Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

ÖZMEN, NESRİN; Birhanli, Emre; Yesilada, Ozfer; ERCAN, Sinem

Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Özfer YEŞİLADA, (İnönü Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Malatya, Türkiye)

Emre BİRHANLI, (İnönü Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Malatya, Türkiye)

Sinem ERCAN, (İnönü Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Malatya, Türkiye)

Nesrin ÖZMEN (İnönü Üniversitesi, Eğitim Fakültesi, Malatya, Türkiye)

Turkish Journal of Biology

5 0

Yıl: 2014 Cilt: 38 Sayı: 1 Sayfa Aralığı: 103 - 110 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii

Öz:

The effect of various factors on dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii ATCC 200800 to obtain rapid and high decolorization activity against Reactive Black 5 and Reactive Blue 171 dyes was investigated. All conditions used were important for dye decolorizing activity of this crude laccase enzyme. The optimum pH of decolorization was tested at 30 °C and it was around 3.0. This activity was highly reduced at pH 4.5–6.0. On the other hand, the optimum temperature for rapid and high decolorization was 50 °C. Importantly, the decolorization rate of crude laccase at pH 4.5 and pH 6.0 increased with the rise in temperature from 30 °C to 50 °C. Therefore, high decolorization could be obtained at pH 4.5 and even pH 6.0 by selecting the proper temperature for these pH values. Enzyme amount also affected the dye decolorization positively. This crude laccase could also decolorize the mixed dyes (Reactive Black 5 and Reactive Blue 171) and synthetic wastewaters. Native polyacrylamide gel electrophoresis showed the responsibility of the laccase enzyme in decolorization. Rapid and high textile dye decolorization through the selection of appropriate conditions could facilitate the development of more economical and environmentally friendly processes.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Yesilada O, Birhanli E, ERCAN S, ÖZMEN N (2014). Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. , 103 - 110.
Chicago	Yesilada Ozfer,Birhanli Emre,ERCAN Sinem,ÖZMEN NESRİN Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. (2014): 103 - 110.
MLA	Yesilada Ozfer,Birhanli Emre,ERCAN Sinem,ÖZMEN NESRİN Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. , 2014, ss.103 - 110.
AMA	Yesilada O,Birhanli E,ERCAN S,ÖZMEN N Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. . 2014; 103 - 110.
Vancouver	Yesilada O,Birhanli E,ERCAN S,ÖZMEN N Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. . 2014; 103 - 110.
IEEE	Yesilada O,Birhanli E,ERCAN S,ÖZMEN N "Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii." , ss.103 - 110, 2014.
ISNAD	Yesilada, Ozfer vd. "Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii". (2014), 103-110.

APA	Yesilada O, Birhanli E, ERCAN S, ÖZMEN N (2014). Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. Turkish Journal of Biology, 38(1), 103 - 110.
Chicago	Yesilada Ozfer,Birhanli Emre,ERCAN Sinem,ÖZMEN NESRİN Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. Turkish Journal of Biology 38, no.1 (2014): 103 - 110.
MLA	Yesilada Ozfer,Birhanli Emre,ERCAN Sinem,ÖZMEN NESRİN Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. Turkish Journal of Biology, vol.38, no.1, 2014, ss.103 - 110.
AMA	Yesilada O,Birhanli E,ERCAN S,ÖZMEN N Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. Turkish Journal of Biology. 2014; 38(1): 103 - 110.
Vancouver	Yesilada O,Birhanli E,ERCAN S,ÖZMEN N Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii. Turkish Journal of Biology. 2014; 38(1): 103 - 110.
IEEE	Yesilada O,Birhanli E,ERCAN S,ÖZMEN N "Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii." Turkish Journal of Biology, 38, ss.103 - 110, 2014.
ISNAD	Yesilada, Ozfer vd. "Reactive dye decolorization activity of crude laccase enzyme from repeated-batch culture of Funalia trogii". Turkish Journal of Biology 38/1 (2014), 103-110.