Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2

SARAL SARIYER, AYSEGÜL; BELDUZ, Ali Osman; ÇOPUR ÇİÇEK, AYŞEGÜL; SANDALLI, Cemal; ÜLKER, Serdar; KARAOGLU, HAKAN

Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2

Cemal SANDALLI, (Recep Tayyip Erdoğan Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Ayşegül SARAL, (Artvin Çoruh Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Artvin, Türkiye)

Serdar ÜLKER, (Recep Tayyip Erdoğan Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Rize, Türkiye)

Hakan KARAOĞLU, (Recep Tayyip Erdoğan Üniversitesi, Su Ürünleri Fakültesi, Rize, Türkiye)

Ali Osman BELDÜZ, (Karadeniz Teknik Üniversitesi, Fen Edebiyat Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)

AYŞEGÜL ÇOPUR ÇİÇEK (Recep Tayyip Erdoğan Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Anabilim Dalı, Rize, Türkiye)

Turkish Journal of Biology

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Yıl: 2014 Cilt: 38 Sayı: 1 Sayfa Aralığı: 111 - 117 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2

Öz:

The cytidine-5’-triphosphate (CTP) synthase (EC 6.4.3.2) gene (pyrG) was cloned and sequenced from the thermophilic bacterium Anoxybacillus gonensis G2 (Ago). The gene is 1590 bp in length and encodes a protein of 530 amino acids, with a molecular mass of 59.5 kDa. The amino acid sequence of CTP synthase shares approximately 90%–94% similarity to Bacillus sp., and it belongs to the triad glutamine amidotransferases, which utilize a Cys–His–Glu triad for activity. Multiple sequence alignments revealed that the enzyme includes conserved amino acids responsible for catalytic activity and the binding of a divalent metal ion (Mg+2). AgoCTP synthase (AgoG2CTPs) was overproduced in Escherichia coli BL21 (DE3) pLysS as recombinant and purified by nickel affinity chromatography. Its biochemical characterization showed that the enzyme had maximal activity at pH 9.0–10.0 and 65 °C. Km, Vmax, and kcat were found to be approximately 12.415 mM, 0.381 U/L, and 0.762 s–1 at 65 °C, respectively. CTP synthase promotes the formation of CTP in dividing cells and is a recognized target for anticancer and antibacterial drugs. The results obtained from this study can be improved upon with the use of different species and substrates.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	SANDALLI C, SARAL SARIYER A, ÜLKER S, KARAOGLU H, BELDUZ A, ÇOPUR ÇİÇEK A (2014). Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. , 111 - 117.
Chicago	SANDALLI Cemal,SARAL SARIYER AYSEGÜL,ÜLKER Serdar,KARAOGLU HAKAN,BELDUZ Ali Osman,ÇOPUR ÇİÇEK AYŞEGÜL Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. (2014): 111 - 117.
MLA	SANDALLI Cemal,SARAL SARIYER AYSEGÜL,ÜLKER Serdar,KARAOGLU HAKAN,BELDUZ Ali Osman,ÇOPUR ÇİÇEK AYŞEGÜL Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. , 2014, ss.111 - 117.
AMA	SANDALLI C,SARAL SARIYER A,ÜLKER S,KARAOGLU H,BELDUZ A,ÇOPUR ÇİÇEK A Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. . 2014; 111 - 117.
Vancouver	SANDALLI C,SARAL SARIYER A,ÜLKER S,KARAOGLU H,BELDUZ A,ÇOPUR ÇİÇEK A Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. . 2014; 111 - 117.
IEEE	SANDALLI C,SARAL SARIYER A,ÜLKER S,KARAOGLU H,BELDUZ A,ÇOPUR ÇİÇEK A "Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2." , ss.111 - 117, 2014.
ISNAD	SANDALLI, Cemal vd. "Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2". (2014), 111-117.

APA	SANDALLI C, SARAL SARIYER A, ÜLKER S, KARAOGLU H, BELDUZ A, ÇOPUR ÇİÇEK A (2014). Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. Turkish Journal of Biology, 38(1), 111 - 117.
Chicago	SANDALLI Cemal,SARAL SARIYER AYSEGÜL,ÜLKER Serdar,KARAOGLU HAKAN,BELDUZ Ali Osman,ÇOPUR ÇİÇEK AYŞEGÜL Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. Turkish Journal of Biology 38, no.1 (2014): 111 - 117.
MLA	SANDALLI Cemal,SARAL SARIYER AYSEGÜL,ÜLKER Serdar,KARAOGLU HAKAN,BELDUZ Ali Osman,ÇOPUR ÇİÇEK AYŞEGÜL Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. Turkish Journal of Biology, vol.38, no.1, 2014, ss.111 - 117.
AMA	SANDALLI C,SARAL SARIYER A,ÜLKER S,KARAOGLU H,BELDUZ A,ÇOPUR ÇİÇEK A Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. Turkish Journal of Biology. 2014; 38(1): 111 - 117.
Vancouver	SANDALLI C,SARAL SARIYER A,ÜLKER S,KARAOGLU H,BELDUZ A,ÇOPUR ÇİÇEK A Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2. Turkish Journal of Biology. 2014; 38(1): 111 - 117.
IEEE	SANDALLI C,SARAL SARIYER A,ÜLKER S,KARAOGLU H,BELDUZ A,ÇOPUR ÇİÇEK A "Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2." Turkish Journal of Biology, 38, ss.111 - 117, 2014.
ISNAD	SANDALLI, Cemal vd. "Cloning, expression, and characterization of a novel CTP synthase gene from Anoxybacillus gonensis G2". Turkish Journal of Biology 38/1 (2014), 111-117.