Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis

YAĞCI, Mehmet A.; YALTA, Tülin Deniz; OĞUZ, SERHAT; ÇOPUROĞLU, Elif; SAĞIROĞLU, Gönül; SAGIROGLU, TAMER; Sayhan, Mustafa Burak

Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis

Tamer SAĞIROĞLU, (Trakya Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Edirne, Türkiye)

Mustafa Burak SAYHAN, (Trakya Üniversitesi, Tıp Fakültesi, Acil Tıp Anabilim Dalı, Edirne, Türkiye)

Mehmet A. YAĞCI, (İnönü Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Malatya, Türkiye)

Tülin Deniz YALTA, (Trakya Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Edirne, Türkiye)

Gönül SAĞIROĞLU, (Trakya Üniversitesi, Tıp Fakültesi, Patoloji Anabilim Dalı, Edirne, Türkiye)

Elif ÇOPUROĞLU, (Trakya Üniversitesi, Tıp Fakültesi, Anesteziyoloji Anabilim Dalı, Edirne, Türkiye)

Serhat OĞUZ (Trakya Üniversitesi, Tıp Fakültesi, Genel Cerrahi Anabilim Dalı, Edirne, Türkiye)

Balkan Medical Journal

0 0

Yıl: 2015 Cilt: 32 Sayı: 1 Sayfa Aralığı: 101 - 106 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis

Öz:

Background: Continuous ambulatory peritoneal dialysis is a successful treatment modality for patients with end-stage renal disease. Peritoneal fibrosis (PF) is the most critical complication of long-term peritoneal di- alysis (PD). Aims: In our study, we aimed to compare the effects of colchicine and sirolimus on PF induced by hypertonic peritoneal dialysis solutions in rats. Study Design: Animal experiment. Methods: Twenty-four rats were randomly divided into three groups. The control group received an intraperitoneal injection (ip) of saline. The sirolimus group received the PD solution, plus 1.0 mg/kg/day Rapamune®. The colchicine group received the PD solution ip plus 1.0 mg/kg/day of colchicine. Blood sam- ples were taken to measure the serum levels of VEGF, TGF-β, and TNF-α. Peritoneal tissue samples were taken for histopathological evaluation. Results: TGF-β and TNF-α values in the sirolimus group were found to be statistically significantly lower than in the control and colchicine groups, but the differences between the control and colchicine groups were not statistically significant. No statistically significant differences were found between the groups regarding the VEGF values. Vascular neogenesis and peritoneal thickness were compared; the values in the sirolimus group were statistically reduced compared to the values in the control group. Mild fibrosis developed in 75% of all animals in the sirolimus group; there was no moderate or severe fibrosis observed. Fibrosis developed to varying degrees in 100% of the animals in the control and colchicine groups. Conclusion: The present study demonstrates that sirolimus might be beneficial for preventing or delaying the progression of PF and neoangiogenesis. These alterations in the peritoneal membrane may be connected with reduced TNF-α and TGF-β levels.

Anahtar Kelime:

Konular: Cerrahi

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

1.Sayarlioglu H, Dogan E, Erkoc R, Ozbek H, Bayram I, Sayar- lioglu M, et al. The effect of colchicine on the peritoneal mem- brane. Ren Fail 2006;28:69-75. [CrossRef]
2.Kawaguchi Y, Kawanishi H, Mujais S, Topley N, Oreopoulos DG. Encapsulating peritoneal sclerosis: definition, etiology, diagnosis and treatment. International Society for Peritoneal Dialysis Ad Hoc Committee on Ultrafiltration Management in Peritoneal Dialysis. Perit Dial Int 2000;20:43-55.
3. Van Westrhenen R, Aten J, Hajji N, de Boer OJ, Kunne C, de Waart DR, et al. Cyclosporin A induces peritoneal fibrosis and angiogen- esis during chronic peritoneal exposure to a glucose-based, lactate- buffered dialysis solution in the rat. Blood Purif 2007;25:466-72. [CrossRef]
4. Sayarlioglu H, Topal C, Sayarlioglu M, Dulger H, Dogan E, Erkoc R. Effect of glucose concentration on peritoneal inflam- matory cytokines in continuous ambulatory peritoneal dialysis patients. Mediators Inflamm 2004;13:119-21. [CrossRef]
5. Lin CY, Chen WP, Yang LY, Chen A, Huang TP. Persistent trans- forming growth factor beta 1 expression may predict peritoneal fibrosis in CAPD patients with frequent peritonitis occurrence. Am J Nephrol 1998;18:513-9. [CrossRef]
6. Margetts PJ, Oh KH, Kolb M. Transforming growth factor-beta: importance in long-term peritoneal membrane changes. Perit Dial Int 2005;25:15-7.
7. Douvdevani A, Rapoport J, Konforti A, Zlotnik M, Chaimovitz C. The effect of peritoneal dialysis fluid on the release of IL-1 beta and TNF alpha by macrophages/monocytes. Perit Dial Int 1993;13:112-7.
8. Fealy MJ, Umansky WS, Bickel KD, Nino JJ, Morris RE, Press BH. Efficacy of rapamycin and FK 506 in prolonging rat hind limb allograft survival. Ann Surg 1994;219:88-93. [CrossRef]
9. Duman S, Bozkurt D, Sipahi S, Sezak M, Ozkan S, Ertilav M, et al. Effects of everolimus as an antiproliferative agent on regres- sion of encapsulating peritoneal sclerosis in a rat model. Adv Perit Dial 2008;24:104-10.
10. Roqué M, Reis ED, Cordon-Cardo C, Taubman MB, Fallon JT, Fuster V, et al. Effect of p27 deficiency and rapamycin on inti- mal hyperplasia: in vivo and in vitro studies using a p27 knock- out mouse model. Lab Invest 2001;81:895-903. [CrossRef]
11. van Westrhenen R, Aten J, Aberra M, Dragt CA, Deira G, Kredi- et RT. Effects of inhibition of the polyol pathway during chronic peritoneal exposure to a dialysis solution. Perit Dial Int 2005;25 Suppl 3:18-21.
12. Kalk P, Rückert M, Godes M, von Websky K, Relle K, Neu- mayer HH, Hocher B, Morgera S. Does endothelin B recep- tor deficiency ameliorate the induction of peritoneal fibrosis in experimental peritoneal dialysis? Nephrol Dial Transplant 2010;25:1474-8. [CrossRef]
13. Yuan J, Fang W, Ni Z, Dai H, Lin A, Cao L, et al. Peritoneal morphologic changes in a peritoneal dialysis rat model corre- late with angiopoietin/Tie-2. Pediatr Nephrol 2009;24:163-70. [CrossRef]
14. Komatsu H, Uchiyama K, Tsuchida M, Isoyama N, Matsumura M, Hara T, et al. Development of a peritoneal sclerosis rat model using a continuous-infusion pump. Perit Dial Int 2008;28:641-7.
15. Grassmann A, Gioberge S, Moeller S, Brown G. ESRD pa- tients in 2004: global overview of patient numbers, treatment modalities and associated trends. Nephrol Dial Transplant 2005;20:2587-93. [CrossRef]
16. Devuyst O, Margetts PJ, Topley N. The pathophysiology of the peri- toneal membrane. J Am Soc Nephrol 2010;21:1077-85. [CrossRef]
17. Sampimon DE, Kolesnyk I, Korte MR, Fieren MW, Struijk DG, Krediet RT. Use of angiotensin II inhibitors in patients that develop encapsulating peritoneal sclerosis. Perit Dial Int 2010;30:656-9. [CrossRef]
18. Noh H, Ha H, Yu MR, Kim YO, Kim JH, Lee HB. Angiotensin II mediates high glucose-induced TGF-beta1 and fibronectin up- regulation in HPMC through reactive oxygen species. Perit Dial Int 2005;25:38-47.
19. Dainese L, Cappai A, Biglioli P. Recurrent pericardial effusion after cardiac surgery: the use of colchicine after recalcitrant con- ventional therapy. J Cardiothorac Surg 2011;10;6:96.
20. Kershenobich D, Vargas F, Garcia-Tsao G, Perez Tamayo R, Gent M, Rojkind M. Colchicine in the treatment of cirrhosis of the liver. N Engl J Med 1988;318:1709-13. [CrossRef]
21. Kaplan MM, Alling DW, Zimmerman HJ, Wolfe HJ, Sepersky RA, Hirsch GS, et al. A prospective trial of colchicine for primary biliary cirrhosis. N Engl J Med 1986;315:1448-54. [CrossRef]
22. Peters SG, McDougall JC, Douglas WW, Coles DT, DeRemee RA. Colchicine in the treatment of pulmonary fibrosis. Chest 1993;103:101-4. [CrossRef]
23. Lloberas N, Cruzado JM, Franquesa M, Herrero-Fresneda I, Torras J, Alperovich G, et al. Mammalian target of rapamycin pathway blockade slows progression of diabetic kidney disease in rats. J Am Soc Nephrol 2006;17:1395-404. [CrossRef]
24. Hartford CM, Ratain MJ. Rapamycin: something old, something new, sometimes borrowed and now renewed. Clin Pharmacol Ther 2007;82:381-8. [CrossRef]
25. Korfhagen TR, Le Cras TD, Davidson CR, Schmidt SM, Ike- gami M, Whitsett JA, et al. Rapamycin prevents transforming growth factor-alpha-induced pulmonary fibrosis. Am J Respir Cell Mol Biol 2009;41:562-72. [CrossRef]
26. Temple S, Zaltzman J, Perl J. Development of encapsulating peritoneal sclerosis in a renal transplant recipient on sirolimus immunotherapy. Perit Dial Int 2010;30:475-7. [CrossRef]
27. Lloberas N, Cruzado JM, Franquesa M, Herrero-Fresneda I, Torras J, Alperovich G, et al. Mammalian target of rapamycin pathway blockade slows progression of diabetic kidney disease in rats. J Am Soc Nephrol 2006;17:1395-404. [CrossRef]
28. Kramer S, Wang-Rosenke Y, Scholl V, Binder E, Loof T, Khadzhynov D, et al. Low-dose mTOR inhibition by rapamycin attenuates progression in anti-thy1- induced chronic glomerulo- sclerosis of the rat. Am J Physiol Renal Physiol 2008;294:F440-9. [CrossRef]
29. Peker K, Inal A, Sayar I, Sahin M, Gullu H, Inal DG, et al. Prevention of intraabdominal adhesions by local and systemic administration of immunosuppressive drugs. Iran Red Crescent Med J 2013;15:e14148. [CrossRef]
30. Parsons CJ, Takashima M, Rippe RA. Molecular mechanisms of hepatic fibrogenesis. J Gastroenterol Hepatol 2007;22:79-84. [CrossRef]

APA	SAGIROGLU T, Sayhan M, YAĞCI M, YALTA T, SAĞIROĞLU G, ÇOPUROĞLU E, OĞUZ S (2015). Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. , 101 - 106.
Chicago	SAGIROGLU TAMER,Sayhan Mustafa Burak,YAĞCI Mehmet A.,YALTA Tülin Deniz,SAĞIROĞLU Gönül,ÇOPUROĞLU Elif,OĞUZ SERHAT Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. (2015): 101 - 106.
MLA	SAGIROGLU TAMER,Sayhan Mustafa Burak,YAĞCI Mehmet A.,YALTA Tülin Deniz,SAĞIROĞLU Gönül,ÇOPUROĞLU Elif,OĞUZ SERHAT Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. , 2015, ss.101 - 106.
AMA	SAGIROGLU T,Sayhan M,YAĞCI M,YALTA T,SAĞIROĞLU G,ÇOPUROĞLU E,OĞUZ S Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. . 2015; 101 - 106.
Vancouver	SAGIROGLU T,Sayhan M,YAĞCI M,YALTA T,SAĞIROĞLU G,ÇOPUROĞLU E,OĞUZ S Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. . 2015; 101 - 106.
IEEE	SAGIROGLU T,Sayhan M,YAĞCI M,YALTA T,SAĞIROĞLU G,ÇOPUROĞLU E,OĞUZ S "Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis." , ss.101 - 106, 2015.
ISNAD	SAGIROGLU, TAMER vd. "Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis". (2015), 101-106.

APA	SAGIROGLU T, Sayhan M, YAĞCI M, YALTA T, SAĞIROĞLU G, ÇOPUROĞLU E, OĞUZ S (2015). Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. Balkan Medical Journal, 32(1), 101 - 106.
Chicago	SAGIROGLU TAMER,Sayhan Mustafa Burak,YAĞCI Mehmet A.,YALTA Tülin Deniz,SAĞIROĞLU Gönül,ÇOPUROĞLU Elif,OĞUZ SERHAT Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. Balkan Medical Journal 32, no.1 (2015): 101 - 106.
MLA	SAGIROGLU TAMER,Sayhan Mustafa Burak,YAĞCI Mehmet A.,YALTA Tülin Deniz,SAĞIROĞLU Gönül,ÇOPUROĞLU Elif,OĞUZ SERHAT Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. Balkan Medical Journal, vol.32, no.1, 2015, ss.101 - 106.
AMA	SAGIROGLU T,Sayhan M,YAĞCI M,YALTA T,SAĞIROĞLU G,ÇOPUROĞLU E,OĞUZ S Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. Balkan Medical Journal. 2015; 32(1): 101 - 106.
Vancouver	SAGIROGLU T,Sayhan M,YAĞCI M,YALTA T,SAĞIROĞLU G,ÇOPUROĞLU E,OĞUZ S Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis. Balkan Medical Journal. 2015; 32(1): 101 - 106.
IEEE	SAGIROGLU T,Sayhan M,YAĞCI M,YALTA T,SAĞIROĞLU G,ÇOPUROĞLU E,OĞUZ S "Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis." Balkan Medical Journal, 32, ss.101 - 106, 2015.
ISNAD	SAGIROGLU, TAMER vd. "Comparison of sirolimus and colchicine treatment on the development of peritoneal fibrozis in rats having peritoneal dialysis". Balkan Medical Journal 32/1 (2015), 101-106.