Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers

SHUNMUGIAH, Karutha Pandian; MUTHIAH, Joe Virgin Largia; MANIKANDAN, Ramesh

Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers

Joe Virgin Largia MUTHIAH, (Department of Biotechnology, Alagappa University, Karaikudi, India)

Karutha Pandian SHUNMUGIAH, (Department of Biotechnology, Alagappa University, Karaikudi, India)

Ramesh MANIKANDAN (Department of Biotechnology, Alagappa University, Karaikudi, India)

Turkish Journal of Botany

4 0

Yıl: 2013 Cilt: 37 Sayı: 6 Sayfa Aralığı: 1008 - 1017 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers

Öz:

The present study has investigated the efficiency of regrowth of Bacopa monnieri (L.) Pennell after 6 months of storage along with its clonal fidelity checking. Shoot tips and nodal segments of in vitro grown plantlets were encapsulated and stored at different temperatures and the highest regeneration was achieved by storing shoot tips at 4 °C for a period of 6 months. The response of shoot tips was found to be much better when compared to nodal segments in the case of forming healthy shoots and roots. The genetic stability of regrown plantlets was analysed by using RAPD and ISSR markers. Twenty ISSR primers produced 130 clear and reproducible amplicons, of which 125 bands were monomorphic. A total of 115 bands were produced by 25 RAPD markers with 94% monomorphism. This corresponds to a very low genetic variation pertaining to the clonal nature of the regrown plantlets with that of the mother plant. By means of HPLC biochemical analysis, bacoside A content in the regrown plants was also found to be comparable with that of the mother plant. Therefore, the protocol derived can be efficiently utilised for storing the encapsulated shoot tips of B. monnieri for about 6 months.

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Konular: Biyoloji

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APA	MUTHIAH J, SHUNMUGIAH K, MANIKANDAN R (2013). Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. , 1008 - 1017.
Chicago	MUTHIAH Joe Virgin Largia,SHUNMUGIAH Karutha Pandian,MANIKANDAN Ramesh Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. (2013): 1008 - 1017.
MLA	MUTHIAH Joe Virgin Largia,SHUNMUGIAH Karutha Pandian,MANIKANDAN Ramesh Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. , 2013, ss.1008 - 1017.
AMA	MUTHIAH J,SHUNMUGIAH K,MANIKANDAN R Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. . 2013; 1008 - 1017.
Vancouver	MUTHIAH J,SHUNMUGIAH K,MANIKANDAN R Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. . 2013; 1008 - 1017.
IEEE	MUTHIAH J,SHUNMUGIAH K,MANIKANDAN R "Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers." , ss.1008 - 1017, 2013.
ISNAD	MUTHIAH, Joe Virgin Largia vd. "Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers". (2013), 1008-1017.

APA	MUTHIAH J, SHUNMUGIAH K, MANIKANDAN R (2013). Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. Turkish Journal of Botany, 37(6), 1008 - 1017.
Chicago	MUTHIAH Joe Virgin Largia,SHUNMUGIAH Karutha Pandian,MANIKANDAN Ramesh Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. Turkish Journal of Botany 37, no.6 (2013): 1008 - 1017.
MLA	MUTHIAH Joe Virgin Largia,SHUNMUGIAH Karutha Pandian,MANIKANDAN Ramesh Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. Turkish Journal of Botany, vol.37, no.6, 2013, ss.1008 - 1017.
AMA	MUTHIAH J,SHUNMUGIAH K,MANIKANDAN R Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. Turkish Journal of Botany. 2013; 37(6): 1008 - 1017.
Vancouver	MUTHIAH J,SHUNMUGIAH K,MANIKANDAN R Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers. Turkish Journal of Botany. 2013; 37(6): 1008 - 1017.
IEEE	MUTHIAH J,SHUNMUGIAH K,MANIKANDAN R "Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers." Turkish Journal of Botany, 37, ss.1008 - 1017, 2013.
ISNAD	MUTHIAH, Joe Virgin Largia vd. "Genetic fidelity assessment of encapsulated in vitro tissues of Bacopa monnieri after 6 months of storage by using ISSR and RAPD markers". Turkish Journal of Botany 37/6 (2013), 1008-1017.