The aim of this study was to identify the bacteria isolated from urine samples and to investigate their antibiotic suscep- tibilities in children aged 0-16 years. Urine samples of 2,983 patients who referred to our hospital between July 2008 and October 2012 and had urinary tract infection symptoms were studied in order to evaluate culture and antibiotic susceptibility testing results. All urine samples were inoculated on eosin methylene blue (EMB) and 5 % blood agars. Bacterial isolates were identified by conventional met- hods. Antibiotic susceptibility testing was performed by disk diffusion method. The bacteria were isolated in 337 (11.3 %) of 2,983 urine samples. The most frequently isolated bacteria (64.4 %) were Escherichia coli. Enterococcus spp. (15.7 %), Proteus spp. (7.1 %), Klebsiella spp. (5.3 %), Enterobacter spp. (5.9 %) and coagulase- negative staphylococci (CNS) (1.5 %) followed respectively. While all E.coli, Proteus spp., Klebsiella spp., and Enterobacter spp. isolates were susceptible to amikacin, gentamicin, and imipenem, sensitivity of the ampicillin (69.5%), and the trimethoprim/sulfa- methoxazole (79.6 %) were the lowest. All Enterococcus spp. (n:53) isolates were susceptible to linezolid and vancomycin, resistan- ce ratios aganist nitrofurantoin, penicillin, and erythromycin were 7.6 %, 17.0 %, and 28.3 % respectively. Although antibiotic susceptibility rates among our hospital’s patient population were higher than other studies, it should be remembered that culture and antimicrobial susceptibility testing may be beneficial to help choosing narrow-spectrum antibio­ ics and to prevent development of resistance.