUKNER A, AYGÜN H (2013). Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. Acta Orthopaedica et Traumatologica Turcica, 47(3), 201 - 207.
Chicago	HAPA Onur,ÇAKICI Hüsamettin,YÜKSEL HALİL YALÇIN,Fırat Tülin,KUKNER Aysel,AYGÜN Hayati Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. Acta Orthopaedica et Traumatologica Turcica 47, no.3 (2013): 201 - 207.
MLA	HAPA Onur,ÇAKICI Hüsamettin,YÜKSEL HALİL YALÇIN,Fırat Tülin,KUKNER Aysel,AYGÜN Hayati Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. Acta Orthopaedica et Traumatologica Turcica, vol.47, no.3, 2013, ss.201 - 207.
AMA	HAPA O,ÇAKICI H,YÜKSEL H,Fırat T,KUKNER A,AYGÜN H Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. Acta Orthopaedica et Traumatologica Turcica. 2013; 47(3): 201 - 207.
Vancouver	HAPA O,ÇAKICI H,YÜKSEL H,Fırat T,KUKNER A,AYGÜN H Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model. Acta Orthopaedica et Traumatologica Turcica. 2013; 47(3): 201 - 207.
IEEE	HAPA O,ÇAKICI H,YÜKSEL H,Fırat T,KUKNER A,AYGÜN H "Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model." Acta Orthopaedica et Traumatologica Turcica, 47, ss.201 - 207, 2013.
ISNAD	HAPA, Onur vd. "Does platelet-rich plasma enhance microfracture treatment for chronic focal chondral defects? An in-vivo study performed in a rat model". Acta Orthopaedica et Traumatologica Turcica 47/3 (2013), 201-207.