Yıl: 2014 Cilt: 38 Sayı: 3 Sayfa Aralığı: 307 - 317 Metin Dili: İngilizce İndeks Tarihi: 29-07-2022

Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey

Öz:
In this study, 26 Pseudomonas spp. were isolated from a stream polluted by factory waste and from petroleum-contaminated soil. The surface tension (ST) of the cultures was used as a criterion for the primary isolation of biosurfactant-producing bacteria. Biosurfactant production was quantified by ST reduction, critical micelle concentration (CMC), emulsification capacity (EC), and cell surface hydrophobicity (CSH). Two of the isolates, P. aeruginosa 78 and 99, produced rhamnolipid biosurfactant. The strains started rhamnolipid production in the logarithmic phase. They decreased the ST of the culture from 73 dyne/cm2 to 29 and 33 dyne/cm2, and the CMC of produced rhamnolipids were 115 and 130 mg/L, respectively. P. aeruginosa 78 and 99 strains emulsified benzene and n-hexane at the highest rates, and the surfaces of these strains were 73% and 65% and 62% and 72% more hydrophobic for benzene and toluene, respectively.
Anahtar Kelime:

Konular: Biyoloji
Belge Türü: Makale Makale Türü: Araştırma Makalesi Erişim Türü: Erişime Açık
  • Antunes AA, Silva MLRB, Silva CAA, Campos-Takaki GM (2006). Characterization of Chromobacterium violaceum isolated from Paca River, Pernambuco, Brazil. Revista de Biologia E Ciências da Terra 1: 29–39.
  • Arutchelvi J, Joseph C, Doble M (2011). Process optimization for the production of rhamnolipid and formation of biofilm by Pseudomonas aeruginosa CPCL on polypropylene. Biochemical Engineering Journal 56: 37–45.
  • Atlas RM, Parks LC (1997). Handbook of Microbiological Media. 2nd ed. New York, NY, USA: CRC Press.
  • Banat IM, Makkar RS, Cameotra SS (2000). Potential commercial applications of microbial surfactants. Appl Microbiol Biotechnol 53: 495–508.
  • Beal R, Betts WB (2000). Role of rhamnolipid biosurfactants in the uptake and mineralization of hexadecane in Pseudomonas aeruginosa. J Appl Microbiol 89: 158–168.
  • Bodour AA, Drees KP, Maier RM (2003). Distribution of biosurfactant-producing bacteria in undisturbed and contaminated arid Southwestern soils. Appl Environ Microb 69: 3280–3287.
  • Calvo C, Martínez-Checa F, Toledo FL, Porcel J, Quesada E (2002). Characteristics of bioemulsifiers synthesised in crude oil media by Halomonas eurihalina and their effectiveness in the isolation of bacteria able to grow in the presence of hydrocarbons. Appl Microbiol Biotechnol 60: 347–351.
  • Cassidy DP, Hudak AJ Jr, Werkema DD, Atekwana EA, Rossbach S, Duris JD, Atekwana EA, Sauck WS (2002). In situ rhamnolipid production at an abandoned petroleum refinery. Soil Sediment Contam 11: 769–787.
  • Caykara T, Birlik G (2005). Swelling and adsorption properties of hydrophobic poly[(n- (3-(dimethylamino)propyl) methacrylamide)-co-(lauryl acrylate)] hydrogels in aqueous solutions of surfactants. Macromol Mater Eng 290: 869–874.
  • Celik GY, Aslim B, Beyatli Y (2008). Enhanced crude oil biodegradation and rhamnolipid production by Pseudomonas stutzeri strain G11 in the presence of Tween-80 and Triton X-100. J Environ Biol 29: 867–870.
  • Christofi N, Ivshina IB (2002). Microbial surfactants and their use in field studies of soil remediation. J Appl Microbiol 93: 915–929.
  • Cohen R, Ozdemir G, Exerowa D (2003). Free thin liquid films (foam films) from rhamnolipids: type of the film and stability. Colloids and Surfaces B: Biointerfaces 29: 197–204.
  • Costa SGVAO, Nitschke M, Haddad R, Eberlin MN, Contiero J (2005). Production of Pseudomonas aeruginosa LBI rhamnolipids following growth on Brazilian native oil. Process Biochem 41: 483–488.
  • Cui X (2004). Regulation of biosurfactant production by quorum sensing in Pseudomonas fluorescens 5064, the cause of broccoli head rot disease. PhD, The University of Edinburgh, Edinburgh, United Kingdom.
  • Delden CV, Iglew BH (1998). Cell-to-cell signaling and Pseudomonas aeruginosa infections. Emerg Infect Dis 4: 551–560.
  • Desai JD, Banat IM (1997). Microbial production of surfactants and their commercial potential. Microbiol Mol Biol R 61: 47–64.
  • Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956). Colorimetric method for determination of sugars and related substances. Anal Chem 28: 350–356.
  • Georgiou G, Lin S, Sharma MM (1992). Surface-active compounds from microorganisms. Bio/Technology 10: 60–65.
  • Gogra AB, Yao J, Sandy EH, Zheng SX, Zaray G, Koroma BM, Hui Z (2010). Cell surface hydrophobicity (CSH) of Escherichia coli, Staphylococcus aureus and Aspergillus niger and the biodegradation of diethyl phthalate (DEP) via microcalorimetry. Journal of American Science 6: 78–88.
  • Guerra-Santos LH, Käppeli O, Fiechter A (1986). Dependence of Pseudomonas aeruginosa continuous culture biosurfactant production on nutritional and environmental factors. Appl Microbiol Biot 24: 443–448.
  • Hamme JDV, Singh A, Ward OP (2006). Physiological aspects. Part 1 in a series of papers devoted to surfactants in microbiology and biotechnology. Biotechnol Adv 24: 604–620.
  • Itoh S, Honda H, Tomita F, Suzuki T (1971). Rhamnolipid produced by Pseudomonas aeruginosa grown on n-paraffin. J Antibiot 24: 855–859.
  • Khalladi R, Benhabiles O, Bentahar F, Moulai-Mostefa N (2009). Surfactant remediation of diesel fuel polluted soil. J Hazard Mater 164: 1179–1184.
  • Khoshdast H, Sam A, Vali H, Noghabi KA (2011). Effect of rhamnolipid biosurfactants on performance of coal and mineral flotation. International Biodeterioration & Biodegradation 65: 1238–1243.
  • Kosaric N, Cairns WL, Gray NCC (1987). Biosurfactants and Biotechnology. New York, NY, USA: Marcel Dekker Inc.
  • Kosaric N (1992). Biosurfactants in industry. Pure Appl Chem 64: 1731–1737.
  • Kosaric N (2001). Biosurfactants and their application for soil bioremediation. Food Technol Biotechnol 39: 295–304.
  • Laemmli UK (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.
  • Lang S, Wullbrandt D (1999). Rhamnose lipids-biosynthesis microbial production and application potential. Appl Microbiol Biot 51: 22–32.
  • Long X, Zhang G, Han L, Meng Q (2013a). Dewatering floated oily sludge by treatment with rhamnolipid. Water Research 47: 4303–4311.
  • Long X, Zhang G, Shen C, Sun G, Wang R, Yin L, Meng Q (2013b). Application of rhamnolipid as a novel biodemulsifier for destabilizing waste crude oil. Bioresource Technology 131: 1–5.
  • Lotfabad TB, Shourian M, Roostaazad R, Najafabadi AR, Adelzadeh MR, Noghabi KA (2009). An efficient biosurfactant-producing bacterium Pseudomonas aeruginosa MR01, isolated from oil excavation areas in south of Iran. Colloids and Surfaces B: Biointerfaces 69: 183–193.
  • Maier RM, Soberon-Chavez G (2000). Pseudomonas aeruginosa rhamnolipids: biosynthesis and potential applications. Appl Microbiol Biot 54: 625–633.
  • Makkar RS, Cameotra SS (1999). Biosurfactant production by microorganisms on unconventional carbon sources. J Surf Det 2: 237–241.
  • Maneerat S (2005). Production of biosurfactants using substrates from renewable-resources. Songklanakarin J Sci Technol 27: 675–683.
  • Maneerat S (2005). Biosurfactants from marine microorganisms. Songklanakarin J Sci Technol 27: 1263–1272.
  • Margesin R, Schinner F (2001). Bioremediation (natural attenuation and biostimulation) of diesel-oil contaminated soil in an alpine glacier skiing area. Appl Environ Microb 67: 3127–3133.
  • Mata-Sandoval JC, Karns J, Torrents A (1999). High-performance liquid chromatography method for the characterization of rhamnolipid mixtures produced by Pseudomonas aeruginosa UG2 on corn oil. J Chromatogr A 864: 211–220.
  • Moraes IO, Benincasa M, Alegre RM (2002). Production and characterization of rhamnolipids produced by a newly isolated strain of Pseudomonas aeruginosa . Braz J Food Technol 5: 145–149.
  • Nayak AS, Vijaykumar MH, Karegoudar TB (2009). Characterization of biosurfactant produced by Pseudoxanthomonas sp. PNK-04 and its application in bioremediation. Int Biodeter Biodegr 63: 73–79.
  • Noordman WH, Janssen DB (2002). Rhamnolipid stimulates uptake of hydrophobic compounds by Pseudomonas aeruginosa. Appl Environ Microb 68: 4502–4508.
  • Patel RM, Desai AJ (1997). Biosurfactant production by Pseudomonas aeruginosa GS3 from molasses. Lett Appl Microbiol 25: 91–94.
  • Onbasli D, Aslim B (2009). Biosurfactant production in sugar beet molasses by some Pseudomonas spp. J Environ Biol 30: 161–163.
  • Ozturk S, Kaya T, Aslim B, Tan S (2012). Removal and reduction of chromium by Pseudomonas spp. and their correlation to rhamnolipid production. Journal of Hazardous Materials 231– 232: 64–69.
  • Pornsunthorntawee O, Chavadej S, Rujiravanit R (2009). Solution properties and vesicle formation of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa SP4. Colloids and Surfaces B: Biointerfaces 7: 26–15.
  • Rashedi H, Jamshidi E, Assadi M, Bonakdarpour B (2005). Isolation and production of biosurfactant from Pseudomonas aeruginosa isolated from Iranian southern wells oil. Int J Environ Sci Tech 2: 121–127.
  • Raza ZA, Rehman A, Khan MS, Khalid ZM (2007). Improved production of biosurfactant by a Pseudomonas aeruginosa mutant using vegetable oil refinery wastes. Biodegradation 18: 115–121.
  • Rosenberg M, Gutnick D, Rosenberg E (1980). Adherence of bacteria to hydrocarbons: a simple method for measuring cell-surface hydrophobicity. FEMS Microbiol Lett 9: 29–33.
  • Santa Anna LMS, Sebastian GV, Menezes EP, Alves TLM, Santos AS, Pereira N Jr, Freire DMG (2002). Production of biosurfactants from Pseudomonas aeruginosa PA1 isolated in oil environments.
  • Brazilian Journal of Chemical Engineering 19: 159–166. Silva GP, Mack M, Contiero J (2009). Glycerol: a promising and abundant carbon source for industrial microbiology. Biotechnol Adv 27: 30–39.
  • Tabatabaee A, Assadi MM, Noohi AA, Sajadian VA (2005). Isolation of biosurfactant producing bacteria from oil reservoirs. Iranian J Env Health Sci Eng 2: 6–12.
  • Tuleva BK, Ivanov GR, Christova NE (2002). Biosurfactant production by a new Pseudomonas putida strain. Verlag der Zeitschrift für Naturforschung Tübingen 57: 356–360.
  • Whang LM, Liu PW, Ma CC, Cheng SS (2009). Application of rhamnolipid and surfactin for enhanced diesel biodegradation—effects of pH and ammonium addition. J Hazard Mater 164: 1045–1050.
  • Wong JWC, Fang M, Zhao Z, Xing B (2003). Effect of surfactants on solubilization and degradation of phenanthrene under thermophilic conditions. J Environ Qual 33: 2015–2025.
  • Youssef NH, Duncan KE, McInerney MJ (2005). Importance of 3-hydroxy fatty acid composition of lipopeptides for biosurfactant activity. Appl Environ Microb 71: 7690–7695.
  • Zhang G, Wu Y, Qian X, Meng Q (2005). Biodegradation of crude oil by Pseudomonas aeruginosa in the presence of rhamnolipids. J Zhejiang Univ SCI 6: 725–730.
  • Zhang Y, Miller RM (1995). Effect of rhamnolipid (biosurfactant) structure on solubilization and biodegradation of n-alkanes. Appl Environ Microb 61: 2247–2251.
  • Zita A, Hermansson M (1997). Determination of bacterial cell surface hydrophobicity of single cells in cultures and in wastewater in situ. FEMS Microbiol Letters 152: 299–306.
APA Kaya T, Aslim B, KARİPTAŞ E (2014). Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. , 307 - 317.
Chicago Kaya Tayfun,Aslim Belma,KARİPTAŞ Engin Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. (2014): 307 - 317.
MLA Kaya Tayfun,Aslim Belma,KARİPTAŞ Engin Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. , 2014, ss.307 - 317.
AMA Kaya T,Aslim B,KARİPTAŞ E Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. . 2014; 307 - 317.
Vancouver Kaya T,Aslim B,KARİPTAŞ E Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. . 2014; 307 - 317.
IEEE Kaya T,Aslim B,KARİPTAŞ E "Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey." , ss.307 - 317, 2014.
ISNAD Kaya, Tayfun vd. "Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey". (2014), 307-317.
APA Kaya T, Aslim B, KARİPTAŞ E (2014). Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. Turkish Journal of Biology, 38(3), 307 - 317.
Chicago Kaya Tayfun,Aslim Belma,KARİPTAŞ Engin Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. Turkish Journal of Biology 38, no.3 (2014): 307 - 317.
MLA Kaya Tayfun,Aslim Belma,KARİPTAŞ Engin Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. Turkish Journal of Biology, vol.38, no.3, 2014, ss.307 - 317.
AMA Kaya T,Aslim B,KARİPTAŞ E Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. Turkish Journal of Biology. 2014; 38(3): 307 - 317.
Vancouver Kaya T,Aslim B,KARİPTAŞ E Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey. Turkish Journal of Biology. 2014; 38(3): 307 - 317.
IEEE Kaya T,Aslim B,KARİPTAŞ E "Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey." Turkish Journal of Biology, 38, ss.307 - 317, 2014.
ISNAD Kaya, Tayfun vd. "Production of biosurfactant by Pseudomonas spp. isolated from industrial waste in Turkey". Turkish Journal of Biology 38/3 (2014), 307-317.