Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk

KARA, RECEP; AKKAYA, Levent; GÖKMEN, Mukadderat; GÖK, Levent

Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk

Mukadderat GÖKMEN, (Balıkesir Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknoloji Anabilim Dalı, Balıkesir, Türkiye)

recep kara, (Afyon Kocatepe Üniversitesi, Veteriner Fakültesi, Besin Hijyeni Ve Teknolojisi Anabilim Dalı, Kars.)

Levent AKKAYA, (Balıkesir Üniversitesi, Veteriner Fakültesi, Besin Hijyeni ve Teknoloji Anabilim Dalı, Balıkesir, Türkiye)

Levent GÖK (Afyon Kocatepe Üniversitesi, Mühendislik Fakültesi, Gıda Mühendisliği Bölümü, Afyon, Türkiye)

Eurasian Journal of Veterinary Sciences

4 0

Yıl: 2013 Cilt: 29 Sayı: 4 Sayfa Aralığı: 192 - 197 Metin Dili: Türkçe İndeks Tarihi: 29-07-2022

Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk

Öz:

Amaç: Bu çalışmada, Afyonkarahisar'da beş farklı işletmede üretilen Türk sucuğu örneklerinde Enterobacteriaceae ve Staphylococcaceae familyalarına ait türlerin identifikasyonu yapıldı.Gereç ve Yöntem: Materyal olarak Afyonkarahisar'ın beş farklı bölgesinde üretilen Türk fermente sucukları kullanıldı. Enterobacteriaceae sayımı için Violet Red Bile Glucose Agar ve (ISO 21528-2 2004), Staphylococcaceae sayımı için Baird Parker Agar (FDA BAM 2001) kullanıldı. Enterobacteriaceae tür düzeyinde API-20E test kiti (bioMérieux Inc API- 20 E 2006) ile Staphylococcaceae tür düzeyinde API-Staph test kiti (bioMérieux Inc API-Staph 2009) ile identifikasyonu yapıldı.Bulgular: Türk sucuklarında Staphylococcaceae ve Enterobacteriaceae türlerinin izolasyon ve idetifikasyonu yapıldı. Araştırmada 90 Staphylococcus izolatı Türk sucuklarından izole edildi. İzole edilen Staphylococcaceae izolatları S. aureus (%12.94), S. epidermidis (%15.29), S. simulans (%12.94) ve S. carnosus (%11.76) olarak identifiye edildi. İzole edilen Enterobacteriaceae izolatları ise Citrobacter diversus (%5.96), Enterobacter sazazaki (%11.91), Enterobacter gergoviae (%17.87), Klepsiella ozaerae (%6.38), Salmonella arizonae (%6.38), Salmonella spp (%8.94), Escherichia coli (%8.94), Serratia marcescens (%6.81), Serratia liquefaciens (%6.38) ve Yersinia enterocolitica (%4.26) olarak identifiye edildi.Öneri: Türk sucuklarında Staphylococcaceae familyasına ait starter kültür olarak kullanılabilecek S. simulans ilk defa tespit edilmiştir. Ayrıca Türk sucuklarında tespit edilen Staphylococcaceae ve Enterobacteriaceae familyasına ait patojen bakteri türlerinin gıda güvenliği ve halk sağlığı açısından risk taşıdığını göstermektedir.

Anahtar Kelime:

Konular: Veterinerlik

Türk sucuğunda Enterobacteriaceae ve Staphylococcaceae identifikasyonu

Öz:

Aim: In this study, the species from Enterobacteriaceae and Staphylococcaceae families have been identified in Turkish fermented sausages samples of five different corporations which are produced in Afyonkarahisar.Materials and Methods: Turkish fermented sausages of five different corporations which are produced in Afyonkarahisar were used as the material. In order for the calculation of Enterobacteriaceae, inoculation was made into Violet Red Bile Glucose Agar medium (ISO 21528-2 2004) and for the calculation of Staphylococcaceae Baird Parker Agar medium (FDA BAM, 2001) was used. Enterobacteriaceae were identified at species level with API- 20 E test kit (bioMérieux Inc API-20 E 2006). Staphylococcaceae were identified with APIStaph test kit (bioMérieux Inc API-Staph 2009).Results: Isolation and identification of Staphylococcaceae and Enterobacteriaceae species in Turkish sausage flora were done. 90 Staphylococcus isolates were derived from the Turkish sausage samples in the research. S. aureus (12.94%), S. epidermidis (15.29%), S. simulans (12.94%) and S. carnosus (11.76%) from Staphylococcaceae family have been identified. 235 Enterobacteriaceae isolates from Turkish sausage samples were identified at the species level as Citrobacter diversus (5.96%), Enterobacter sazazaki (11.91%), Enterobacter gergoviae (17.87%), Klepsiella ozaerae (6.38%), Salmonella arizonae (6.38%), Salmonella spp. (8.94%), Escherichia coli (8.94%), Serratia marcescens (6.81%), Serratia liquefaciens (6.38%) and Yersinia enterocolitica (4.26%).Conclusion: S. simulans from Staphylococcaceae family which can be used as a starter culture has been found in Turkish sausages for the first time. Pathogenic bacterium species from Staphylococcaceae and Enterobacteriaceae families determined in Turkish sausages indicate a risk for food safety and public health.

Anahtar Kelime:

Konular: Veterinerlik

Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık

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APA	GÖKMEN M, KARA R, AKKAYA L, GÖK L (2013). Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. , 192 - 197.
Chicago	GÖKMEN Mukadderat,KARA RECEP,AKKAYA Levent,GÖK Levent Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. (2013): 192 - 197.
MLA	GÖKMEN Mukadderat,KARA RECEP,AKKAYA Levent,GÖK Levent Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. , 2013, ss.192 - 197.
AMA	GÖKMEN M,KARA R,AKKAYA L,GÖK L Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. . 2013; 192 - 197.
Vancouver	GÖKMEN M,KARA R,AKKAYA L,GÖK L Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. . 2013; 192 - 197.
IEEE	GÖKMEN M,KARA R,AKKAYA L,GÖK L "Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk." , ss.192 - 197, 2013.
ISNAD	GÖKMEN, Mukadderat vd. "Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk". (2013), 192-197.

APA	GÖKMEN M, KARA R, AKKAYA L, GÖK L (2013). Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences, 29(4), 192 - 197.
Chicago	GÖKMEN Mukadderat,KARA RECEP,AKKAYA Levent,GÖK Levent Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences 29, no.4 (2013): 192 - 197.
MLA	GÖKMEN Mukadderat,KARA RECEP,AKKAYA Levent,GÖK Levent Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences, vol.29, no.4, 2013, ss.192 - 197.
AMA	GÖKMEN M,KARA R,AKKAYA L,GÖK L Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences. 2013; 29(4): 192 - 197.
Vancouver	GÖKMEN M,KARA R,AKKAYA L,GÖK L Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk. Eurasian Journal of Veterinary Sciences. 2013; 29(4): 192 - 197.
IEEE	GÖKMEN M,KARA R,AKKAYA L,GÖK L "Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk." Eurasian Journal of Veterinary Sciences, 29, ss.192 - 197, 2013.
ISNAD	GÖKMEN, Mukadderat vd. "Identification of Enterobacteriaceae and Staphylococcaceae at Turkish sucuk". Eurasian Journal of Veterinary Sciences 29/4 (2013), 192-197.