In vitro propagation of endemic and endangered muscari mirum from different explant types

EREN, ÖZKAN; MIRICI, SEMRA; BAKTIR, İbrahim; KARAGÜZEL, Özgül; NASIRCILAR, AYSE GÜL

In vitro propagation of endemic and endangered muscari mirum from different explant types

Ayşe Gül NASIRCILAR, (Akdeniz Üniversitesi, Eğitim Fakültesi, Biyoloji Eğitimi Bölümü, Antalya, Türkiye)

Semra MİRİCİ, (Akdeniz Üniversitesi, Eğitim Fakültesi, İlköğretim Bölümü, Antalya, Türkiye)

Özgül KARAGÜZEL, (Batı Akdeniz Tarımsal Araştırma Enstitüsü, Antalya, Türkiye)

Özkan EREN, (Adnan Menderes Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Aydın, Türkiye)

İbrahim BAKTIR (Akdeniz Üniversitesi, Ziraat Fakültesi, Bahçe Bitkileri Bölümü, Antalya, Türkiye)

Turkish Journal of Botany

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Yıl: 2011 Cilt: 35 Sayı: 1 Sayfa Aralığı: 37 - 43 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

In vitro propagation of endemic and endangered muscari mirum from different explant types

Öz:

Abstract: A protocol for in vitro propagation of Muscari mirum Speta, an endangered geophyte of the family Hyacinthaceae, was developed. Bulb scale, scape, leaf, and immature embryo explants were cultured on different nutrient media compositions supplemented with various concentrations of plant growth regulators (BAP, NAA, TDZ, Picloram, 2,4-D). Scape explants produced callus only. Higher numbers of bulblets were obtained from bulb scale explants than embryo explants. The highest percentage of the bulb formation (23.50 per explant) were obtained from bulb scale explants consisting of 4 scale segments cultured on Murashige and Skoog (MS) medium supplemented with 4 mg $L^{–1} 6-–1 benzylaminopurine (BAP) and 0.25 mg $L^{–1}a naphthaleneacetic acid (NAA) after 5 months of culture initiation. Rooted bulblets which were more than 5 mm in diameter were transplanted into potting mixture of soil, vermiculite and perlite (1:1:1). The chromosome number of the root cells of in vitro induced bulblets was diploid 2n = 18.

Anahtar Kelime:

Konular: Biyoloji

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	NASIRCILAR A, MIRICI S, KARAGÜZEL Ö, EREN Ö, BAKTIR İ (2011). In vitro propagation of endemic and endangered muscari mirum from different explant types. , 37 - 43.
Chicago	NASIRCILAR AYSE GÜL,MIRICI SEMRA,KARAGÜZEL Özgül,EREN ÖZKAN,BAKTIR İbrahim In vitro propagation of endemic and endangered muscari mirum from different explant types. (2011): 37 - 43.
MLA	NASIRCILAR AYSE GÜL,MIRICI SEMRA,KARAGÜZEL Özgül,EREN ÖZKAN,BAKTIR İbrahim In vitro propagation of endemic and endangered muscari mirum from different explant types. , 2011, ss.37 - 43.
AMA	NASIRCILAR A,MIRICI S,KARAGÜZEL Ö,EREN Ö,BAKTIR İ In vitro propagation of endemic and endangered muscari mirum from different explant types. . 2011; 37 - 43.
Vancouver	NASIRCILAR A,MIRICI S,KARAGÜZEL Ö,EREN Ö,BAKTIR İ In vitro propagation of endemic and endangered muscari mirum from different explant types. . 2011; 37 - 43.
IEEE	NASIRCILAR A,MIRICI S,KARAGÜZEL Ö,EREN Ö,BAKTIR İ "In vitro propagation of endemic and endangered muscari mirum from different explant types." , ss.37 - 43, 2011.
ISNAD	NASIRCILAR, AYSE GÜL vd. "In vitro propagation of endemic and endangered muscari mirum from different explant types". (2011), 37-43.

APA	NASIRCILAR A, MIRICI S, KARAGÜZEL Ö, EREN Ö, BAKTIR İ (2011). In vitro propagation of endemic and endangered muscari mirum from different explant types. Turkish Journal of Botany, 35(1), 37 - 43.
Chicago	NASIRCILAR AYSE GÜL,MIRICI SEMRA,KARAGÜZEL Özgül,EREN ÖZKAN,BAKTIR İbrahim In vitro propagation of endemic and endangered muscari mirum from different explant types. Turkish Journal of Botany 35, no.1 (2011): 37 - 43.
MLA	NASIRCILAR AYSE GÜL,MIRICI SEMRA,KARAGÜZEL Özgül,EREN ÖZKAN,BAKTIR İbrahim In vitro propagation of endemic and endangered muscari mirum from different explant types. Turkish Journal of Botany, vol.35, no.1, 2011, ss.37 - 43.
AMA	NASIRCILAR A,MIRICI S,KARAGÜZEL Ö,EREN Ö,BAKTIR İ In vitro propagation of endemic and endangered muscari mirum from different explant types. Turkish Journal of Botany. 2011; 35(1): 37 - 43.
Vancouver	NASIRCILAR A,MIRICI S,KARAGÜZEL Ö,EREN Ö,BAKTIR İ In vitro propagation of endemic and endangered muscari mirum from different explant types. Turkish Journal of Botany. 2011; 35(1): 37 - 43.
IEEE	NASIRCILAR A,MIRICI S,KARAGÜZEL Ö,EREN Ö,BAKTIR İ "In vitro propagation of endemic and endangered muscari mirum from different explant types." Turkish Journal of Botany, 35, ss.37 - 43, 2011.
ISNAD	NASIRCILAR, AYSE GÜL vd. "In vitro propagation of endemic and endangered muscari mirum from different explant types". Turkish Journal of Botany 35/1 (2011), 37-43.