An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells

TURAÇLAR, Nesrin; Polat, Fikriye; Bingöl, Günsel

An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells

Fikriye POLAT, (Kocaeli Üniversitesi, Eğitim Fakültesi, Kocaeli, Türkiye)

Günsel BİNGÖL, (Yıldırım Beyazıt Üniversitesi, Mühendislik ve Doğa Bilimleri Fakültesi, Ankara, Türkiye)

Nesrin TURAÇLAR (Selçuk Üniversitesi, Sağlık Hizmetleri Meslek Yüksek Okulu, Konya, Türkiye)

Kafkas Üniversitesi Veteriner Fakültesi Dergisi

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Yıl: 2014 Cilt: 20 Sayı: 4 Sayfa Aralığı: 527 - 531 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells

Öz:

Bu çalışma ile sentetik antioksidan ve yiyeceklerde katkı maddesi olarak kullanılan Bütil Hidroksitoluenin (BHT) rat kemik iliğinde mikronükleuslu polikromatik eritrositlerin sayılarında herhangi bir artışa neden olup olmadığı araştırıldı. Mısır yağında çözülen BHT, iki farklı zaman periyodu ve üç farklı dozda (125, 250, 500 mg/kg v.a.), 8-10 haftalık erkek ve dişi Wistar albino sıçanlara (n=36) intraperitonal olarak verildi. BHT muamelesinden 12 ve 24 saat sonra kemik iliği örnekleri MNPCE sayısı için analiz edildi. Ayrıca, polikromatik eritrositlerin normokromatik eritrositlere oranı (PCE/NCE) değerlendirilerek BHT’nin kemik iliğindeki sitotoksik etkisine bakıldı. Bütün BHT dozları ve iki farklı zaman periyodunda MNPCE’lerin sayısında 1.9- 2.84 kat önemli artışlar bulundu. Diğer taraftan BHT, kemik iliği sitotoksisite belirteci olan PCE/NCE oranını kontrol grubuna kıyasla düşürdü. Sonuç olarak bu çalışmada BHT’nin rat kemik iliğinde MNPCE oluşumunu artırdığını ve bu artışın, uygulanan dozlar ve verilme şekliyle ilişkili olabileceğini düşünmekteyiz.

Anahtar Kelime:

Konular: Veterinerlik

Bütil Hidroksitoluen tarafından mikronükleus indüksiyonunun wistar sıçan kemik iliği hücrelerinde araştırılması

Öz:

This study aims to investigate whether Butylated Hydroxytoluene (BHT), the synthetic antioxidant and food additive, increases the frequency of micronucleated polychromatic erythrocytes (MNPCEs) in rat bone marrow. BHT, dissolved in corn oil, was administered intraperitoneally to 8-10 week old male and female Wistar rats (n=36) in three different doses (125, 250 and 500 mg/kg b.w.) for two different time periods. 12- and 24-h after BHT treatment, the bone marrow samples were analyzed for the frequency of MNPCEs. Additionally, by evaluating the ratio of polychromatic erythrocyte to normochromatic erythrocyte (PCE/NCE), the cytotoxic effect of BHT on bone marrow was tested. It was found that all BHT doses at two time periods significantly increased the MNPCEs frequency about 1.9-2.84 fold. On the other hand, BHT caused significant decreased in the PCE/NCE ratio, which is indicative of bone marrow cytotoxicity when compared to the control groups. This study showed that BHT increased the formation of MNPCEs in rat bone marrow and we think that this increase might be related to the applied doses and the administration way BHT.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	Polat F, Bingöl G, TURAÇLAR N (2014). An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. , 527 - 531.
Chicago	Polat Fikriye,Bingöl Günsel,TURAÇLAR Nesrin An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. (2014): 527 - 531.
MLA	Polat Fikriye,Bingöl Günsel,TURAÇLAR Nesrin An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. , 2014, ss.527 - 531.
AMA	Polat F,Bingöl G,TURAÇLAR N An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. . 2014; 527 - 531.
Vancouver	Polat F,Bingöl G,TURAÇLAR N An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. . 2014; 527 - 531.
IEEE	Polat F,Bingöl G,TURAÇLAR N "An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells." , ss.527 - 531, 2014.
ISNAD	Polat, Fikriye vd. "An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells". (2014), 527-531.

APA	Polat F, Bingöl G, TURAÇLAR N (2014). An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20(4), 527 - 531.
Chicago	Polat Fikriye,Bingöl Günsel,TURAÇLAR Nesrin An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20, no.4 (2014): 527 - 531.
MLA	Polat Fikriye,Bingöl Günsel,TURAÇLAR Nesrin An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, vol.20, no.4, 2014, ss.527 - 531.
AMA	Polat F,Bingöl G,TURAÇLAR N An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(4): 527 - 531.
Vancouver	Polat F,Bingöl G,TURAÇLAR N An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells. Kafkas Üniversitesi Veteriner Fakültesi Dergisi. 2014; 20(4): 527 - 531.
IEEE	Polat F,Bingöl G,TURAÇLAR N "An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells." Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 20, ss.527 - 531, 2014.
ISNAD	Polat, Fikriye vd. "An investigation of micronucleus induction by Butylated Hydroxytoluene in wistar rat bone marrow cells". Kafkas Üniversitesi Veteriner Fakültesi Dergisi 20/4 (2014), 527-531.