Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Filiz ÇAKICI ÖZKAN, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Ali SEVİM, (Ahi Evran Üniversitesi, Mühendislik ve Mimarlık Fakültesi, Çevre Mühendisliği, Bölümü, Kırşehir, Türkiye)
Zihni DEMİRBAĞ, (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
İsmail DEMİR (Karadeniz Teknik Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Trabzon, Türkiye)
Turkish Journal of Agriculture and Forestry
2 0
Yıl: 2014 Cilt: 38 Sayı: 1 Sayfa Aralığı: 0 - 0 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents

Öz:
Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is one of the most destructive pests of several vegetables and fruitsworldwide. In spite of various control methods, this pest has still continued to cause signifcant damage. In this study, the culturablebacterial fora of S. littoralis was determined. New isolates from S. littoralis, as well as 12 diferent Bacillus isolates belong to 5 speciesthat were previously isolated from diferent pests, were tested on S. littoralis larvae. In total, 9 bacteria were characterized based ontheir morphological, biochemical, physiological, and molecular characteristics. Te bacterial fora of S. littoralis was determinedas Flavobacterium sp. (SL1), Klebsiella pneumonia (SL2), Enterobacter sp. (SL3), Enterobacter sp. (SL4), Klebsiella sp. (SL5), Serratiamarcescens (SL6), Pseudomonas aeruginosa (SL7), Acinetobacter baumannii (SL8), and Staphylococcus sp. (SL9). Te insecticidal activitytests were performed on the third-instar larvae of S. littoralis. SL1 and SL5 from S. littoralis caused the highest mortalities with 67% and77%, respectively. Among previously isolated Bacillus isolates, Bacillus thuringiensis subsp. kurstaki (MnD) and B. thuringiensis subsp.kurstaki (BnBt) were found to be the most efective, causing 100% mortality within 10 days afer treatment. A concentration-responsetest was conducted with these isolates and it was found that the isolate MnD was more efective than BnBt. Terefore, further bioassayexperiments were conducted with the isolate MnD and results were discussed with respect to the biocontrol potential of the bacterialisolates.
Anahtar Kelime:

Konular: Orman Mühendisliği
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Abbott WS (1925). A method of computing the efectiveness of an insecticide. J Econ Entomol 18: 265–7.
  • Ademolu KO, Idowu AB (2011). Occurrence and distribution of microfora in the gut regions of the variegated grasshopper Zonocerus variegatus (Orthoptera: Pyrgomorphidae) during development. Zool Stud 50: 409–415.
  • Al-Momani F, Obeidat M (2013). Ecology, toxicity, and hydrolytic activities of Bacillus thuringiensis in forests. Turk J Agric For 37: 76–82.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990). Basic local alignment search tool. J Mol Biol 215: 403–410.
  • Banerjee A, Dangar TK (1995). Pseudomonas aeruginosa , a facultative pathogen of red palm weevil, Rhynchophorus ferrugineus. World J Microbiol Biotechol 11: 616–620.
  • Boemare N, Tailliez P (2010). Molecular approaches and techniques for the study of entomopathogenic bacteria. In: Stock SP, Vanderberg J, Boemare N, Glazer I, editors. Insect Pathogens: Molecular Approaches and Techniques. Wallingford, UK: CAB International, pp. 32–45.
  • Bucher GE (1981). Identifcation of bacteria found in insects. In: Burges HD, editor. Microbial Control of Pests and Plant Diseases. New York: Academic Press, pp. 7–33.
  • Consolo VF, Mucci V, Salerno GL, Berón CM (2010). Pathogenicity of bacterial isolates to Cyclocephala signaticollis (Coleoptera: Scarabaeidae). Biocont Sci Technol 20: 475–482.
  • Danışmazoğlu M, Demir İ, Sevim A, Demirbağ Z, Nalçacıoğlu R (2012). An investigation on the bacterial fora of Agriotes lineatus (Coleoptera: Elateridae) and pathogenicity of the fora members. Crop Prot 40: 1–7.
  • Demir İ, Eryüzlü E, Demirbağ Z (2012). A study on the characterization and pathogenicity of the bacteria from Lymantria dispar L. (Lepidoptera: Lymantriidae). Turk J Biol 36: 459–468.
  • Demir İ, Sezen K, Demirbağ Z (2002). Te frst study on bacterial fora and biological control agent of Anoplus roboris (Coleoptera: Curculionidae). J Microbiol 40: 104–108.
  • Demirci M, Sevim E, Demir İ, Sevim A (2013). Culturable bacterial microbiota of Plagiodera versicolora (L.) (Coleoptera: Chrysomelidae) and virulence of the isolated strains. Folia Microbiol 58: 201–210.
  • Dugas JE, Zurek L, Paster BJ, Keddie BA, Leadbetter ER (2001). Isolation and characterization of a Chryseobacterium strain from the gut of the American cockroach, Periplaneta americana. Arch Microbiol 175: 259–262.
  • El-Guindy MA, Abdel-Sattar MM, El-Refai ARM (1983). Te pattern of cross-resistance to insecticides and juvenile hormone analogues in a difubenzuron-resistant strain of the cotton leaf worm  Spodoptera littoralis Boisd. Pestic Sci 14: 235–245.
  • Farag NA (2008). Susceptibility of the cotton leafworm, Spodoptera littoralis 3rd instar larvae to some bio-insecticides (Lepidoptera: Noctuidae). Egypt J Biol Pest Co 18: 343–346.
  • Geiger A, Fardeau ML, Grebaut P, Vatunga G, Nando OJ, Herder SP, Cuny GR, Truc P, Ollivier B (2009). First isolation of Enterobacter, Enterococcus, and Acinetobacter spp. as inhabitants of the tsetse fly (Glossina palpalis) midgut. Infect Genet Evol 9: 1364–1370.
  • Gökçe C, Sevim A, Demirbağ Z, Demir İ (2010). Isolation, characterization and pathogenicity of bacteria from Rhynchites bacchus (Coleoptera: Rhynchitidae). Biocont Sci Technol 20: 973–982.
  • Hall TA (1999). BioEdit: A user-friendly biological sequence alignment editor and analysis program for windows 95/98/Nt. Nucleic Acids Symp 41: 95–98.
  • İnce İA, Katı H, Yılmaz H, Demir İ, Demirbağ Z (2008). Isolation and identifcation of bacteria from Taumetopoea pityocampa Den. and Schif. (Lepidoptera: Taumetopoeidae) and determination of their biocontrol potential. World J Microbiol Biotechnol 24: 3005–3015.
  • Inglis GD, Lawrence AM, Davis FM (2000). Pathogens associated with southwestern corn borers and southern corn stalk borers (Lepidoptera: Crambidae). J Econ Entomol 93: 1619–1626.
  • Jander G, Rahme LG, Ausubel FM (2000). Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects. J Bacteriol 182: 3843–3845.
  • Kamel AS, Aziz MFA, El-Barky NM (2010). Biochemical efects of three commercial formulations of Bacillus thuringiensis (Agerin, Dipel 2X and Dipel DF) on Spodoptera littoralis larvae. Egypt Acad J Biolog Sci 3: 21–29.
  • Khetan S (2001). Microbial Pest Control. New York: Marcel Dekker. Krieg NR, Holt JG (1986). Gram-negative aerobic rods and cocci. In: Palleroni NJ, editor. Bergey’s Manual of Systematic Bacteriology. Baltimore, MD, USA: Williams and Wilkins, pp. 140–218.
  • Lauzon CR, Bussert TG, Sjogren RE, Prokopy RJ (2003). Serratia marcescens as a bacterial pathogen of Rhagoletis pomonella fies (Diptera: Tephritidae). Eur J Entomol 100: 87–92.
  • Lee KP, Cory JS, Wilson K, Raubenheimer D, Simpson SJ (2006). Flexible diet choice ofsets protein costs of pathogen resistance in a caterpillar. Proc Biol Sci 273: 823–829.
  • Li H, Medina F, Vinson SB, Coates CJ (2005). Isolation, characterization, and molecular identifcation of bacteria from the red imported fre ant (Solenopsis invicta) midgut. J Invertebr Pathol 89: 203–209.
  • Martins T, Oliveira L, Garcia P (2005). Larval mortality factors of Spodoptera littoralis in the Azores. BioControl 50: 761–770.
  • Masetti A, De Luigi V, Burgio G (2008). Efects of nucleopolyhedrovirus based product on Spodoptera littoralis. Bull Insectol 61: 299–302.
  • Ministry of Agriculture of Turkey (2008). Te Technical Guide of Agricultural Control. Ankara: Başak Publisher (in Turkish). Moar WJ, Pusztzai-Carey M, Mack TP (1995). Toxicity of purifed proteins and the HD-1 strain from Bacillus thuringiensis against lesser cornstalk borer (Lepidoptera: Pyralidae). J Econ Entomol 88: 606–609.
  • Mosallanejad H, Smagghe G (2009). Biochemical mechanisms of methoxyfenozide resistance in the cotton leafworm Spodoptera littoralis. Pest Manag Sci 65: 732–736.
  • Navon A, Wysoki M, Keren S (1983). Potency and efect of Bacillus thuringiensis preparations against larvae of Spodoptera littoralis and Boarmia (Ascotis) selenaria . Phytoparasitica 11: 3–11.
  • Osborn F, Berlioz L, Vitelli-Flores J, Monsalve W, Dorta B, Lemoine VR (2002). Pathogenic efects of bacteria isolated from larvae of Hylesia metabus Crammer (Lepidoptera: Saturniidae). J Invertebr Pathol 80: 7–12.
  • Ravensberg WJ (2011). A Roadmap to the Successful Development and Commercialization of Microbial Pest Control Products for Control of Arthropods (Progress in Biological Control). Dordrecht: Springer.
  • Salama HS, Foda MS, Sharaby A (1989). A proposed new biological standard for bioassay of bacterial insecticides versus Spodoptera spp. Trop Pest Manage 35: 326–330.
  • Sambrook J, Fritsch EF, Maniatis T (1989). Molecular cloning: A Laboratory Manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Laboratory Press.
  • Seçil ES, Sevim A, Demirbağ, Z, Demir İ (2012). Isolation, characterization and virulence of bacteria from Ostrinia nubilalis (Lepidoptera: Pyralidae). Biologia 67: 767–776.
  • Sevim A, Demirbağ Z, Demir İ (2010). A new study on the bacteria of Agrotis segetum Schif. (Lepidoptera: Noctuidae) and their insecticidal activities. Turk J Agric For 34: 333–342.
  • Sevim A, Eryüzlü E, Demirbağ Z, Demir İ (2012). A Novel cry2Ab gene from the indigenous isolate Bacillus thuringiensis subsp. kurstaki. J Microbiol Biotechnol 22: 137–144.
  • Sezen K, Demir İ, Demirbağ Z (2004). Study of the bacterial fora as a biological control agent of Agelastica alni L. (Coleoptera: Chrysomelidae). Biologia 59: 327–331.
  • Sezen K, Demir İ, Demirbağ Z (2007). Identifcation and pathogenicity of entomopathogenic bacteria from common cockchafer, Melolontha melolontha (Coleoptera: Scarabaeidae) New Zeal J Crop Hort Sci 35: 79–85.
  • Sezen K, Demir İ, Katı H, Demirbağ Z (2005). Investigations on the bacteria as potential biological control agent of summer chafer, Amphimallon solstitiale L. (Coleoptera: Scarabaeidae). J Microbiol 43: 463–468.
  • Sezen K, Demirbağ Z (1999). Isolation and insecticidal activity of some bacteria from the hazelnut beetle (Balanicus nucum L.). Appl Entomol Zool 34: 85–89.
  • Sezen K, Muratoğlu H, Katı H, Nalçacıoğlu R, Mert D, Demirbağ Z (2008). A highly pathogenic Bacillus thuringiensis subsp. tenebrionis from European shot-hole borer, Xyleborus dispar Fabricius (Coleoptera: Scolytidae). New Zeal J Crop Hort 36: 77–84.
  • Sikorowski PP, Lawrence AM, Inglis GD (2001). Efects of Serratia marcescens on the rearing of the tobacco budworm (Lepidoptera: Noctuidae). Am Entomol 47: 51–60.
  • Sneath PHA, Mair NS, Sharpe ME, Holt JG (1986). Regular, nonsporing gram-positive rods. In: Kandler O, Weiss N, editors. Bergey’s Manual of Systematic Bacteriology. Baltimore, MD, USA: Williams and Wilkins, pp. 1208–1260.
  • Stahly DP, Andrews RE, Yousten AA (2006). Te genus Bacillus- insect pathogens. Prokaryotes 4: 563–608.
  • Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28: 2731–2739. 
  • Wegensteiner R (2007). Pathogens in bark beetles. In: Lieutier F, Day KR, Battisti A, Grégoire JC, Evans HF, editors. Bark and Wood Boring Insects in Living Trees in Europe: A Synthesis. Dordrecht: Springer, pp. 291–314.
  • Weisburg WG, Barns SM, Pelletier DA, Lane DJ (1991). 16s ribosomal DNA amplication for phylogenetic study. J Bacteriol 173: 697–703.
  • Yılmaz S, Karabörklü S, Azizoğlu U, Ayvaz A, Akbulut M, Yıldız M (2013). Toxicity of native Bacillus thuringiensis isolates on the larval stages of pine processionary moth Taumetopoea wilkinsoni at diferent temperatures. Turk J Agric For 37: 163– 172.
  • Yu H, Wang Z, Liu L, Xia Y, Cao Y, Yin Y (2008). Analysis of intestinal microfora in Hepialus gonggaensis (Lepidoptera: Hepialidae). Curr Microbiol 56: 391–396.
APA ÇAKICI ÖZKAN F, SEVİM A, DEMİRBAĞ Z, Demir İ (2014). Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. , 0 - 0.
Chicago ÇAKICI ÖZKAN Filiz,SEVİM Ali,DEMİRBAĞ ZİHNİ,Demir İsmail Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. (2014): 0 - 0.
MLA ÇAKICI ÖZKAN Filiz,SEVİM Ali,DEMİRBAĞ ZİHNİ,Demir İsmail Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. , 2014, ss.0 - 0.
AMA ÇAKICI ÖZKAN F,SEVİM A,DEMİRBAĞ Z,Demir İ Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. . 2014; 0 - 0.
Vancouver ÇAKICI ÖZKAN F,SEVİM A,DEMİRBAĞ Z,Demir İ Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. . 2014; 0 - 0.
IEEE ÇAKICI ÖZKAN F,SEVİM A,DEMİRBAĞ Z,Demir İ "Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents." , ss.0 - 0, 2014.
ISNAD ÇAKICI ÖZKAN, Filiz vd. "Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents". (2014), 0-0.
APA ÇAKICI ÖZKAN F, SEVİM A, DEMİRBAĞ Z, Demir İ (2014). Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. Turkish Journal of Agriculture and Forestry, 38(1), 0 - 0.
Chicago ÇAKICI ÖZKAN Filiz,SEVİM Ali,DEMİRBAĞ ZİHNİ,Demir İsmail Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. Turkish Journal of Agriculture and Forestry 38, no.1 (2014): 0 - 0.
MLA ÇAKICI ÖZKAN Filiz,SEVİM Ali,DEMİRBAĞ ZİHNİ,Demir İsmail Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. Turkish Journal of Agriculture and Forestry, vol.38, no.1, 2014, ss.0 - 0.
AMA ÇAKICI ÖZKAN F,SEVİM A,DEMİRBAĞ Z,Demir İ Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. Turkish Journal of Agriculture and Forestry. 2014; 38(1): 0 - 0.
Vancouver ÇAKICI ÖZKAN F,SEVİM A,DEMİRBAĞ Z,Demir İ Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents. Turkish Journal of Agriculture and Forestry. 2014; 38(1): 0 - 0.
IEEE ÇAKICI ÖZKAN F,SEVİM A,DEMİRBAĞ Z,Demir İ "Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents." Turkish Journal of Agriculture and Forestry, 38, ss.0 - 0, 2014.
ISNAD ÇAKICI ÖZKAN, Filiz vd. "Investigating internal bacteria of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) larvae and some Bacillus strains as biocontrol agents". Turkish Journal of Agriculture and Forestry 38/1 (2014), 0-0.
Sayfa Oluşturulma Tarihi
22-04-2024 20:30

İLETİŞİM

TÜBİTAK ULAKBİM TR Dizin

Yüzüncüyıl, İşçi Blokları Mahallesi

Muhsin Yazıcıoğlu Caddesi No:51/C

06530 Çankaya / ANKARA +90 (312) 298 92 00

trdizin@tubitak.gov.tr

TÜBİTAK ULAKBİM Ulusal Akademik Ağ ve Bilgi Merkezi Cahit Arf Bilgi Merkezi © Tüm Hakları Saklıdır. Gizlilik Politikası Kullanım Şartları